Klebsiella phage 1 TK-2018

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Slopekvirinae; Drulisvirus; unclassified Drulisvirus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5K7NIE8|A0A5K7NIE8_9CAUD Uncharacterized protein OS=Klebsiella phage 1 TK-2018 OX=2489637 PE=4 SV=1
MM1 pKa = 7.9DD2 pKa = 4.91KK3 pKa = 10.93VLEE6 pKa = 4.58AYY8 pKa = 8.27EE9 pKa = 4.04NLAITVGSAVYY20 pKa = 10.1DD21 pKa = 3.64AAAYY25 pKa = 9.2GVLNISLDD33 pKa = 4.25DD34 pKa = 4.02IYY36 pKa = 11.75NALDD40 pKa = 3.66EE41 pKa = 4.62LAALYY46 pKa = 10.96GMDD49 pKa = 4.95LEE51 pKa = 4.69LAATAFRR58 pKa = 11.84EE59 pKa = 4.34HH60 pKa = 6.89NDD62 pKa = 3.38LAAHH66 pKa = 6.6ADD68 pKa = 3.51KK69 pKa = 11.22LRR71 pKa = 11.84GDD73 pKa = 3.97EE74 pKa = 4.12LVLIRR79 pKa = 11.84VVGTLSIGLAEE90 pKa = 4.81IGSCIYY96 pKa = 10.87DD97 pKa = 3.05VDD99 pKa = 3.62QSLRR103 pKa = 11.84TPEE106 pKa = 4.5VIGDD110 pKa = 3.64MLGTVLVLSEE120 pKa = 5.27LEE122 pKa = 4.21DD123 pKa = 3.46

Molecular weight: 13.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5K7NJ87|A0A5K7NJ87_9CAUD Uncharacterized protein OS=Klebsiella phage 1 TK-2018 OX=2489637 PE=4 SV=1
MM1 pKa = 7.28GVRR4 pKa = 11.84VKK6 pKa = 10.62VSAPKK11 pKa = 9.6HH12 pKa = 5.95CFMEE16 pKa = 5.34PYY18 pKa = 9.76TEE20 pKa = 4.17RR21 pKa = 11.84ACNRR25 pKa = 11.84WYY27 pKa = 10.96VIFNRR32 pKa = 11.84EE33 pKa = 3.78DD34 pKa = 3.27NPRR37 pKa = 11.84NMYY40 pKa = 8.31VVRR43 pKa = 11.84WAAKK47 pKa = 6.48PTRR50 pKa = 11.84KK51 pKa = 9.04QVKK54 pKa = 9.18LAAKK58 pKa = 9.69SVARR62 pKa = 11.84LEE64 pKa = 4.15II65 pKa = 4.42

Molecular weight: 7.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

12521

51

1232

260.9

28.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.638 ± 0.628

1.094 ± 0.197

6.23 ± 0.231

5.87 ± 0.388

3.187 ± 0.147

7.547 ± 0.27

1.821 ± 0.191

4.297 ± 0.163

4.864 ± 0.29

8.633 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.915 ± 0.224

4.065 ± 0.248

3.786 ± 0.322

4.784 ± 0.366

5.838 ± 0.294

6.381 ± 0.381

5.67 ± 0.289

7.164 ± 0.276

1.39 ± 0.113

3.826 ± 0.257
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski