Rhodoplanes sp. Z2-YC6860
Average proteome isoelectric point is 7.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7007 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A127F2H4|A0A127F2H4_9RHIZ Transglutaminase family protein OS=Rhodoplanes sp. Z2-YC6860 OX=674703 GN=RHPLAN_53590 PE=4 SV=1
MM1 pKa = 7.41 ATQSTGGGSTTSFGNAPQAVDD22 pKa = 3.69 DD23 pKa = 4.73 TFTSAQTGLTEE34 pKa = 4.92 DD35 pKa = 3.84 LLKK38 pKa = 10.84 VVYY41 pKa = 10.19 LDD43 pKa = 4.61 VMANDD48 pKa = 4.2 LGGNAKK54 pKa = 8.39 TLFSIDD60 pKa = 3.0 NGIYY64 pKa = 10.01 TGTTVQSDD72 pKa = 4.59 LLTQDD77 pKa = 3.25 TARR80 pKa = 11.84 AEE82 pKa = 4.14 ATSADD87 pKa = 3.8 TSLKK91 pKa = 9.86 GAKK94 pKa = 8.94 IWITSDD100 pKa = 3.07 GKK102 pKa = 10.92 VGYY105 pKa = 9.4 DD106 pKa = 3.7 ASTLSQSFKK115 pKa = 10.79 DD116 pKa = 3.62 QLSALHH122 pKa = 6.38 AGEE125 pKa = 4.52 YY126 pKa = 9.51 LTDD129 pKa = 3.33 TFTYY133 pKa = 10.22 AIRR136 pKa = 11.84 LGNGTLSWATATVQFAGANDD156 pKa = 3.84 TATITASGNEE166 pKa = 4.08 DD167 pKa = 3.49 TTVVEE172 pKa = 5.15 SGGTNNTTLGDD183 pKa = 3.9 PSASGQLTVNDD194 pKa = 3.39 VDD196 pKa = 3.69 SGEE199 pKa = 4.21 NHH201 pKa = 6.07 FQTPSSLAGTYY212 pKa = 7.56 GTYY215 pKa = 10.13 TFNATSGVWGYY226 pKa = 7.48 TLNQSLADD234 pKa = 4.22 PLTAGQVVYY243 pKa = 10.97 DD244 pKa = 3.61 HH245 pKa = 7.16 LIVKK249 pKa = 9.91 SADD252 pKa = 3.01 GTASHH257 pKa = 7.67 DD258 pKa = 3.46 IAVKK262 pKa = 9.05 ITGSNDD268 pKa = 2.92 SATITASSTEE278 pKa = 3.99 DD279 pKa = 3.27 TSVKK283 pKa = 10.1 EE284 pKa = 4.06 AGGVSNGAAGDD295 pKa = 3.99 PSAHH299 pKa = 6.15 GQLTVHH305 pKa = 6.84 DD306 pKa = 4.15 VDD308 pKa = 3.83 SGEE311 pKa = 4.18 NHH313 pKa = 6.06 FQTPASLAGTYY324 pKa = 7.56 GTYY327 pKa = 10.11 TFNEE331 pKa = 4.36 TSGAWGYY338 pKa = 7.48 TLNQSLADD346 pKa = 4.02 SLTDD350 pKa = 3.5 GQVVHH355 pKa = 7.06 DD356 pKa = 4.18 HH357 pKa = 6.98 LIVKK361 pKa = 10.02 SADD364 pKa = 3.11 NTTSYY369 pKa = 11.08 DD370 pKa = 3.04 IDD372 pKa = 3.74 VTITGTNDD380 pKa = 2.91 AATITASATEE390 pKa = 4.03 DD391 pKa = 3.53 TSVKK395 pKa = 10.09 EE396 pKa = 4.06 AGGVSNGTAGDD407 pKa = 4.0 PSAHH411 pKa = 6.11 GQLTVHH417 pKa = 6.84 DD418 pKa = 4.15 VDD420 pKa = 3.83 SGEE423 pKa = 4.18 NHH425 pKa = 6.06 FQTPASLAGTYY436 pKa = 7.56 GTYY439 pKa = 10.11 TFNATTGAWSYY450 pKa = 9.67 TLNQSIADD458 pKa = 3.9 SLYY461 pKa = 10.5 DD462 pKa = 3.41 GQVAHH467 pKa = 7.2 DD468 pKa = 3.93 HH469 pKa = 6.53 LIVRR473 pKa = 11.84 SADD476 pKa = 3.06 NTASYY481 pKa = 10.28 DD482 pKa = 3.52 INIDD486 pKa = 3.28 ILGTNDD492 pKa = 3.37 AAVLSSASVNLTEE505 pKa = 5.42 GDD507 pKa = 3.5 TAADD511 pKa = 3.28 ISTSGTLTISDD522 pKa = 4.02 VDD524 pKa = 3.93 SDD526 pKa = 3.94 PHH528 pKa = 7.41 FVAQTDD534 pKa = 3.57 TAGNYY539 pKa = 10.28 GKK541 pKa = 10.63 FSIDD545 pKa = 3.9 ADD547 pKa = 4.3 GAWSYY552 pKa = 11.37 VADD555 pKa = 3.71 SAHH558 pKa = 6.42 NEE560 pKa = 3.9 FAAGQHH566 pKa = 4.67 YY567 pKa = 9.5 TEE569 pKa = 4.84 SFDD572 pKa = 4.0 VVSADD577 pKa = 3.51 GTHH580 pKa = 6.32 TSVAIDD586 pKa = 3.13 ILGTNDD592 pKa = 3.19 AATVAFDD599 pKa = 3.73 TVSFSDD605 pKa = 3.31 TGSNDD610 pKa = 3.15 GDD612 pKa = 3.64 HH613 pKa = 6.58 LTNNNAVTFSGSYY626 pKa = 10.42 SDD628 pKa = 3.99 VDD630 pKa = 3.64 GTVTQIQVFNGSTSLGFATLATGTWTLNTALVDD663 pKa = 3.89 GSYY666 pKa = 10.85 NQLRR670 pKa = 11.84 VLATDD675 pKa = 3.41 NNRR678 pKa = 11.84 ATTEE682 pKa = 4.07 ATNPTQIIVDD692 pKa = 3.95 TLNPTVSSVSINDD705 pKa = 3.47 NQITDD710 pKa = 3.35 GDD712 pKa = 3.96 AAGIRR717 pKa = 11.84 TATITFSEE725 pKa = 4.59 AMDD728 pKa = 3.54 QTSTPTISNSASSTLTSPTGGHH750 pKa = 5.61 WVDD753 pKa = 3.3 ATHH756 pKa = 6.64 YY757 pKa = 9.99 AINYY761 pKa = 5.83 TAADD765 pKa = 3.51 AGVTLNDD772 pKa = 2.73 ITFNVSGAKK781 pKa = 10.09 DD782 pKa = 3.3 LAGNTQAAATNVSSGTSVDD801 pKa = 3.55 TQNPTNTWSFSTSSKK816 pKa = 10.61 AITVTASDD824 pKa = 3.34 SSGIGSVTAVDD835 pKa = 3.72 NTHH838 pKa = 7.22 PGQGFAAAVNNGNGTWTIATSPSANINGDD867 pKa = 3.71 SVTVTVTDD875 pKa = 3.71 GAGNVTTSTHH885 pKa = 5.67 NAPAGVAGSLINLGLTDD902 pKa = 4.53 PSVDD906 pKa = 3.44 HH907 pKa = 6.94 IGAVSTVISGIPAGWILSEE926 pKa = 4.76 GADD929 pKa = 3.26 NGNGNWAVQTDD940 pKa = 3.75 NLAALSIISSAAFTGALVLNVTEE963 pKa = 4.26 TWTNADD969 pKa = 3.44 GSNGTSIVSDD979 pKa = 3.56 NVEE982 pKa = 4.03 VFATGSPIFAWSGDD996 pKa = 3.5 DD997 pKa = 3.93 HH998 pKa = 6.67 LTGSSGRR1005 pKa = 11.84 DD1006 pKa = 2.94 LFVFSQPIGADD1017 pKa = 3.34 VIHH1020 pKa = 6.69 NFDD1023 pKa = 3.87 AAADD1027 pKa = 3.9 QIDD1030 pKa = 4.64 LIDD1033 pKa = 3.6 YY1034 pKa = 9.59 HH1035 pKa = 8.06 SEE1037 pKa = 3.75 SGATLTYY1044 pKa = 11.1 ADD1046 pKa = 4.58 LQSLLSQDD1054 pKa = 3.47 GAGNAVISLGNGQSITLDD1072 pKa = 3.47 GVHH1075 pKa = 6.83 AADD1078 pKa = 3.78 LSEE1081 pKa = 4.53 ANFVFDD1087 pKa = 4.58 VTPTLDD1093 pKa = 3.25 NAGTMTIGDD1102 pKa = 4.06 GAMLPLSGIINNTGTIEE1119 pKa = 4.2 LDD1121 pKa = 3.47 SAGSSTLLQLIQYY1134 pKa = 8.84 GITLQGNGTVVLSDD1148 pKa = 3.19 NDD1150 pKa = 3.79 GNVISGSVQSVTLHH1164 pKa = 6.18 NLDD1167 pKa = 3.5 NTIEE1171 pKa = 4.31 GAGQLGAGQLDD1182 pKa = 4.04 LTNDD1186 pKa = 3.32 GTIIANGSHH1195 pKa = 6.8 ALVIDD1200 pKa = 3.64 TGANVIEE1207 pKa = 4.13 NAGMLEE1213 pKa = 3.96 ATGTGGLVINSALEE1227 pKa = 3.82 NDD1229 pKa = 4.12 GLVWAHH1235 pKa = 6.77 GGNITLSGAVTGSGSVQIDD1254 pKa = 3.36 GNAKK1258 pKa = 9.3 VSFGAVASVNTNIAADD1274 pKa = 3.71 SASTVTVHH1282 pKa = 7.89 DD1283 pKa = 4.05 SFDD1286 pKa = 3.68 FSGVISGFNGDD1297 pKa = 3.9 DD1298 pKa = 3.82 HH1299 pKa = 7.07 MDD1301 pKa = 3.42 LTDD1304 pKa = 3.3 VMFSAGLTVNYY1315 pKa = 9.73 AAAADD1320 pKa = 4.02 GTGGVLQVSDD1330 pKa = 4.18 GAHH1333 pKa = 5.67 TANIALLGQYY1343 pKa = 9.23 DD1344 pKa = 3.56 ASGFRR1349 pKa = 11.84 AGADD1353 pKa = 3.43 SSSGTSITYY1362 pKa = 9.94 DD1363 pKa = 3.21 PHH1365 pKa = 5.61 HH1366 pKa = 6.32 TVV1368 pKa = 3.04
Molecular weight: 138.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.694
IPC_protein 3.77
Toseland 3.516
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.923
Wikipedia 3.745
Rodwell 3.579
Grimsley 3.427
Solomon 3.783
Lehninger 3.732
Nozaki 3.884
DTASelect 4.215
Thurlkill 3.579
EMBOSS 3.757
Sillero 3.897
Patrickios 0.922
IPC_peptide 3.77
IPC2_peptide 3.872
IPC2.peptide.svr19 3.781
Protein with the highest isoelectric point:
>tr|A0A127F6W2|A0A127F6W2_9RHIZ Elongation factor P OS=Rhodoplanes sp. Z2-YC6860 OX=674703 GN=efp PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.65 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 LKK23 pKa = 7.72 TTGGRR28 pKa = 11.84 KK29 pKa = 8.99 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7007
0
7007
2322162
29
10376
331.4
35.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.2 ± 0.038
0.835 ± 0.009
5.432 ± 0.025
5.192 ± 0.031
3.764 ± 0.018
8.428 ± 0.032
2.043 ± 0.014
5.305 ± 0.02
4.035 ± 0.029
9.646 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.532 ± 0.016
2.86 ± 0.026
5.503 ± 0.023
3.24 ± 0.019
6.694 ± 0.039
5.504 ± 0.028
5.47 ± 0.048
7.65 ± 0.023
1.337 ± 0.011
2.33 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here