Halorubrum sp. J07HR59

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halorubrum; unclassified Halorubrum

Average proteome isoelectric point is 5.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1832 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U1P133|U1P133_9EURY Transposase IS605 OrfB family central region OS=Halorubrum sp. J07HR59 OX=1238428 GN=J07HR59_00976 PE=4 SV=1
MM1 pKa = 8.31LEE3 pKa = 4.39IGQSAPDD10 pKa = 3.41FTLPNQHH17 pKa = 6.34GEE19 pKa = 4.24QVSLSAHH26 pKa = 6.62DD27 pKa = 4.12GFAVVYY33 pKa = 6.83FFPRR37 pKa = 11.84ADD39 pKa = 3.73TPGCTAEE46 pKa = 4.07ACSFRR51 pKa = 11.84DD52 pKa = 3.27EE53 pKa = 4.29WEE55 pKa = 4.14SFAEE59 pKa = 3.85RR60 pKa = 11.84DD61 pKa = 3.43VSVFGISDD69 pKa = 3.99DD70 pKa = 3.96PVSDD74 pKa = 4.58LDD76 pKa = 5.41AFAADD81 pKa = 3.87YY82 pKa = 11.06DD83 pKa = 4.33LQFHH87 pKa = 6.98LLSDD91 pKa = 3.64TDD93 pKa = 4.16GDD95 pKa = 3.84VSASYY100 pKa = 11.09GSYY103 pKa = 10.88GEE105 pKa = 4.31KK106 pKa = 10.58

Molecular weight: 11.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U1NK27|U1NK27_9EURY tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS OS=Halorubrum sp. J07HR59 OX=1238428 GN=tiaS PE=3 SV=1
MM1 pKa = 7.69IFTTVHH7 pKa = 5.61SSPRR11 pKa = 11.84KK12 pKa = 8.35RR13 pKa = 11.84NEE15 pKa = 3.53PEE17 pKa = 3.87TILNGRR23 pKa = 11.84GQCNLVPTSQDD34 pKa = 2.84STTTQSHH41 pKa = 6.22SPSQSCHH48 pKa = 5.76GGCLTGAKK56 pKa = 9.63RR57 pKa = 11.84PLSNRR62 pKa = 11.84PPVTTRR68 pKa = 11.84SGSLTVYY75 pKa = 10.46AGVSIVASHH84 pKa = 6.22VGKK87 pKa = 9.77NAQSIVVNTPPVRR100 pKa = 11.84WMAVANDD107 pKa = 4.62AIVTEE112 pKa = 4.43RR113 pKa = 11.84PVLRR117 pKa = 11.84EE118 pKa = 3.73RR119 pKa = 11.84STGRR123 pKa = 11.84YY124 pKa = 8.52LVASSEE130 pKa = 4.66SITGSRR136 pKa = 11.84YY137 pKa = 9.03VAPLSTALFKK147 pKa = 10.27PHH149 pKa = 5.37QRR151 pKa = 11.84KK152 pKa = 7.63VTHH155 pKa = 6.49NGG157 pKa = 2.88

Molecular weight: 16.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1832

0

1832

432529

23

2514

236.1

25.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.408 ± 0.071

0.867 ± 0.019

7.904 ± 0.063

7.698 ± 0.073

3.13 ± 0.04

8.471 ± 0.059

2.094 ± 0.03

4.411 ± 0.042

1.842 ± 0.032

8.469 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.819 ± 0.026

2.45 ± 0.033

4.919 ± 0.038

3.306 ± 0.035

6.797 ± 0.058

6.407 ± 0.061

6.835 ± 0.055

8.486 ± 0.058

1.085 ± 0.022

2.465 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski