Roseburia sp. CAG:303

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia; environmental samples

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2401 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7IQT2|R7IQT2_9FIRM Aspartokinase OS=Roseburia sp. CAG:303 OX=1262944 GN=BN596_01988 PE=3 SV=1
MM1 pKa = 7.39NKK3 pKa = 9.58RR4 pKa = 11.84MRR6 pKa = 11.84NMLVLSGVCVLCFAGYY22 pKa = 9.58GISVAMQKK30 pKa = 8.9DD31 pKa = 3.82TSQEE35 pKa = 3.92EE36 pKa = 4.32EE37 pKa = 4.11KK38 pKa = 10.74SEE40 pKa = 4.04KK41 pKa = 10.6LFAVSADD48 pKa = 4.46DD49 pKa = 3.5ITQITYY55 pKa = 9.09NWQEE59 pKa = 3.78EE60 pKa = 4.48EE61 pKa = 3.87IALEE65 pKa = 4.09KK66 pKa = 11.01DD67 pKa = 3.53GNDD70 pKa = 3.15WQYY73 pKa = 11.89KK74 pKa = 8.39EE75 pKa = 5.19DD76 pKa = 3.87AAFSLNQTMAEE87 pKa = 4.21TMSEE91 pKa = 4.07ALSGAEE97 pKa = 3.65VLQKK101 pKa = 10.19IEE103 pKa = 4.41EE104 pKa = 4.31KK105 pKa = 10.88DD106 pKa = 3.17VDD108 pKa = 4.07LQSFGLEE115 pKa = 4.16TPALAVTFSDD125 pKa = 4.97GDD127 pKa = 3.77GQQYY131 pKa = 10.18QLSFGNYY138 pKa = 5.83NTAAKK143 pKa = 10.13GYY145 pKa = 9.36YY146 pKa = 10.09AMKK149 pKa = 10.64NDD151 pKa = 4.27DD152 pKa = 3.83GCVYY156 pKa = 10.01MVDD159 pKa = 4.1SEE161 pKa = 4.67TMTDD165 pKa = 4.53FEE167 pKa = 4.73YY168 pKa = 11.31GLYY171 pKa = 10.57DD172 pKa = 4.81LLVLDD177 pKa = 5.97EE178 pKa = 4.99IPSVDD183 pKa = 3.46ADD185 pKa = 3.93YY186 pKa = 11.67VSGITISDD194 pKa = 3.46SGKK197 pKa = 6.65TTEE200 pKa = 3.78YY201 pKa = 9.4TYY203 pKa = 10.58QIEE206 pKa = 4.35EE207 pKa = 4.25VQEE210 pKa = 3.94EE211 pKa = 4.7TTQEE215 pKa = 3.93EE216 pKa = 4.79TSASQVVWYY225 pKa = 9.82AWDD228 pKa = 3.6GTDD231 pKa = 5.51KK232 pKa = 10.81VRR234 pKa = 11.84CDD236 pKa = 3.19STEE239 pKa = 3.8FAEE242 pKa = 4.66LTDD245 pKa = 4.81EE246 pKa = 4.06ILKK249 pKa = 10.47ISASNAADD257 pKa = 3.64YY258 pKa = 11.14NAGSAEE264 pKa = 4.29KK265 pKa = 10.57LSEE268 pKa = 4.16TYY270 pKa = 10.81GIADD274 pKa = 3.65RR275 pKa = 11.84MITVNYY281 pKa = 9.8VDD283 pKa = 5.29ADD285 pKa = 3.62TDD287 pKa = 3.59EE288 pKa = 4.93DD289 pKa = 3.84KK290 pKa = 11.3SYY292 pKa = 9.23TLHH295 pKa = 6.72FGSSDD300 pKa = 3.4GAGNVYY306 pKa = 7.99MTVDD310 pKa = 4.59DD311 pKa = 4.3SMQVQLVEE319 pKa = 4.12EE320 pKa = 4.33SLVNSIFGEE329 pKa = 4.65GNN331 pKa = 2.94

Molecular weight: 36.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7IWL2|R7IWL2_9FIRM DNA-binding response regulator OS=Roseburia sp. CAG:303 OX=1262944 GN=BN596_00578 PE=4 SV=1
MM1 pKa = 7.3RR2 pKa = 11.84QTMGVTYY9 pKa = 10.27NPKK12 pKa = 9.4KK13 pKa = 9.16RR14 pKa = 11.84ARR16 pKa = 11.84AKK18 pKa = 9.24VHH20 pKa = 5.63GFRR23 pKa = 11.84ARR25 pKa = 11.84MSTANGRR32 pKa = 11.84KK33 pKa = 8.89VLAARR38 pKa = 11.84RR39 pKa = 11.84AKK41 pKa = 9.63GRR43 pKa = 11.84KK44 pKa = 9.18RR45 pKa = 11.84ISAA48 pKa = 3.72

Molecular weight: 5.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2401

0

2401

776360

30

2484

323.3

36.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.147 ± 0.051

1.445 ± 0.022

5.787 ± 0.041

8.076 ± 0.061

4.007 ± 0.035

6.819 ± 0.044

1.638 ± 0.023

7.773 ± 0.044

7.279 ± 0.045

8.485 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.164 ± 0.026

4.729 ± 0.039

2.849 ± 0.029

3.252 ± 0.031

4.076 ± 0.037

5.841 ± 0.044

5.515 ± 0.049

6.84 ± 0.041

0.801 ± 0.019

4.455 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski