Lupinus angustifolius (Narrow-leaved blue lupine)
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 31106 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1J7FMY5|A0A1J7FMY5_LUPAN Glycosyltransferase family 92 protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_23887 PE=3 SV=1
MM1 pKa = 7.76 ADD3 pKa = 2.79 VTTYY7 pKa = 10.69 LRR9 pKa = 11.84 HH10 pKa = 6.25 HH11 pKa = 6.76 KK12 pKa = 9.93 PDD14 pKa = 3.57 EE15 pKa = 4.68 DD16 pKa = 4.19 FDD18 pKa = 6.28 SITLPYY24 pKa = 9.97 FSQSSPDD31 pKa = 3.59 FNFDD35 pKa = 3.88 FYY37 pKa = 11.45 SSEE40 pKa = 4.31 PEE42 pKa = 4.01 FPPTPEE48 pKa = 4.94 PDD50 pKa = 3.49 SLSLFDD56 pKa = 4.57 RR57 pKa = 11.84 HH58 pKa = 5.39 NQINFAIDD66 pKa = 3.54 IFQQCVDD73 pKa = 3.39 QSQVSGHH80 pKa = 6.3 GYY82 pKa = 9.93 FVTEE86 pKa = 4.54 PRR88 pKa = 11.84 NDD90 pKa = 3.74 DD91 pKa = 3.63 DD92 pKa = 5.43 FGVIDD97 pKa = 4.58 VYY99 pKa = 11.45 SALDD103 pKa = 3.34 MDD105 pKa = 5.88 DD106 pKa = 5.14 NLGLDD111 pKa = 3.45 LGFGVEE117 pKa = 4.18 RR118 pKa = 11.84 DD119 pKa = 3.55 GVSISEE125 pKa = 4.22 SNEE128 pKa = 3.81 APFHH132 pKa = 5.92 NCVCVMGLGSDD143 pKa = 3.42 SDD145 pKa = 3.89 EE146 pKa = 4.1 EE147 pKa = 4.32 EE148 pKa = 4.36 EE149 pKa = 4.17 EE150 pKa = 4.63 NEE152 pKa = 4.18 VLEE155 pKa = 4.32 NCVNCDD161 pKa = 3.21 EE162 pKa = 5.2 EE163 pKa = 5.05 YY164 pKa = 11.45 NDD166 pKa = 4.02 VNDD169 pKa = 4.63 DD170 pKa = 3.65 VLIIPLCWDD179 pKa = 3.52 SLQLDD184 pKa = 3.97 EE185 pKa = 5.78 DD186 pKa = 3.67 NKK188 pKa = 10.8 EE189 pKa = 4.11 RR190 pKa = 11.84 IEE192 pKa = 4.13 DD193 pKa = 4.08 FEE195 pKa = 4.4 WEE197 pKa = 4.23 EE198 pKa = 4.3 VNVDD202 pKa = 3.14 GRR204 pKa = 11.84 VDD206 pKa = 3.49 EE207 pKa = 5.06 RR208 pKa = 11.84 EE209 pKa = 4.18 VLSMLTIDD217 pKa = 4.63 DD218 pKa = 4.32 DD219 pKa = 5.27 RR220 pKa = 11.84 SVSVSGIPVIEE231 pKa = 4.29 EE232 pKa = 3.9 EE233 pKa = 4.38 EE234 pKa = 4.15 EE235 pKa = 4.15 DD236 pKa = 3.85 VSVVRR241 pKa = 11.84 MGGMEE246 pKa = 3.96 NLEE249 pKa = 4.05 WEE251 pKa = 4.61 VLLNANNLNTITSPDD266 pKa = 4.0 LDD268 pKa = 3.89 NDD270 pKa = 4.15 DD271 pKa = 5.48 DD272 pKa = 5.04 LEE274 pKa = 4.16 EE275 pKa = 5.31 PYY277 pKa = 10.75 FSDD280 pKa = 3.1 HH281 pKa = 7.71 DD282 pKa = 4.18 EE283 pKa = 4.53 YY284 pKa = 10.84 IYY286 pKa = 10.69 ATDD289 pKa = 3.68 YY290 pKa = 11.86 EE291 pKa = 4.67 MMFGQFVEE299 pKa = 4.08 NEE301 pKa = 4.01 YY302 pKa = 9.16 TLRR305 pKa = 11.84 PPASVSVVRR314 pKa = 11.84 NLPSVVVTKK323 pKa = 10.93 EE324 pKa = 4.2 DD325 pKa = 3.26 VDD327 pKa = 4.07 NNNALCAVCKK337 pKa = 10.5 DD338 pKa = 3.73 DD339 pKa = 5.22 FAVAEE344 pKa = 4.13 QAKK347 pKa = 9.56 QLPCSHH353 pKa = 7.38 RR354 pKa = 11.84 YY355 pKa = 9.56 HH356 pKa = 7.26 GDD358 pKa = 3.72 CIVPWLAIRR367 pKa = 11.84 NTCPVCRR374 pKa = 11.84 YY375 pKa = 7.43 EE376 pKa = 5.81 FPTDD380 pKa = 3.41 DD381 pKa = 3.87 ADD383 pKa = 3.92 YY384 pKa = 11.43 EE385 pKa = 4.44 RR386 pKa = 11.84 MRR388 pKa = 11.84 TNRR391 pKa = 11.84 SALRR395 pKa = 11.84 AA396 pKa = 3.67
Molecular weight: 45.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.757
IPC_protein 3.783
Toseland 3.567
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.605
Grimsley 3.465
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.088
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.897
Patrickios 1.189
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A1J7HC80|A0A1J7HC80_LUPAN AB hydrolase-1 domain-containing protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_24992 PE=4 SV=1
MM1 pKa = 7.09 FRR3 pKa = 11.84 VLEE6 pKa = 4.19 FQCFDD11 pKa = 3.21 GCGVEE16 pKa = 4.52 GRR18 pKa = 11.84 GSRR21 pKa = 11.84 VQGPRR26 pKa = 11.84 TRR28 pKa = 11.84 VYY30 pKa = 10.53 GLRR33 pKa = 11.84 SRR35 pKa = 11.84 VQGRR39 pKa = 11.84 GSRR42 pKa = 11.84 VQGPRR47 pKa = 11.84 STVHH51 pKa = 6.22 GPGSTVHH58 pKa = 6.94 GIGSRR63 pKa = 11.84 VQGPGSRR70 pKa = 11.84 VQGPGSRR77 pKa = 11.84 VQGPGSRR84 pKa = 11.84 VQGPGSRR91 pKa = 11.84 VQGPGSRR98 pKa = 11.84 VQGPGSRR105 pKa = 11.84 VQGPGSRR112 pKa = 11.84 VQGPGSRR119 pKa = 11.84 VQGPGSRR126 pKa = 11.84 VQGPGSRR133 pKa = 11.84 VQGPGSRR140 pKa = 11.84 VQGPGSRR147 pKa = 11.84 VQGPGSRR154 pKa = 11.84 VQGPGSRR161 pKa = 11.84 VQGPGSRR168 pKa = 11.84 VQGPGSRR175 pKa = 11.84 VQGPGSRR182 pKa = 11.84 VQGPGSRR189 pKa = 11.84 VQGPGSRR196 pKa = 11.84 VQGPGSRR203 pKa = 11.84 VQGPGSRR210 pKa = 11.84 VQGPGSRR217 pKa = 11.84 VQGPGSRR224 pKa = 11.84 VQGPGSRR231 pKa = 11.84 VQGPGSRR238 pKa = 11.84 VQGPGSRR245 pKa = 11.84 VQGPGSRR252 pKa = 11.84 VQGPGSRR259 pKa = 11.84 VQGPGSRR266 pKa = 11.84 VQGPGSRR273 pKa = 11.84 VQGPGSRR280 pKa = 11.84 VQGPGSRR287 pKa = 11.84 VQGPGSRR294 pKa = 11.84 VQGPGSRR301 pKa = 11.84 VQGPGSRR308 pKa = 11.84 VQGPGSRR315 pKa = 11.84 VQGPGSRR322 pKa = 11.84 VQGPGSRR329 pKa = 11.84 VQGPGSRR336 pKa = 11.84 VQGPGSRR343 pKa = 11.84 VQGPGSRR350 pKa = 11.84 VQGPGSRR357 pKa = 11.84 VQGPGSRR364 pKa = 11.84 VQGPGSRR371 pKa = 11.84 VQGPGSRR378 pKa = 11.84 VQGPGSRR385 pKa = 11.84 VQGPGSRR392 pKa = 11.84 VQGPGSRR399 pKa = 11.84 VQGPGSRR406 pKa = 11.84 VQGPGSRR413 pKa = 11.84 VQGPGSRR420 pKa = 11.84 VQGPGSRR427 pKa = 11.84 VQGPGSRR434 pKa = 11.84 VQGPGSRR441 pKa = 11.84 VQGPGSRR448 pKa = 11.84 VQGPGSRR455 pKa = 11.84 VQGPGSRR462 pKa = 11.84 VQGPGSRR469 pKa = 11.84 VQGPGSRR476 pKa = 11.84 VQGPGSRR483 pKa = 11.84 VQGPGSRR490 pKa = 11.84 VQGPGSRR497 pKa = 11.84 VQGPGSRR504 pKa = 11.84 VQGPGSRR511 pKa = 11.84 VQGPGSRR518 pKa = 11.84 VQGPGSRR525 pKa = 11.84 VQGPGSRR532 pKa = 11.84 VQGPGSRR539 pKa = 11.84 VQGPGSRR546 pKa = 11.84 VQGPGSRR553 pKa = 11.84 VQGPGSRR560 pKa = 11.84 VQGPGSRR567 pKa = 11.84 VQGPGSRR574 pKa = 11.84 VQGPGSRR581 pKa = 11.84 VQGPGSRR588 pKa = 11.84 VQGPGSRR595 pKa = 11.84 VQGPGSRR602 pKa = 11.84 VQGPGSRR609 pKa = 11.84 VQGPGSRR616 pKa = 11.84 VQGPGSRR623 pKa = 11.84 VQGPGSRR630 pKa = 11.84 VQGPGSRR637 pKa = 11.84 VQGPGSRR644 pKa = 11.84 VQGPGSRR651 pKa = 11.84 VQGPGSRR658 pKa = 11.84 VQGPGSRR665 pKa = 11.84 VQGPGSRR672 pKa = 11.84 VQGPGSRR679 pKa = 11.84 VQGPGSRR686 pKa = 11.84 VQGPGSRR693 pKa = 11.84 VQGPGSRR700 pKa = 11.84 VQGPGSRR707 pKa = 11.84 VQGPGSRR714 pKa = 11.84 VQGPGSRR721 pKa = 11.84 VQGPGSRR728 pKa = 11.84 VQGPGSRR735 pKa = 11.84 VQGPGSRR742 pKa = 11.84 VQGPGSRR749 pKa = 11.84 VQGPGSRR756 pKa = 11.84 VQGPGSRR763 pKa = 11.84 VQGPGSRR770 pKa = 11.84 VQGPGSRR777 pKa = 11.84 VQGPGSRR784 pKa = 11.84 VQGPGSRR791 pKa = 11.84 VQGPGSRR798 pKa = 11.84 VQGPGSRR805 pKa = 11.84 VQGPGSRR812 pKa = 11.84 VQGPGSRR819 pKa = 11.84 VQGPGSRR826 pKa = 11.84 VQGPGSRR833 pKa = 11.84 VQGPGSRR840 pKa = 11.84 VQGPGSRR847 pKa = 11.84 VQGPGSRR854 pKa = 11.84 VQGPGSRR861 pKa = 11.84 VQGPGSRR868 pKa = 11.84 VQGPGSRR875 pKa = 11.84 VQGPGSRR882 pKa = 11.84 VQGPGSRR889 pKa = 11.84 VQGPGSRR896 pKa = 11.84 VQGPGSRR903 pKa = 11.84 VQGPGSRR910 pKa = 11.84 VQGPGSRR917 pKa = 11.84 VQGPGSRR924 pKa = 11.84 VQGPGSRR931 pKa = 11.84 VQGPGSRR938 pKa = 11.84 VQGPGSRR945 pKa = 11.84 VQGPGSRR952 pKa = 11.84 VQGPGSRR959 pKa = 11.84 VQGPGSRR966 pKa = 11.84 VQGPGSRR973 pKa = 11.84 VQGPGSRR980 pKa = 11.84 VQGPGSRR987 pKa = 11.84 VQGPGSRR994 pKa = 11.84 VQGPGSRR1001 pKa = 11.84 VQGPGSRR1008 pKa = 11.84 VQGPGSRR1015 pKa = 11.84 VQGPGSRR1022 pKa = 11.84 VQGPGSRR1029 pKa = 11.84 VQGPGSRR1036 pKa = 11.84 VQGPGSRR1043 pKa = 11.84 VQGPGSRR1050 pKa = 11.84 VQGPGSRR1057 pKa = 11.84 VQGPGSRR1064 pKa = 11.84 VQGPGSRR1071 pKa = 11.84 VQGPGSRR1078 pKa = 11.84 VQGPGSRR1085 pKa = 11.84 VQGPGSRR1092 pKa = 11.84 VQGPGSRR1099 pKa = 11.84 VQGPGSRR1106 pKa = 11.84 VQGPGSRR1113 pKa = 11.84 VQGPGSRR1120 pKa = 11.84 VQGPGSRR1127 pKa = 11.84 VQGPGSRR1134 pKa = 11.84 VQGPGSRR1141 pKa = 11.84 VQGPGSRR1148 pKa = 11.84 VQGPGSRR1155 pKa = 11.84 VQGPGSRR1162 pKa = 11.84 VQGPGSRR1169 pKa = 11.84 VQGPGSRR1176 pKa = 11.84 VQGPGSRR1183 pKa = 11.84 VQGPGSRR1190 pKa = 11.84 VQGPGSRR1197 pKa = 11.84 VQGPGSRR1204 pKa = 11.84 VQGPGSRR1211 pKa = 11.84 VQGPGSRR1218 pKa = 11.84 VQGPGSRR1225 pKa = 11.84 VQGPGSRR1232 pKa = 11.84 VQGPGSRR1239 pKa = 11.84 VQGPGSRR1246 pKa = 11.84 VQGPGSRR1253 pKa = 11.84 VQGPGSRR1260 pKa = 11.84 VQGPGSRR1267 pKa = 11.84 VQGPGSRR1274 pKa = 11.84 VQGPGSRR1281 pKa = 11.84 NYY1283 pKa = 10.89
Molecular weight: 125.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.468
IPC2_protein 11.623
IPC_protein 13.188
Toseland 13.349
ProMoST 13.846
Dawson 13.349
Bjellqvist 13.349
Wikipedia 13.832
Rodwell 12.852
Grimsley 13.378
Solomon 13.846
Lehninger 13.744
Nozaki 13.349
DTASelect 13.349
Thurlkill 13.349
EMBOSS 13.846
Sillero 13.349
Patrickios 12.544
IPC_peptide 13.846
IPC2_peptide 12.837
IPC2.peptide.svr19 9.466
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
31096
10
31106
13402789
49
5685
430.9
48.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.449 ± 0.013
1.745 ± 0.007
5.389 ± 0.012
6.518 ± 0.022
4.151 ± 0.01
6.434 ± 0.015
2.534 ± 0.006
5.463 ± 0.01
6.276 ± 0.015
9.382 ± 0.016
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.393 ± 0.006
4.903 ± 0.013
4.929 ± 0.02
3.728 ± 0.01
4.975 ± 0.011
9.129 ± 0.018
5.011 ± 0.008
6.543 ± 0.01
1.21 ± 0.005
2.838 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here