Lactococcus phage 98201
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B1IN15|A0A1B1IN15_9CAUD dUTPase OS=Lactococcus phage 98201 OX=1871690 GN=DS98201_17 PE=4 SV=1
MM1 pKa = 7.43 KK2 pKa = 10.25 CKK4 pKa = 10.51 KK5 pKa = 9.82 CGNKK9 pKa = 9.03 IDD11 pKa = 4.34 CDD13 pKa = 3.72 CMGCHH18 pKa = 6.09 EE19 pKa = 4.92 CHH21 pKa = 6.92 PEE23 pKa = 4.08 YY24 pKa = 10.65 TCEE27 pKa = 4.03 TCGFCHH33 pKa = 6.81 IDD35 pKa = 2.97 GWEE38 pKa = 4.39 AGACWSLANDD48 pKa = 4.05 PDD50 pKa = 4.18 YY51 pKa = 11.63 DD52 pKa = 3.94 PFDD55 pKa = 3.3 II56 pKa = 5.77
Molecular weight: 6.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.391
IPC2_protein 4.444
IPC_protein 4.304
Toseland 4.139
ProMoST 4.469
Dawson 4.304
Bjellqvist 4.457
Wikipedia 4.24
Rodwell 4.151
Grimsley 4.05
Solomon 4.291
Lehninger 4.253
Nozaki 4.431
DTASelect 4.635
Thurlkill 4.19
EMBOSS 4.253
Sillero 4.444
Patrickios 0.057
IPC_peptide 4.291
IPC2_peptide 4.431
IPC2.peptide.svr19 4.344
Protein with the highest isoelectric point:
>tr|A0A1B1IN16|A0A1B1IN16_9CAUD Uncharacterized protein OS=Lactococcus phage 98201 OX=1871690 GN=DS98201_22 PE=4 SV=1
MM1 pKa = 7.31 FSKK4 pKa = 11.2 NEE6 pKa = 3.43 IRR8 pKa = 11.84 RR9 pKa = 11.84 GDD11 pKa = 3.85 KK12 pKa = 10.16 ICFRR16 pKa = 11.84 DD17 pKa = 3.7 TKK19 pKa = 9.46 FLKK22 pKa = 10.75 VIEE25 pKa = 4.37 VTDD28 pKa = 3.67 KK29 pKa = 11.35 YY30 pKa = 10.43 ITVEE34 pKa = 3.72 KK35 pKa = 10.93 DD36 pKa = 2.88 QFTKK40 pKa = 10.79 KK41 pKa = 10.01 SVKK44 pKa = 9.95 RR45 pKa = 11.84 DD46 pKa = 3.15 DD47 pKa = 3.68 FRR49 pKa = 11.84 IVKK52 pKa = 10.18 INGRR56 pKa = 11.84 YY57 pKa = 8.05 HH58 pKa = 6.28 ACEE61 pKa = 3.69 LFDD64 pKa = 4.44 RR65 pKa = 11.84 VVKK68 pKa = 10.8
Molecular weight: 8.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.946
IPC2_protein 8.96
IPC_protein 8.916
Toseland 9.94
ProMoST 9.575
Dawson 10.072
Bjellqvist 9.692
Wikipedia 10.175
Rodwell 10.687
Grimsley 10.101
Solomon 10.116
Lehninger 10.101
Nozaki 9.984
DTASelect 9.663
Thurlkill 9.955
EMBOSS 10.321
Sillero 9.999
Patrickios 10.467
IPC_peptide 10.116
IPC2_peptide 8.244
IPC2.peptide.svr19 7.907
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
52
0
52
11315
52
869
217.6
24.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.46 ± 0.448
0.557 ± 0.137
6.204 ± 0.237
7.0 ± 0.617
4.445 ± 0.269
6.849 ± 0.493
1.335 ± 0.196
7.114 ± 0.476
7.972 ± 0.438
7.592 ± 0.391
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.36 ± 0.205
6.01 ± 0.315
3.084 ± 0.147
4.092 ± 0.204
3.668 ± 0.327
7.141 ± 0.304
7.247 ± 0.629
6.01 ± 0.224
1.29 ± 0.138
3.57 ± 0.226
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here