Salana multivorans
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3332 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N2DBE7|A0A3N2DBE7_9MICO Exoribonuclease R OS=Salana multivorans OX=120377 GN=EDD28_1712 PE=4 SV=1
MM1 pKa = 7.23 RR2 pKa = 11.84 TSSSRR7 pKa = 11.84 VLAAIVALGVCLGLTACVPGLIDD30 pKa = 4.5 PFAGGNGSSTDD41 pKa = 3.66 PSDD44 pKa = 5.55 ADD46 pKa = 3.95 DD47 pKa = 4.11 TTDD50 pKa = 4.56 PDD52 pKa = 4.39 DD53 pKa = 4.87 PSDD56 pKa = 3.53 AAEE59 pKa = 4.46 APVVLDD65 pKa = 4.65 PGPSSTAIDD74 pKa = 3.72 WRR76 pKa = 11.84 PCDD79 pKa = 3.11 HH80 pKa = 7.22 HH81 pKa = 8.52 ARR83 pKa = 11.84 FDD85 pKa = 3.85 CAEE88 pKa = 3.86 VLLPLDD94 pKa = 4.13 HH95 pKa = 7.51 ADD97 pKa = 3.59 PGGDD101 pKa = 3.13 QVRR104 pKa = 11.84 IALRR108 pKa = 11.84 RR109 pKa = 11.84 LPAGGPEE116 pKa = 3.96 EE117 pKa = 4.44 EE118 pKa = 4.49 RR119 pKa = 11.84 LGTLWINPGGPGEE132 pKa = 4.19 SGIDD136 pKa = 3.62 LVTMVGSFFGDD147 pKa = 3.53 DD148 pKa = 3.14 LLEE151 pKa = 5.2 RR152 pKa = 11.84 YY153 pKa = 9.68 DD154 pKa = 3.93 VVGFDD159 pKa = 3.76 PRR161 pKa = 11.84 GVGASTHH168 pKa = 5.09 VVCLLDD174 pKa = 5.63 GEE176 pKa = 5.57 DD177 pKa = 3.78 DD178 pKa = 4.39 PSWVDD183 pKa = 4.98 LDD185 pKa = 4.61 LDD187 pKa = 4.14 TPKK190 pKa = 11.09 GLARR194 pKa = 11.84 VEE196 pKa = 4.48 SGFTALGEE204 pKa = 4.21 LCRR207 pKa = 11.84 EE208 pKa = 3.87 RR209 pKa = 11.84 SGALLDD215 pKa = 4.08 HH216 pKa = 6.92 VSTADD221 pKa = 3.4 TVADD225 pKa = 4.46 LEE227 pKa = 4.31 ALRR230 pKa = 11.84 ALTGEE235 pKa = 3.88 QLNYY239 pKa = 10.54 LGYY242 pKa = 10.64 SYY244 pKa = 10.14 GTLIGAVYY252 pKa = 10.84 ADD254 pKa = 4.56 TYY256 pKa = 9.17 PEE258 pKa = 3.84 RR259 pKa = 11.84 VGRR262 pKa = 11.84 MVLDD266 pKa = 4.0 GAIDD270 pKa = 3.85 PSVGYY275 pKa = 9.06 TDD277 pKa = 5.48 LQAQQTWTFQRR288 pKa = 11.84 AFARR292 pKa = 11.84 FVTTCRR298 pKa = 11.84 EE299 pKa = 4.07 DD300 pKa = 3.56 ADD302 pKa = 4.62 CPLDD306 pKa = 4.12 ADD308 pKa = 3.8 PRR310 pKa = 11.84 AAEE313 pKa = 4.18 TQVLDD318 pKa = 4.47 WIDD321 pKa = 3.92 EE322 pKa = 4.38 LDD324 pKa = 3.85 VSPVPSDD331 pKa = 4.02 AEE333 pKa = 4.4 DD334 pKa = 4.46 GEE336 pKa = 4.58 LTGQEE341 pKa = 4.14 LVAALASALYY351 pKa = 10.34 APWDD355 pKa = 3.4 WDD357 pKa = 3.88 VVVEE361 pKa = 4.39 DD362 pKa = 4.83 LADD365 pKa = 3.61 AVADD369 pKa = 3.89 SDD371 pKa = 4.44 PDD373 pKa = 3.89 GSALDD378 pKa = 3.82 EE379 pKa = 4.98 LQGEE383 pKa = 4.84 WYY385 pKa = 10.34 GGALNYY391 pKa = 9.11 PFWFSAIDD399 pKa = 3.75 CVDD402 pKa = 3.73 YY403 pKa = 10.76 PVGTFDD409 pKa = 4.67 EE410 pKa = 5.0 AVTHH414 pKa = 5.78 ARR416 pKa = 11.84 EE417 pKa = 3.95 LAAGSPVFGAGSIAEE432 pKa = 4.36 IQCATWPAKK441 pKa = 10.62 ARR443 pKa = 11.84 DD444 pKa = 3.55 EE445 pKa = 4.12 RR446 pKa = 11.84 VPVSGTGAAPILVVGTLFDD465 pKa = 4.24 PATPYY470 pKa = 10.95 AWAVSLSRR478 pKa = 11.84 QLEE481 pKa = 4.37 SATLLTYY488 pKa = 10.61 EE489 pKa = 4.56 GDD491 pKa = 3.17 GHH493 pKa = 6.92 AVYY496 pKa = 10.36 GGVSWCVDD504 pKa = 3.32 GFVEE508 pKa = 6.25 DD509 pKa = 4.1 YY510 pKa = 10.88 LIDD513 pKa = 4.13 GAVPPPGMSCPSAFF527 pKa = 3.76
Molecular weight: 55.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.745
IPC_protein 3.795
Toseland 3.554
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.465
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.164
Thurlkill 3.605
EMBOSS 3.732
Sillero 3.91
Patrickios 1.888
IPC_peptide 3.783
IPC2_peptide 3.884
IPC2.peptide.svr19 3.831
Protein with the highest isoelectric point:
>tr|A0A3N2D223|A0A3N2D223_9MICO 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase OS=Salana multivorans OX=120377 GN=hisA PE=3 SV=1
MM1 pKa = 6.12 VTSVLVVPTTTGRR14 pKa = 11.84 RR15 pKa = 11.84 VARR18 pKa = 11.84 VTVPRR23 pKa = 11.84 VPTTTVRR30 pKa = 11.84 SVRR33 pKa = 11.84 TRR35 pKa = 11.84 SAVRR39 pKa = 11.84 VVTVLTVRR47 pKa = 11.84 SVPTRR52 pKa = 11.84 TVVLAARR59 pKa = 11.84 VTVPRR64 pKa = 11.84 VPTTTVRR71 pKa = 11.84 SVRR74 pKa = 11.84 TRR76 pKa = 11.84 TVVRR80 pKa = 11.84 VVTVLTVRR88 pKa = 11.84 SVPTRR93 pKa = 11.84 TVVLAARR100 pKa = 11.84 VTGPRR105 pKa = 11.84 VPTTTVRR112 pKa = 11.84 SVRR115 pKa = 11.84 TRR117 pKa = 11.84 TVVRR121 pKa = 11.84 VVTVLTVRR129 pKa = 11.84 SVPTTTAVPVARR141 pKa = 11.84 VTGPRR146 pKa = 11.84 VPTTTVRR153 pKa = 11.84 SVRR156 pKa = 11.84 TRR158 pKa = 11.84 TVVRR162 pKa = 11.84 AARR165 pKa = 11.84 VTGPRR170 pKa = 11.84 VPTTTGPSVRR180 pKa = 11.84 TRR182 pKa = 11.84 TAVRR186 pKa = 11.84 VATVLTGPRR195 pKa = 11.84 VPTTTAVRR203 pKa = 11.84 AVRR206 pKa = 11.84 AKK208 pKa = 9.27 TGRR211 pKa = 11.84 AARR214 pKa = 11.84 TVMTAPSVPMRR225 pKa = 11.84 TVARR229 pKa = 11.84 VATVTSVPVVLTTTGPSVPTRR250 pKa = 11.84 TVVRR254 pKa = 11.84 VVRR257 pKa = 11.84 ASVPRR262 pKa = 11.84 VPMVMTVRR270 pKa = 11.84 VARR273 pKa = 11.84 TATTVVRR280 pKa = 11.84 VVTVLTDD287 pKa = 3.56 PRR289 pKa = 11.84 APTVMTVRR297 pKa = 11.84 SVPTTTAALVVTVTSVPVVPTVMTVRR323 pKa = 11.84 SVRR326 pKa = 11.84 TRR328 pKa = 11.84 SAALVVRR335 pKa = 11.84 VSGPHH340 pKa = 5.77 VPTAMIVRR348 pKa = 11.84 SVLTTTAARR357 pKa = 11.84 VAAVTSAPVVRR368 pKa = 11.84 TATTVRR374 pKa = 11.84 SVPTTTAAHH383 pKa = 5.98 VVTAMIVRR391 pKa = 11.84 VAPTATTGGLAVTATTAAGARR412 pKa = 11.84 PTRR415 pKa = 11.84 ARR417 pKa = 11.84 LVRR420 pKa = 11.84 STSGVARR427 pKa = 11.84 TATSARR433 pKa = 11.84 ARR435 pKa = 11.84 TVAPSASTTRR445 pKa = 11.84 SSPRR449 pKa = 11.84 RR450 pKa = 11.84 SSSRR454 pKa = 11.84 CSRR457 pKa = 11.84 ARR459 pKa = 11.84 LARR462 pKa = 11.84 RR463 pKa = 11.84 CAAA466 pKa = 4.02
Molecular weight: 48.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.503
IPC2_protein 11.477
IPC_protein 13.1
Toseland 13.261
ProMoST 13.759
Dawson 13.261
Bjellqvist 13.261
Wikipedia 13.729
Rodwell 12.764
Grimsley 13.29
Solomon 13.759
Lehninger 13.656
Nozaki 13.261
DTASelect 13.261
Thurlkill 13.261
EMBOSS 13.759
Sillero 13.261
Patrickios 12.471
IPC_peptide 13.759
IPC2_peptide 12.749
IPC2.peptide.svr19 9.378
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3332
0
3332
1173099
30
3301
352.1
37.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.021 ± 0.059
0.55 ± 0.011
6.482 ± 0.036
5.74 ± 0.038
2.533 ± 0.023
9.605 ± 0.044
1.896 ± 0.02
3.362 ± 0.027
1.299 ± 0.024
10.239 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.584 ± 0.015
1.603 ± 0.022
6.039 ± 0.035
2.424 ± 0.022
7.722 ± 0.058
5.33 ± 0.031
6.385 ± 0.059
9.633 ± 0.041
1.644 ± 0.021
1.91 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here