Parabacteroides phage YZ-2015b

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2UMJ3|A0A0G2UMJ3_9VIRU Replication protein VP4 OS=Parabacteroides phage YZ-2015b OX=1655645 PE=4 SV=1
MM1 pKa = 7.29FVTAYY6 pKa = 10.37NFSNLPSEE14 pKa = 5.37LIQEE18 pKa = 4.37KK19 pKa = 10.76VSDD22 pKa = 4.05EE23 pKa = 4.28VLVEE27 pKa = 3.97PSEE30 pKa = 4.45SFTVRR35 pKa = 11.84EE36 pKa = 4.31LIYY39 pKa = 10.12RR40 pKa = 11.84LAMGMPVSSGVRR52 pKa = 11.84SGDD55 pKa = 3.97YY56 pKa = 9.95PDD58 pKa = 5.25HH59 pKa = 7.21DD60 pKa = 3.7QDD62 pKa = 5.03FDD64 pKa = 4.42DD65 pKa = 4.81VLPTEE70 pKa = 5.7DD71 pKa = 4.94PDD73 pKa = 5.31FDD75 pKa = 4.22LADD78 pKa = 3.67YY79 pKa = 7.43ATLKK83 pKa = 10.79NDD85 pKa = 3.32LADD88 pKa = 4.02RR89 pKa = 11.84EE90 pKa = 4.25RR91 pKa = 11.84QRR93 pKa = 11.84KK94 pKa = 8.65IDD96 pKa = 3.4MEE98 pKa = 4.07KK99 pKa = 10.32AYY101 pKa = 9.87KK102 pKa = 10.16EE103 pKa = 3.98KK104 pKa = 10.96LEE106 pKa = 4.16KK107 pKa = 10.71EE108 pKa = 4.27KK109 pKa = 10.55TPDD112 pKa = 3.89PAPDD116 pKa = 3.77PAQQ119 pKa = 3.52

Molecular weight: 13.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2UEI2|A0A0G2UEI2_9VIRU Major capsid protein VP1 OS=Parabacteroides phage YZ-2015b OX=1655645 PE=3 SV=1
MM1 pKa = 7.43QLAKK5 pKa = 10.62YY6 pKa = 8.83QNNWQTAEE14 pKa = 3.97NEE16 pKa = 3.77KK17 pKa = 10.75AYY19 pKa = 10.63ARR21 pKa = 11.84SVEE24 pKa = 4.08MWNMQNQYY32 pKa = 10.84NSPTAQMSRR41 pKa = 11.84LRR43 pKa = 11.84QAGLNPNLVYY53 pKa = 10.91GSGVTGNSAGSAPQYY68 pKa = 10.63QPAKK72 pKa = 8.81IQRR75 pKa = 11.84ATMEE79 pKa = 4.77PYY81 pKa = 10.4RR82 pKa = 11.84GWNLGLSDD90 pKa = 4.06AASMYY95 pKa = 10.14MAMRR99 pKa = 11.84QNKK102 pKa = 8.88AQVEE106 pKa = 4.09NMEE109 pKa = 4.56AQNKK113 pKa = 8.92LIKK116 pKa = 10.05EE117 pKa = 4.03QARR120 pKa = 11.84TEE122 pKa = 4.31GIRR125 pKa = 11.84QGNIAMSTARR135 pKa = 11.84SGFDD139 pKa = 3.37LNLARR144 pKa = 11.84EE145 pKa = 4.28LRR147 pKa = 11.84NVSIDD152 pKa = 3.27RR153 pKa = 11.84AIAEE157 pKa = 4.52KK158 pKa = 10.65NLSEE162 pKa = 4.5ASAAGAWTGANQKK175 pKa = 9.24VLQYY179 pKa = 10.62EE180 pKa = 4.52LDD182 pKa = 3.39RR183 pKa = 11.84TLFDD187 pKa = 4.34NKK189 pKa = 10.19IKK191 pKa = 10.81LSNAQYY197 pKa = 8.23ATAMEE202 pKa = 5.83GIRR205 pKa = 11.84KK206 pKa = 8.3LQQDD210 pKa = 3.1NDD212 pKa = 3.17INAFRR217 pKa = 11.84NRR219 pKa = 11.84MEE221 pKa = 4.37RR222 pKa = 11.84LFGDD226 pKa = 3.27SSDD229 pKa = 3.9AKK231 pKa = 10.68NVASEE236 pKa = 3.99LFKK239 pKa = 11.36RR240 pKa = 11.84MMMYY244 pKa = 9.39MFRR247 pKa = 11.84DD248 pKa = 3.61SRR250 pKa = 11.84EE251 pKa = 3.78LDD253 pKa = 3.01QMFNPKK259 pKa = 9.97

Molecular weight: 29.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1216

119

553

304.0

35.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.319 ± 1.555

1.069 ± 0.739

6.003 ± 1.088

6.908 ± 0.757

4.934 ± 0.739

5.839 ± 1.075

1.727 ± 0.592

3.783 ± 0.265

5.592 ± 1.2

7.484 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

5.016 ± 0.684

6.086 ± 1.402

4.852 ± 0.812

4.605 ± 1.062

6.826 ± 0.5

6.332 ± 1.136

3.947 ± 0.574

5.345 ± 0.92

1.48 ± 0.215

4.852 ± 0.668
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski