Streptococcus phage Javan456
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6B487|A0A4D6B487_9CAUD Uncharacterized protein OS=Streptococcus phage Javan456 OX=2548179 GN=Javan456_0021 PE=4 SV=1
MM1 pKa = 7.64 NYY3 pKa = 10.44 LEE5 pKa = 4.33 YY6 pKa = 11.13 ALAYY10 pKa = 10.27 LEE12 pKa = 4.77 RR13 pKa = 11.84 EE14 pKa = 4.22 LEE16 pKa = 4.5 IIDD19 pKa = 3.9 NEE21 pKa = 4.38 VIEE24 pKa = 4.3 VEE26 pKa = 4.36 LPGGDD31 pKa = 3.75 WEE33 pKa = 4.9 CVPNPYY39 pKa = 9.59 YY40 pKa = 10.6 EE41 pKa = 5.04 KK42 pKa = 10.67 GLHH45 pKa = 6.74 DD46 pKa = 4.15 SPHH49 pKa = 5.83 YY50 pKa = 9.94 RR51 pKa = 11.84 SQVAKK56 pKa = 10.58 DD57 pKa = 3.09 ILDD60 pKa = 3.46 IKK62 pKa = 10.8 GLLGRR67 pKa = 4.72
Molecular weight: 7.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.163
IPC2_protein 4.495
IPC_protein 4.355
Toseland 4.202
ProMoST 4.457
Dawson 4.291
Bjellqvist 4.444
Wikipedia 4.164
Rodwell 4.19
Grimsley 4.126
Solomon 4.291
Lehninger 4.24
Nozaki 4.418
DTASelect 4.533
Thurlkill 4.215
EMBOSS 4.177
Sillero 4.469
Patrickios 1.99
IPC_peptide 4.291
IPC2_peptide 4.457
IPC2.peptide.svr19 4.381
Protein with the highest isoelectric point:
>tr|A0A4D6B5T7|A0A4D6B5T7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan456 OX=2548179 GN=Javan456_0050 PE=4 SV=1
MM1 pKa = 7.55 NKK3 pKa = 10.03 RR4 pKa = 11.84 IKK6 pKa = 10.22 KK7 pKa = 9.09 KK8 pKa = 10.34 RR9 pKa = 11.84 KK10 pKa = 9.22 LEE12 pKa = 4.06 TAVVMLVAEE21 pKa = 4.59 NAMQAKK27 pKa = 10.09 ALRR30 pKa = 11.84 NQNKK34 pKa = 9.65 QIAEE38 pKa = 4.03 LRR40 pKa = 11.84 AIIQQNVQAINRR52 pKa = 11.84 EE53 pKa = 3.91 FVTAKK58 pKa = 9.31 NTILDD63 pKa = 3.6 NQLAIKK69 pKa = 10.55 LIGDD73 pKa = 3.77 DD74 pKa = 3.78 VGHH77 pKa = 7.1 IKK79 pKa = 10.4 QNYY82 pKa = 6.67 KK83 pKa = 10.51 RR84 pKa = 11.84 KK85 pKa = 8.22 WRR87 pKa = 11.84 KK88 pKa = 8.88
Molecular weight: 10.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.208
IPC2_protein 9.794
IPC_protein 10.058
Toseland 11.038
ProMoST 10.613
Dawson 11.082
Bjellqvist 10.701
Wikipedia 11.228
Rodwell 11.594
Grimsley 11.111
Solomon 11.184
Lehninger 11.169
Nozaki 11.008
DTASelect 10.701
Thurlkill 11.008
EMBOSS 11.418
Sillero 11.008
Patrickios 11.33
IPC_peptide 11.199
IPC2_peptide 9.077
IPC2.peptide.svr19 8.776
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
64
0
64
13858
39
1211
216.5
24.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.519 ± 0.767
0.592 ± 0.119
6.271 ± 0.359
7.93 ± 0.595
4.019 ± 0.176
6.163 ± 0.516
1.422 ± 0.129
6.603 ± 0.204
9.042 ± 0.433
8.962 ± 0.406
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.41 ± 0.161
5.073 ± 0.26
2.634 ± 0.199
3.976 ± 0.238
4.156 ± 0.289
6.264 ± 0.507
5.968 ± 0.283
6.227 ± 0.3
1.162 ± 0.129
3.608 ± 0.297
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here