Tranquillimonas alkanivorans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Tranquillimonas

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4454 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I5U2P1|A0A1I5U2P1_9RHOB Phosphatidate phosphatase APP1 OS=Tranquillimonas alkanivorans OX=441119 GN=SAMN04488047_11713 PE=4 SV=1
MM1 pKa = 7.29KK2 pKa = 9.99AAAIITAGGLALAGAALAQDD22 pKa = 4.21AAPFGQEE29 pKa = 3.43EE30 pKa = 4.88DD31 pKa = 3.43VTYY34 pKa = 10.68AAQLWDD40 pKa = 4.29DD41 pKa = 3.88MVEE44 pKa = 3.9ADD46 pKa = 4.6LAGEE50 pKa = 4.36GAVQSFPYY58 pKa = 10.14PGTDD62 pKa = 3.03PHH64 pKa = 8.71GMMLEE69 pKa = 4.21TFYY72 pKa = 11.62ASATVEE78 pKa = 4.14GHH80 pKa = 5.46TGALIVKK87 pKa = 9.64RR88 pKa = 11.84NYY90 pKa = 9.78GPEE93 pKa = 4.1GVTVDD98 pKa = 3.85QVLGAPSEE106 pKa = 4.12HH107 pKa = 6.58LGAVTVMFQRR117 pKa = 11.84EE118 pKa = 4.13DD119 pKa = 3.89GYY121 pKa = 11.49DD122 pKa = 3.47PEE124 pKa = 4.53TNNWFWAKK132 pKa = 10.13YY133 pKa = 10.21LPDD136 pKa = 3.61GTLDD140 pKa = 3.73RR141 pKa = 11.84NPNDD145 pKa = 3.23MALAGLVGKK154 pKa = 9.99GADD157 pKa = 3.46AGCIACHH164 pKa = 5.65QGAGGEE170 pKa = 4.6DD171 pKa = 3.65YY172 pKa = 11.32LFTTDD177 pKa = 4.24ADD179 pKa = 4.16LAVAEE184 pKa = 4.44

Molecular weight: 19.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I5TE59|A0A1I5TE59_9RHOB Predicted thiol-disulfide oxidoreductase YuxK DCC family OS=Tranquillimonas alkanivorans OX=441119 GN=SAMN04488047_11376 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4454

0

4454

1345483

26

1732

302.1

32.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.674 ± 0.061

0.859 ± 0.011

6.086 ± 0.029

6.57 ± 0.032

3.594 ± 0.025

8.743 ± 0.032

2.067 ± 0.017

4.584 ± 0.024

2.714 ± 0.031

10.112 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.611 ± 0.016

2.218 ± 0.018

5.325 ± 0.025

2.923 ± 0.022

7.566 ± 0.043

4.894 ± 0.028

5.258 ± 0.022

7.634 ± 0.032

1.442 ± 0.016

2.126 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski