Amino acid dipepetide frequency for Frigoribacterium sp. JB110

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.344AlaAla: 19.344 ± 0.198
0.771AlaCys: 0.771 ± 0.029
8.593AlaAsp: 8.593 ± 0.105
8.706AlaGlu: 8.706 ± 0.106
3.879AlaPhe: 3.879 ± 0.062
12.123AlaGly: 12.123 ± 0.122
2.609AlaHis: 2.609 ± 0.061
5.883AlaIle: 5.883 ± 0.087
2.482AlaLys: 2.482 ± 0.053
13.551AlaLeu: 13.551 ± 0.139
3.004AlaMet: 3.004 ± 0.053
2.184AlaAsn: 2.184 ± 0.05
6.332AlaPro: 6.332 ± 0.11
3.925AlaGln: 3.925 ± 0.06
8.976AlaArg: 8.976 ± 0.117
7.309AlaSer: 7.309 ± 0.093
7.06AlaThr: 7.06 ± 0.073
11.477AlaVal: 11.477 ± 0.132
1.862AlaTrp: 1.862 ± 0.044
2.412AlaTyr: 2.412 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
0.714CysAla: 0.714 ± 0.028
0.055CysCys: 0.055 ± 0.007
0.347CysAsp: 0.347 ± 0.018
0.272CysGlu: 0.272 ± 0.016
0.181CysPhe: 0.181 ± 0.013
0.608CysGly: 0.608 ± 0.026
0.114CysHis: 0.114 ± 0.01
0.192CysIle: 0.192 ± 0.011
0.061CysLys: 0.061 ± 0.008
0.432CysLeu: 0.432 ± 0.023
0.084CysMet: 0.084 ± 0.011
0.098CysAsn: 0.098 ± 0.01
0.239CysPro: 0.239 ± 0.016
0.112CysGln: 0.112 ± 0.01
0.33CysArg: 0.33 ± 0.016
0.353CysSer: 0.353 ± 0.016
0.33CysThr: 0.33 ± 0.019
0.452CysVal: 0.452 ± 0.02
0.065CysTrp: 0.065 ± 0.007
0.105CysTyr: 0.105 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
9.493AspAla: 9.493 ± 0.117
0.244AspCys: 0.244 ± 0.017
5.279AspAsp: 5.279 ± 0.094
5.422AspGlu: 5.422 ± 0.087
1.719AspPhe: 1.719 ± 0.048
6.845AspGly: 6.845 ± 0.113
1.252AspHis: 1.252 ± 0.037
2.668AspIle: 2.668 ± 0.048
0.955AspLys: 0.955 ± 0.032
6.07AspLeu: 6.07 ± 0.072
0.922AspMet: 0.922 ± 0.03
0.954AspAsn: 0.954 ± 0.035
4.231AspPro: 4.231 ± 0.089
1.679AspGln: 1.679 ± 0.044
4.55AspArg: 4.55 ± 0.074
2.866AspSer: 2.866 ± 0.054
3.101AspThr: 3.101 ± 0.06
5.974AspVal: 5.974 ± 0.077
0.906AspTrp: 0.906 ± 0.03
1.22AspTyr: 1.22 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
7.415GluAla: 7.415 ± 0.098
0.217GluCys: 0.217 ± 0.013
2.952GluAsp: 2.952 ± 0.06
3.445GluGlu: 3.445 ± 0.062
1.873GluPhe: 1.873 ± 0.043
4.022GluGly: 4.022 ± 0.067
1.835GluHis: 1.835 ± 0.042
2.98GluIle: 2.98 ± 0.059
1.398GluLys: 1.398 ± 0.041
6.723GluLeu: 6.723 ± 0.085
1.157GluMet: 1.157 ± 0.034
1.474GluAsn: 1.474 ± 0.043
3.399GluPro: 3.399 ± 0.074
2.597GluGln: 2.597 ± 0.048
5.622GluArg: 5.622 ± 0.096
3.198GluSer: 3.198 ± 0.056
3.462GluThr: 3.462 ± 0.069
4.57GluVal: 4.57 ± 0.074
0.991GluTrp: 0.991 ± 0.026
1.274GluTyr: 1.274 ± 0.038
0.0GluXaa: 0.0 ± 0.0
Phe
4.195PheAla: 4.195 ± 0.079
0.182PheCys: 0.182 ± 0.013
2.557PheAsp: 2.557 ± 0.043
1.753PheGlu: 1.753 ± 0.043
1.069PhePhe: 1.069 ± 0.039
3.322PheGly: 3.322 ± 0.058
0.569PheHis: 0.569 ± 0.021
1.141PheIle: 1.141 ± 0.031
0.374PheLys: 0.374 ± 0.022
2.727PheLeu: 2.727 ± 0.061
0.46PheMet: 0.46 ± 0.021
0.575PheAsn: 0.575 ± 0.024
1.385PhePro: 1.385 ± 0.035
0.743PheGln: 0.743 ± 0.029
1.811PheArg: 1.811 ± 0.036
1.838PheSer: 1.838 ± 0.044
1.992PheThr: 1.992 ± 0.047
2.891PheVal: 2.891 ± 0.058
0.442PheTrp: 0.442 ± 0.022
0.617PheTyr: 0.617 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
11.447GlyAla: 11.447 ± 0.128
0.6GlyCys: 0.6 ± 0.029
5.73GlyAsp: 5.73 ± 0.094
5.733GlyGlu: 5.733 ± 0.089
3.174GlyPhe: 3.174 ± 0.062
7.979GlyGly: 7.979 ± 0.117
1.779GlyHis: 1.779 ± 0.041
4.726GlyIle: 4.726 ± 0.07
1.945GlyLys: 1.945 ± 0.047
8.466GlyLeu: 8.466 ± 0.113
2.138GlyMet: 2.138 ± 0.045
1.679GlyAsn: 1.679 ± 0.045
3.519GlyPro: 3.519 ± 0.054
2.509GlyGln: 2.509 ± 0.055
6.425GlyArg: 6.425 ± 0.091
5.555GlySer: 5.555 ± 0.07
5.76GlyThr: 5.76 ± 0.114
7.698GlyVal: 7.698 ± 0.091
1.612GlyTrp: 1.612 ± 0.044
2.186GlyTyr: 2.186 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
2.539HisAla: 2.539 ± 0.053
0.117HisCys: 0.117 ± 0.01
1.523HisAsp: 1.523 ± 0.041
1.263HisGlu: 1.263 ± 0.039
0.52HisPhe: 0.52 ± 0.023
2.137HisGly: 2.137 ± 0.049
0.6HisHis: 0.6 ± 0.026
0.789HisIle: 0.789 ± 0.03
0.28HisLys: 0.28 ± 0.015
2.031HisLeu: 2.031 ± 0.047
0.346HisMet: 0.346 ± 0.018
0.374HisAsn: 0.374 ± 0.018
1.476HisPro: 1.476 ± 0.045
0.537HisGln: 0.537 ± 0.021
1.634HisArg: 1.634 ± 0.042
1.041HisSer: 1.041 ± 0.033
1.099HisThr: 1.099 ± 0.036
1.72HisVal: 1.72 ± 0.039
0.277HisTrp: 0.277 ± 0.017
0.38HisTyr: 0.38 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
7.461IleAla: 7.461 ± 0.088
0.252IleCys: 0.252 ± 0.015
3.846IleAsp: 3.846 ± 0.053
2.963IleGlu: 2.963 ± 0.055
1.076IlePhe: 1.076 ± 0.034
4.832IleGly: 4.832 ± 0.076
0.657IleHis: 0.657 ± 0.025
1.963IleIle: 1.963 ± 0.055
0.645IleLys: 0.645 ± 0.026
3.505IleLeu: 3.505 ± 0.058
0.666IleMet: 0.666 ± 0.028
0.876IleAsn: 0.876 ± 0.027
2.222IlePro: 2.222 ± 0.047
0.907IleGln: 0.907 ± 0.031
3.213IleArg: 3.213 ± 0.061
2.492IleSer: 2.492 ± 0.044
2.872IleThr: 2.872 ± 0.057
4.911IleVal: 4.911 ± 0.076
0.479IleTrp: 0.479 ± 0.023
0.697IleTyr: 0.697 ± 0.023
0.0IleXaa: 0.0 ± 0.0
Lys
2.167LysAla: 2.167 ± 0.053
0.07LysCys: 0.07 ± 0.008
0.974LysAsp: 0.974 ± 0.033
0.919LysGlu: 0.919 ± 0.034
0.46LysPhe: 0.46 ± 0.018
1.354LysGly: 1.354 ± 0.039
0.466LysHis: 0.466 ± 0.021
0.873LysIle: 0.873 ± 0.029
0.709LysLys: 0.709 ± 0.03
1.72LysLeu: 1.72 ± 0.045
0.384LysMet: 0.384 ± 0.019
0.507LysAsn: 0.507 ± 0.024
1.077LysPro: 1.077 ± 0.037
0.693LysGln: 0.693 ± 0.023
1.584LysArg: 1.584 ± 0.038
1.06LysSer: 1.06 ± 0.036
1.193LysThr: 1.193 ± 0.038
1.446LysVal: 1.446 ± 0.04
0.212LysTrp: 0.212 ± 0.013
0.361LysTyr: 0.361 ± 0.019
0.0LysXaa: 0.0 ± 0.0
Leu
13.596LeuAla: 13.596 ± 0.142
0.531LeuCys: 0.531 ± 0.021
6.65LeuAsp: 6.65 ± 0.087
5.115LeuGlu: 5.115 ± 0.075
2.78LeuPhe: 2.78 ± 0.052
8.917LeuGly: 8.917 ± 0.103
1.848LeuHis: 1.848 ± 0.047
4.691LeuIle: 4.691 ± 0.076
1.58LeuLys: 1.58 ± 0.044
9.599LeuLeu: 9.599 ± 0.132
1.789LeuMet: 1.789 ± 0.042
1.821LeuAsn: 1.821 ± 0.042
5.224LeuPro: 5.224 ± 0.08
2.32LeuGln: 2.32 ± 0.043
7.26LeuArg: 7.26 ± 0.096
5.877LeuSer: 5.877 ± 0.079
6.191LeuThr: 6.191 ± 0.075
8.792LeuVal: 8.792 ± 0.094
1.214LeuTrp: 1.214 ± 0.034
1.51LeuTyr: 1.51 ± 0.041
0.0LeuXaa: 0.0 ± 0.0
Met
2.226MetAla: 2.226 ± 0.042
0.094MetCys: 0.094 ± 0.01
0.844MetAsp: 0.844 ± 0.03
0.68MetGlu: 0.68 ± 0.024
0.613MetPhe: 0.613 ± 0.025
1.484MetGly: 1.484 ± 0.037
0.427MetHis: 0.427 ± 0.021
1.05MetIle: 1.05 ± 0.034
0.438MetLys: 0.438 ± 0.022
2.09MetLeu: 2.09 ± 0.049
0.418MetMet: 0.418 ± 0.022
0.522MetAsn: 0.522 ± 0.021
1.213MetPro: 1.213 ± 0.039
0.588MetGln: 0.588 ± 0.021
1.626MetArg: 1.626 ± 0.04
1.645MetSer: 1.645 ± 0.041
1.845MetThr: 1.845 ± 0.038
1.436MetVal: 1.436 ± 0.039
0.244MetTrp: 0.244 ± 0.016
0.335MetTyr: 0.335 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.513AsnAla: 2.513 ± 0.052
0.096AsnCys: 0.096 ± 0.009
1.274AsnAsp: 1.274 ± 0.042
1.111AsnGlu: 1.111 ± 0.032
0.568AsnPhe: 0.568 ± 0.025
2.04AsnGly: 2.04 ± 0.055
0.347AsnHis: 0.347 ± 0.016
0.848AsnIle: 0.848 ± 0.028
0.35AsnLys: 0.35 ± 0.02
1.788AsnLeu: 1.788 ± 0.04
0.347AsnMet: 0.347 ± 0.017
0.426AsnAsn: 0.426 ± 0.021
1.464AsnPro: 1.464 ± 0.037
0.555AsnGln: 0.555 ± 0.024
1.385AsnArg: 1.385 ± 0.037
0.949AsnSer: 0.949 ± 0.028
1.065AsnThr: 1.065 ± 0.034
1.666AsnVal: 1.666 ± 0.045
0.291AsnTrp: 0.291 ± 0.018
0.41AsnTyr: 0.41 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
6.331ProAla: 6.331 ± 0.102
0.167ProCys: 0.167 ± 0.013
4.582ProAsp: 4.582 ± 0.078
3.785ProGlu: 3.785 ± 0.067
1.683ProPhe: 1.683 ± 0.04
4.836ProGly: 4.836 ± 0.102
1.13ProHis: 1.13 ± 0.034
2.045ProIle: 2.045 ± 0.035
0.987ProLys: 0.987 ± 0.03
4.515ProLeu: 4.515 ± 0.07
0.958ProMet: 0.958 ± 0.027
1.035ProAsn: 1.035 ± 0.034
1.991ProPro: 1.991 ± 0.061
1.568ProGln: 1.568 ± 0.036
3.302ProArg: 3.302 ± 0.059
2.996ProSer: 2.996 ± 0.051
3.2ProThr: 3.2 ± 0.056
4.618ProVal: 4.618 ± 0.075
0.789ProTrp: 0.789 ± 0.03
1.025ProTyr: 1.025 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
3.577GlnAla: 3.577 ± 0.063
0.112GlnCys: 0.112 ± 0.009
1.319GlnAsp: 1.319 ± 0.034
1.476GlnGlu: 1.476 ± 0.039
0.815GlnPhe: 0.815 ± 0.029
2.025GlnGly: 2.025 ± 0.044
0.676GlnHis: 0.676 ± 0.025
1.509GlnIle: 1.509 ± 0.039
0.65GlnLys: 0.65 ± 0.025
3.101GlnLeu: 3.101 ± 0.05
0.591GlnMet: 0.591 ± 0.024
0.721GlnAsn: 0.721 ± 0.026
1.488GlnPro: 1.488 ± 0.045
1.35GlnGln: 1.35 ± 0.043
2.523GlnArg: 2.523 ± 0.046
1.522GlnSer: 1.522 ± 0.035
1.575GlnThr: 1.575 ± 0.041
2.318GlnVal: 2.318 ± 0.044
0.414GlnTrp: 0.414 ± 0.022
0.59GlnTyr: 0.59 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
8.941ArgAla: 8.941 ± 0.105
0.322ArgCys: 0.322 ± 0.019
4.537ArgAsp: 4.537 ± 0.075
4.837ArgGlu: 4.837 ± 0.077
2.446ArgPhe: 2.446 ± 0.046
5.627ArgGly: 5.627 ± 0.086
1.604ArgHis: 1.604 ± 0.042
3.89ArgIle: 3.89 ± 0.066
1.336ArgLys: 1.336 ± 0.04
7.06ArgLeu: 7.06 ± 0.107
1.934ArgMet: 1.934 ± 0.041
1.39ArgAsn: 1.39 ± 0.037
3.38ArgPro: 3.38 ± 0.069
1.961ArgGln: 1.961 ± 0.044
6.743ArgArg: 6.743 ± 0.099
4.344ArgSer: 4.344 ± 0.065
4.394ArgThr: 4.394 ± 0.074
6.145ArgVal: 6.145 ± 0.098
1.179ArgTrp: 1.179 ± 0.034
1.473ArgTyr: 1.473 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
7.233SerAla: 7.233 ± 0.098
0.3SerCys: 0.3 ± 0.018
3.62SerAsp: 3.62 ± 0.065
3.093SerGlu: 3.093 ± 0.048
1.858SerPhe: 1.858 ± 0.044
6.398SerGly: 6.398 ± 0.086
1.136SerHis: 1.136 ± 0.03
2.599SerIle: 2.599 ± 0.053
1.011SerLys: 1.011 ± 0.031
5.466SerLeu: 5.466 ± 0.082
1.211SerMet: 1.211 ± 0.031
1.099SerAsn: 1.099 ± 0.032
2.947SerPro: 2.947 ± 0.051
1.458SerGln: 1.458 ± 0.035
3.864SerArg: 3.864 ± 0.06
3.515SerSer: 3.515 ± 0.067
3.47SerThr: 3.47 ± 0.057
4.875SerVal: 4.875 ± 0.059
0.885SerTrp: 0.885 ± 0.031
1.12SerTyr: 1.12 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
7.728ThrAla: 7.728 ± 0.09
0.287ThrCys: 0.287 ± 0.017
3.894ThrAsp: 3.894 ± 0.086
3.292ThrGlu: 3.292 ± 0.067
1.883ThrPhe: 1.883 ± 0.041
6.135ThrGly: 6.135 ± 0.072
1.259ThrHis: 1.259 ± 0.039
3.065ThrIle: 3.065 ± 0.064
1.05ThrLys: 1.05 ± 0.031
5.631ThrLeu: 5.631 ± 0.068
1.128ThrMet: 1.128 ± 0.035
1.155ThrAsn: 1.155 ± 0.036
3.493ThrPro: 3.493 ± 0.055
1.524ThrGln: 1.524 ± 0.04
3.702ThrArg: 3.702 ± 0.063
3.422ThrSer: 3.422 ± 0.058
3.772ThrThr: 3.772 ± 0.073
5.687ThrVal: 5.687 ± 0.071
0.863ThrTrp: 0.863 ± 0.029
1.116ThrTyr: 1.116 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
11.518ValAla: 11.518 ± 0.113
0.511ValCys: 0.511 ± 0.023
5.692ValAsp: 5.692 ± 0.079
4.884ValGlu: 4.884 ± 0.069
2.864ValPhe: 2.864 ± 0.055
7.014ValGly: 7.014 ± 0.09
1.752ValHis: 1.752 ± 0.035
4.257ValIle: 4.257 ± 0.079
1.45ValLys: 1.45 ± 0.044
9.235ValLeu: 9.235 ± 0.11
1.684ValMet: 1.684 ± 0.046
1.743ValAsn: 1.743 ± 0.043
4.774ValPro: 4.774 ± 0.072
2.14ValGln: 2.14 ± 0.046
6.115ValArg: 6.115 ± 0.083
5.265ValSer: 5.265 ± 0.071
5.803ValThr: 5.803 ± 0.08
8.487ValVal: 8.487 ± 0.12
1.19ValTrp: 1.19 ± 0.031
1.45ValTyr: 1.45 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
1.634TrpAla: 1.634 ± 0.038
0.107TrpCys: 0.107 ± 0.01
0.731TrpAsp: 0.731 ± 0.027
0.699TrpGlu: 0.699 ± 0.026
0.524TrpPhe: 0.524 ± 0.023
1.065TrpGly: 1.065 ± 0.033
0.355TrpHis: 0.355 ± 0.021
0.709TrpIle: 0.709 ± 0.026
0.258TrpLys: 0.258 ± 0.017
1.671TrpLeu: 1.671 ± 0.048
0.306TrpMet: 0.306 ± 0.017
0.452TrpAsn: 0.452 ± 0.019
0.705TrpPro: 0.705 ± 0.027
0.602TrpGln: 0.602 ± 0.027
1.29TrpArg: 1.29 ± 0.038
0.877TrpSer: 0.877 ± 0.028
0.873TrpThr: 0.873 ± 0.027
1.085TrpVal: 1.085 ± 0.035
0.333TrpTrp: 0.333 ± 0.02
0.272TrpTyr: 0.272 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.41TyrAla: 2.41 ± 0.045
0.111TyrCys: 0.111 ± 0.008
1.402TyrAsp: 1.402 ± 0.043
1.157TyrGlu: 1.157 ± 0.035
0.658TyrPhe: 0.658 ± 0.024
1.896TyrGly: 1.896 ± 0.045
0.268TyrHis: 0.268 ± 0.015
0.658TyrIle: 0.658 ± 0.025
0.27TyrLys: 0.27 ± 0.015
1.923TyrLeu: 1.923 ± 0.042
0.293TyrMet: 0.293 ± 0.018
0.409TyrAsn: 0.409 ± 0.023
0.983TyrPro: 0.983 ± 0.03
0.586TyrGln: 0.586 ± 0.025
1.587TyrArg: 1.587 ± 0.039
1.031TyrSer: 1.031 ± 0.033
1.029TyrThr: 1.029 ± 0.035
1.582TyrVal: 1.582 ± 0.039
0.301TyrTrp: 0.301 ± 0.018
0.447TyrTyr: 0.447 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3430 proteins (1120900 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski