Amino acid dipepetide frequency for Microbacterium sediminis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.065AlaAla: 22.065 ± 0.294	0.705AlaCys: 0.705 ± 0.032	8.735AlaAsp: 8.735 ± 0.126	9.139AlaGlu: 9.139 ± 0.138	4.191AlaPhe: 4.191 ± 0.074	12.587AlaGly: 12.587 ± 0.158	2.588AlaHis: 2.588 ± 0.067	6.687AlaIle: 6.687 ± 0.106	2.527AlaLys: 2.527 ± 0.068	15.086AlaLeu: 15.086 ± 0.195	2.968AlaMet: 2.968 ± 0.056	2.135AlaAsn: 2.135 ± 0.059	7.404AlaPro: 7.404 ± 0.146	3.977AlaGln: 3.977 ± 0.077	10.237AlaArg: 10.237 ± 0.149	6.501AlaSer: 6.501 ± 0.102	7.383AlaThr: 7.383 ± 0.114	11.907AlaVal: 11.907 ± 0.15	2.177AlaTrp: 2.177 ± 0.061	2.594AlaTyr: 2.594 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	0.699CysAla: 0.699 ± 0.033	0.046CysCys: 0.046 ± 0.008	0.292CysAsp: 0.292 ± 0.021	0.252CysGlu: 0.252 ± 0.018	0.17CysPhe: 0.17 ± 0.014	0.561CysGly: 0.561 ± 0.029	0.108CysHis: 0.108 ± 0.01	0.162CysIle: 0.162 ± 0.014	0.058CysLys: 0.058 ± 0.008	0.438CysLeu: 0.438 ± 0.022	0.075CysMet: 0.075 ± 0.012	0.092CysAsn: 0.092 ± 0.011	0.248CysPro: 0.248 ± 0.017	0.119CysGln: 0.119 ± 0.014	0.304CysArg: 0.304 ± 0.024	0.263CysSer: 0.263 ± 0.022	0.288CysThr: 0.288 ± 0.022	0.422CysVal: 0.422 ± 0.026	0.069CysTrp: 0.069 ± 0.009	0.099CysTyr: 0.099 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	9.232AspAla: 9.232 ± 0.135	0.218AspCys: 0.218 ± 0.015	4.386AspAsp: 4.386 ± 0.08	4.92AspGlu: 4.92 ± 0.09	1.601AspPhe: 1.601 ± 0.051	6.058AspGly: 6.058 ± 0.104	1.177AspHis: 1.177 ± 0.037	2.487AspIle: 2.487 ± 0.065	0.922AspLys: 0.922 ± 0.038	5.923AspLeu: 5.923 ± 0.102	0.868AspMet: 0.868 ± 0.034	0.893AspAsn: 0.893 ± 0.035	4.543AspPro: 4.543 ± 0.078	1.434AspGln: 1.434 ± 0.043	4.577AspArg: 4.577 ± 0.079	1.998AspSer: 1.998 ± 0.048	2.707AspThr: 2.707 ± 0.061	5.438AspVal: 5.438 ± 0.09	0.847AspTrp: 0.847 ± 0.031	1.259AspTyr: 1.259 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	8.272GluAla: 8.272 ± 0.125	0.235GluCys: 0.235 ± 0.017	2.808GluAsp: 2.808 ± 0.069	3.414GluGlu: 3.414 ± 0.068	1.748GluPhe: 1.748 ± 0.052	4.684GluGly: 4.684 ± 0.085	1.461GluHis: 1.461 ± 0.039	3.013GluIle: 3.013 ± 0.07	1.396GluLys: 1.396 ± 0.051	7.036GluLeu: 7.036 ± 0.102	0.958GluMet: 0.958 ± 0.034	1.244GluAsn: 1.244 ± 0.045	3.632GluPro: 3.632 ± 0.078	2.221GluGln: 2.221 ± 0.056	6.033GluArg: 6.033 ± 0.108	2.621GluSer: 2.621 ± 0.055	3.185GluThr: 3.185 ± 0.065	4.931GluVal: 4.931 ± 0.096	0.938GluTrp: 0.938 ± 0.038	1.222GluTyr: 1.222 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	4.501PheAla: 4.501 ± 0.066	0.147PheCys: 0.147 ± 0.013	2.311PheAsp: 2.311 ± 0.058	1.81PheGlu: 1.81 ± 0.047	0.99PhePhe: 0.99 ± 0.036	3.264PheGly: 3.264 ± 0.065	0.567PheHis: 0.567 ± 0.03	1.07PheIle: 1.07 ± 0.04	0.401PheLys: 0.401 ± 0.024	2.93PheLeu: 2.93 ± 0.065	0.421PheMet: 0.421 ± 0.024	0.628PheAsn: 0.628 ± 0.028	1.385PhePro: 1.385 ± 0.048	0.757PheGln: 0.757 ± 0.03	1.869PheArg: 1.869 ± 0.048	1.483PheSer: 1.483 ± 0.039	1.967PheThr: 1.967 ± 0.056	2.744PheVal: 2.744 ± 0.065	0.44PheTrp: 0.44 ± 0.029	0.634PheTyr: 0.634 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	11.855GlyAla: 11.855 ± 0.154	0.556GlyCys: 0.556 ± 0.028	5.309GlyAsp: 5.309 ± 0.091	5.586GlyGlu: 5.586 ± 0.096	3.244GlyPhe: 3.244 ± 0.075	7.927GlyGly: 7.927 ± 0.137	1.74GlyHis: 1.74 ± 0.049	4.776GlyIle: 4.776 ± 0.095	1.984GlyLys: 1.984 ± 0.06	8.858GlyLeu: 8.858 ± 0.105	1.992GlyMet: 1.992 ± 0.054	1.605GlyAsn: 1.605 ± 0.048	3.93GlyPro: 3.93 ± 0.073	2.512GlyGln: 2.512 ± 0.07	6.622GlyArg: 6.622 ± 0.101	5.055GlySer: 5.055 ± 0.089	5.356GlyThr: 5.356 ± 0.098	8.156GlyVal: 8.156 ± 0.104	1.671GlyTrp: 1.671 ± 0.048	2.088GlyTyr: 2.088 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	2.492HisAla: 2.492 ± 0.06	0.102HisCys: 0.102 ± 0.012	1.297HisAsp: 1.297 ± 0.05	1.177HisGlu: 1.177 ± 0.037	0.483HisPhe: 0.483 ± 0.024	2.015HisGly: 2.015 ± 0.056	0.55HisHis: 0.55 ± 0.031	0.693HisIle: 0.693 ± 0.026	0.265HisLys: 0.265 ± 0.017	1.952HisLeu: 1.952 ± 0.054	0.322HisMet: 0.322 ± 0.023	0.317HisAsn: 0.317 ± 0.018	1.457HisPro: 1.457 ± 0.049	0.454HisGln: 0.454 ± 0.025	1.536HisArg: 1.536 ± 0.049	0.836HisSer: 0.836 ± 0.03	0.992HisThr: 0.992 ± 0.035	1.659HisVal: 1.659 ± 0.047	0.257HisTrp: 0.257 ± 0.017	0.395HisTyr: 0.395 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	7.805IleAla: 7.805 ± 0.113	0.231IleCys: 0.231 ± 0.016	3.731IleAsp: 3.731 ± 0.066	3.217IleGlu: 3.217 ± 0.065	1.081IlePhe: 1.081 ± 0.045	4.889IleGly: 4.889 ± 0.085	0.75IleHis: 0.75 ± 0.029	1.822IleIle: 1.822 ± 0.058	0.729IleLys: 0.729 ± 0.034	3.679IleLeu: 3.679 ± 0.079	0.667IleMet: 0.667 ± 0.031	0.883IleAsn: 0.883 ± 0.035	2.503IlePro: 2.503 ± 0.058	0.966IleGln: 0.966 ± 0.039	2.813IleArg: 2.813 ± 0.062	2.065IleSer: 2.065 ± 0.052	2.78IleThr: 2.78 ± 0.061	5.126IleVal: 5.126 ± 0.091	0.488IleTrp: 0.488 ± 0.024	0.737IleTyr: 0.737 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	2.378LysAla: 2.378 ± 0.072	0.069LysCys: 0.069 ± 0.009	1.005LysAsp: 1.005 ± 0.042	0.921LysGlu: 0.921 ± 0.044	0.437LysPhe: 0.437 ± 0.025	1.47LysGly: 1.47 ± 0.052	0.412LysHis: 0.412 ± 0.021	0.861LysIle: 0.861 ± 0.038	0.719LysLys: 0.719 ± 0.032	1.711LysLeu: 1.711 ± 0.055	0.364LysMet: 0.364 ± 0.021	0.437LysAsn: 0.437 ± 0.025	1.126LysPro: 1.126 ± 0.04	0.608LysGln: 0.608 ± 0.029	1.526LysArg: 1.526 ± 0.046	0.938LysSer: 0.938 ± 0.036	1.179LysThr: 1.179 ± 0.043	1.602LysVal: 1.602 ± 0.053	0.202LysTrp: 0.202 ± 0.016	0.364LysTyr: 0.364 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	15.108LeuAla: 15.108 ± 0.171	0.457LeuCys: 0.457 ± 0.025	6.349LeuAsp: 6.349 ± 0.115	5.73LeuGlu: 5.73 ± 0.091	2.825LeuPhe: 2.825 ± 0.065	9.447LeuGly: 9.447 ± 0.132	1.743LeuHis: 1.743 ± 0.052	4.495LeuIle: 4.495 ± 0.102	1.65LeuLys: 1.65 ± 0.056	10.152LeuLeu: 10.152 ± 0.141	1.71LeuMet: 1.71 ± 0.049	1.62LeuAsn: 1.62 ± 0.047	5.679LeuPro: 5.679 ± 0.089	2.55LeuGln: 2.55 ± 0.06	7.88LeuArg: 7.88 ± 0.114	5.139LeuSer: 5.139 ± 0.081	6.024LeuThr: 6.024 ± 0.085	9.073LeuVal: 9.073 ± 0.129	1.371LeuTrp: 1.371 ± 0.046	1.636LeuTyr: 1.636 ± 0.05	0.0LeuXaa: 0.0 ± 0.0
Met	2.187MetAla: 2.187 ± 0.047	0.094MetCys: 0.094 ± 0.01	0.849MetAsp: 0.849 ± 0.036	0.647MetGlu: 0.647 ± 0.029	0.549MetPhe: 0.549 ± 0.025	1.408MetGly: 1.408 ± 0.045	0.331MetHis: 0.331 ± 0.023	0.971MetIle: 0.971 ± 0.034	0.409MetLys: 0.409 ± 0.023	1.867MetLeu: 1.867 ± 0.05	0.355MetMet: 0.355 ± 0.022	0.459MetAsn: 0.459 ± 0.022	1.193MetPro: 1.193 ± 0.045	0.556MetGln: 0.556 ± 0.026	1.47MetArg: 1.47 ± 0.041	1.385MetSer: 1.385 ± 0.04	1.749MetThr: 1.749 ± 0.05	1.299MetVal: 1.299 ± 0.042	0.225MetTrp: 0.225 ± 0.016	0.303MetTyr: 0.303 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.408AsnAla: 2.408 ± 0.066	0.095AsnCys: 0.095 ± 0.01	1.027AsnAsp: 1.027 ± 0.037	1.011AsnGlu: 1.011 ± 0.035	0.528AsnPhe: 0.528 ± 0.028	1.783AsnGly: 1.783 ± 0.05	0.298AsnHis: 0.298 ± 0.021	0.778AsnIle: 0.778 ± 0.033	0.337AsnLys: 0.337 ± 0.021	1.75AsnLeu: 1.75 ± 0.046	0.282AsnMet: 0.282 ± 0.018	0.389AsnAsn: 0.389 ± 0.024	1.495AsnPro: 1.495 ± 0.05	0.504AsnGln: 0.504 ± 0.024	1.185AsnArg: 1.185 ± 0.033	0.775AsnSer: 0.775 ± 0.036	0.991AsnThr: 0.991 ± 0.036	1.614AsnVal: 1.614 ± 0.048	0.242AsnTrp: 0.242 ± 0.019	0.411AsnTyr: 0.411 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	8.044ProAla: 8.044 ± 0.142	0.192ProCys: 0.192 ± 0.017	3.994ProAsp: 3.994 ± 0.078	4.375ProGlu: 4.375 ± 0.075	1.683ProPhe: 1.683 ± 0.044	5.36ProGly: 5.36 ± 0.094	1.149ProHis: 1.149 ± 0.039	2.324ProIle: 2.324 ± 0.058	0.98ProLys: 0.98 ± 0.041	5.007ProLeu: 5.007 ± 0.087	0.862ProMet: 0.862 ± 0.032	0.988ProAsn: 0.988 ± 0.032	2.307ProPro: 2.307 ± 0.075	1.676ProGln: 1.676 ± 0.051	3.772ProArg: 3.772 ± 0.073	2.947ProSer: 2.947 ± 0.067	3.225ProThr: 3.225 ± 0.084	4.954ProVal: 4.954 ± 0.101	0.893ProTrp: 0.893 ± 0.034	1.143ProTyr: 1.143 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	3.639GlnAla: 3.639 ± 0.07	0.111GlnCys: 0.111 ± 0.013	1.236GlnAsp: 1.236 ± 0.04	1.399GlnGlu: 1.399 ± 0.047	0.77GlnPhe: 0.77 ± 0.035	2.175GlnGly: 2.175 ± 0.057	0.555GlnHis: 0.555 ± 0.027	1.378GlnIle: 1.378 ± 0.04	0.617GlnLys: 0.617 ± 0.029	3.132GlnLeu: 3.132 ± 0.075	0.479GlnMet: 0.479 ± 0.024	0.566GlnAsn: 0.566 ± 0.026	1.597GlnPro: 1.597 ± 0.06	1.138GlnGln: 1.138 ± 0.043	2.467GlnArg: 2.467 ± 0.057	1.303GlnSer: 1.303 ± 0.042	1.492GlnThr: 1.492 ± 0.047	2.256GlnVal: 2.256 ± 0.059	0.431GlnTrp: 0.431 ± 0.023	0.559GlnTyr: 0.559 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	9.987ArgAla: 9.987 ± 0.15	0.283ArgCys: 0.283 ± 0.019	4.445ArgAsp: 4.445 ± 0.087	5.05ArgGlu: 5.05 ± 0.103	2.462ArgPhe: 2.462 ± 0.06	6.054ArgGly: 6.054 ± 0.101	1.605ArgHis: 1.605 ± 0.043	4.001ArgIle: 4.001 ± 0.071	1.36ArgLys: 1.36 ± 0.044	7.414ArgLeu: 7.414 ± 0.124	1.747ArgMet: 1.747 ± 0.046	1.286ArgAsn: 1.286 ± 0.042	3.858ArgPro: 3.858 ± 0.074	1.965ArgGln: 1.965 ± 0.052	7.133ArgArg: 7.133 ± 0.121	3.713ArgSer: 3.713 ± 0.067	4.135ArgThr: 4.135 ± 0.063	6.548ArgVal: 6.548 ± 0.104	1.189ArgTrp: 1.189 ± 0.048	1.551ArgTyr: 1.551 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	6.279SerAla: 6.279 ± 0.093	0.247SerCys: 0.247 ± 0.02	2.87SerAsp: 2.87 ± 0.067	2.548SerGlu: 2.548 ± 0.066	1.692SerPhe: 1.692 ± 0.045	5.137SerGly: 5.137 ± 0.08	0.905SerHis: 0.905 ± 0.035	2.396SerIle: 2.396 ± 0.055	0.867SerLys: 0.867 ± 0.033	4.875SerLeu: 4.875 ± 0.073	1.073SerMet: 1.073 ± 0.034	0.947SerAsn: 0.947 ± 0.037	2.817SerPro: 2.817 ± 0.068	1.25SerGln: 1.25 ± 0.041	3.425SerArg: 3.425 ± 0.072	2.694SerSer: 2.694 ± 0.059	3.049SerThr: 3.049 ± 0.07	4.01SerVal: 4.01 ± 0.072	0.842SerTrp: 0.842 ± 0.032	1.082SerTyr: 1.082 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	7.623ThrAla: 7.623 ± 0.114	0.261ThrCys: 0.261 ± 0.018	3.328ThrAsp: 3.328 ± 0.063	3.004ThrGlu: 3.004 ± 0.069	1.841ThrPhe: 1.841 ± 0.054	5.736ThrGly: 5.736 ± 0.088	1.092ThrHis: 1.092 ± 0.036	2.987ThrIle: 2.987 ± 0.075	1.042ThrLys: 1.042 ± 0.041	5.815ThrLeu: 5.815 ± 0.088	0.963ThrMet: 0.963 ± 0.035	0.999ThrAsn: 0.999 ± 0.034	3.967ThrPro: 3.967 ± 0.081	1.462ThrGln: 1.462 ± 0.037	3.783ThrArg: 3.783 ± 0.072	2.892ThrSer: 2.892 ± 0.065	3.449ThrThr: 3.449 ± 0.09	5.4ThrVal: 5.4 ± 0.108	0.84ThrTrp: 0.84 ± 0.036	1.13ThrTyr: 1.13 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	12.64ValAla: 12.64 ± 0.168	0.499ValCys: 0.499 ± 0.025	5.469ValAsp: 5.469 ± 0.086	5.002ValGlu: 5.002 ± 0.089	2.835ValPhe: 2.835 ± 0.064	7.122ValGly: 7.122 ± 0.109	1.614ValHis: 1.614 ± 0.047	4.603ValIle: 4.603 ± 0.086	1.524ValLys: 1.524 ± 0.048	9.168ValLeu: 9.168 ± 0.136	1.59ValMet: 1.59 ± 0.053	1.593ValAsn: 1.593 ± 0.05	4.906ValPro: 4.906 ± 0.089	2.116ValGln: 2.116 ± 0.052	6.265ValArg: 6.265 ± 0.095	4.572ValSer: 4.572 ± 0.073	5.623ValThr: 5.623 ± 0.103	8.713ValVal: 8.713 ± 0.13	1.166ValTrp: 1.166 ± 0.036	1.556ValTyr: 1.556 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	1.755TrpAla: 1.755 ± 0.047	0.101TrpCys: 0.101 ± 0.012	0.731TrpAsp: 0.731 ± 0.034	0.657TrpGlu: 0.657 ± 0.026	0.556TrpPhe: 0.556 ± 0.025	1.17TrpGly: 1.17 ± 0.044	0.333TrpHis: 0.333 ± 0.02	0.821TrpIle: 0.821 ± 0.035	0.244TrpLys: 0.244 ± 0.018	1.77TrpLeu: 1.77 ± 0.054	0.325TrpMet: 0.325 ± 0.017	0.384TrpAsn: 0.384 ± 0.022	0.731TrpPro: 0.731 ± 0.031	0.534TrpGln: 0.534 ± 0.026	1.335TrpArg: 1.335 ± 0.043	0.859TrpSer: 0.859 ± 0.038	0.917TrpThr: 0.917 ± 0.035	1.079TrpVal: 1.079 ± 0.041	0.349TrpTrp: 0.349 ± 0.022	0.269TrpTyr: 0.269 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.623TyrAla: 2.623 ± 0.055	0.117TyrCys: 0.117 ± 0.012	1.316TyrAsp: 1.316 ± 0.039	1.193TyrGlu: 1.193 ± 0.044	0.634TyrPhe: 0.634 ± 0.03	1.975TyrGly: 1.975 ± 0.056	0.272TyrHis: 0.272 ± 0.021	0.636TyrIle: 0.636 ± 0.031	0.296TyrLys: 0.296 ± 0.02	2.075TyrLeu: 2.075 ± 0.053	0.282TyrMet: 0.282 ± 0.02	0.438TyrAsn: 0.438 ± 0.025	1.098TyrPro: 1.098 ± 0.045	0.499TyrGln: 0.499 ± 0.029	1.556TyrArg: 1.556 ± 0.052	0.969TyrSer: 0.969 ± 0.037	1.123TyrThr: 1.123 ± 0.044	1.647TyrVal: 1.647 ± 0.045	0.283TyrTrp: 0.283 ± 0.02	0.462TyrTyr: 0.462 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2529 proteins (822206 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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