Pelargonium chlorotic ring pattern virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Pelarspovirus

Average proteome isoelectric point is 8.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6EN28|Q6EN28_9TOMB RNA-directed RNA polymerase OS=Pelargonium chlorotic ring pattern virus OX=167021 GN=RdRp PE=4 SV=1
MM1 pKa = 7.85DD2 pKa = 6.22PSPPSADD9 pKa = 2.77ITSTQQNQKK18 pKa = 10.09RR19 pKa = 11.84GKK21 pKa = 9.51AKK23 pKa = 10.21NRR25 pKa = 11.84LDD27 pKa = 3.63VAHH30 pKa = 6.74SGVSKK35 pKa = 10.91ASNSDD40 pKa = 3.39LVGANFITVAEE51 pKa = 4.34TVNFTVHH58 pKa = 7.0LNFDD62 pKa = 3.53WGLASPNLLLFITFNFFLVSVFSVGDD88 pKa = 3.5RR89 pKa = 11.84YY90 pKa = 11.08SYY92 pKa = 11.04YY93 pKa = 10.57YY94 pKa = 9.74QANSTADD101 pKa = 3.29KK102 pKa = 9.92FISVAVGKK110 pKa = 10.29

Molecular weight: 12.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6EN27|Q6EN27_9TOMB Isoform of Q6EN28 27 kDa putative replicase protein OS=Pelargonium chlorotic ring pattern virus OX=167021 GN=RdRp PE=4 SV=1
MM1 pKa = 7.7GGTPEE6 pKa = 5.14DD7 pKa = 4.17SEE9 pKa = 4.73NCSSRR14 pKa = 11.84SPSCFPNPLSPNKK27 pKa = 9.93ALRR30 pKa = 11.84FSGVRR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.79PAPLLWNFPRR47 pKa = 11.84VASPALTSSS56 pKa = 3.29

Molecular weight: 6.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

2

6

1566

56

763

261.0

29.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.663 ± 0.83

2.363 ± 0.618

4.534 ± 0.339

5.428 ± 0.814

5.3 ± 0.452

6.769 ± 0.458

2.043 ± 0.453

4.342 ± 0.394

6.45 ± 0.431

7.727 ± 0.342

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.107 ± 0.198

4.47 ± 0.546

4.853 ± 0.664

3.321 ± 0.452

6.45 ± 0.601

8.046 ± 1.284

6.003 ± 0.785

8.11 ± 0.401

1.213 ± 0.303

2.746 ± 0.404
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski