Changjiang narna-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KIH4|A0A1L3KIH4_9VIRU Capsid protein OS=Changjiang narna-like virus 2 OX=1922777 PE=3 SV=1
MM1 pKa = 7.49NSASCTNYY9 pKa = 9.91TVIHH13 pKa = 7.14LIDD16 pKa = 5.13GILIRR21 pKa = 11.84LHH23 pKa = 6.06AVRR26 pKa = 11.84PEE28 pKa = 3.19WKK30 pKa = 9.24YY31 pKa = 9.72RR32 pKa = 11.84HH33 pKa = 5.95GGPRR37 pKa = 11.84QWLYY41 pKa = 10.21VAEE44 pKa = 4.71PKK46 pKa = 10.3TSFEE50 pKa = 4.15VEE52 pKa = 4.03LAQVATTLSDD62 pKa = 3.47PSFPPSEE69 pKa = 4.04EE70 pKa = 3.8RR71 pKa = 11.84VKK73 pKa = 11.17GGGYY77 pKa = 10.01RR78 pKa = 11.84LPDD81 pKa = 3.39GTVTMVPPPPKK92 pKa = 10.13LEE94 pKa = 4.28SGAALPAPKK103 pKa = 9.42PGQVHH108 pKa = 6.7HH109 pKa = 6.49SVDD112 pKa = 3.02WRR114 pKa = 11.84LLHH117 pKa = 6.49ILSGLRR123 pKa = 11.84AVLLVLVDD131 pKa = 3.84SCGSCAQSPDD141 pKa = 3.53FVGNQPWTDD150 pKa = 3.76SIAVIKK156 pKa = 10.67AMRR159 pKa = 11.84SWSEE163 pKa = 3.5EE164 pKa = 3.8DD165 pKa = 3.5FVPNAKK171 pKa = 9.25YY172 pKa = 9.98WKK174 pKa = 8.97DD175 pKa = 3.04WPLARR180 pKa = 11.84LLKK183 pKa = 10.1QQLPNRR189 pKa = 11.84PPSWKK194 pKa = 9.35YY195 pKa = 11.22GPMACLFTGEE205 pKa = 4.25TGRR208 pKa = 11.84YY209 pKa = 5.1WNRR212 pKa = 11.84IATYY216 pKa = 8.21DD217 pKa = 3.37TTRR220 pKa = 11.84PDD222 pKa = 2.88SFVFYY227 pKa = 10.3RR228 pKa = 11.84AAFGLSQSKK237 pKa = 10.45RR238 pKa = 11.84GFAAVPRR245 pKa = 11.84SFVKK249 pKa = 10.68RR250 pKa = 11.84SLEE253 pKa = 3.6KK254 pKa = 10.2HH255 pKa = 5.71ARR257 pKa = 11.84QLSTPPTSDD266 pKa = 3.58PDD268 pKa = 3.62MEE270 pKa = 4.43SARR273 pKa = 11.84IFAEE277 pKa = 4.11TFFEE281 pKa = 4.7GFRR284 pKa = 11.84IPDD287 pKa = 3.59ILKK290 pKa = 10.15SLPDD294 pKa = 3.23IEE296 pKa = 4.64GTLRR300 pKa = 11.84ACVEE304 pKa = 4.27NPRR307 pKa = 11.84HH308 pKa = 5.81AGGARR313 pKa = 11.84EE314 pKa = 4.26FLRR317 pKa = 11.84HH318 pKa = 6.39LARR321 pKa = 11.84KK322 pKa = 7.74HH323 pKa = 6.13HH324 pKa = 6.19GMLDD328 pKa = 4.03SYY330 pKa = 10.09PVSYY334 pKa = 10.75SGLASVLEE342 pKa = 4.4EE343 pKa = 4.22TPEE346 pKa = 4.0ALPVYY351 pKa = 10.24VKK353 pKa = 9.97TRR355 pKa = 11.84EE356 pKa = 3.88PLVRR360 pKa = 11.84MIEE363 pKa = 3.85ISPGVIVEE371 pKa = 4.06EE372 pKa = 4.73HH373 pKa = 6.51GLPPLSPEE381 pKa = 3.81DD382 pKa = 3.23WRR384 pKa = 11.84VLVNNHH390 pKa = 5.5SPQNARR396 pKa = 11.84EE397 pKa = 3.91YY398 pKa = 10.65LPPGVQDD405 pKa = 3.57AVDD408 pKa = 3.73RR409 pKa = 11.84LIDD412 pKa = 3.89SEE414 pKa = 4.53PEE416 pKa = 3.57ATNFPTCKK424 pKa = 9.5VAEE427 pKa = 4.19VLEE430 pKa = 4.12PLKK433 pKa = 11.07VRR435 pKa = 11.84LITAMDD441 pKa = 4.33ALRR444 pKa = 11.84THH446 pKa = 6.48VARR449 pKa = 11.84PLQAALWRR457 pKa = 11.84YY458 pKa = 9.39LRR460 pKa = 11.84ASPVFALIGEE470 pKa = 5.01PISEE474 pKa = 5.31DD475 pKa = 3.76LLQDD479 pKa = 3.58LCARR483 pKa = 11.84HH484 pKa = 6.04LADD487 pKa = 5.02GGGSNDD493 pKa = 2.88PWASGDD499 pKa = 3.57YY500 pKa = 10.61SAATDD505 pKa = 3.71GLDD508 pKa = 3.09IRR510 pKa = 11.84FSRR513 pKa = 11.84LVIEE517 pKa = 4.52VVLSKK522 pKa = 10.93LRR524 pKa = 11.84TEE526 pKa = 4.98DD527 pKa = 2.75KK528 pKa = 10.34WLAPFLASILDD539 pKa = 3.82QQIITYY545 pKa = 7.31PAWAKK550 pKa = 10.72LPAILQKK557 pKa = 10.7NGQLMGSVLSFPVLCLANFFAFIQSRR583 pKa = 11.84PNAAAILRR591 pKa = 11.84SRR593 pKa = 11.84RR594 pKa = 11.84LMDD597 pKa = 3.84RR598 pKa = 11.84QPVLINGDD606 pKa = 4.26DD607 pKa = 3.35ILFRR611 pKa = 11.84ATEE614 pKa = 3.79EE615 pKa = 4.34RR616 pKa = 11.84YY617 pKa = 9.78KK618 pKa = 11.06VWLSEE623 pKa = 3.74IAKK626 pKa = 10.08VGFVPSVGKK635 pKa = 10.26NFFHH639 pKa = 7.28PRR641 pKa = 11.84FFTVNSVPIEE651 pKa = 4.07YY652 pKa = 10.23LPSPTPYY659 pKa = 10.4QFWSQFSWADD669 pKa = 3.47MEE671 pKa = 5.33EE672 pKa = 4.83IAAPWNINQAPRR684 pKa = 11.84ISIRR688 pKa = 11.84GFLNVGLLTGQAKK701 pKa = 8.73LTGRR705 pKa = 11.84EE706 pKa = 4.0ALGALPLAGWHH717 pKa = 6.38AGSVLEE723 pKa = 5.24ALNPLQAHH731 pKa = 6.53KK732 pKa = 10.41WFLHH736 pKa = 4.24YY737 pKa = 10.31HH738 pKa = 5.73LEE740 pKa = 4.45EE741 pKa = 4.03IQRR744 pKa = 11.84QTRR747 pKa = 11.84FGSTTLNIFAHH758 pKa = 6.58PLLGGLGFQIPVGVEE773 pKa = 3.6PRR775 pKa = 11.84FSPEE779 pKa = 3.14QRR781 pKa = 11.84RR782 pKa = 11.84IARR785 pKa = 11.84ALFLSASASYY795 pKa = 10.1EE796 pKa = 3.93GQEE799 pKa = 4.64SRR801 pKa = 11.84FEE803 pKa = 4.3LDD805 pKa = 3.02SLLFLEE811 pKa = 5.08SRR813 pKa = 11.84TAAPLSSLGNRR824 pKa = 11.84RR825 pKa = 11.84RR826 pKa = 11.84RR827 pKa = 11.84VEE829 pKa = 3.89VEE831 pKa = 4.25LYY833 pKa = 7.73PTGTPLPEE841 pKa = 5.15GYY843 pKa = 10.44SPFTDD848 pKa = 3.32STGVQPLAMVHH859 pKa = 6.57DD860 pKa = 5.28LPSSEE865 pKa = 5.77DD866 pKa = 3.84DD867 pKa = 3.85LPTIARR873 pKa = 11.84CRR875 pKa = 11.84LSSRR879 pKa = 11.84RR880 pKa = 11.84IRR882 pKa = 11.84QLIHH886 pKa = 6.93RR887 pKa = 11.84FDD889 pKa = 4.09DD890 pKa = 4.1TVDD893 pKa = 3.63LHH895 pKa = 6.58PLEE898 pKa = 5.8LMTQFPYY905 pKa = 10.49TPVRR909 pKa = 11.84VTRR912 pKa = 11.84SEE914 pKa = 4.43LIADD918 pKa = 3.78KK919 pKa = 10.96GVVIPEE925 pKa = 3.84EE926 pKa = 4.24RR927 pKa = 11.84PFSKK931 pKa = 10.24VYY933 pKa = 10.86SMEE936 pKa = 4.22IPFQDD941 pKa = 3.5VPVPEE946 pKa = 4.46AAEE949 pKa = 4.16EE950 pKa = 3.99PVLPKK955 pKa = 10.49VYY957 pKa = 10.23EE958 pKa = 4.23PEE960 pKa = 3.89DD961 pKa = 3.52WEE963 pKa = 4.38TATVSLRR970 pKa = 11.84VINPIVAPYY979 pKa = 9.93VPPPLSPEE987 pKa = 3.34EE988 pKa = 3.92RR989 pKa = 11.84YY990 pKa = 10.21RR991 pKa = 11.84RR992 pKa = 11.84MEE994 pKa = 3.5GRR996 pKa = 11.84RR997 pKa = 11.84RR998 pKa = 11.84QVLSRR1003 pKa = 11.84EE1004 pKa = 3.8QNARR1008 pKa = 11.84NRR1010 pKa = 11.84GMIYY1014 pKa = 10.29ARR1016 pKa = 11.84SALEE1020 pKa = 3.42QSYY1023 pKa = 10.36FEE1025 pKa = 4.23II1026 pKa = 6.04

Molecular weight:
115.47 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KIH4|A0A1L3KIH4_9VIRU Capsid protein OS=Changjiang narna-like virus 2 OX=1922777 PE=3 SV=1
MM1 pKa = 7.54PRR3 pKa = 11.84DD4 pKa = 3.65CTALSDD10 pKa = 3.79HH11 pKa = 6.63SALSDD16 pKa = 3.7VQSVPSGWIQYY27 pKa = 7.09TDD29 pKa = 3.33KK30 pKa = 11.43PKK32 pKa = 8.14MTKK35 pKa = 10.2RR36 pKa = 11.84NASSSRR42 pKa = 11.84ARR44 pKa = 11.84SANNRR49 pKa = 11.84TRR51 pKa = 11.84RR52 pKa = 11.84LRR54 pKa = 11.84GKK56 pKa = 10.38GDD58 pKa = 3.35YY59 pKa = 11.02SEE61 pKa = 6.01DD62 pKa = 3.01IRR64 pKa = 11.84NEE66 pKa = 4.02TNVATRR72 pKa = 11.84LEE74 pKa = 4.15KK75 pKa = 10.74KK76 pKa = 9.5IDD78 pKa = 3.36HH79 pKa = 6.75LEE81 pKa = 4.02RR82 pKa = 11.84SLVHH86 pKa = 6.05ATPSIAKK93 pKa = 8.83GASIAGRR100 pKa = 11.84ALGSLLGQGDD110 pKa = 4.82LGAMAGEE117 pKa = 4.53GLSKK121 pKa = 10.99LFGFGDD127 pKa = 3.4YY128 pKa = 10.16RR129 pKa = 11.84VAVKK133 pKa = 10.6GNSLMAGTTSTPVPKK148 pKa = 10.58FSGDD152 pKa = 2.98GRR154 pKa = 11.84RR155 pKa = 11.84GTRR158 pKa = 11.84ITEE161 pKa = 3.82RR162 pKa = 11.84EE163 pKa = 4.03FIGNVLTGSVVSGSSVFNNAVYY185 pKa = 9.53PVNPIDD191 pKa = 4.76SNTFPWLSRR200 pKa = 11.84IAQQFDD206 pKa = 2.58QWEE209 pKa = 3.84PHH211 pKa = 6.41GIVFEE216 pKa = 4.13YY217 pKa = 9.2RR218 pKa = 11.84TTSSTFNGTSQALGAVIMATDD239 pKa = 4.14YY240 pKa = 11.69DD241 pKa = 4.04SLDD244 pKa = 3.04AAFRR248 pKa = 11.84DD249 pKa = 3.81KK250 pKa = 11.18QQMEE254 pKa = 4.24NADD257 pKa = 4.09YY258 pKa = 11.14ACSTVPSQSLLHH270 pKa = 6.58GLEE273 pKa = 4.17CDD275 pKa = 3.3PRR277 pKa = 11.84EE278 pKa = 4.11RR279 pKa = 11.84PLEE282 pKa = 3.91VMYY285 pKa = 9.39TQTRR289 pKa = 11.84AGNQNFSSLGNFQIATQGCSTAGTTLGEE317 pKa = 3.9LWISYY322 pKa = 10.48DD323 pKa = 2.69ITFYY327 pKa = 11.32KK328 pKa = 10.28KK329 pKa = 10.19VLPEE333 pKa = 4.11HH334 pKa = 6.74SEE336 pKa = 3.78SSPFVNINNKK346 pKa = 9.97AIALSQLFKK355 pKa = 10.81PNSATIAKK363 pKa = 9.39DD364 pKa = 2.85ITVALIPGYY373 pKa = 7.34GTRR376 pKa = 11.84FVFPPSQSSGRR387 pKa = 11.84YY388 pKa = 9.02LFLAYY393 pKa = 9.68MSEE396 pKa = 4.22FLSGDD401 pKa = 3.45LGQIVPFGHH410 pKa = 6.67KK411 pKa = 9.96NCEE414 pKa = 4.08QVSGYY419 pKa = 11.09AGTNTGIGTPGVSSSVINITGPGAWFHH446 pKa = 6.46TGLKK450 pKa = 10.08QGTPPQEE457 pKa = 4.55ANAKK461 pKa = 9.11MILIPIDD468 pKa = 4.79FDD470 pKa = 4.52TNWDD474 pKa = 3.44VTPPVV479 pKa = 3.38

Molecular weight:
51.82 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1505

479

1026

752.5

83.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.106 ± 0.203

0.93 ± 0.06

4.585 ± 0.227

5.98 ± 1.072

4.518 ± 0.04

6.777 ± 1.393

2.126 ± 0.243

4.983 ± 0.237

3.256 ± 0.378

9.568 ± 1.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.661 ± 0.116

3.455 ± 0.939

7.973 ± 1.244

3.721 ± 0.242

7.176 ± 1.041

8.372 ± 0.989

5.581 ± 1.252

6.512 ± 0.466

1.794 ± 0.288

2.924 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski