Clostridium sp. chh4-2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5111 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K2TUR2|A0A2K2TUR2_9CLOT Exopolyphosphatase OS=Clostridium sp. chh4-2 OX=2067550 GN=C0033_11435 PE=4 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84GMAAALAAMLTIGSALTGCGGGGTGGTTAASTAAPTEE41 pKa = 4.35TAASQAEE48 pKa = 4.34TSVGEE53 pKa = 4.23TNAPAKK59 pKa = 9.08EE60 pKa = 3.99LNYY63 pKa = 10.16ISTFDD68 pKa = 3.3VAGAGDD74 pKa = 3.59VALDD78 pKa = 3.92VMITSLGDD86 pKa = 3.52SDD88 pKa = 3.79GGPYY92 pKa = 10.3LRR94 pKa = 11.84GVMDD98 pKa = 6.0DD99 pKa = 3.81YY100 pKa = 11.32MAMYY104 pKa = 10.05PNVKK108 pKa = 9.85LNPVEE113 pKa = 5.23CSMNDD118 pKa = 3.25LYY120 pKa = 9.27TTLITQATAGTLPDD134 pKa = 3.67IFTMTEE140 pKa = 3.87AYY142 pKa = 9.76SANCLEE148 pKa = 4.71MGMSVDD154 pKa = 5.27NMAEE158 pKa = 4.04LLGDD162 pKa = 3.87EE163 pKa = 4.43YY164 pKa = 11.72LNGLMEE170 pKa = 4.51AAVDD174 pKa = 3.63NATVDD179 pKa = 3.43GTLVYY184 pKa = 9.94MPWQNNITAMVYY196 pKa = 10.28RR197 pKa = 11.84KK198 pKa = 10.27DD199 pKa = 3.38LFEE202 pKa = 4.49EE203 pKa = 4.28KK204 pKa = 10.44GIEE207 pKa = 4.49IPKK210 pKa = 8.75TWDD213 pKa = 3.13EE214 pKa = 4.1FLEE217 pKa = 4.31AAKK220 pKa = 9.63TLTEE224 pKa = 4.84DD225 pKa = 4.36LDD227 pKa = 5.34GDD229 pKa = 4.27GKK231 pKa = 8.89TDD233 pKa = 3.07RR234 pKa = 11.84YY235 pKa = 10.15GAAFAGTRR243 pKa = 11.84NDD245 pKa = 3.38SAEE248 pKa = 4.05SRR250 pKa = 11.84FQTFALTYY258 pKa = 9.76GCDD261 pKa = 3.37FVTDD265 pKa = 3.79NGDD268 pKa = 3.41GTFTSGFGTEE278 pKa = 4.04EE279 pKa = 4.2FKK281 pKa = 11.38NAMTSFVNMAANEE294 pKa = 4.32GVTPPGFIEE303 pKa = 4.06TGYY306 pKa = 10.39SEE308 pKa = 5.71AYY310 pKa = 9.41TMIAADD316 pKa = 3.8QACMFFSASNVLGGIYY332 pKa = 10.02NANPDD337 pKa = 3.74MKK339 pKa = 10.65GKK341 pKa = 9.22MGSFPMPTAEE351 pKa = 4.35GVDD354 pKa = 3.81PVTSFSSVGMTISNTCKK371 pKa = 10.24NPEE374 pKa = 3.79VAADD378 pKa = 3.56FLKK381 pKa = 11.29YY382 pKa = 7.52MTSVDD387 pKa = 5.12NSVTWNQATCRR398 pKa = 11.84LPVVKK403 pKa = 10.38DD404 pKa = 3.35ALTAICEE411 pKa = 3.99ADD413 pKa = 3.18EE414 pKa = 4.96AYY416 pKa = 10.79SGFADD421 pKa = 3.86ASDD424 pKa = 3.68SAVIYY429 pKa = 9.28PAFAGLAEE437 pKa = 4.46LRR439 pKa = 11.84DD440 pKa = 3.8ACGEE444 pKa = 3.99CWQTVVAEE452 pKa = 4.46GASIDD457 pKa = 4.03DD458 pKa = 3.93AVAAAAAKK466 pKa = 10.64AEE468 pKa = 4.38DD469 pKa = 3.75VAKK472 pKa = 10.43SYY474 pKa = 11.59SKK476 pKa = 11.32

Molecular weight: 50.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K2TV41|A0A2K2TV41_9CLOT 1-acyl-sn-glycerol-3-phosphate acyltransferase OS=Clostridium sp. chh4-2 OX=2067550 GN=C0033_11420 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.73MTFQPKK8 pKa = 8.63NRR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 8.89VHH16 pKa = 5.88GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 9.06VIASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.4GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5111

0

5111

1759323

27

12064

344.2

38.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.475 ± 0.04

1.545 ± 0.017

5.551 ± 0.039

7.359 ± 0.04

4.216 ± 0.029

7.36 ± 0.032

1.734 ± 0.017

7.312 ± 0.036

6.383 ± 0.025

9.115 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.284 ± 0.022

4.251 ± 0.026

3.61 ± 0.021

3.212 ± 0.021

4.291 ± 0.026

5.987 ± 0.026

5.257 ± 0.036

6.8 ± 0.03

1.025 ± 0.013

4.233 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski