Rodent arterivirus
Average proteome isoelectric point is 7.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4MYU6|A0A2H4MYU6_9NIDO GP5 protein OS=Rodent arterivirus OX=1806636 PE=4 SV=1
MM1 pKa = 7.34 GPSLLFMLVGVVGFHH16 pKa = 6.57 FSEE19 pKa = 5.77 GYY21 pKa = 9.62 ACKK24 pKa = 9.61 PCYY27 pKa = 9.27 GASYY31 pKa = 10.56 VDD33 pKa = 3.73 RR34 pKa = 11.84 ASKK37 pKa = 10.55 SSAVAPLAVTPLSCDD52 pKa = 3.26 RR53 pKa = 11.84 DD54 pKa = 3.49 EE55 pKa = 5.83 DD56 pKa = 4.13 KK57 pKa = 11.28 EE58 pKa = 4.28 KK59 pKa = 10.14 TLSLRR64 pKa = 11.84 GTPAMVCKK72 pKa = 10.56 QGDD75 pKa = 3.85 SKK77 pKa = 11.36 GFKK80 pKa = 9.97 INDD83 pKa = 3.46 VIEE86 pKa = 4.45 IKK88 pKa = 10.5 DD89 pKa = 3.89 DD90 pKa = 3.84 YY91 pKa = 11.04 SGSSADD97 pKa = 4.01 LLFFTACMIYY107 pKa = 10.04 GLEE110 pKa = 4.04 MAEE113 pKa = 4.32 KK114 pKa = 10.69 GLGPEE119 pKa = 4.97 LKK121 pKa = 9.83 NCSGTPCMCVPFSSWVAHH139 pKa = 4.82 VRR141 pKa = 11.84 EE142 pKa = 4.5 KK143 pKa = 11.33 GGDD146 pKa = 3.42 QQVQRR151 pKa = 11.84 GLFTNMSPHH160 pKa = 6.18 GLRR163 pKa = 11.84 WATVITCLLAILLAII178 pKa = 4.82
Molecular weight: 19.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.748
IPC2_protein 5.944
IPC_protein 5.97
Toseland 6.033
ProMoST 6.364
Dawson 6.313
Bjellqvist 6.275
Wikipedia 6.313
Rodwell 6.3
Grimsley 6.071
Solomon 6.313
Lehninger 6.313
Nozaki 6.576
DTASelect 6.722
Thurlkill 6.737
EMBOSS 6.722
Sillero 6.693
Patrickios 3.63
IPC_peptide 6.326
IPC2_peptide 6.576
IPC2.peptide.svr19 6.571
Protein with the highest isoelectric point:
>tr|A0A2H4MXK5|A0A2H4MXK5_9NIDO Membrane protein OS=Rodent arterivirus OX=1806636 PE=4 SV=1
MM1 pKa = 7.69 WPQLCIACSPMPLKK15 pKa = 10.95 SFLFLSLIFFFFLGSCSPSQLPGGSSSSWFSLFSLRR51 pKa = 11.84 PAARR55 pKa = 11.84 PVVLDD60 pKa = 3.17 MSSFQKK66 pKa = 10.48 MSDD69 pKa = 3.29 DD70 pKa = 4.16 AVRR73 pKa = 11.84 HH74 pKa = 5.45 CAAAIPPWLSHH85 pKa = 6.46 PLGIAFNGAIVDD97 pKa = 3.53 KK98 pKa = 9.77 MKK100 pKa = 10.87 RR101 pKa = 11.84 LIARR105 pKa = 11.84 LSMANYY111 pKa = 8.89 QGRR114 pKa = 11.84 VAWAEE119 pKa = 3.92 QQLLSPTLLRR129 pKa = 11.84 DD130 pKa = 4.02 LSNKK134 pKa = 9.36 TIVKK138 pKa = 9.59 HH139 pKa = 4.62 FVVMAGLEE147 pKa = 4.21 TGLCQYY153 pKa = 8.67 ITANMHH159 pKa = 5.87 RR160 pKa = 11.84 VMIAASASLRR170 pKa = 11.84 NMTITKK176 pKa = 10.47 NEE178 pKa = 4.08 TTGEE182 pKa = 4.05 WLIHH186 pKa = 5.66 RR187 pKa = 11.84 QQAPIEE193 pKa = 4.2 LNHH196 pKa = 6.62 FYY198 pKa = 10.8 KK199 pKa = 10.22 WVTWYY204 pKa = 10.42 RR205 pKa = 11.84 GSIFSAVSAALTLWVVILLRR225 pKa = 11.84 IRR227 pKa = 11.84 RR228 pKa = 11.84 RR229 pKa = 3.39
Molecular weight: 25.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 9.458
IPC_protein 9.78
Toseland 10.321
ProMoST 10.116
Dawson 10.467
Bjellqvist 10.16
Wikipedia 10.628
Rodwell 10.774
Grimsley 10.511
Solomon 10.526
Lehninger 10.496
Nozaki 10.365
DTASelect 10.131
Thurlkill 10.335
EMBOSS 10.716
Sillero 10.394
Patrickios 10.496
IPC_peptide 10.526
IPC2_peptide 9.311
IPC2.peptide.svr19 8.404
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
5110
70
3914
638.8
69.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.198 ± 0.471
3.151 ± 0.3
4.403 ± 0.832
4.07 ± 0.524
4.305 ± 0.528
6.986 ± 0.454
2.348 ± 0.248
4.266 ± 0.803
4.54 ± 0.424
9.902 ± 0.565
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.114 ± 0.477
3.131 ± 0.243
6.654 ± 1.099
3.405 ± 0.522
4.971 ± 0.337
7.378 ± 0.554
5.812 ± 0.262
8.454 ± 0.484
1.8 ± 0.504
3.112 ± 0.457
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here