Nocardia mexicana

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Nocardia

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8181 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A370H8P5|A0A370H8P5_9NOCA Membrane-bound lytic murein transglycosylase B OS=Nocardia mexicana OX=279262 GN=DFR68_103433 PE=4 SV=1
MM1 pKa = 7.74TDD3 pKa = 3.16YY4 pKa = 11.7KK5 pKa = 10.93LFRR8 pKa = 11.84CVQCGFEE15 pKa = 4.07YY16 pKa = 10.91DD17 pKa = 3.92EE18 pKa = 4.49ALGWPEE24 pKa = 5.24DD25 pKa = 4.78DD26 pKa = 3.95IAPGTRR32 pKa = 11.84WDD34 pKa = 5.68DD35 pKa = 4.13IPDD38 pKa = 3.72DD39 pKa = 4.45WSCPDD44 pKa = 3.97CGAAKK49 pKa = 10.43ADD51 pKa = 3.73FEE53 pKa = 4.42MVEE56 pKa = 4.14VSRR59 pKa = 11.84AA60 pKa = 3.2

Molecular weight: 6.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A370GXJ8|A0A370GXJ8_9NOCA Phosphate acetyltransferase OS=Nocardia mexicana OX=279262 GN=DFR68_1107 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84QKK40 pKa = 10.6GRR42 pKa = 11.84AKK44 pKa = 9.64LTAA47 pKa = 4.21

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8181

0

8181

2651363

29

11446

324.1

34.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.269 ± 0.036

0.76 ± 0.008

6.241 ± 0.025

5.457 ± 0.029

2.914 ± 0.018

9.066 ± 0.028

2.252 ± 0.014

3.995 ± 0.017

1.83 ± 0.02

10.029 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.92 ± 0.012

1.971 ± 0.015

6.07 ± 0.026

2.875 ± 0.018

8.035 ± 0.034

5.214 ± 0.019

6.088 ± 0.021

8.44 ± 0.031

1.489 ± 0.011

2.086 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski