Amino acid dipepetide frequency for Roseburia sp. CAG:303

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.85AlaAla: 6.85 ± 0.11	0.983AlaCys: 0.983 ± 0.035	4.7AlaAsp: 4.7 ± 0.078	5.749AlaGlu: 5.749 ± 0.101	2.785AlaPhe: 2.785 ± 0.071	6.293AlaGly: 6.293 ± 0.098	0.909AlaHis: 0.909 ± 0.035	4.691AlaIle: 4.691 ± 0.083	4.806AlaLys: 4.806 ± 0.091	6.064AlaLeu: 6.064 ± 0.102	2.222AlaMet: 2.222 ± 0.053	2.577AlaAsn: 2.577 ± 0.061	1.557AlaPro: 1.557 ± 0.053	2.261AlaGln: 2.261 ± 0.057	2.513AlaArg: 2.513 ± 0.054	3.68AlaSer: 3.68 ± 0.075	2.901AlaThr: 2.901 ± 0.074	6.529AlaVal: 6.529 ± 0.099	0.578AlaTrp: 0.578 ± 0.027	2.805AlaTyr: 2.805 ± 0.061	0.014AlaXaa: 0.014 ± 0.005
Cys	0.885CysAla: 0.885 ± 0.038	0.249CysCys: 0.249 ± 0.019	0.777CysAsp: 0.777 ± 0.037	0.974CysGlu: 0.974 ± 0.037	0.589CysPhe: 0.589 ± 0.029	1.465CysGly: 1.465 ± 0.049	0.337CysHis: 0.337 ± 0.022	1.255CysIle: 1.255 ± 0.037	0.806CysLys: 0.806 ± 0.029	1.1CysLeu: 1.1 ± 0.04	0.474CysMet: 0.474 ± 0.023	0.587CysAsn: 0.587 ± 0.03	0.549CysPro: 0.549 ± 0.029	0.375CysGln: 0.375 ± 0.02	0.728CysArg: 0.728 ± 0.033	0.944CysSer: 0.944 ± 0.041	0.755CysThr: 0.755 ± 0.036	0.921CysVal: 0.921 ± 0.035	0.111CysTrp: 0.111 ± 0.012	0.569CysTyr: 0.569 ± 0.03	0.0CysXaa: 0.0 ± 0.0
Asp	4.144AspAla: 4.144 ± 0.08	0.831AspCys: 0.831 ± 0.035	3.0AspAsp: 3.0 ± 0.079	4.972AspGlu: 4.972 ± 0.094	2.669AspPhe: 2.669 ± 0.07	4.506AspGly: 4.506 ± 0.105	0.741AspHis: 0.741 ± 0.033	5.136AspIle: 5.136 ± 0.085	4.014AspLys: 4.014 ± 0.07	4.374AspLeu: 4.374 ± 0.077	2.022AspMet: 2.022 ± 0.054	2.531AspAsn: 2.531 ± 0.068	1.365AspPro: 1.365 ± 0.045	1.155AspGln: 1.155 ± 0.039	2.389AspArg: 2.389 ± 0.061	3.382AspSer: 3.382 ± 0.065	3.294AspThr: 3.294 ± 0.074	3.898AspVal: 3.898 ± 0.081	0.471AspTrp: 0.471 ± 0.027	2.975AspTyr: 2.975 ± 0.064	0.001AspXaa: 0.001 ± 0.001
Glu	5.038GluAla: 5.038 ± 0.097	0.906GluCys: 0.906 ± 0.036	4.56GluAsp: 4.56 ± 0.086	8.429GluGlu: 8.429 ± 0.163	2.633GluPhe: 2.633 ± 0.055	4.172GluGly: 4.172 ± 0.083	1.401GluHis: 1.401 ± 0.044	6.239GluIle: 6.239 ± 0.096	7.955GluLys: 7.955 ± 0.115	7.012GluLeu: 7.012 ± 0.116	2.549GluMet: 2.549 ± 0.063	5.067GluAsn: 5.067 ± 0.079	1.708GluPro: 1.708 ± 0.054	3.13GluGln: 3.13 ± 0.074	3.042GluArg: 3.042 ± 0.069	3.711GluSer: 3.711 ± 0.071	4.231GluThr: 4.231 ± 0.089	4.668GluVal: 4.668 ± 0.089	0.634GluTrp: 0.634 ± 0.032	3.672GluTyr: 3.672 ± 0.077	0.005GluXaa: 0.005 ± 0.003
Phe	2.964PheAla: 2.964 ± 0.071	0.787PheCys: 0.787 ± 0.031	2.489PheAsp: 2.489 ± 0.053	2.835PheGlu: 2.835 ± 0.069	1.796PhePhe: 1.796 ± 0.05	2.858PheGly: 2.858 ± 0.072	0.844PheHis: 0.844 ± 0.036	2.811PheIle: 2.811 ± 0.065	2.067PheLys: 2.067 ± 0.05	3.836PheLeu: 3.836 ± 0.09	1.132PheMet: 1.132 ± 0.038	1.427PheAsn: 1.427 ± 0.043	1.182PhePro: 1.182 ± 0.038	1.202PheGln: 1.202 ± 0.041	1.676PheArg: 1.676 ± 0.048	2.814PheSer: 2.814 ± 0.065	2.384PheThr: 2.384 ± 0.056	2.813PheVal: 2.813 ± 0.062	0.393PheTrp: 0.393 ± 0.026	1.75PheTyr: 1.75 ± 0.051	0.006PheXaa: 0.006 ± 0.002
Gly	4.226GlyAla: 4.226 ± 0.08	1.166GlyCys: 1.166 ± 0.052	3.516GlyAsp: 3.516 ± 0.075	4.698GlyGlu: 4.698 ± 0.082	3.021GlyPhe: 3.021 ± 0.064	4.113GlyGly: 4.113 ± 0.096	1.135GlyHis: 1.135 ± 0.033	6.519GlyIle: 6.519 ± 0.09	5.906GlyLys: 5.906 ± 0.093	5.315GlyLeu: 5.315 ± 0.082	2.451GlyMet: 2.451 ± 0.058	3.519GlyAsn: 3.519 ± 0.088	1.136GlyPro: 1.136 ± 0.041	2.083GlyGln: 2.083 ± 0.057	2.805GlyArg: 2.805 ± 0.069	3.947GlySer: 3.947 ± 0.085	4.175GlyThr: 4.175 ± 0.079	4.355GlyVal: 4.355 ± 0.083	0.571GlyTrp: 0.571 ± 0.026	3.529GlyTyr: 3.529 ± 0.069	0.01GlyXaa: 0.01 ± 0.004
His	0.996HisAla: 0.996 ± 0.039	0.298HisCys: 0.298 ± 0.022	0.848HisAsp: 0.848 ± 0.036	1.051HisGlu: 1.051 ± 0.035	0.791HisPhe: 0.791 ± 0.03	1.234HisGly: 1.234 ± 0.039	0.344HisHis: 0.344 ± 0.04	1.516HisIle: 1.516 ± 0.042	0.958HisLys: 0.958 ± 0.034	1.323HisLeu: 1.323 ± 0.046	0.551HisMet: 0.551 ± 0.029	0.755HisAsn: 0.755 ± 0.029	0.772HisPro: 0.772 ± 0.033	0.444HisGln: 0.444 ± 0.023	0.751HisArg: 0.751 ± 0.033	0.915HisSer: 0.915 ± 0.034	0.954HisThr: 0.954 ± 0.037	0.96HisVal: 0.96 ± 0.034	0.138HisTrp: 0.138 ± 0.012	0.778HisTyr: 0.778 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.831IleAla: 5.831 ± 0.1	1.389IleCys: 1.389 ± 0.041	4.23IleAsp: 4.23 ± 0.068	5.137IleGlu: 5.137 ± 0.088	3.127IlePhe: 3.127 ± 0.071	4.846IleGly: 4.846 ± 0.093	1.392IleHis: 1.392 ± 0.039	5.893IleIle: 5.893 ± 0.122	4.494IleLys: 4.494 ± 0.086	7.372IleLeu: 7.372 ± 0.126	2.228IleMet: 2.228 ± 0.054	3.255IleAsn: 3.255 ± 0.062	2.914IlePro: 2.914 ± 0.063	2.494IleGln: 2.494 ± 0.055	3.948IleArg: 3.948 ± 0.074	5.47IleSer: 5.47 ± 0.096	4.701IleThr: 4.701 ± 0.082	5.206IleVal: 5.206 ± 0.09	0.594IleTrp: 0.594 ± 0.022	3.21IleTyr: 3.21 ± 0.07	0.004IleXaa: 0.004 ± 0.002
Lys	4.736LysAla: 4.736 ± 0.092	0.824LysCys: 0.824 ± 0.032	4.239LysAsp: 4.239 ± 0.088	7.32LysGlu: 7.32 ± 0.115	2.088LysPhe: 2.088 ± 0.052	3.909LysGly: 3.909 ± 0.072	1.041LysHis: 1.041 ± 0.035	5.599LysIle: 5.599 ± 0.101	7.007LysLys: 7.007 ± 0.125	5.749LysLeu: 5.749 ± 0.099	2.505LysMet: 2.505 ± 0.055	4.341LysAsn: 4.341 ± 0.082	1.837LysPro: 1.837 ± 0.05	2.49LysGln: 2.49 ± 0.061	2.898LysArg: 2.898 ± 0.065	3.671LysSer: 3.671 ± 0.071	3.952LysThr: 3.952 ± 0.071	4.596LysVal: 4.596 ± 0.09	0.562LysTrp: 0.562 ± 0.028	3.421LysTyr: 3.421 ± 0.07	0.005LysXaa: 0.005 ± 0.003
Leu	6.319LeuAla: 6.319 ± 0.086	1.351LeuCys: 1.351 ± 0.047	4.887LeuAsp: 4.887 ± 0.079	6.219LeuGlu: 6.219 ± 0.121	3.723LeuPhe: 3.723 ± 0.074	5.358LeuGly: 5.358 ± 0.084	1.439LeuHis: 1.439 ± 0.048	5.965LeuIle: 5.965 ± 0.11	6.406LeuLys: 6.406 ± 0.107	7.664LeuLeu: 7.664 ± 0.125	2.53LeuMet: 2.53 ± 0.058	4.144LeuAsn: 4.144 ± 0.082	2.957LeuPro: 2.957 ± 0.071	2.717LeuGln: 2.717 ± 0.062	3.206LeuArg: 3.206 ± 0.075	5.84LeuSer: 5.84 ± 0.082	4.804LeuThr: 4.804 ± 0.093	5.197LeuVal: 5.197 ± 0.09	0.663LeuTrp: 0.663 ± 0.031	3.457LeuTyr: 3.457 ± 0.081	0.008LeuXaa: 0.008 ± 0.003
Met	2.478MetAla: 2.478 ± 0.051	0.379MetCys: 0.379 ± 0.023	2.006MetAsp: 2.006 ± 0.053	2.666MetGlu: 2.666 ± 0.066	1.076MetPhe: 1.076 ± 0.042	2.061MetGly: 2.061 ± 0.057	0.495MetHis: 0.495 ± 0.023	2.291MetIle: 2.291 ± 0.053	2.575MetLys: 2.575 ± 0.053	2.583MetLeu: 2.583 ± 0.066	0.929MetMet: 0.929 ± 0.038	1.826MetAsn: 1.826 ± 0.054	1.027MetPro: 1.027 ± 0.036	1.132MetGln: 1.132 ± 0.04	1.258MetArg: 1.258 ± 0.048	1.79MetSer: 1.79 ± 0.045	1.878MetThr: 1.878 ± 0.053	1.939MetVal: 1.939 ± 0.047	0.182MetTrp: 0.182 ± 0.016	1.068MetTyr: 1.068 ± 0.037	0.001MetXaa: 0.001 ± 0.001
Asn	3.279AsnAla: 3.279 ± 0.069	0.679AsnCys: 0.679 ± 0.029	2.398AsnAsp: 2.398 ± 0.061	3.214AsnGlu: 3.214 ± 0.065	1.642AsnPhe: 1.642 ± 0.046	3.634AsnGly: 3.634 ± 0.076	0.895AsnHis: 0.895 ± 0.035	4.16AsnIle: 4.16 ± 0.085	2.916AsnLys: 2.916 ± 0.072	3.872AsnLeu: 3.872 ± 0.067	1.566AsnMet: 1.566 ± 0.045	2.197AsnAsn: 2.197 ± 0.076	1.926AsnPro: 1.926 ± 0.052	1.629AsnGln: 1.629 ± 0.047	2.127AsnArg: 2.127 ± 0.054	2.624AsnSer: 2.624 ± 0.062	2.709AsnThr: 2.709 ± 0.067	3.203AsnVal: 3.203 ± 0.067	0.425AsnTrp: 0.425 ± 0.026	2.187AsnTyr: 2.187 ± 0.065	0.004AsnXaa: 0.004 ± 0.002
Pro	2.249ProAla: 2.249 ± 0.056	0.371ProCys: 0.371 ± 0.023	2.249ProAsp: 2.249 ± 0.058	3.196ProGlu: 3.196 ± 0.075	1.269ProPhe: 1.269 ± 0.042	2.06ProGly: 2.06 ± 0.058	0.459ProHis: 0.459 ± 0.024	1.512ProIle: 1.512 ± 0.043	1.584ProLys: 1.584 ± 0.056	2.263ProLeu: 2.263 ± 0.058	0.797ProMet: 0.797 ± 0.037	0.983ProAsn: 0.983 ± 0.037	0.571ProPro: 0.571 ± 0.03	1.045ProGln: 1.045 ± 0.039	0.752ProArg: 0.752 ± 0.032	1.412ProSer: 1.412 ± 0.051	1.143ProThr: 1.143 ± 0.04	3.022ProVal: 3.022 ± 0.074	0.252ProTrp: 0.252 ± 0.02	1.296ProTyr: 1.296 ± 0.038	0.005ProXaa: 0.005 ± 0.002
Gln	2.463GlnAla: 2.463 ± 0.067	0.353GlnCys: 0.353 ± 0.021	1.674GlnAsp: 1.674 ± 0.041	2.983GlnGlu: 2.983 ± 0.075	1.177GlnPhe: 1.177 ± 0.032	2.007GlnGly: 2.007 ± 0.055	0.41GlnHis: 0.41 ± 0.022	2.727GlnIle: 2.727 ± 0.063	2.836GlnLys: 2.836 ± 0.062	2.423GlnLeu: 2.423 ± 0.061	1.132GlnMet: 1.132 ± 0.041	1.748GlnAsn: 1.748 ± 0.049	0.939GlnPro: 0.939 ± 0.037	1.159GlnGln: 1.159 ± 0.044	1.18GlnArg: 1.18 ± 0.037	1.73GlnSer: 1.73 ± 0.053	1.851GlnThr: 1.851 ± 0.053	2.109GlnVal: 2.109 ± 0.056	0.256GlnTrp: 0.256 ± 0.019	1.367GlnTyr: 1.367 ± 0.043	0.001GlnXaa: 0.001 ± 0.001
Arg	2.289ArgAla: 2.289 ± 0.057	0.495ArgCys: 0.495 ± 0.027	2.027ArgAsp: 2.027 ± 0.055	3.838ArgGlu: 3.838 ± 0.085	1.624ArgPhe: 1.624 ± 0.048	2.206ArgGly: 2.206 ± 0.054	0.703ArgHis: 0.703 ± 0.03	3.61ArgIle: 3.61 ± 0.079	3.799ArgLys: 3.799 ± 0.072	3.335ArgLeu: 3.335 ± 0.064	1.556ArgMet: 1.556 ± 0.045	2.145ArgAsn: 2.145 ± 0.05	1.042ArgPro: 1.042 ± 0.036	1.556ArgGln: 1.556 ± 0.046	1.915ArgArg: 1.915 ± 0.052	1.837ArgSer: 1.837 ± 0.047	2.205ArgThr: 2.205 ± 0.06	2.4ArgVal: 2.4 ± 0.053	0.292ArgTrp: 0.292 ± 0.02	1.883ArgTyr: 1.883 ± 0.049	0.005ArgXaa: 0.005 ± 0.003
Ser	4.265SerAla: 4.265 ± 0.088	0.791SerCys: 0.791 ± 0.033	3.631SerAsp: 3.631 ± 0.075	4.339SerGlu: 4.339 ± 0.083	2.547SerPhe: 2.547 ± 0.061	5.07SerGly: 5.07 ± 0.086	0.917SerHis: 0.917 ± 0.037	4.208SerIle: 4.208 ± 0.081	3.276SerLys: 3.276 ± 0.067	4.932SerLeu: 4.932 ± 0.081	1.838SerMet: 1.838 ± 0.055	2.303SerAsn: 2.303 ± 0.066	1.462SerPro: 1.462 ± 0.048	1.851SerGln: 1.851 ± 0.051	2.608SerArg: 2.608 ± 0.055	3.823SerSer: 3.823 ± 0.094	2.836SerThr: 2.836 ± 0.076	4.576SerVal: 4.576 ± 0.081	0.479SerTrp: 0.479 ± 0.025	2.656SerTyr: 2.656 ± 0.066	0.006SerXaa: 0.006 ± 0.003
Thr	4.637ThrAla: 4.637 ± 0.092	0.626ThrCys: 0.626 ± 0.03	3.76ThrAsp: 3.76 ± 0.091	4.506ThrGlu: 4.506 ± 0.096	2.12ThrPhe: 2.12 ± 0.055	5.129ThrGly: 5.129 ± 0.088	0.783ThrHis: 0.783 ± 0.03	4.088ThrIle: 4.088 ± 0.083	3.17ThrLys: 3.17 ± 0.075	4.44ThrLeu: 4.44 ± 0.086	1.446ThrMet: 1.446 ± 0.048	2.114ThrAsn: 2.114 ± 0.064	1.77ThrPro: 1.77 ± 0.046	1.676ThrGln: 1.676 ± 0.062	1.825ThrArg: 1.825 ± 0.053	2.977ThrSer: 2.977 ± 0.065	3.033ThrThr: 3.033 ± 0.104	4.291ThrVal: 4.291 ± 0.102	0.426ThrTrp: 0.426 ± 0.024	2.331ThrTyr: 2.331 ± 0.065	0.005ThrXaa: 0.005 ± 0.002
Val	4.426ValAla: 4.426 ± 0.08	1.207ValCys: 1.207 ± 0.035	3.679ValAsp: 3.679 ± 0.076	4.718ValGlu: 4.718 ± 0.076	3.026ValPhe: 3.026 ± 0.06	3.663ValGly: 3.663 ± 0.079	0.991ValHis: 0.991 ± 0.035	5.648ValIle: 5.648 ± 0.095	4.853ValLys: 4.853 ± 0.078	6.622ValLeu: 6.622 ± 0.096	2.15ValMet: 2.15 ± 0.053	3.259ValAsn: 3.259 ± 0.062	2.163ValPro: 2.163 ± 0.054	2.066ValGln: 2.066 ± 0.05	2.696ValArg: 2.696 ± 0.069	4.75ValSer: 4.75 ± 0.09	4.446ValThr: 4.446 ± 0.099	4.498ValVal: 4.498 ± 0.083	0.573ValTrp: 0.573 ± 0.028	2.961ValTyr: 2.961 ± 0.068	0.004ValXaa: 0.004 ± 0.002
Trp	0.475TrpAla: 0.475 ± 0.025	0.117TrpCys: 0.117 ± 0.012	0.422TrpAsp: 0.422 ± 0.026	0.559TrpGlu: 0.559 ± 0.029	0.356TrpPhe: 0.356 ± 0.023	0.567TrpGly: 0.567 ± 0.028	0.162TrpHis: 0.162 ± 0.014	0.627TrpIle: 0.627 ± 0.03	0.759TrpLys: 0.759 ± 0.031	0.717TrpLeu: 0.717 ± 0.03	0.258TrpMet: 0.258 ± 0.019	0.471TrpAsn: 0.471 ± 0.025	0.16TrpPro: 0.16 ± 0.016	0.322TrpGln: 0.322 ± 0.021	0.272TrpArg: 0.272 ± 0.019	0.482TrpSer: 0.482 ± 0.029	0.393TrpThr: 0.393 ± 0.024	0.404TrpVal: 0.404 ± 0.024	0.09TrpTrp: 0.09 ± 0.01	0.392TrpTyr: 0.392 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.914TyrAla: 2.914 ± 0.066	0.648TyrCys: 0.648 ± 0.027	2.776TyrAsp: 2.776 ± 0.06	3.355TyrGlu: 3.355 ± 0.073	2.008TyrPhe: 2.008 ± 0.052	3.031TyrGly: 3.031 ± 0.064	0.974TyrHis: 0.974 ± 0.033	3.233TyrIle: 3.233 ± 0.074	2.603TyrLys: 2.603 ± 0.061	3.849TyrLeu: 3.849 ± 0.074	1.297TyrMet: 1.297 ± 0.046	2.034TyrAsn: 2.034 ± 0.061	1.452TyrPro: 1.452 ± 0.047	1.736TyrGln: 1.736 ± 0.049	2.164TyrArg: 2.164 ± 0.057	2.608TyrSer: 2.608 ± 0.071	2.504TyrThr: 2.504 ± 0.074	2.811TyrVal: 2.811 ± 0.065	0.31TyrTrp: 0.31 ± 0.02	2.24TyrTyr: 2.24 ± 0.067	0.003TyrXaa: 0.003 ± 0.002
Xaa	0.005XaaAla: 0.005 ± 0.002	0.001XaaCys: 0.001 ± 0.001	0.003XaaAsp: 0.003 ± 0.002	0.005XaaGlu: 0.005 ± 0.002	0.003XaaPhe: 0.003 ± 0.002	0.013XaaGly: 0.013 ± 0.004	0.004XaaHis: 0.004 ± 0.002	0.005XaaIle: 0.005 ± 0.002	0.001XaaLys: 0.001 ± 0.001	0.01XaaLeu: 0.01 ± 0.003	0.003XaaMet: 0.003 ± 0.002	0.004XaaAsn: 0.004 ± 0.002	0.001XaaPro: 0.001 ± 0.001	0.003XaaGln: 0.003 ± 0.002	0.009XaaArg: 0.009 ± 0.003	0.008XaaSer: 0.008 ± 0.003	0.004XaaThr: 0.004 ± 0.002	0.004XaaVal: 0.004 ± 0.002	0.004XaaTrp: 0.004 ± 0.002	0.0XaaTyr: 0.0 ± 0.0	0.124XaaXaa: 0.124 ± 0.017

Statistics based on 2401 proteins (776360 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---