Streptococcus satellite phage Javan748

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZWA9|A0A4D5ZWA9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan748 OX=2558842 GN=JavanS748_0015 PE=4 SV=1
MM1 pKa = 7.44EE2 pKa = 5.49NEE4 pKa = 4.24FKK6 pKa = 10.57TVTNAKK12 pKa = 9.82GLEE15 pKa = 3.95IPKK18 pKa = 9.98YY19 pKa = 9.85PKK21 pKa = 10.21DD22 pKa = 3.7FKK24 pKa = 11.32KK25 pKa = 10.49LVEE28 pKa = 4.23MDD30 pKa = 3.53RR31 pKa = 11.84QLAEE35 pKa = 4.31YY36 pKa = 10.91LCMNYY41 pKa = 10.23EE42 pKa = 4.02NLDD45 pKa = 3.83NEE47 pKa = 4.47DD48 pKa = 3.65LGAFLEE54 pKa = 4.61TVEE57 pKa = 5.78QGFSWILDD65 pKa = 4.13LIGSKK70 pKa = 10.81DD71 pKa = 3.94LLYY74 pKa = 10.67KK75 pKa = 10.37PKK77 pKa = 10.55SGSNHH82 pKa = 6.36AKK84 pKa = 10.23RR85 pKa = 11.84KK86 pKa = 8.92

Molecular weight: 9.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZXV6|A0A4D5ZXV6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan748 OX=2558842 GN=JavanS748_0003 PE=4 SV=1
MM1 pKa = 7.3NPKK4 pKa = 9.96KK5 pKa = 10.63LSFLLYY11 pKa = 10.21RR12 pKa = 11.84EE13 pKa = 3.97MRR15 pKa = 11.84FISSLGEE22 pKa = 3.68KK23 pKa = 10.38LLATRR28 pKa = 11.84ILFTVTIANTQQ39 pKa = 3.0

Molecular weight: 4.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

2641

39

484

176.1

20.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.377 ± 0.56

0.492 ± 0.103

5.301 ± 0.666

9.012 ± 0.704

4.657 ± 0.663

4.657 ± 0.311

1.931 ± 0.237

6.702 ± 0.629

10.564 ± 0.546

9.807 ± 0.835

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.575 ± 0.305

5.187 ± 0.314

2.651 ± 0.233

4.354 ± 0.611

5.377 ± 0.542

5.452 ± 0.476

5.301 ± 0.575

4.922 ± 0.421

0.909 ± 0.103

4.771 ± 0.386
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski