Changjiang narna-like virus 2
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KIH4|A0A1L3KIH4_9VIRU Capsid protein OS=Changjiang narna-like virus 2 OX=1922777 PE=3 SV=1
MM1 pKa = 7.49 NSASCTNYY9 pKa = 9.91 TVIHH13 pKa = 7.14 LIDD16 pKa = 5.13 GILIRR21 pKa = 11.84 LHH23 pKa = 6.06 AVRR26 pKa = 11.84 PEE28 pKa = 3.19 WKK30 pKa = 9.24 YY31 pKa = 9.72 RR32 pKa = 11.84 HH33 pKa = 5.95 GGPRR37 pKa = 11.84 QWLYY41 pKa = 10.21 VAEE44 pKa = 4.71 PKK46 pKa = 10.3 TSFEE50 pKa = 4.15 VEE52 pKa = 4.03 LAQVATTLSDD62 pKa = 3.47 PSFPPSEE69 pKa = 4.04 EE70 pKa = 3.8 RR71 pKa = 11.84 VKK73 pKa = 11.17 GGGYY77 pKa = 10.01 RR78 pKa = 11.84 LPDD81 pKa = 3.39 GTVTMVPPPPKK92 pKa = 10.13 LEE94 pKa = 4.28 SGAALPAPKK103 pKa = 9.42 PGQVHH108 pKa = 6.7 HH109 pKa = 6.49 SVDD112 pKa = 3.02 WRR114 pKa = 11.84 LLHH117 pKa = 6.49 ILSGLRR123 pKa = 11.84 AVLLVLVDD131 pKa = 3.84 SCGSCAQSPDD141 pKa = 3.53 FVGNQPWTDD150 pKa = 3.76 SIAVIKK156 pKa = 10.67 AMRR159 pKa = 11.84 SWSEE163 pKa = 3.5 EE164 pKa = 3.8 DD165 pKa = 3.5 FVPNAKK171 pKa = 9.25 YY172 pKa = 9.98 WKK174 pKa = 8.97 DD175 pKa = 3.04 WPLARR180 pKa = 11.84 LLKK183 pKa = 10.1 QQLPNRR189 pKa = 11.84 PPSWKK194 pKa = 9.35 YY195 pKa = 11.22 GPMACLFTGEE205 pKa = 4.25 TGRR208 pKa = 11.84 YY209 pKa = 5.1 WNRR212 pKa = 11.84 IATYY216 pKa = 8.21 DD217 pKa = 3.37 TTRR220 pKa = 11.84 PDD222 pKa = 2.88 SFVFYY227 pKa = 10.3 RR228 pKa = 11.84 AAFGLSQSKK237 pKa = 10.45 RR238 pKa = 11.84 GFAAVPRR245 pKa = 11.84 SFVKK249 pKa = 10.68 RR250 pKa = 11.84 SLEE253 pKa = 3.6 KK254 pKa = 10.2 HH255 pKa = 5.71 ARR257 pKa = 11.84 QLSTPPTSDD266 pKa = 3.58 PDD268 pKa = 3.62 MEE270 pKa = 4.43 SARR273 pKa = 11.84 IFAEE277 pKa = 4.11 TFFEE281 pKa = 4.7 GFRR284 pKa = 11.84 IPDD287 pKa = 3.59 ILKK290 pKa = 10.15 SLPDD294 pKa = 3.23 IEE296 pKa = 4.64 GTLRR300 pKa = 11.84 ACVEE304 pKa = 4.27 NPRR307 pKa = 11.84 HH308 pKa = 5.81 AGGARR313 pKa = 11.84 EE314 pKa = 4.26 FLRR317 pKa = 11.84 HH318 pKa = 6.39 LARR321 pKa = 11.84 KK322 pKa = 7.74 HH323 pKa = 6.13 HH324 pKa = 6.19 GMLDD328 pKa = 4.03 SYY330 pKa = 10.09 PVSYY334 pKa = 10.75 SGLASVLEE342 pKa = 4.4 EE343 pKa = 4.22 TPEE346 pKa = 4.0 ALPVYY351 pKa = 10.24 VKK353 pKa = 9.97 TRR355 pKa = 11.84 EE356 pKa = 3.88 PLVRR360 pKa = 11.84 MIEE363 pKa = 3.85 ISPGVIVEE371 pKa = 4.06 EE372 pKa = 4.73 HH373 pKa = 6.51 GLPPLSPEE381 pKa = 3.81 DD382 pKa = 3.23 WRR384 pKa = 11.84 VLVNNHH390 pKa = 5.5 SPQNARR396 pKa = 11.84 EE397 pKa = 3.91 YY398 pKa = 10.65 LPPGVQDD405 pKa = 3.57 AVDD408 pKa = 3.73 RR409 pKa = 11.84 LIDD412 pKa = 3.89 SEE414 pKa = 4.53 PEE416 pKa = 3.57 ATNFPTCKK424 pKa = 9.5 VAEE427 pKa = 4.19 VLEE430 pKa = 4.12 PLKK433 pKa = 11.07 VRR435 pKa = 11.84 LITAMDD441 pKa = 4.33 ALRR444 pKa = 11.84 THH446 pKa = 6.48 VARR449 pKa = 11.84 PLQAALWRR457 pKa = 11.84 YY458 pKa = 9.39 LRR460 pKa = 11.84 ASPVFALIGEE470 pKa = 5.01 PISEE474 pKa = 5.31 DD475 pKa = 3.76 LLQDD479 pKa = 3.58 LCARR483 pKa = 11.84 HH484 pKa = 6.04 LADD487 pKa = 5.02 GGGSNDD493 pKa = 2.88 PWASGDD499 pKa = 3.57 YY500 pKa = 10.61 SAATDD505 pKa = 3.71 GLDD508 pKa = 3.09 IRR510 pKa = 11.84 FSRR513 pKa = 11.84 LVIEE517 pKa = 4.52 VVLSKK522 pKa = 10.93 LRR524 pKa = 11.84 TEE526 pKa = 4.98 DD527 pKa = 2.75 KK528 pKa = 10.34 WLAPFLASILDD539 pKa = 3.82 QQIITYY545 pKa = 7.31 PAWAKK550 pKa = 10.72 LPAILQKK557 pKa = 10.7 NGQLMGSVLSFPVLCLANFFAFIQSRR583 pKa = 11.84 PNAAAILRR591 pKa = 11.84 SRR593 pKa = 11.84 RR594 pKa = 11.84 LMDD597 pKa = 3.84 RR598 pKa = 11.84 QPVLINGDD606 pKa = 4.26 DD607 pKa = 3.35 ILFRR611 pKa = 11.84 ATEE614 pKa = 3.79 EE615 pKa = 4.34 RR616 pKa = 11.84 YY617 pKa = 9.78 KK618 pKa = 11.06 VWLSEE623 pKa = 3.74 IAKK626 pKa = 10.08 VGFVPSVGKK635 pKa = 10.26 NFFHH639 pKa = 7.28 PRR641 pKa = 11.84 FFTVNSVPIEE651 pKa = 4.07 YY652 pKa = 10.23 LPSPTPYY659 pKa = 10.4 QFWSQFSWADD669 pKa = 3.47 MEE671 pKa = 5.33 EE672 pKa = 4.83 IAAPWNINQAPRR684 pKa = 11.84 ISIRR688 pKa = 11.84 GFLNVGLLTGQAKK701 pKa = 8.73 LTGRR705 pKa = 11.84 EE706 pKa = 4.0 ALGALPLAGWHH717 pKa = 6.38 AGSVLEE723 pKa = 5.24 ALNPLQAHH731 pKa = 6.53 KK732 pKa = 10.41 WFLHH736 pKa = 4.24 YY737 pKa = 10.31 HH738 pKa = 5.73 LEE740 pKa = 4.45 EE741 pKa = 4.03 IQRR744 pKa = 11.84 QTRR747 pKa = 11.84 FGSTTLNIFAHH758 pKa = 6.58 PLLGGLGFQIPVGVEE773 pKa = 3.6 PRR775 pKa = 11.84 FSPEE779 pKa = 3.14 QRR781 pKa = 11.84 RR782 pKa = 11.84 IARR785 pKa = 11.84 ALFLSASASYY795 pKa = 10.1 EE796 pKa = 3.93 GQEE799 pKa = 4.64 SRR801 pKa = 11.84 FEE803 pKa = 4.3 LDD805 pKa = 3.02 SLLFLEE811 pKa = 5.08 SRR813 pKa = 11.84 TAAPLSSLGNRR824 pKa = 11.84 RR825 pKa = 11.84 RR826 pKa = 11.84 RR827 pKa = 11.84 VEE829 pKa = 3.89 VEE831 pKa = 4.25 LYY833 pKa = 7.73 PTGTPLPEE841 pKa = 5.15 GYY843 pKa = 10.44 SPFTDD848 pKa = 3.32 STGVQPLAMVHH859 pKa = 6.57 DD860 pKa = 5.28 LPSSEE865 pKa = 5.77 DD866 pKa = 3.84 DD867 pKa = 3.85 LPTIARR873 pKa = 11.84 CRR875 pKa = 11.84 LSSRR879 pKa = 11.84 RR880 pKa = 11.84 IRR882 pKa = 11.84 QLIHH886 pKa = 6.93 RR887 pKa = 11.84 FDD889 pKa = 4.09 DD890 pKa = 4.1 TVDD893 pKa = 3.63 LHH895 pKa = 6.58 PLEE898 pKa = 5.8 LMTQFPYY905 pKa = 10.49 TPVRR909 pKa = 11.84 VTRR912 pKa = 11.84 SEE914 pKa = 4.43 LIADD918 pKa = 3.78 KK919 pKa = 10.96 GVVIPEE925 pKa = 3.84 EE926 pKa = 4.24 RR927 pKa = 11.84 PFSKK931 pKa = 10.24 VYY933 pKa = 10.86 SMEE936 pKa = 4.22 IPFQDD941 pKa = 3.5 VPVPEE946 pKa = 4.46 AAEE949 pKa = 4.16 EE950 pKa = 3.99 PVLPKK955 pKa = 10.49 VYY957 pKa = 10.23 EE958 pKa = 4.23 PEE960 pKa = 3.89 DD961 pKa = 3.52 WEE963 pKa = 4.38 TATVSLRR970 pKa = 11.84 VINPIVAPYY979 pKa = 9.93 VPPPLSPEE987 pKa = 3.34 EE988 pKa = 3.92 RR989 pKa = 11.84 YY990 pKa = 10.21 RR991 pKa = 11.84 RR992 pKa = 11.84 MEE994 pKa = 3.5 GRR996 pKa = 11.84 RR997 pKa = 11.84 RR998 pKa = 11.84 QVLSRR1003 pKa = 11.84 EE1004 pKa = 3.8 QNARR1008 pKa = 11.84 NRR1010 pKa = 11.84 GMIYY1014 pKa = 10.29 ARR1016 pKa = 11.84 SALEE1020 pKa = 3.42 QSYY1023 pKa = 10.36 FEE1025 pKa = 4.23 II1026 pKa = 6.04
Molecular weight: 115.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.42
IPC2_protein 6.376
IPC_protein 6.478
Toseland 6.751
ProMoST 6.883
Dawson 6.825
Bjellqvist 6.81
Wikipedia 6.839
Rodwell 6.825
Grimsley 6.898
Solomon 6.839
Lehninger 6.839
Nozaki 7.102
DTASelect 7.249
Thurlkill 7.278
EMBOSS 7.263
Sillero 7.22
Patrickios 4.457
IPC_peptide 6.854
IPC2_peptide 7.015
IPC2.peptide.svr19 6.877
Protein with the highest isoelectric point:
>tr|A0A1L3KIH4|A0A1L3KIH4_9VIRU Capsid protein OS=Changjiang narna-like virus 2 OX=1922777 PE=3 SV=1
MM1 pKa = 7.54 PRR3 pKa = 11.84 DD4 pKa = 3.65 CTALSDD10 pKa = 3.79 HH11 pKa = 6.63 SALSDD16 pKa = 3.7 VQSVPSGWIQYY27 pKa = 7.09 TDD29 pKa = 3.33 KK30 pKa = 11.43 PKK32 pKa = 8.14 MTKK35 pKa = 10.2 RR36 pKa = 11.84 NASSSRR42 pKa = 11.84 ARR44 pKa = 11.84 SANNRR49 pKa = 11.84 TRR51 pKa = 11.84 RR52 pKa = 11.84 LRR54 pKa = 11.84 GKK56 pKa = 10.38 GDD58 pKa = 3.35 YY59 pKa = 11.02 SEE61 pKa = 6.01 DD62 pKa = 3.01 IRR64 pKa = 11.84 NEE66 pKa = 4.02 TNVATRR72 pKa = 11.84 LEE74 pKa = 4.15 KK75 pKa = 10.74 KK76 pKa = 9.5 IDD78 pKa = 3.36 HH79 pKa = 6.75 LEE81 pKa = 4.02 RR82 pKa = 11.84 SLVHH86 pKa = 6.05 ATPSIAKK93 pKa = 8.83 GASIAGRR100 pKa = 11.84 ALGSLLGQGDD110 pKa = 4.82 LGAMAGEE117 pKa = 4.53 GLSKK121 pKa = 10.99 LFGFGDD127 pKa = 3.4 YY128 pKa = 10.16 RR129 pKa = 11.84 VAVKK133 pKa = 10.6 GNSLMAGTTSTPVPKK148 pKa = 10.58 FSGDD152 pKa = 2.98 GRR154 pKa = 11.84 RR155 pKa = 11.84 GTRR158 pKa = 11.84 ITEE161 pKa = 3.82 RR162 pKa = 11.84 EE163 pKa = 4.03 FIGNVLTGSVVSGSSVFNNAVYY185 pKa = 9.53 PVNPIDD191 pKa = 4.76 SNTFPWLSRR200 pKa = 11.84 IAQQFDD206 pKa = 2.58 QWEE209 pKa = 3.84 PHH211 pKa = 6.41 GIVFEE216 pKa = 4.13 YY217 pKa = 9.2 RR218 pKa = 11.84 TTSSTFNGTSQALGAVIMATDD239 pKa = 4.14 YY240 pKa = 11.69 DD241 pKa = 4.04 SLDD244 pKa = 3.04 AAFRR248 pKa = 11.84 DD249 pKa = 3.81 KK250 pKa = 11.18 QQMEE254 pKa = 4.24 NADD257 pKa = 4.09 YY258 pKa = 11.14 ACSTVPSQSLLHH270 pKa = 6.58 GLEE273 pKa = 4.17 CDD275 pKa = 3.3 PRR277 pKa = 11.84 EE278 pKa = 4.11 RR279 pKa = 11.84 PLEE282 pKa = 3.91 VMYY285 pKa = 9.39 TQTRR289 pKa = 11.84 AGNQNFSSLGNFQIATQGCSTAGTTLGEE317 pKa = 3.9 LWISYY322 pKa = 10.48 DD323 pKa = 2.69 ITFYY327 pKa = 11.32 KK328 pKa = 10.28 KK329 pKa = 10.19 VLPEE333 pKa = 4.11 HH334 pKa = 6.74 SEE336 pKa = 3.78 SSPFVNINNKK346 pKa = 9.97 AIALSQLFKK355 pKa = 10.81 PNSATIAKK363 pKa = 9.39 DD364 pKa = 2.85 ITVALIPGYY373 pKa = 7.34 GTRR376 pKa = 11.84 FVFPPSQSSGRR387 pKa = 11.84 YY388 pKa = 9.02 LFLAYY393 pKa = 9.68 MSEE396 pKa = 4.22 FLSGDD401 pKa = 3.45 LGQIVPFGHH410 pKa = 6.67 KK411 pKa = 9.96 NCEE414 pKa = 4.08 QVSGYY419 pKa = 11.09 AGTNTGIGTPGVSSSVINITGPGAWFHH446 pKa = 6.46 TGLKK450 pKa = 10.08 QGTPPQEE457 pKa = 4.55 ANAKK461 pKa = 9.11 MILIPIDD468 pKa = 4.79 FDD470 pKa = 4.52 TNWDD474 pKa = 3.44 VTPPVV479 pKa = 3.38
Molecular weight: 51.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.735
IPC2_protein 6.912
IPC_protein 7.044
Toseland 6.927
ProMoST 7.468
Dawson 7.644
Bjellqvist 7.658
Wikipedia 7.585
Rodwell 7.629
Grimsley 7.015
Solomon 7.732
Lehninger 7.746
Nozaki 7.922
DTASelect 7.79
Thurlkill 7.834
EMBOSS 7.907
Sillero 8.053
Patrickios 4.355
IPC_peptide 7.732
IPC2_peptide 7.205
IPC2.peptide.svr19 7.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1505
479
1026
752.5
83.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.106 ± 0.203
0.93 ± 0.06
4.585 ± 0.227
5.98 ± 1.072
4.518 ± 0.04
6.777 ± 1.393
2.126 ± 0.243
4.983 ± 0.237
3.256 ± 0.378
9.568 ± 1.314
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.661 ± 0.116
3.455 ± 0.939
7.973 ± 1.244
3.721 ± 0.242
7.176 ± 1.041
8.372 ± 0.989
5.581 ± 1.252
6.512 ± 0.466
1.794 ± 0.288
2.924 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here