Streptococcus phage Javan456

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D6B487|A0A4D6B487_9CAUD Uncharacterized protein OS=Streptococcus phage Javan456 OX=2548179 GN=Javan456_0021 PE=4 SV=1
MM1 pKa = 7.64NYY3 pKa = 10.44LEE5 pKa = 4.33YY6 pKa = 11.13ALAYY10 pKa = 10.27LEE12 pKa = 4.77RR13 pKa = 11.84EE14 pKa = 4.22LEE16 pKa = 4.5IIDD19 pKa = 3.9NEE21 pKa = 4.38VIEE24 pKa = 4.3VEE26 pKa = 4.36LPGGDD31 pKa = 3.75WEE33 pKa = 4.9CVPNPYY39 pKa = 9.59YY40 pKa = 10.6EE41 pKa = 5.04KK42 pKa = 10.67GLHH45 pKa = 6.74DD46 pKa = 4.15SPHH49 pKa = 5.83YY50 pKa = 9.94RR51 pKa = 11.84SQVAKK56 pKa = 10.58DD57 pKa = 3.09ILDD60 pKa = 3.46IKK62 pKa = 10.8GLLGRR67 pKa = 4.72

Molecular weight:
7.79 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D6B5T7|A0A4D6B5T7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan456 OX=2548179 GN=Javan456_0050 PE=4 SV=1
MM1 pKa = 7.55NKK3 pKa = 10.03RR4 pKa = 11.84IKK6 pKa = 10.22KK7 pKa = 9.09KK8 pKa = 10.34RR9 pKa = 11.84KK10 pKa = 9.22LEE12 pKa = 4.06TAVVMLVAEE21 pKa = 4.59NAMQAKK27 pKa = 10.09ALRR30 pKa = 11.84NQNKK34 pKa = 9.65QIAEE38 pKa = 4.03LRR40 pKa = 11.84AIIQQNVQAINRR52 pKa = 11.84EE53 pKa = 3.91FVTAKK58 pKa = 9.31NTILDD63 pKa = 3.6NQLAIKK69 pKa = 10.55LIGDD73 pKa = 3.77DD74 pKa = 3.78VGHH77 pKa = 7.1IKK79 pKa = 10.4QNYY82 pKa = 6.67KK83 pKa = 10.51RR84 pKa = 11.84KK85 pKa = 8.22WRR87 pKa = 11.84KK88 pKa = 8.88

Molecular weight:
10.25 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

13858

39

1211

216.5

24.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.519 ± 0.767

0.592 ± 0.119

6.271 ± 0.359

7.93 ± 0.595

4.019 ± 0.176

6.163 ± 0.516

1.422 ± 0.129

6.603 ± 0.204

9.042 ± 0.433

8.962 ± 0.406

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.41 ± 0.161

5.073 ± 0.26

2.634 ± 0.199

3.976 ± 0.238

4.156 ± 0.289

6.264 ± 0.507

5.968 ± 0.283

6.227 ± 0.3

1.162 ± 0.129

3.608 ± 0.297

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski