Amino acid dipepetide frequency for Streptomyces phage Annadreamy

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.729AlaAla: 7.729 ± 0.92
0.784AlaCys: 0.784 ± 0.17
4.397AlaAsp: 4.397 ± 0.35
5.881AlaGlu: 5.881 ± 0.509
3.165AlaPhe: 3.165 ± 0.274
5.517AlaGly: 5.517 ± 0.588
1.316AlaHis: 1.316 ± 0.178
4.033AlaIle: 4.033 ± 0.339
4.677AlaLys: 4.677 ± 0.38
7.001AlaLeu: 7.001 ± 0.461
2.632AlaMet: 2.632 ± 0.39
3.053AlaAsn: 3.053 ± 0.343
2.436AlaPro: 2.436 ± 0.292
3.193AlaGln: 3.193 ± 0.56
4.061AlaArg: 4.061 ± 0.378
4.117AlaSer: 4.117 ± 0.419
4.957AlaThr: 4.957 ± 0.562
4.733AlaVal: 4.733 ± 0.393
1.596AlaTrp: 1.596 ± 0.206
2.772AlaTyr: 2.772 ± 0.318
0.0AlaXaa: 0.0 ± 0.0
Cys
0.448CysAla: 0.448 ± 0.112
0.196CysCys: 0.196 ± 0.068
0.952CysAsp: 0.952 ± 0.188
0.56CysGlu: 0.56 ± 0.132
0.364CysPhe: 0.364 ± 0.126
1.456CysGly: 1.456 ± 0.216
0.392CysHis: 0.392 ± 0.118
0.476CysIle: 0.476 ± 0.107
0.952CysLys: 0.952 ± 0.181
0.644CysLeu: 0.644 ± 0.161
0.28CysMet: 0.28 ± 0.092
0.532CysAsn: 0.532 ± 0.131
0.588CysPro: 0.588 ± 0.154
0.336CysGln: 0.336 ± 0.117
0.532CysArg: 0.532 ± 0.108
0.532CysSer: 0.532 ± 0.121
0.532CysThr: 0.532 ± 0.126
0.476CysVal: 0.476 ± 0.118
0.224CysTrp: 0.224 ± 0.083
0.224CysTyr: 0.224 ± 0.083
0.0CysXaa: 0.0 ± 0.0
Asp
5.237AspAla: 5.237 ± 0.392
0.728AspCys: 0.728 ± 0.141
4.229AspAsp: 4.229 ± 0.417
5.041AspGlu: 5.041 ± 0.447
3.165AspPhe: 3.165 ± 0.376
5.741AspGly: 5.741 ± 0.384
0.896AspHis: 0.896 ± 0.177
3.501AspIle: 3.501 ± 0.356
3.977AspLys: 3.977 ± 0.403
4.733AspLeu: 4.733 ± 0.35
1.876AspMet: 1.876 ± 0.219
3.277AspAsn: 3.277 ± 0.345
2.24AspPro: 2.24 ± 0.281
1.54AspGln: 1.54 ± 0.205
2.913AspArg: 2.913 ± 0.227
4.033AspSer: 4.033 ± 0.412
3.725AspThr: 3.725 ± 0.394
4.677AspVal: 4.677 ± 0.464
1.4AspTrp: 1.4 ± 0.185
2.885AspTyr: 2.885 ± 0.291
0.0AspXaa: 0.0 ± 0.0
Glu
5.629GluAla: 5.629 ± 0.504
0.672GluCys: 0.672 ± 0.152
5.349GluAsp: 5.349 ± 0.498
5.433GluGlu: 5.433 ± 0.543
3.529GluPhe: 3.529 ± 0.334
4.425GluGly: 4.425 ± 0.34
1.204GluHis: 1.204 ± 0.2
4.145GluIle: 4.145 ± 0.457
4.537GluLys: 4.537 ± 0.518
5.629GluLeu: 5.629 ± 0.407
2.268GluMet: 2.268 ± 0.27
2.969GluAsn: 2.969 ± 0.259
2.016GluPro: 2.016 ± 0.266
2.436GluGln: 2.436 ± 0.321
4.369GluArg: 4.369 ± 0.395
3.753GluSer: 3.753 ± 0.34
3.893GluThr: 3.893 ± 0.457
4.817GluVal: 4.817 ± 0.414
1.568GluTrp: 1.568 ± 0.263
3.109GluTyr: 3.109 ± 0.318
0.0GluXaa: 0.0 ± 0.0
Phe
2.492PheAla: 2.492 ± 0.338
0.364PheCys: 0.364 ± 0.098
3.249PheAsp: 3.249 ± 0.297
3.333PheGlu: 3.333 ± 0.332
1.624PhePhe: 1.624 ± 0.191
3.165PheGly: 3.165 ± 0.258
0.924PheHis: 0.924 ± 0.191
2.072PheIle: 2.072 ± 0.223
2.156PheLys: 2.156 ± 0.235
3.137PheLeu: 3.137 ± 0.369
0.924PheMet: 0.924 ± 0.15
1.736PheAsn: 1.736 ± 0.221
1.4PhePro: 1.4 ± 0.193
1.316PheGln: 1.316 ± 0.2
2.072PheArg: 2.072 ± 0.243
2.576PheSer: 2.576 ± 0.25
2.38PheThr: 2.38 ± 0.288
3.081PheVal: 3.081 ± 0.329
0.7PheTrp: 0.7 ± 0.168
1.428PheTyr: 1.428 ± 0.218
0.0PheXaa: 0.0 ± 0.0
Gly
4.873GlyAla: 4.873 ± 0.377
0.784GlyCys: 0.784 ± 0.17
4.649GlyAsp: 4.649 ± 0.262
4.621GlyGlu: 4.621 ± 0.283
3.165GlyPhe: 3.165 ± 0.299
5.209GlyGly: 5.209 ± 0.396
1.932GlyHis: 1.932 ± 0.252
4.453GlyIle: 4.453 ± 0.305
4.705GlyLys: 4.705 ± 0.441
5.629GlyLeu: 5.629 ± 0.453
2.492GlyMet: 2.492 ± 0.268
3.893GlyAsn: 3.893 ± 0.351
2.38GlyPro: 2.38 ± 0.294
1.764GlyGln: 1.764 ± 0.284
4.229GlyArg: 4.229 ± 0.45
4.257GlySer: 4.257 ± 0.487
5.153GlyThr: 5.153 ± 0.676
5.181GlyVal: 5.181 ± 0.402
1.596GlyTrp: 1.596 ± 0.2
3.697GlyTyr: 3.697 ± 0.37
0.0GlyXaa: 0.0 ± 0.0
His
1.54HisAla: 1.54 ± 0.214
0.224HisCys: 0.224 ± 0.083
1.26HisAsp: 1.26 ± 0.223
1.148HisGlu: 1.148 ± 0.2
1.036HisPhe: 1.036 ± 0.216
1.512HisGly: 1.512 ± 0.24
0.364HisHis: 0.364 ± 0.094
0.924HisIle: 0.924 ± 0.146
1.12HisLys: 1.12 ± 0.181
1.372HisLeu: 1.372 ± 0.207
0.476HisMet: 0.476 ± 0.124
1.092HisAsn: 1.092 ± 0.219
0.728HisPro: 0.728 ± 0.138
0.532HisGln: 0.532 ± 0.131
1.344HisArg: 1.344 ± 0.198
1.036HisSer: 1.036 ± 0.153
0.84HisThr: 0.84 ± 0.147
1.54HisVal: 1.54 ± 0.204
0.476HisTrp: 0.476 ± 0.105
0.952HisTyr: 0.952 ± 0.189
0.0HisXaa: 0.0 ± 0.0
Ile
4.285IleAla: 4.285 ± 0.389
0.756IleCys: 0.756 ± 0.149
4.033IleAsp: 4.033 ± 0.374
4.061IleGlu: 4.061 ± 0.439
1.12IlePhe: 1.12 ± 0.148
4.117IleGly: 4.117 ± 0.381
0.924IleHis: 0.924 ± 0.16
2.772IleIle: 2.772 ± 0.301
3.305IleLys: 3.305 ± 0.288
3.641IleLeu: 3.641 ± 0.33
1.344IleMet: 1.344 ± 0.175
2.212IleAsn: 2.212 ± 0.225
2.352IlePro: 2.352 ± 0.196
1.988IleGln: 1.988 ± 0.209
3.081IleArg: 3.081 ± 0.285
3.137IleSer: 3.137 ± 0.317
3.389IleThr: 3.389 ± 0.343
3.809IleVal: 3.809 ± 0.358
0.784IleTrp: 0.784 ± 0.163
1.568IleTyr: 1.568 ± 0.235
0.0IleXaa: 0.0 ± 0.0
Lys
4.901LysAla: 4.901 ± 0.506
0.812LysCys: 0.812 ± 0.162
3.165LysAsp: 3.165 ± 0.273
4.285LysGlu: 4.285 ± 0.495
2.212LysPhe: 2.212 ± 0.27
4.005LysGly: 4.005 ± 0.354
1.4LysHis: 1.4 ± 0.199
2.744LysIle: 2.744 ± 0.257
4.341LysLys: 4.341 ± 0.452
3.697LysLeu: 3.697 ± 0.366
1.876LysMet: 1.876 ± 0.267
3.165LysAsn: 3.165 ± 0.346
2.604LysPro: 2.604 ± 0.244
1.904LysGln: 1.904 ± 0.239
4.201LysArg: 4.201 ± 0.405
3.333LysSer: 3.333 ± 0.312
4.201LysThr: 4.201 ± 0.299
4.901LysVal: 4.901 ± 0.4
1.316LysTrp: 1.316 ± 0.194
2.857LysTyr: 2.857 ± 0.311
0.0LysXaa: 0.0 ± 0.0
Leu
5.741LeuAla: 5.741 ± 0.402
0.784LeuCys: 0.784 ± 0.176
5.629LeuAsp: 5.629 ± 0.397
5.321LeuGlu: 5.321 ± 0.452
2.8LeuPhe: 2.8 ± 0.286
5.097LeuGly: 5.097 ± 0.399
1.148LeuHis: 1.148 ± 0.182
3.837LeuIle: 3.837 ± 0.39
3.977LeuLys: 3.977 ± 0.367
4.313LeuLeu: 4.313 ± 0.353
1.904LeuMet: 1.904 ± 0.25
3.025LeuAsn: 3.025 ± 0.291
2.941LeuPro: 2.941 ± 0.311
2.408LeuGln: 2.408 ± 0.253
3.837LeuArg: 3.837 ± 0.264
4.985LeuSer: 4.985 ± 0.369
3.977LeuThr: 3.977 ± 0.35
5.321LeuVal: 5.321 ± 0.354
1.568LeuTrp: 1.568 ± 0.197
2.857LeuTyr: 2.857 ± 0.27
0.0LeuXaa: 0.0 ± 0.0
Met
2.688MetAla: 2.688 ± 0.365
0.336MetCys: 0.336 ± 0.083
1.68MetAsp: 1.68 ± 0.226
1.456MetGlu: 1.456 ± 0.219
0.952MetPhe: 0.952 ± 0.144
1.904MetGly: 1.904 ± 0.227
0.588MetHis: 0.588 ± 0.136
1.428MetIle: 1.428 ± 0.225
2.016MetLys: 2.016 ± 0.241
2.072MetLeu: 2.072 ± 0.267
1.008MetMet: 1.008 ± 0.209
1.82MetAsn: 1.82 ± 0.258
1.456MetPro: 1.456 ± 0.174
0.728MetGln: 0.728 ± 0.169
1.82MetArg: 1.82 ± 0.208
1.568MetSer: 1.568 ± 0.194
2.016MetThr: 2.016 ± 0.192
2.1MetVal: 2.1 ± 0.254
0.448MetTrp: 0.448 ± 0.109
0.812MetTyr: 0.812 ± 0.167
0.0MetXaa: 0.0 ± 0.0
Asn
3.809AsnAla: 3.809 ± 0.351
0.532AsnCys: 0.532 ± 0.133
2.548AsnAsp: 2.548 ± 0.279
3.053AsnGlu: 3.053 ± 0.326
1.68AsnPhe: 1.68 ± 0.238
3.893AsnGly: 3.893 ± 0.404
1.064AsnHis: 1.064 ± 0.175
2.128AsnIle: 2.128 ± 0.269
2.688AsnLys: 2.688 ± 0.322
3.389AsnLeu: 3.389 ± 0.334
1.26AsnMet: 1.26 ± 0.174
2.072AsnAsn: 2.072 ± 0.337
2.38AsnPro: 2.38 ± 0.267
1.54AsnGln: 1.54 ± 0.187
2.268AsnArg: 2.268 ± 0.227
2.576AsnSer: 2.576 ± 0.265
2.688AsnThr: 2.688 ± 0.335
3.221AsnVal: 3.221 ± 0.294
0.756AsnTrp: 0.756 ± 0.155
2.24AsnTyr: 2.24 ± 0.289
0.0AsnXaa: 0.0 ± 0.0
Pro
2.296ProAla: 2.296 ± 0.278
0.252ProCys: 0.252 ± 0.073
2.969ProAsp: 2.969 ± 0.361
3.445ProGlu: 3.445 ± 0.409
1.68ProPhe: 1.68 ± 0.211
2.548ProGly: 2.548 ± 0.28
0.728ProHis: 0.728 ± 0.15
1.96ProIle: 1.96 ± 0.236
2.324ProLys: 2.324 ± 0.326
2.128ProLeu: 2.128 ± 0.222
0.644ProMet: 0.644 ± 0.134
1.932ProAsn: 1.932 ± 0.24
1.232ProPro: 1.232 ± 0.22
1.176ProGln: 1.176 ± 0.188
1.904ProArg: 1.904 ± 0.229
2.492ProSer: 2.492 ± 0.281
2.716ProThr: 2.716 ± 0.342
3.081ProVal: 3.081 ± 0.305
0.56ProTrp: 0.56 ± 0.136
1.456ProTyr: 1.456 ± 0.243
0.0ProXaa: 0.0 ± 0.0
Gln
2.828GlnAla: 2.828 ± 0.408
0.532GlnCys: 0.532 ± 0.106
1.596GlnAsp: 1.596 ± 0.215
2.212GlnGlu: 2.212 ± 0.267
1.26GlnPhe: 1.26 ± 0.185
1.736GlnGly: 1.736 ± 0.193
0.672GlnHis: 0.672 ± 0.128
1.708GlnIle: 1.708 ± 0.261
2.38GlnLys: 2.38 ± 0.242
2.212GlnLeu: 2.212 ± 0.312
1.036GlnMet: 1.036 ± 0.171
1.4GlnAsn: 1.4 ± 0.241
0.7GlnPro: 0.7 ± 0.109
1.148GlnGln: 1.148 ± 0.159
2.464GlnArg: 2.464 ± 0.426
1.792GlnSer: 1.792 ± 0.221
1.848GlnThr: 1.848 ± 0.199
1.876GlnVal: 1.876 ± 0.229
0.7GlnTrp: 0.7 ± 0.13
1.232GlnTyr: 1.232 ± 0.187
0.0GlnXaa: 0.0 ± 0.0
Arg
4.705ArgAla: 4.705 ± 0.358
0.392ArgCys: 0.392 ± 0.097
3.025ArgAsp: 3.025 ± 0.326
4.089ArgGlu: 4.089 ± 0.444
2.464ArgPhe: 2.464 ± 0.278
3.669ArgGly: 3.669 ± 0.36
1.316ArgHis: 1.316 ± 0.145
3.557ArgIle: 3.557 ± 0.254
4.257ArgLys: 4.257 ± 0.471
3.977ArgLeu: 3.977 ± 0.294
1.82ArgMet: 1.82 ± 0.248
2.576ArgAsn: 2.576 ± 0.292
2.24ArgPro: 2.24 ± 0.249
1.708ArgGln: 1.708 ± 0.267
4.229ArgArg: 4.229 ± 0.452
2.408ArgSer: 2.408 ± 0.203
2.52ArgThr: 2.52 ± 0.271
3.809ArgVal: 3.809 ± 0.292
0.98ArgTrp: 0.98 ± 0.199
2.66ArgTyr: 2.66 ± 0.246
0.0ArgXaa: 0.0 ± 0.0
Ser
4.565SerAla: 4.565 ± 0.584
0.56SerCys: 0.56 ± 0.131
3.613SerAsp: 3.613 ± 0.296
4.117SerGlu: 4.117 ± 0.344
2.688SerPhe: 2.688 ± 0.292
5.629SerGly: 5.629 ± 0.73
1.064SerHis: 1.064 ± 0.189
2.828SerIle: 2.828 ± 0.369
3.473SerLys: 3.473 ± 0.325
4.229SerLeu: 4.229 ± 0.354
1.82SerMet: 1.82 ± 0.323
2.324SerAsn: 2.324 ± 0.256
2.128SerPro: 2.128 ± 0.267
2.044SerGln: 2.044 ± 0.216
3.081SerArg: 3.081 ± 0.259
4.761SerSer: 4.761 ± 0.533
3.333SerThr: 3.333 ± 0.419
3.977SerVal: 3.977 ± 0.362
1.428SerTrp: 1.428 ± 0.257
2.1SerTyr: 2.1 ± 0.234
0.0SerXaa: 0.0 ± 0.0
Thr
4.789ThrAla: 4.789 ± 0.648
0.336ThrCys: 0.336 ± 0.099
3.753ThrAsp: 3.753 ± 0.355
3.921ThrGlu: 3.921 ± 0.436
2.576ThrPhe: 2.576 ± 0.305
5.657ThrGly: 5.657 ± 0.664
1.008ThrHis: 1.008 ± 0.193
4.005ThrIle: 4.005 ± 0.381
2.913ThrLys: 2.913 ± 0.287
4.817ThrLeu: 4.817 ± 0.335
1.092ThrMet: 1.092 ± 0.18
2.464ThrAsn: 2.464 ± 0.404
2.716ThrPro: 2.716 ± 0.284
1.848ThrGln: 1.848 ± 0.276
2.744ThrArg: 2.744 ± 0.296
3.865ThrSer: 3.865 ± 0.606
4.173ThrThr: 4.173 ± 0.493
5.153ThrVal: 5.153 ± 0.48
1.008ThrTrp: 1.008 ± 0.199
2.324ThrTyr: 2.324 ± 0.287
0.0ThrXaa: 0.0 ± 0.0
Val
5.461ValAla: 5.461 ± 0.402
0.924ValCys: 0.924 ± 0.166
5.405ValAsp: 5.405 ± 0.417
5.097ValGlu: 5.097 ± 0.52
2.632ValPhe: 2.632 ± 0.262
4.509ValGly: 4.509 ± 0.325
1.344ValHis: 1.344 ± 0.206
3.417ValIle: 3.417 ± 0.377
5.069ValLys: 5.069 ± 0.281
4.621ValLeu: 4.621 ± 0.384
1.988ValMet: 1.988 ± 0.228
3.025ValAsn: 3.025 ± 0.3
2.913ValPro: 2.913 ± 0.318
1.876ValGln: 1.876 ± 0.209
3.865ValArg: 3.865 ± 0.309
4.733ValSer: 4.733 ± 0.445
4.425ValThr: 4.425 ± 0.408
5.321ValVal: 5.321 ± 0.53
1.372ValTrp: 1.372 ± 0.218
3.361ValTyr: 3.361 ± 0.276
0.0ValXaa: 0.0 ± 0.0
Trp
1.344TrpAla: 1.344 ± 0.158
0.224TrpCys: 0.224 ± 0.089
1.596TrpAsp: 1.596 ± 0.252
1.54TrpGlu: 1.54 ± 0.269
0.84TrpPhe: 0.84 ± 0.154
1.568TrpGly: 1.568 ± 0.247
0.532TrpHis: 0.532 ± 0.13
0.56TrpIle: 0.56 ± 0.142
1.036TrpLys: 1.036 ± 0.156
1.568TrpLeu: 1.568 ± 0.271
0.7TrpMet: 0.7 ± 0.157
1.008TrpAsn: 1.008 ± 0.174
0.392TrpPro: 0.392 ± 0.108
0.56TrpGln: 0.56 ± 0.127
0.952TrpArg: 0.952 ± 0.205
1.12TrpSer: 1.12 ± 0.154
1.54TrpThr: 1.54 ± 0.276
1.456TrpVal: 1.456 ± 0.21
0.448TrpTrp: 0.448 ± 0.109
0.784TrpTyr: 0.784 ± 0.155
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.716TyrAla: 2.716 ± 0.298
0.504TyrCys: 0.504 ± 0.113
2.716TyrAsp: 2.716 ± 0.32
3.137TyrGlu: 3.137 ± 0.347
1.176TyrPhe: 1.176 ± 0.196
3.305TyrGly: 3.305 ± 0.244
0.7TyrHis: 0.7 ± 0.138
2.128TyrIle: 2.128 ± 0.221
2.016TyrLys: 2.016 ± 0.25
2.66TyrLeu: 2.66 ± 0.285
1.428TyrMet: 1.428 ± 0.199
2.212TyrAsn: 2.212 ± 0.203
1.68TyrPro: 1.68 ± 0.226
1.288TyrGln: 1.288 ± 0.205
2.436TyrArg: 2.436 ± 0.275
2.688TyrSer: 2.688 ± 0.298
2.857TyrThr: 2.857 ± 0.286
2.828TyrVal: 2.828 ± 0.291
0.84TyrTrp: 0.84 ± 0.124
1.988TyrTyr: 1.988 ± 0.273
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 227 proteins (35709 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski