Aspergillus sydowii CBS 593.65
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13568 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L9T8G8|A0A1L9T8G8_9EURO Uncharacterized protein OS=Aspergillus sydowii CBS 593.65 OX=1036612 GN=ASPSYDRAFT_158217 PE=4 SV=1
MM1 pKa = 7.55 ILSKK5 pKa = 10.09 MKK7 pKa = 10.74 SSFLPSAVVSLLSIPGVMAACSLPSTYY34 pKa = 10.45 SWTSTDD40 pKa = 3.71 ALADD44 pKa = 3.7 PAAGQTALKK53 pKa = 10.53 DD54 pKa = 3.56 FTAVVSNGQHH64 pKa = 5.2 IVYY67 pKa = 10.4 ASTADD72 pKa = 3.08 EE73 pKa = 4.46 GGNYY77 pKa = 10.39 GSMVFGAFSEE87 pKa = 4.29 WSEE90 pKa = 4.12 MASASQNAMDD100 pKa = 4.84 FAAVAPTLFYY110 pKa = 10.44 FSPKK114 pKa = 10.13 DD115 pKa = 3.21 IWILAYY121 pKa = 10.09 QWGSTAFSYY130 pKa = 8.68 RR131 pKa = 11.84 TSSDD135 pKa = 3.23 PTDD138 pKa = 3.6 ANGWSSEE145 pKa = 3.82 EE146 pKa = 3.9 ALFSGTITGSDD157 pKa = 3.18 TGAIDD162 pKa = 3.29 QTVIGDD168 pKa = 3.88 DD169 pKa = 3.2 TNMYY173 pKa = 10.82 LFFAGDD179 pKa = 2.94 NGKK182 pKa = 9.64 IYY184 pKa = 9.95 RR185 pKa = 11.84 ASMPIDD191 pKa = 3.76 NFPGDD196 pKa = 3.52 FGTDD200 pKa = 3.61 FEE202 pKa = 5.84 EE203 pKa = 5.17 IMSDD207 pKa = 2.91 TSNNLFEE214 pKa = 4.78 AVQVYY219 pKa = 7.91 TVDD222 pKa = 3.37 GQNQYY227 pKa = 11.61 LMIVEE232 pKa = 5.46 AIGSEE237 pKa = 3.63 GRR239 pKa = 11.84 YY240 pKa = 9.2 FRR242 pKa = 11.84 SFVADD247 pKa = 3.43 SLGGEE252 pKa = 4.28 WTSQGSTEE260 pKa = 4.17 DD261 pKa = 3.25 QPFAGKK267 pKa = 10.33 ANSGATWTNDD277 pKa = 2.46 ISHH280 pKa = 7.12 GDD282 pKa = 3.84 LVRR285 pKa = 11.84 SGPDD289 pKa = 2.68 QTNPVDD295 pKa = 3.68 PCNLQLLYY303 pKa = 10.44 QGKK306 pKa = 9.78 DD307 pKa = 3.15 PSANGDD313 pKa = 3.86 YY314 pKa = 8.84 NTQPWRR320 pKa = 11.84 PGVLTLNAA328 pKa = 4.47
Molecular weight: 35.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.821
IPC_protein 3.846
Toseland 3.617
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.783
Rodwell 3.668
Grimsley 3.516
Solomon 3.834
Lehninger 3.783
Nozaki 3.948
DTASelect 4.215
Thurlkill 3.668
EMBOSS 3.795
Sillero 3.961
Patrickios 1.189
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.846
Protein with the highest isoelectric point:
>tr|A0A1L9TZK7|A0A1L9TZK7_9EURO Uncharacterized protein OS=Aspergillus sydowii CBS 593.65 OX=1036612 GN=ASPSYDRAFT_1156792 PE=4 SV=1
SS1 pKa = 6.27 QKK3 pKa = 10.7 SFRR6 pKa = 11.84 TKK8 pKa = 10.38 QKK10 pKa = 9.84 LAKK13 pKa = 9.55 AQKK16 pKa = 8.59 QNRR19 pKa = 11.84 PIPQWIRR26 pKa = 11.84 LRR28 pKa = 11.84 TGNTIRR34 pKa = 11.84 YY35 pKa = 5.79 NAKK38 pKa = 8.89 RR39 pKa = 11.84 RR40 pKa = 11.84 HH41 pKa = 4.14 WRR43 pKa = 11.84 KK44 pKa = 7.41 TRR46 pKa = 11.84 LGII49 pKa = 4.46
Molecular weight: 6.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.39
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.34
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.076
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13568
0
13568
6311667
49
6237
465.2
51.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.413 ± 0.017
1.354 ± 0.008
5.635 ± 0.016
5.995 ± 0.019
3.852 ± 0.012
6.998 ± 0.02
2.402 ± 0.009
5.062 ± 0.013
4.409 ± 0.019
9.129 ± 0.021
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.124 ± 0.007
3.683 ± 0.009
5.983 ± 0.02
3.96 ± 0.014
6.125 ± 0.019
8.243 ± 0.023
5.928 ± 0.012
6.241 ± 0.014
1.542 ± 0.007
2.91 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here