Amino acid dipepetide frequency for Escherichia phage vB_EcoS_MM01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.71AlaAla: 8.71 ± 1.456
0.454AlaCys: 0.454 ± 0.188
3.636AlaAsp: 3.636 ± 0.5
6.211AlaGlu: 6.211 ± 0.976
2.954AlaPhe: 2.954 ± 0.508
6.589AlaGly: 6.589 ± 0.987
0.833AlaHis: 0.833 ± 0.293
6.665AlaIle: 6.665 ± 1.046
6.968AlaLys: 6.968 ± 1.041
7.12AlaLeu: 7.12 ± 0.937
1.969AlaMet: 1.969 ± 0.441
4.317AlaAsn: 4.317 ± 0.73
1.894AlaPro: 1.894 ± 0.282
3.484AlaGln: 3.484 ± 0.693
5.453AlaArg: 5.453 ± 1.096
5.756AlaSer: 5.756 ± 0.7
4.62AlaThr: 4.62 ± 0.575
5.983AlaVal: 5.983 ± 0.52
0.909AlaTrp: 0.909 ± 0.29
2.575AlaTyr: 2.575 ± 0.409
0.0AlaXaa: 0.0 ± 0.0
Cys
0.833CysAla: 0.833 ± 0.324
0.303CysCys: 0.303 ± 0.151
0.53CysAsp: 0.53 ± 0.157
0.682CysGlu: 0.682 ± 0.205
0.454CysPhe: 0.454 ± 0.217
1.288CysGly: 1.288 ± 0.356
0.227CysHis: 0.227 ± 0.131
0.303CysIle: 0.303 ± 0.145
1.06CysLys: 1.06 ± 0.331
0.909CysLeu: 0.909 ± 0.265
0.606CysMet: 0.606 ± 0.259
0.833CysAsn: 0.833 ± 0.273
0.379CysPro: 0.379 ± 0.166
0.076CysGln: 0.076 ± 0.07
0.682CysArg: 0.682 ± 0.232
1.212CysSer: 1.212 ± 0.292
0.757CysThr: 0.757 ± 0.235
0.454CysVal: 0.454 ± 0.151
0.227CysTrp: 0.227 ± 0.138
0.151CysTyr: 0.151 ± 0.113
0.0CysXaa: 0.0 ± 0.0
Asp
4.469AspAla: 4.469 ± 0.912
0.682AspCys: 0.682 ± 0.234
3.787AspAsp: 3.787 ± 0.562
3.863AspGlu: 3.863 ± 0.554
2.499AspPhe: 2.499 ± 0.444
6.135AspGly: 6.135 ± 0.705
0.606AspHis: 0.606 ± 0.242
3.711AspIle: 3.711 ± 0.443
4.317AspLys: 4.317 ± 0.529
4.014AspLeu: 4.014 ± 0.545
0.985AspMet: 0.985 ± 0.26
2.802AspAsn: 2.802 ± 0.509
1.742AspPro: 1.742 ± 0.431
1.439AspGln: 1.439 ± 0.276
2.499AspArg: 2.499 ± 0.494
4.469AspSer: 4.469 ± 0.725
2.954AspThr: 2.954 ± 0.509
4.62AspVal: 4.62 ± 0.634
1.288AspTrp: 1.288 ± 0.275
2.424AspTyr: 2.424 ± 0.379
0.0AspXaa: 0.0 ± 0.0
Glu
6.059GluAla: 6.059 ± 1.132
0.909GluCys: 0.909 ± 0.289
3.636GluAsp: 3.636 ± 0.523
5.075GluGlu: 5.075 ± 0.791
3.105GluPhe: 3.105 ± 0.46
4.241GluGly: 4.241 ± 0.457
0.606GluHis: 0.606 ± 0.204
4.999GluIle: 4.999 ± 0.522
4.62GluLys: 4.62 ± 0.685
5.453GluLeu: 5.453 ± 0.78
3.03GluMet: 3.03 ± 0.601
3.181GluAsn: 3.181 ± 0.533
1.969GluPro: 1.969 ± 0.343
2.727GluGln: 2.727 ± 0.531
3.105GluArg: 3.105 ± 0.55
4.09GluSer: 4.09 ± 0.55
3.484GluThr: 3.484 ± 0.42
5.605GluVal: 5.605 ± 0.87
0.303GluTrp: 0.303 ± 0.147
2.954GluTyr: 2.954 ± 0.473
0.0GluXaa: 0.0 ± 0.0
Phe
2.802PheAla: 2.802 ± 0.437
0.909PheCys: 0.909 ± 0.284
3.03PheAsp: 3.03 ± 0.477
3.333PheGlu: 3.333 ± 0.536
0.985PhePhe: 0.985 ± 0.269
3.56PheGly: 3.56 ± 0.491
0.682PheHis: 0.682 ± 0.2
2.196PheIle: 2.196 ± 0.364
2.727PheLys: 2.727 ± 0.498
1.818PheLeu: 1.818 ± 0.375
1.212PheMet: 1.212 ± 0.287
1.894PheAsn: 1.894 ± 0.349
1.06PhePro: 1.06 ± 0.283
1.894PheGln: 1.894 ± 0.378
2.121PheArg: 2.121 ± 0.302
2.348PheSer: 2.348 ± 0.449
2.878PheThr: 2.878 ± 0.391
2.348PheVal: 2.348 ± 0.39
0.53PheTrp: 0.53 ± 0.166
0.833PheTyr: 0.833 ± 0.229
0.0PheXaa: 0.0 ± 0.0
Gly
4.999GlyAla: 4.999 ± 0.585
1.288GlyCys: 1.288 ± 0.321
4.469GlyAsp: 4.469 ± 1.148
5.908GlyGlu: 5.908 ± 1.039
3.181GlyPhe: 3.181 ± 0.495
6.059GlyGly: 6.059 ± 1.207
0.757GlyHis: 0.757 ± 0.28
5.681GlyIle: 5.681 ± 0.61
5.453GlyLys: 5.453 ± 0.71
6.741GlyLeu: 6.741 ± 0.524
2.348GlyMet: 2.348 ± 0.468
3.636GlyAsn: 3.636 ± 0.465
2.272GlyPro: 2.272 ± 1.933
2.802GlyGln: 2.802 ± 0.417
2.954GlyArg: 2.954 ± 0.366
4.847GlySer: 4.847 ± 0.544
4.09GlyThr: 4.09 ± 0.587
5.605GlyVal: 5.605 ± 0.518
1.212GlyTrp: 1.212 ± 0.327
4.014GlyTyr: 4.014 ± 0.659
0.0GlyXaa: 0.0 ± 0.0
His
0.606HisAla: 0.606 ± 0.255
0.227HisCys: 0.227 ± 0.124
0.833HisAsp: 0.833 ± 0.24
1.06HisGlu: 1.06 ± 0.289
0.682HisPhe: 0.682 ± 0.21
0.757HisGly: 0.757 ± 0.313
0.303HisHis: 0.303 ± 0.154
1.212HisIle: 1.212 ± 0.345
1.288HisLys: 1.288 ± 0.318
0.909HisLeu: 0.909 ± 0.283
0.303HisMet: 0.303 ± 0.174
0.379HisAsn: 0.379 ± 0.158
0.379HisPro: 0.379 ± 0.187
0.379HisGln: 0.379 ± 0.165
0.757HisArg: 0.757 ± 0.231
1.06HisSer: 1.06 ± 0.341
0.833HisThr: 0.833 ± 0.288
0.757HisVal: 0.757 ± 0.232
0.076HisTrp: 0.076 ± 0.07
0.454HisTyr: 0.454 ± 0.166
0.0HisXaa: 0.0 ± 0.0
Ile
7.347IleAla: 7.347 ± 0.715
0.454IleCys: 0.454 ± 0.189
6.362IleAsp: 6.362 ± 0.499
3.408IleGlu: 3.408 ± 0.482
1.818IlePhe: 1.818 ± 0.334
4.847IleGly: 4.847 ± 0.62
1.06IleHis: 1.06 ± 0.24
3.105IleIle: 3.105 ± 0.602
4.544IleLys: 4.544 ± 0.539
2.424IleLeu: 2.424 ± 0.459
1.515IleMet: 1.515 ± 0.338
2.802IleAsn: 2.802 ± 0.553
2.499IlePro: 2.499 ± 0.327
2.196IleGln: 2.196 ± 0.579
2.727IleArg: 2.727 ± 0.391
4.62IleSer: 4.62 ± 0.664
5.15IleThr: 5.15 ± 0.549
4.317IleVal: 4.317 ± 0.65
0.909IleTrp: 0.909 ± 0.261
2.272IleTyr: 2.272 ± 0.382
0.0IleXaa: 0.0 ± 0.0
Lys
6.817LysAla: 6.817 ± 1.014
0.757LysCys: 0.757 ± 0.285
4.014LysAsp: 4.014 ± 0.555
4.923LysGlu: 4.923 ± 0.762
2.575LysPhe: 2.575 ± 0.431
4.393LysGly: 4.393 ± 1.348
1.136LysHis: 1.136 ± 0.236
4.09LysIle: 4.09 ± 0.474
4.014LysLys: 4.014 ± 0.637
5.681LysLeu: 5.681 ± 0.721
2.575LysMet: 2.575 ± 0.508
2.802LysAsn: 2.802 ± 0.496
2.348LysPro: 2.348 ± 0.354
2.121LysGln: 2.121 ± 0.447
3.636LysArg: 3.636 ± 0.763
4.317LysSer: 4.317 ± 0.693
4.014LysThr: 4.014 ± 0.533
4.166LysVal: 4.166 ± 0.715
0.757LysTrp: 0.757 ± 0.21
3.105LysTyr: 3.105 ± 0.574
0.0LysXaa: 0.0 ± 0.0
Leu
6.589LeuAla: 6.589 ± 0.809
0.909LeuCys: 0.909 ± 0.203
3.787LeuAsp: 3.787 ± 0.584
4.393LeuGlu: 4.393 ± 0.761
2.121LeuPhe: 2.121 ± 0.387
4.544LeuGly: 4.544 ± 0.736
0.909LeuHis: 0.909 ± 0.29
4.847LeuIle: 4.847 ± 0.647
4.166LeuLys: 4.166 ± 0.506
3.787LeuLeu: 3.787 ± 0.647
1.288LeuMet: 1.288 ± 0.313
3.03LeuAsn: 3.03 ± 0.463
2.727LeuPro: 2.727 ± 0.445
2.651LeuGln: 2.651 ± 0.688
3.56LeuArg: 3.56 ± 0.595
6.589LeuSer: 6.589 ± 0.643
4.241LeuThr: 4.241 ± 0.474
5.302LeuVal: 5.302 ± 0.563
0.53LeuTrp: 0.53 ± 0.228
1.818LeuTyr: 1.818 ± 0.365
0.0LeuXaa: 0.0 ± 0.0
Met
2.954MetAla: 2.954 ± 0.43
0.151MetCys: 0.151 ± 0.123
0.682MetAsp: 0.682 ± 0.253
1.288MetGlu: 1.288 ± 0.343
1.212MetPhe: 1.212 ± 0.334
0.757MetGly: 0.757 ± 0.204
0.379MetHis: 0.379 ± 0.148
1.969MetIle: 1.969 ± 0.37
2.196MetLys: 2.196 ± 0.408
1.742MetLeu: 1.742 ± 0.4
0.909MetMet: 0.909 ± 0.326
1.439MetAsn: 1.439 ± 0.391
0.379MetPro: 0.379 ± 0.182
0.909MetGln: 0.909 ± 0.254
1.666MetArg: 1.666 ± 0.317
1.136MetSer: 1.136 ± 0.273
2.348MetThr: 2.348 ± 0.421
1.894MetVal: 1.894 ± 0.389
0.606MetTrp: 0.606 ± 0.273
0.606MetTyr: 0.606 ± 0.207
0.0MetXaa: 0.0 ± 0.0
Asn
4.393AsnAla: 4.393 ± 0.655
0.606AsnCys: 0.606 ± 0.196
2.348AsnAsp: 2.348 ± 0.34
3.787AsnGlu: 3.787 ± 0.612
1.969AsnPhe: 1.969 ± 0.284
5.226AsnGly: 5.226 ± 0.958
1.136AsnHis: 1.136 ± 0.317
2.499AsnIle: 2.499 ± 0.401
2.878AsnLys: 2.878 ± 0.49
2.878AsnLeu: 2.878 ± 0.522
0.909AsnMet: 0.909 ± 0.237
3.105AsnAsn: 3.105 ± 0.48
2.272AsnPro: 2.272 ± 0.415
2.121AsnGln: 2.121 ± 0.459
1.969AsnArg: 1.969 ± 0.335
3.56AsnSer: 3.56 ± 0.426
1.894AsnThr: 1.894 ± 0.361
3.181AsnVal: 3.181 ± 0.372
1.136AsnTrp: 1.136 ± 0.27
1.818AsnTyr: 1.818 ± 0.385
0.0AsnXaa: 0.0 ± 0.0
Pro
3.484ProAla: 3.484 ± 0.502
0.606ProCys: 0.606 ± 0.277
2.196ProAsp: 2.196 ± 0.396
2.802ProGlu: 2.802 ± 0.373
1.515ProPhe: 1.515 ± 0.337
1.894ProGly: 1.894 ± 0.509
0.303ProHis: 0.303 ± 0.153
1.515ProIle: 1.515 ± 0.248
1.666ProLys: 1.666 ± 0.342
2.121ProLeu: 2.121 ± 0.444
0.833ProMet: 0.833 ± 0.218
1.288ProAsn: 1.288 ± 0.277
0.682ProPro: 0.682 ± 0.18
1.969ProGln: 1.969 ± 1.098
1.818ProArg: 1.818 ± 0.326
2.045ProSer: 2.045 ± 0.439
1.06ProThr: 1.06 ± 0.284
2.878ProVal: 2.878 ± 0.444
0.454ProTrp: 0.454 ± 0.196
1.439ProTyr: 1.439 ± 0.331
0.0ProXaa: 0.0 ± 0.0
Gln
3.181GlnAla: 3.181 ± 1.073
0.379GlnCys: 0.379 ± 0.154
1.363GlnAsp: 1.363 ± 0.352
2.651GlnGlu: 2.651 ± 0.499
1.591GlnPhe: 1.591 ± 0.293
4.014GlnGly: 4.014 ± 1.988
0.379GlnHis: 0.379 ± 0.16
2.878GlnIle: 2.878 ± 0.55
2.272GlnLys: 2.272 ± 0.368
2.575GlnLeu: 2.575 ± 0.45
0.606GlnMet: 0.606 ± 0.166
1.894GlnAsn: 1.894 ± 0.385
0.985GlnPro: 0.985 ± 0.306
3.105GlnGln: 3.105 ± 0.968
1.742GlnArg: 1.742 ± 0.398
3.03GlnSer: 3.03 ± 0.363
1.742GlnThr: 1.742 ± 0.357
2.499GlnVal: 2.499 ± 0.451
0.454GlnTrp: 0.454 ± 0.17
1.591GlnTyr: 1.591 ± 0.361
0.0GlnXaa: 0.0 ± 0.0
Arg
3.863ArgAla: 3.863 ± 0.638
0.682ArgCys: 0.682 ± 0.333
2.424ArgAsp: 2.424 ± 0.363
3.938ArgGlu: 3.938 ± 0.618
2.651ArgPhe: 2.651 ± 0.391
2.954ArgGly: 2.954 ± 0.603
0.53ArgHis: 0.53 ± 0.203
3.636ArgIle: 3.636 ± 0.46
3.863ArgLys: 3.863 ± 0.45
3.484ArgLeu: 3.484 ± 0.505
0.909ArgMet: 0.909 ± 0.306
1.818ArgAsn: 1.818 ± 0.357
1.288ArgPro: 1.288 ± 0.356
2.121ArgGln: 2.121 ± 0.442
2.424ArgArg: 2.424 ± 0.364
3.03ArgSer: 3.03 ± 0.48
1.894ArgThr: 1.894 ± 0.41
4.166ArgVal: 4.166 ± 0.416
0.379ArgTrp: 0.379 ± 0.168
2.272ArgTyr: 2.272 ± 0.369
0.0ArgXaa: 0.0 ± 0.0
Ser
6.059SerAla: 6.059 ± 0.721
0.454SerCys: 0.454 ± 0.187
5.378SerAsp: 5.378 ± 0.759
4.999SerGlu: 4.999 ± 0.569
3.408SerPhe: 3.408 ± 0.394
7.12SerGly: 7.12 ± 0.675
0.909SerHis: 0.909 ± 0.221
4.166SerIle: 4.166 ± 0.418
3.711SerLys: 3.711 ± 0.534
4.241SerLeu: 4.241 ± 0.615
1.894SerMet: 1.894 ± 0.341
3.181SerAsn: 3.181 ± 0.504
2.499SerPro: 2.499 ± 0.363
2.272SerGln: 2.272 ± 0.367
2.727SerArg: 2.727 ± 0.4
3.711SerSer: 3.711 ± 0.644
3.711SerThr: 3.711 ± 0.492
5.15SerVal: 5.15 ± 0.562
0.379SerTrp: 0.379 ± 0.153
2.954SerTyr: 2.954 ± 0.387
0.0SerXaa: 0.0 ± 0.0
Thr
5.075ThrAla: 5.075 ± 0.753
0.606ThrCys: 0.606 ± 0.272
2.499ThrAsp: 2.499 ± 0.357
2.954ThrGlu: 2.954 ± 0.459
2.045ThrPhe: 2.045 ± 0.397
5.605ThrGly: 5.605 ± 0.731
0.757ThrHis: 0.757 ± 0.306
3.863ThrIle: 3.863 ± 0.442
3.181ThrLys: 3.181 ± 0.499
3.711ThrLeu: 3.711 ± 0.603
0.757ThrMet: 0.757 ± 0.223
3.408ThrAsn: 3.408 ± 0.488
2.575ThrPro: 2.575 ± 0.348
2.499ThrGln: 2.499 ± 0.452
2.196ThrArg: 2.196 ± 0.35
3.408ThrSer: 3.408 ± 0.522
3.03ThrThr: 3.03 ± 0.496
4.014ThrVal: 4.014 ± 0.496
0.454ThrTrp: 0.454 ± 0.202
2.575ThrTyr: 2.575 ± 0.458
0.0ThrXaa: 0.0 ± 0.0
Val
5.681ValAla: 5.681 ± 0.795
0.909ValCys: 0.909 ± 0.275
5.075ValAsp: 5.075 ± 0.596
4.317ValGlu: 4.317 ± 0.718
2.575ValPhe: 2.575 ± 0.396
4.317ValGly: 4.317 ± 0.709
0.909ValHis: 0.909 ± 0.234
4.09ValIle: 4.09 ± 0.606
5.756ValLys: 5.756 ± 0.711
4.544ValLeu: 4.544 ± 0.649
1.666ValMet: 1.666 ± 0.371
5.681ValAsn: 5.681 ± 0.685
2.424ValPro: 2.424 ± 0.332
2.121ValGln: 2.121 ± 0.482
3.408ValArg: 3.408 ± 0.512
6.059ValSer: 6.059 ± 0.565
3.03ValThr: 3.03 ± 0.597
5.529ValVal: 5.529 ± 0.873
0.606ValTrp: 0.606 ± 0.178
3.408ValTyr: 3.408 ± 0.545
0.0ValXaa: 0.0 ± 0.0
Trp
0.757TrpAla: 0.757 ± 0.216
0.151TrpCys: 0.151 ± 0.114
0.757TrpAsp: 0.757 ± 0.214
0.833TrpGlu: 0.833 ± 0.264
0.606TrpPhe: 0.606 ± 0.26
0.833TrpGly: 0.833 ± 0.223
0.454TrpHis: 0.454 ± 0.151
0.757TrpIle: 0.757 ± 0.205
1.06TrpLys: 1.06 ± 0.271
0.833TrpLeu: 0.833 ± 0.217
0.076TrpMet: 0.076 ± 0.077
0.151TrpAsn: 0.151 ± 0.107
0.379TrpPro: 0.379 ± 0.147
0.53TrpGln: 0.53 ± 0.217
1.06TrpArg: 1.06 ± 0.244
0.606TrpSer: 0.606 ± 0.289
0.53TrpThr: 0.53 ± 0.246
0.985TrpVal: 0.985 ± 0.283
0.076TrpTrp: 0.076 ± 0.078
0.303TrpTyr: 0.303 ± 0.13
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.499TyrAla: 2.499 ± 0.485
0.53TyrCys: 0.53 ± 0.209
2.499TyrAsp: 2.499 ± 0.418
2.727TyrGlu: 2.727 ± 0.406
1.212TyrPhe: 1.212 ± 0.332
3.181TyrGly: 3.181 ± 0.459
0.379TyrHis: 0.379 ± 0.157
2.196TyrIle: 2.196 ± 0.415
2.499TyrLys: 2.499 ± 0.454
2.802TyrLeu: 2.802 ± 0.468
0.757TyrMet: 0.757 ± 0.195
2.045TyrAsn: 2.045 ± 0.406
1.818TyrPro: 1.818 ± 0.474
1.515TyrGln: 1.515 ± 0.345
1.818TyrArg: 1.818 ± 0.357
3.03TyrSer: 3.03 ± 0.514
2.954TyrThr: 2.954 ± 0.354
2.651TyrVal: 2.651 ± 0.471
0.454TyrTrp: 0.454 ± 0.154
1.06TyrTyr: 1.06 ± 0.235
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (13204 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski