Microcystis phage Mic1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5TS47|A0A4Y5TS47_9CAUD Ribonucleotide reductase OS=Microcystis phage Mic1 OX=2587456 GN=Mic1_75 PE=4 SV=1
MM1 pKa = 7.41TNTVNGEE8 pKa = 4.16LTWFRR13 pKa = 11.84ILGQDD18 pKa = 3.2WYY20 pKa = 11.13GKK22 pKa = 7.34TCIMDD27 pKa = 3.67IALNGYY33 pKa = 9.91DD34 pKa = 5.38IEE36 pKa = 4.68DD37 pKa = 4.2LEE39 pKa = 4.37NLGLGLGEE47 pKa = 4.25SEE49 pKa = 4.12KK50 pKa = 10.64EE51 pKa = 3.7ARR53 pKa = 11.84EE54 pKa = 4.72VINQWLLALDD64 pKa = 3.96IYY66 pKa = 10.87DD67 pKa = 4.03PQGFWSVDD75 pKa = 3.24LSSITDD81 pKa = 3.9FDD83 pKa = 4.22CQIEE87 pKa = 4.29LSDD90 pKa = 3.66GCYY93 pKa = 10.73VSVDD97 pKa = 3.03WEE99 pKa = 4.32DD100 pKa = 3.38EE101 pKa = 4.08KK102 pKa = 11.73NYY104 pKa = 11.0AKK106 pKa = 10.59YY107 pKa = 10.46RR108 pKa = 11.84EE109 pKa = 4.19ITDD112 pKa = 3.34NN113 pKa = 3.64

Molecular weight: 13.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5TPT8|A0A4Y5TPT8_9CAUD Uncharacterized protein OS=Microcystis phage Mic1 OX=2587456 GN=Mic1_18 PE=4 SV=1
MM1 pKa = 7.71TNPQPKK7 pKa = 7.81PQRR10 pKa = 11.84VYY12 pKa = 7.47VTRR15 pKa = 11.84YY16 pKa = 8.69ALTSGVYY23 pKa = 8.24RR24 pKa = 11.84TNLYY28 pKa = 10.66SSNLYY33 pKa = 8.36YY34 pKa = 10.38TAFVKK39 pKa = 10.59NPQGAIVTLKK49 pKa = 10.25FGKK52 pKa = 10.02SVFLTYY58 pKa = 10.85EE59 pKa = 3.76EE60 pKa = 4.22AKK62 pKa = 10.54KK63 pKa = 10.77DD64 pKa = 3.64FEE66 pKa = 4.23KK67 pKa = 10.81RR68 pKa = 11.84KK69 pKa = 10.34AFAIRR74 pKa = 11.84CAEE77 pKa = 4.12RR78 pKa = 11.84KK79 pKa = 9.14LQNLKK84 pKa = 10.34NLEE87 pKa = 4.1FSFTEE92 pKa = 4.4CC93 pKa = 4.84

Molecular weight: 10.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

98

0

98

23825

45

2572

243.1

27.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.696 ± 0.422

1.079 ± 0.143

5.167 ± 0.186

6.728 ± 0.338

5.125 ± 0.217

5.927 ± 0.339

1.494 ± 0.172

6.749 ± 0.255

7.11 ± 0.377

10.279 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.658 ± 0.147

6.195 ± 0.25

3.899 ± 0.24

4.101 ± 0.307

3.836 ± 0.118

7.941 ± 0.321

6.023 ± 0.256

6.027 ± 0.241

1.305 ± 0.147

3.66 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski