Wenzhou yanvirus-like virus 1
Average proteome isoelectric point is 8.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KL64|A0A1L3KL64_9VIRU RNA-dependent RNA polymerase OS=Wenzhou yanvirus-like virus 1 OX=1923683 PE=4 SV=1
MM1 pKa = 7.71 VDD3 pKa = 2.92 QCRR6 pKa = 11.84 FLGLSDD12 pKa = 3.53 FPIRR16 pKa = 11.84 VMGPVGTVSHH26 pKa = 5.74 YY27 pKa = 10.8 VCGKK31 pKa = 8.86 PRR33 pKa = 11.84 IDD35 pKa = 3.98 GACLLYY41 pKa = 10.44 IPARR45 pKa = 11.84 PPLVPLAHH53 pKa = 6.0 WTIAVGGQSYY63 pKa = 10.87 DD64 pKa = 3.69 YY65 pKa = 10.93 ADD67 pKa = 3.97 PAALGGVVLYY77 pKa = 11.07 SDD79 pKa = 4.98 IPPISYY85 pKa = 9.64 NPHH88 pKa = 5.18 VFWHH92 pKa = 6.51 AGSCKK97 pKa = 10.23 SATLQFWMRR106 pKa = 11.84 AGNGCRR112 pKa = 11.84 CKK114 pKa = 10.4 RR115 pKa = 11.84 FCRR118 pKa = 11.84 HH119 pKa = 4.35 QFMWEE124 pKa = 4.12 YY125 pKa = 10.84 NHH127 pKa = 6.54 EE128 pKa = 4.1 LANRR132 pKa = 11.84 GLPAGTGPVAFMLSCRR148 pKa = 11.84 IHH150 pKa = 6.19 PRR152 pKa = 11.84 KK153 pKa = 9.93 LYY155 pKa = 10.3 NPRR158 pKa = 11.84 TVRR161 pKa = 11.84 FGSGRR166 pKa = 11.84 PGQYY170 pKa = 8.51 VAHH173 pKa = 7.75 ILRR176 pKa = 11.84 CPYY179 pKa = 10.05 SDD181 pKa = 3.55 YY182 pKa = 11.36 DD183 pKa = 3.82 VRR185 pKa = 11.84 ISNCTTVQIVTSRR198 pKa = 11.84 GIVYY202 pKa = 9.5 SQNKK206 pKa = 6.48 ATSMEE211 pKa = 4.46 FLNKK215 pKa = 8.6 THH217 pKa = 6.85 LCVPTGEE224 pKa = 3.95 FMQWGGVALEE234 pKa = 4.41 LLRR237 pKa = 11.84 IEE239 pKa = 4.29 PQPVISFTPSPRR251 pKa = 11.84 TRR253 pKa = 11.84 GRR255 pKa = 11.84 FYY257 pKa = 11.21 LVVGVAKK264 pKa = 10.21 AVSSMFSRR272 pKa = 11.84 NPLVRR277 pKa = 11.84 YY278 pKa = 8.61 HH279 pKa = 3.92 VTYY282 pKa = 10.69 RR283 pKa = 11.84 RR284 pKa = 11.84 FASASTFCAIGVAGALMFEE303 pKa = 4.64 LRR305 pKa = 11.84 SIARR309 pKa = 11.84 SLFNMRR315 pKa = 11.84 SQQRR319 pKa = 11.84 FFPMQVEE326 pKa = 4.15 YY327 pKa = 10.97 DD328 pKa = 3.72 VATFAPYY335 pKa = 10.38 NIQLPFEE342 pKa = 4.28 QEE344 pKa = 3.47 LFMRR348 pKa = 11.84 MAVKK352 pKa = 10.31 RR353 pKa = 11.84 DD354 pKa = 3.15 ITDD357 pKa = 2.4 AWIRR361 pKa = 11.84 DD362 pKa = 3.88 VITRR366 pKa = 11.84 LAHH369 pKa = 5.15 QNDD372 pKa = 3.11 WRR374 pKa = 11.84 GVLNRR379 pKa = 11.84 HH380 pKa = 6.27 EE381 pKa = 4.36 LNQWISRR388 pKa = 11.84 VVTEE392 pKa = 5.21 PGLVWEE398 pKa = 4.41 VQKK401 pKa = 11.39 DD402 pKa = 3.78 GLRR405 pKa = 11.84 NNACYY410 pKa = 9.45 TCLSIGRR417 pKa = 11.84 KK418 pKa = 6.85 MRR420 pKa = 11.84 RR421 pKa = 11.84 NEE423 pKa = 3.82 CAQCRR428 pKa = 11.84 TQRR431 pKa = 11.84 NLSPLYY437 pKa = 7.53 FTPIPYY443 pKa = 9.27 TPFAHH448 pKa = 6.11 VGLVGIYY455 pKa = 9.84 SRR457 pKa = 11.84 PPLLPPATYY466 pKa = 10.23 YY467 pKa = 10.53 HH468 pKa = 6.24 SCEE471 pKa = 4.06 EE472 pKa = 4.08 RR473 pKa = 11.84 YY474 pKa = 10.11 LPYY477 pKa = 10.44 KK478 pKa = 10.22 SGGWYY483 pKa = 9.29 PGRR486 pKa = 11.84 PFEE489 pKa = 5.16 FIYY492 pKa = 10.48 HH493 pKa = 6.59 CSYY496 pKa = 10.15 KK497 pKa = 10.82 GKK499 pKa = 10.11 IVNDD503 pKa = 3.87 PEE505 pKa = 3.97 TAYY508 pKa = 10.45 AIYY511 pKa = 10.69 NSFGPQPRR519 pKa = 11.84 CRR521 pKa = 11.84 GKK523 pKa = 10.81 LCGPMLAGAHH533 pKa = 5.17 MQCFTSGTEE542 pKa = 3.83 TALLAFVGRR551 pKa = 11.84 MGAVSPEE558 pKa = 3.92 YY559 pKa = 10.34 QHH561 pKa = 6.42 YY562 pKa = 10.78 LSLGVNHH569 pKa = 6.35 SVYY572 pKa = 11.23 NDD574 pKa = 3.16 IAKK577 pKa = 10.47 VNARR581 pKa = 11.84 HH582 pKa = 6.02 KK583 pKa = 10.55 FDD585 pKa = 3.73 YY586 pKa = 10.74 LLEE589 pKa = 4.34 IFKK592 pKa = 10.86 HH593 pKa = 5.27 VGAAAYY599 pKa = 10.72 YY600 pKa = 10.82 DD601 pKa = 3.87 GDD603 pKa = 3.77 NDD605 pKa = 4.71 AGGLRR610 pKa = 11.84 SKK612 pKa = 10.1 YY613 pKa = 8.72 IPSRR617 pKa = 11.84 EE618 pKa = 4.07 VVPWTPQQVIDD629 pKa = 4.54 HH630 pKa = 6.31 QPDD633 pKa = 3.33 SAKK636 pKa = 10.17 RR637 pKa = 11.84 RR638 pKa = 11.84 IMIEE642 pKa = 3.83 AYY644 pKa = 10.21 RR645 pKa = 11.84 DD646 pKa = 3.15 IDD648 pKa = 3.92 EE649 pKa = 5.48 GFLTPTPKK657 pKa = 10.4 LYY659 pKa = 10.06 KK660 pKa = 10.34 AKK662 pKa = 10.57 AIAKK666 pKa = 9.26 GEE668 pKa = 3.82 KK669 pKa = 10.09 SMAWDD674 pKa = 3.5 HH675 pKa = 7.15 DD676 pKa = 3.58 ISEE679 pKa = 4.27 TLIYY683 pKa = 10.17 KK684 pKa = 10.02 SKK686 pKa = 10.2 QVPRR690 pKa = 11.84 FISACSPHH698 pKa = 6.34 TNALIAPYY706 pKa = 9.57 IAAAEE711 pKa = 4.12 EE712 pKa = 4.37 FVKK715 pKa = 10.22 EE716 pKa = 4.36 FCSSSASVAYY726 pKa = 10.09 ASGMNPEE733 pKa = 4.91 QLNEE737 pKa = 3.96 WLNTYY742 pKa = 10.55 AVDD745 pKa = 3.74 RR746 pKa = 11.84 LIIEE750 pKa = 4.61 GDD752 pKa = 3.12 ISSADD757 pKa = 3.39 SAIGVEE763 pKa = 3.79 AHH765 pKa = 6.31 YY766 pKa = 11.04 LFFYY770 pKa = 10.6 FLTWLFPEE778 pKa = 4.95 LPEE781 pKa = 4.97 SDD783 pKa = 3.37 VWHH786 pKa = 6.63 VIQALSYY793 pKa = 10.4 IYY795 pKa = 10.66 VDD797 pKa = 3.27 RR798 pKa = 11.84 DD799 pKa = 3.03 GSKK802 pKa = 10.49 FVAGPVNVSGSRR814 pKa = 11.84 YY815 pKa = 6.98 TSCIVTIWVMVANVTSSAFSCLSDD839 pKa = 3.83 YY840 pKa = 9.25 RR841 pKa = 11.84 TLSQADD847 pKa = 4.29 RR848 pKa = 11.84 IGMVKK853 pKa = 10.5 GLLQSGNAATITCSDD868 pKa = 4.69 DD869 pKa = 3.0 IYY871 pKa = 10.68 TAVNTSFSEE880 pKa = 3.75 WHH882 pKa = 5.7 FMRR885 pKa = 11.84 NISPYY890 pKa = 9.89 LRR892 pKa = 11.84 AYY894 pKa = 9.37 TYY896 pKa = 9.92 EE897 pKa = 4.42 CSVDD901 pKa = 3.93 SAWLRR906 pKa = 11.84 RR907 pKa = 11.84 FCDD910 pKa = 3.28 TMRR913 pKa = 11.84 TEE915 pKa = 4.37 FFLDD919 pKa = 3.26 VRR921 pKa = 11.84 PNKK924 pKa = 9.89 CRR926 pKa = 11.84 LFPAAEE932 pKa = 3.64 WRR934 pKa = 11.84 LATFLAMRR942 pKa = 11.84 PVWSGSRR949 pKa = 11.84 YY950 pKa = 7.5 EE951 pKa = 4.34 WGPEE955 pKa = 2.86 ICRR958 pKa = 11.84 RR959 pKa = 11.84 MRR961 pKa = 11.84 SAYY964 pKa = 8.71 WMFDD968 pKa = 3.15 KK969 pKa = 10.48 EE970 pKa = 4.7 HH971 pKa = 7.14 PPMAWGRR978 pKa = 11.84 GISAALLVAGRR989 pKa = 11.84 HH990 pKa = 4.53 VPVLRR995 pKa = 11.84 DD996 pKa = 2.77 ICTWHH1001 pKa = 6.98 LSVTHH1006 pKa = 6.07 GPIMKK1011 pKa = 10.36 LPFTNIWSTFYY1022 pKa = 10.32 RR1023 pKa = 11.84 YY1024 pKa = 9.01 EE1025 pKa = 3.91 VSGEE1029 pKa = 3.89 FTDD1032 pKa = 4.31 RR1033 pKa = 11.84 GLNEE1037 pKa = 3.96 FLADD1041 pKa = 3.8 YY1042 pKa = 10.36 DD1043 pKa = 3.82 ISTSEE1048 pKa = 4.0 YY1049 pKa = 10.66 LDD1051 pKa = 4.09 FVDD1054 pKa = 5.38 KK1055 pKa = 11.3 LKK1057 pKa = 11.38 GCMDD1061 pKa = 4.27 PFINIQHH1068 pKa = 5.86 SVIQKK1073 pKa = 10.15 LLMRR1077 pKa = 11.84 EE1078 pKa = 4.05
Molecular weight: 122.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.857
IPC2_protein 7.951
IPC_protein 7.702
Toseland 7.278
ProMoST 8.302
Dawson 8.404
Bjellqvist 8.829
Wikipedia 8.346
Rodwell 8.434
Grimsley 7.322
Solomon 8.434
Lehninger 8.448
Nozaki 8.946
DTASelect 8.521
Thurlkill 8.551
EMBOSS 8.595
Sillero 8.887
Patrickios 3.732
IPC_peptide 8.434
IPC2_peptide 8.039
IPC2.peptide.svr19 8.175
Protein with the highest isoelectric point:
>tr|A0A1L3KL64|A0A1L3KL64_9VIRU RNA-dependent RNA polymerase OS=Wenzhou yanvirus-like virus 1 OX=1923683 PE=4 SV=1
MM1 pKa = 6.33 QQKK4 pKa = 9.0 TSILPSTHH12 pKa = 6.65 PVRR15 pKa = 11.84 SLAGLARR22 pKa = 11.84 QIALPHH28 pKa = 6.06 EE29 pKa = 4.76 FAPEE33 pKa = 4.18 RR34 pKa = 11.84 FPSFPALEE42 pKa = 4.15 RR43 pKa = 11.84 TAVMGFNVPSTWDD56 pKa = 3.48 LPAASTVKK64 pKa = 10.78 AILTRR69 pKa = 11.84 QATFPFWFEE78 pKa = 3.94 KK79 pKa = 9.28 STSGATFRR87 pKa = 11.84 VTWRR91 pKa = 11.84 FPRR94 pKa = 11.84 APGSNTTAYY103 pKa = 10.76 DD104 pKa = 3.58 PVINAAGTGNVAQTINQVGVTNGNNGGIASYY135 pKa = 9.68 PILGLDD141 pKa = 4.25 GMGTCPYY148 pKa = 10.09 MYY150 pKa = 10.4 IPDD153 pKa = 3.61 NWTALMVVSLDD164 pKa = 3.62 VAPATAIDD172 pKa = 3.69 CTVIYY177 pKa = 9.77 EE178 pKa = 4.43 RR179 pKa = 11.84 WNSPGQASVNSVVAGSTIAGNAGCSIPISMPSSEE213 pKa = 4.45 STWIRR218 pKa = 11.84 IVSVSLSTSTAIALGATNVTVVASSGTLAYY248 pKa = 9.42 TPGVLAGNVQVTPAATVALLPGAYY272 pKa = 8.71 PVEE275 pKa = 4.55 FANSQLPWYY284 pKa = 7.41 STRR287 pKa = 11.84 TTAAAVLCTNVSQVLNKK304 pKa = 10.5 GGTVLAGRR312 pKa = 11.84 IAPQVVNPWNVSEE325 pKa = 4.3 TYY327 pKa = 10.65 INGLHH332 pKa = 6.3 PAEE335 pKa = 4.6 KK336 pKa = 10.49 AFLPLEE342 pKa = 4.39 TGLYY346 pKa = 9.76 SYY348 pKa = 10.26 CPPSTDD354 pKa = 5.79 LVDD357 pKa = 4.2 FWDD360 pKa = 3.8 YY361 pKa = 10.69 TLNSTPFFTLPATPLYY377 pKa = 10.68 RR378 pKa = 11.84 LDD380 pKa = 3.46 NTSLVNVMFVTASAVAEE397 pKa = 4.11 TLAVNVDD404 pKa = 2.69 WHH406 pKa = 7.65 IEE408 pKa = 3.85 FRR410 pKa = 11.84 TSSSLFPIGLSAVTLEE426 pKa = 4.51 TLHH429 pKa = 5.81 QAQLALVSAGFFFEE443 pKa = 4.42 NPEE446 pKa = 4.16 HH447 pKa = 6.84 KK448 pKa = 10.32 SVLSKK453 pKa = 11.45 VMTAVKK459 pKa = 10.12 RR460 pKa = 11.84 YY461 pKa = 9.92 GPTALGAVNPAAGRR475 pKa = 11.84 VARR478 pKa = 11.84 TMIQLSSKK486 pKa = 9.2 PASRR490 pKa = 11.84 MKK492 pKa = 9.27 PTSASASGFNGNKK505 pKa = 9.6 KK506 pKa = 9.89 PPRR509 pKa = 11.84 PTPKK513 pKa = 9.87 KK514 pKa = 10.61 GKK516 pKa = 8.61 ATSGKK521 pKa = 9.64 KK522 pKa = 9.85 KK523 pKa = 10.29 KK524 pKa = 10.41
Molecular weight: 55.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.838
IPC2_protein 8.873
IPC_protein 8.799
Toseland 9.326
ProMoST 9.268
Dawson 9.663
Bjellqvist 9.443
Wikipedia 9.853
Rodwell 9.853
Grimsley 9.75
Solomon 9.706
Lehninger 9.648
Nozaki 9.502
DTASelect 9.399
Thurlkill 9.502
EMBOSS 9.794
Sillero 9.633
Patrickios 4.66
IPC_peptide 9.692
IPC2_peptide 8.2
IPC2.peptide.svr19 7.803
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1602
524
1078
801.0
89.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.176 ± 1.481
2.31 ± 0.756
3.62 ± 0.848
3.87 ± 0.562
4.744 ± 0.411
6.492 ± 0.211
2.31 ± 0.649
5.056 ± 0.585
3.371 ± 0.142
7.553 ± 0.151
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.559 ± 0.363
4.057 ± 0.717
6.866 ± 0.747
2.871 ± 0.111
6.117 ± 1.389
8.052 ± 0.724
6.617 ± 1.95
7.615 ± 0.755
2.185 ± 0.154
4.557 ± 1.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here