Flavobacterium caeni
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3216 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G5GHP1|A0A1G5GHP1_9FLAO Starch-binding associating with outer membrane OS=Flavobacterium caeni OX=490189 GN=SAMN02927903_01579 PE=3 SV=1
MM1 pKa = 7.0 MRR3 pKa = 11.84 IFLFAAIGLLTACDD17 pKa = 4.34 KK18 pKa = 11.24 DD19 pKa = 4.15 DD20 pKa = 3.95 QNAMPYY26 pKa = 9.47 YY27 pKa = 9.71 VAPAGTADD35 pKa = 3.49 VTLTMTGGSTYY46 pKa = 10.63 EE47 pKa = 4.05 LDD49 pKa = 4.72 GPCGWAYY56 pKa = 10.61 AGGVGYY62 pKa = 10.79 VGANQTDD69 pKa = 3.49 NSLKK73 pKa = 10.02 TFSIDD78 pKa = 3.22 TNLTEE83 pKa = 4.69 LPSVTTTFTLTDD95 pKa = 3.86 DD96 pKa = 5.21 ALDD99 pKa = 4.13 EE100 pKa = 5.26 DD101 pKa = 4.16 PTKK104 pKa = 11.14 ARR106 pKa = 11.84 IHH108 pKa = 5.31 LTEE111 pKa = 4.03 FVGSSFVSWDD121 pKa = 3.09 STTASGQVTFAVNGNEE137 pKa = 4.01 VTVDD141 pKa = 3.3 LSGITLEE148 pKa = 4.79 AEE150 pKa = 4.26 SSNDD154 pKa = 3.16 VPYY157 pKa = 11.1 DD158 pKa = 3.65 NNGTLSGTLKK168 pKa = 10.51 FYY170 pKa = 10.84 KK171 pKa = 10.46
Molecular weight: 18.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.795
IPC_protein 3.783
Toseland 3.567
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.617
Grimsley 3.478
Solomon 3.77
Lehninger 3.732
Nozaki 3.91
DTASelect 4.164
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.91
Patrickios 0.947
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.804
Protein with the highest isoelectric point:
>tr|A0A1G5KNJ3|A0A1G5KNJ3_9FLAO Addiction module antidote protein HigA family OS=Flavobacterium caeni OX=490189 GN=SAMN02927903_03385 PE=4 SV=1
MM1 pKa = 7.52 ILRR4 pKa = 11.84 YY5 pKa = 9.18 IYY7 pKa = 10.38 KK8 pKa = 8.67 PSRR11 pKa = 11.84 RR12 pKa = 11.84 ITAASLHH19 pKa = 6.31 CGVFRR24 pKa = 11.84 GRR26 pKa = 11.84 KK27 pKa = 8.71 FPFHH31 pKa = 7.29 EE32 pKa = 4.49 EE33 pKa = 4.08 TLSSAEE39 pKa = 4.13 RR40 pKa = 11.84 TQSAA44 pKa = 3.51
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.736
IPC_protein 10.613
Toseland 10.496
ProMoST 10.277
Dawson 10.657
Bjellqvist 10.409
Wikipedia 10.891
Rodwell 10.804
Grimsley 10.73
Solomon 10.774
Lehninger 10.745
Nozaki 10.511
DTASelect 10.409
Thurlkill 10.54
EMBOSS 10.906
Sillero 10.584
Patrickios 10.657
IPC_peptide 10.774
IPC2_peptide 9.575
IPC2.peptide.svr19 8.362
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3216
0
3216
1096505
36
2774
341.0
38.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.868 ± 0.051
0.831 ± 0.017
5.647 ± 0.034
5.685 ± 0.052
5.188 ± 0.042
6.93 ± 0.053
1.904 ± 0.022
6.803 ± 0.041
6.599 ± 0.075
8.972 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.276 ± 0.025
5.555 ± 0.042
3.822 ± 0.033
3.855 ± 0.026
3.746 ± 0.039
6.041 ± 0.037
6.4 ± 0.093
6.8 ± 0.035
1.123 ± 0.017
3.956 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here