Amino acid dipepetide frequency for Bacillus phage Hyb1phi3Ts-SPbeta

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.734AlaAla: 3.734 ± 0.384
0.5AlaCys: 0.5 ± 0.142
3.392AlaAsp: 3.392 ± 0.327
3.05AlaGlu: 3.05 ± 0.327
1.998AlaPhe: 1.998 ± 0.255
2.787AlaGly: 2.787 ± 0.35
0.763AlaHis: 0.763 ± 0.146
4.418AlaIle: 4.418 ± 0.334
4.996AlaLys: 4.996 ± 0.523
4.207AlaLeu: 4.207 ± 0.386
1.288AlaMet: 1.288 ± 0.184
2.551AlaAsn: 2.551 ± 0.295
1.104AlaPro: 1.104 ± 0.16
1.657AlaGln: 1.657 ± 0.327
1.814AlaArg: 1.814 ± 0.201
3.024AlaSer: 3.024 ± 0.376
2.761AlaThr: 2.761 ± 0.229
3.077AlaVal: 3.077 ± 0.329
0.473AlaTrp: 0.473 ± 0.103
2.577AlaTyr: 2.577 ± 0.271
0.0AlaXaa: 0.0 ± 0.0
Cys
0.394CysAla: 0.394 ± 0.113
0.158CysCys: 0.158 ± 0.06
0.342CysAsp: 0.342 ± 0.1
0.631CysGlu: 0.631 ± 0.126
0.789CysPhe: 0.789 ± 0.139
0.92CysGly: 0.92 ± 0.2
0.342CysHis: 0.342 ± 0.092
0.631CysIle: 0.631 ± 0.152
0.684CysLys: 0.684 ± 0.19
0.868CysLeu: 0.868 ± 0.151
0.184CysMet: 0.184 ± 0.073
0.763CysAsn: 0.763 ± 0.151
0.342CysPro: 0.342 ± 0.111
0.316CysGln: 0.316 ± 0.088
0.5CysArg: 0.5 ± 0.122
0.657CysSer: 0.657 ± 0.136
0.473CysThr: 0.473 ± 0.12
0.578CysVal: 0.578 ± 0.127
0.237CysTrp: 0.237 ± 0.08
0.447CysTyr: 0.447 ± 0.129
0.0CysXaa: 0.0 ± 0.0
Asp
2.656AspAla: 2.656 ± 0.27
0.763AspCys: 0.763 ± 0.16
3.708AspAsp: 3.708 ± 0.367
5.049AspGlu: 5.049 ± 0.337
3.208AspPhe: 3.208 ± 0.241
4.049AspGly: 4.049 ± 0.363
1.026AspHis: 1.026 ± 0.191
5.443AspIle: 5.443 ± 0.325
5.89AspLys: 5.89 ± 0.502
5.312AspLeu: 5.312 ± 0.338
1.262AspMet: 1.262 ± 0.144
3.392AspAsn: 3.392 ± 0.295
2.025AspPro: 2.025 ± 0.212
2.235AspGln: 2.235 ± 0.24
1.841AspArg: 1.841 ± 0.243
3.865AspSer: 3.865 ± 0.291
2.498AspThr: 2.498 ± 0.264
3.839AspVal: 3.839 ± 0.313
0.552AspTrp: 0.552 ± 0.116
3.629AspTyr: 3.629 ± 0.283
0.0AspXaa: 0.0 ± 0.0
Glu
4.155GluAla: 4.155 ± 0.382
0.815GluCys: 0.815 ± 0.169
4.654GluAsp: 4.654 ± 0.358
6.574GluGlu: 6.574 ± 0.491
3.418GluPhe: 3.418 ± 0.285
3.76GluGly: 3.76 ± 0.352
1.578GluHis: 1.578 ± 0.264
7.021GluIle: 7.021 ± 0.398
7.363GluLys: 7.363 ± 0.445
8.204GluLeu: 8.204 ± 0.481
2.551GluMet: 2.551 ± 0.267
4.575GluAsn: 4.575 ± 0.366
1.578GluPro: 1.578 ± 0.24
3.208GluGln: 3.208 ± 0.351
3.313GluArg: 3.313 ± 0.345
4.076GluSer: 4.076 ± 0.364
3.524GluThr: 3.524 ± 0.3
5.417GluVal: 5.417 ± 0.46
1.288GluTrp: 1.288 ± 0.233
3.813GluTyr: 3.813 ± 0.313
0.0GluXaa: 0.0 ± 0.0
Phe
1.92PheAla: 1.92 ± 0.218
0.447PheCys: 0.447 ± 0.127
3.313PheAsp: 3.313 ± 0.294
3.892PheGlu: 3.892 ± 0.328
2.393PhePhe: 2.393 ± 0.327
2.393PheGly: 2.393 ± 0.227
1.052PheHis: 1.052 ± 0.165
3.313PheIle: 3.313 ± 0.301
4.365PheLys: 4.365 ± 0.362
3.55PheLeu: 3.55 ± 0.386
0.815PheMet: 0.815 ± 0.149
3.077PheAsn: 3.077 ± 0.259
1.341PhePro: 1.341 ± 0.153
0.815PheGln: 0.815 ± 0.138
1.735PheArg: 1.735 ± 0.19
2.998PheSer: 2.998 ± 0.277
2.209PheThr: 2.209 ± 0.234
2.393PheVal: 2.393 ± 0.316
0.263PheTrp: 0.263 ± 0.083
2.261PheTyr: 2.261 ± 0.286
0.0PheXaa: 0.0 ± 0.0
Gly
2.472GlyAla: 2.472 ± 0.235
0.552GlyCys: 0.552 ± 0.123
3.129GlyAsp: 3.129 ± 0.299
4.233GlyGlu: 4.233 ± 0.37
2.814GlyPhe: 2.814 ± 0.217
2.84GlyGly: 2.84 ± 0.361
1.078GlyHis: 1.078 ± 0.182
4.181GlyIle: 4.181 ± 0.343
5.075GlyLys: 5.075 ± 0.369
4.47GlyLeu: 4.47 ± 0.324
1.341GlyMet: 1.341 ± 0.218
3.392GlyAsn: 3.392 ± 0.338
0.131GlyPro: 0.131 ± 0.053
2.025GlyGln: 2.025 ± 0.206
1.709GlyArg: 1.709 ± 0.226
3.392GlySer: 3.392 ± 0.325
3.524GlyThr: 3.524 ± 0.289
3.261GlyVal: 3.261 ± 0.283
0.473GlyTrp: 0.473 ± 0.106
2.814GlyTyr: 2.814 ± 0.277
0.0GlyXaa: 0.0 ± 0.0
His
0.552HisAla: 0.552 ± 0.124
0.316HisCys: 0.316 ± 0.098
0.868HisAsp: 0.868 ± 0.133
1.604HisGlu: 1.604 ± 0.228
0.736HisPhe: 0.736 ± 0.138
1.026HisGly: 1.026 ± 0.16
0.368HisHis: 0.368 ± 0.09
1.367HisIle: 1.367 ± 0.23
1.841HisLys: 1.841 ± 0.252
1.63HisLeu: 1.63 ± 0.189
0.368HisMet: 0.368 ± 0.108
1.551HisAsn: 1.551 ± 0.214
0.631HisPro: 0.631 ± 0.115
0.605HisGln: 0.605 ± 0.131
0.841HisArg: 0.841 ± 0.155
1.236HisSer: 1.236 ± 0.193
1.157HisThr: 1.157 ± 0.183
1.078HisVal: 1.078 ± 0.162
0.158HisTrp: 0.158 ± 0.06
0.894HisTyr: 0.894 ± 0.166
0.0HisXaa: 0.0 ± 0.0
Ile
4.049IleAla: 4.049 ± 0.291
0.684IleCys: 0.684 ± 0.145
5.627IleAsp: 5.627 ± 0.374
6.679IleGlu: 6.679 ± 0.385
2.577IlePhe: 2.577 ± 0.28
4.233IleGly: 4.233 ± 0.305
1.946IleHis: 1.946 ± 0.29
4.628IleIle: 4.628 ± 0.486
8.414IleLys: 8.414 ± 0.482
5.443IleLeu: 5.443 ± 0.538
1.42IleMet: 1.42 ± 0.205
5.89IleAsn: 5.89 ± 0.381
2.419IlePro: 2.419 ± 0.225
2.603IleGln: 2.603 ± 0.281
2.419IleArg: 2.419 ± 0.22
4.838IleSer: 4.838 ± 0.369
4.339IleThr: 4.339 ± 0.387
4.418IleVal: 4.418 ± 0.403
0.868IleTrp: 0.868 ± 0.153
2.235IleTyr: 2.235 ± 0.266
0.0IleXaa: 0.0 ± 0.0
Lys
5.364LysAla: 5.364 ± 0.456
1.078LysCys: 1.078 ± 0.228
5.68LysAsp: 5.68 ± 0.366
9.387LysGlu: 9.387 ± 0.615
3.629LysPhe: 3.629 ± 0.328
5.469LysGly: 5.469 ± 0.414
1.841LysHis: 1.841 ± 0.243
6.574LysIle: 6.574 ± 0.41
10.308LysLys: 10.308 ± 0.571
8.546LysLeu: 8.546 ± 0.532
2.288LysMet: 2.288 ± 0.264
5.89LysAsn: 5.89 ± 0.5
2.34LysPro: 2.34 ± 0.304
3.944LysGln: 3.944 ± 0.347
4.155LysArg: 4.155 ± 0.436
5.759LysSer: 5.759 ± 0.467
5.128LysThr: 5.128 ± 0.433
6.1LysVal: 6.1 ± 0.441
0.815LysTrp: 0.815 ± 0.151
4.365LysTyr: 4.365 ± 0.374
0.0LysXaa: 0.0 ± 0.0
Leu
4.207LeuAla: 4.207 ± 0.35
0.841LeuCys: 0.841 ± 0.162
5.206LeuAsp: 5.206 ± 0.318
6.442LeuGlu: 6.442 ± 0.501
4.128LeuPhe: 4.128 ± 0.366
3.708LeuGly: 3.708 ± 0.265
1.578LeuHis: 1.578 ± 0.18
6.679LeuIle: 6.679 ± 0.54
9.045LeuLys: 9.045 ± 0.516
7.652LeuLeu: 7.652 ± 0.463
2.13LeuMet: 2.13 ± 0.236
6.758LeuAsn: 6.758 ± 0.441
2.445LeuPro: 2.445 ± 0.298
3.497LeuGln: 3.497 ± 0.45
3.524LeuArg: 3.524 ± 0.357
6.074LeuSer: 6.074 ± 0.399
5.285LeuThr: 5.285 ± 0.316
4.391LeuVal: 4.391 ± 0.326
0.868LeuTrp: 0.868 ± 0.169
3.313LeuTyr: 3.313 ± 0.337
0.0LeuXaa: 0.0 ± 0.0
Met
1.42MetAla: 1.42 ± 0.172
0.079MetCys: 0.079 ± 0.048
1.499MetAsp: 1.499 ± 0.207
1.788MetGlu: 1.788 ± 0.213
1.052MetPhe: 1.052 ± 0.18
1.341MetGly: 1.341 ± 0.185
0.263MetHis: 0.263 ± 0.082
1.604MetIle: 1.604 ± 0.232
2.656MetLys: 2.656 ± 0.277
2.025MetLeu: 2.025 ± 0.23
0.631MetMet: 0.631 ± 0.119
1.709MetAsn: 1.709 ± 0.209
0.631MetPro: 0.631 ± 0.124
0.789MetGln: 0.789 ± 0.19
1.026MetArg: 1.026 ± 0.179
1.735MetSer: 1.735 ± 0.194
1.367MetThr: 1.367 ± 0.201
1.052MetVal: 1.052 ± 0.148
0.263MetTrp: 0.263 ± 0.092
0.552MetTyr: 0.552 ± 0.107
0.0MetXaa: 0.0 ± 0.0
Asn
3.313AsnAla: 3.313 ± 0.395
0.841AsnCys: 0.841 ± 0.162
3.602AsnAsp: 3.602 ± 0.25
5.443AsnGlu: 5.443 ± 0.353
2.708AsnPhe: 2.708 ± 0.258
3.471AsnGly: 3.471 ± 0.287
1.026AsnHis: 1.026 ± 0.173
5.049AsnIle: 5.049 ± 0.35
7.52AsnLys: 7.52 ± 0.479
5.285AsnLeu: 5.285 ± 0.367
1.446AsnMet: 1.446 ± 0.179
4.207AsnAsn: 4.207 ± 0.321
1.867AsnPro: 1.867 ± 0.243
1.998AsnGln: 1.998 ± 0.221
2.551AsnArg: 2.551 ± 0.241
3.971AsnSer: 3.971 ± 0.393
3.155AsnThr: 3.155 ± 0.379
3.445AsnVal: 3.445 ± 0.34
0.526AsnTrp: 0.526 ± 0.134
2.63AsnTyr: 2.63 ± 0.256
0.0AsnXaa: 0.0 ± 0.0
Pro
1.236ProAla: 1.236 ± 0.193
0.237ProCys: 0.237 ± 0.077
2.288ProAsp: 2.288 ± 0.213
2.156ProGlu: 2.156 ± 0.264
1.104ProPhe: 1.104 ± 0.153
0.947ProGly: 0.947 ± 0.16
0.868ProHis: 0.868 ± 0.158
1.604ProIle: 1.604 ± 0.23
2.708ProLys: 2.708 ± 0.304
2.656ProLeu: 2.656 ± 0.303
0.473ProMet: 0.473 ± 0.127
1.473ProAsn: 1.473 ± 0.153
0.578ProPro: 0.578 ± 0.118
0.578ProGln: 0.578 ± 0.11
0.815ProArg: 0.815 ± 0.16
1.946ProSer: 1.946 ± 0.214
1.315ProThr: 1.315 ± 0.205
1.262ProVal: 1.262 ± 0.227
0.263ProTrp: 0.263 ± 0.1
1.394ProTyr: 1.394 ± 0.174
0.0ProXaa: 0.0 ± 0.0
Gln
1.998GlnAla: 1.998 ± 0.322
0.316GlnCys: 0.316 ± 0.099
1.63GlnAsp: 1.63 ± 0.222
3.024GlnGlu: 3.024 ± 0.343
1.367GlnPhe: 1.367 ± 0.191
1.473GlnGly: 1.473 ± 0.218
0.578GlnHis: 0.578 ± 0.129
2.656GlnIle: 2.656 ± 0.281
3.339GlnLys: 3.339 ± 0.462
3.944GlnLeu: 3.944 ± 0.367
0.999GlnMet: 0.999 ± 0.15
2.367GlnAsn: 2.367 ± 0.321
0.736GlnPro: 0.736 ± 0.117
1.946GlnGln: 1.946 ± 0.471
1.262GlnArg: 1.262 ± 0.169
2.13GlnSer: 2.13 ± 0.283
1.42GlnThr: 1.42 ± 0.206
2.314GlnVal: 2.314 ± 0.208
0.394GlnTrp: 0.394 ± 0.109
1.657GlnTyr: 1.657 ± 0.186
0.0GlnXaa: 0.0 ± 0.0
Arg
1.657ArgAla: 1.657 ± 0.204
0.316ArgCys: 0.316 ± 0.096
2.235ArgAsp: 2.235 ± 0.22
3.129ArgGlu: 3.129 ± 0.328
1.92ArgPhe: 1.92 ± 0.225
1.998ArgGly: 1.998 ± 0.252
0.815ArgHis: 0.815 ± 0.162
3.024ArgIle: 3.024 ± 0.316
3.208ArgLys: 3.208 ± 0.267
3.103ArgLeu: 3.103 ± 0.355
1.236ArgMet: 1.236 ± 0.195
2.945ArgAsn: 2.945 ± 0.325
0.947ArgPro: 0.947 ± 0.169
1.394ArgGln: 1.394 ± 0.172
1.683ArgArg: 1.683 ± 0.238
1.998ArgSer: 1.998 ± 0.252
1.841ArgThr: 1.841 ± 0.232
2.314ArgVal: 2.314 ± 0.237
0.394ArgTrp: 0.394 ± 0.098
1.92ArgTyr: 1.92 ± 0.207
0.0ArgXaa: 0.0 ± 0.0
Ser
2.708SerAla: 2.708 ± 0.338
0.657SerCys: 0.657 ± 0.153
4.233SerAsp: 4.233 ± 0.375
4.681SerGlu: 4.681 ± 0.356
3.287SerPhe: 3.287 ± 0.297
3.445SerGly: 3.445 ± 0.328
0.894SerHis: 0.894 ± 0.165
5.022SerIle: 5.022 ± 0.511
5.68SerLys: 5.68 ± 0.347
6.6SerLeu: 6.6 ± 0.468
1.525SerMet: 1.525 ± 0.217
4.128SerAsn: 4.128 ± 0.264
2.13SerPro: 2.13 ± 0.26
1.946SerGln: 1.946 ± 0.214
1.946SerArg: 1.946 ± 0.253
5.18SerSer: 5.18 ± 0.573
3.392SerThr: 3.392 ± 0.415
3.602SerVal: 3.602 ± 0.259
0.578SerTrp: 0.578 ± 0.112
2.498SerTyr: 2.498 ± 0.261
0.0SerXaa: 0.0 ± 0.0
Thr
2.919ThrAla: 2.919 ± 0.42
0.316ThrCys: 0.316 ± 0.093
2.866ThrAsp: 2.866 ± 0.292
4.049ThrGlu: 4.049 ± 0.294
2.077ThrPhe: 2.077 ± 0.238
3.418ThrGly: 3.418 ± 0.283
0.71ThrHis: 0.71 ± 0.145
3.76ThrIle: 3.76 ± 0.292
4.391ThrLys: 4.391 ± 0.329
4.339ThrLeu: 4.339 ± 0.357
0.999ThrMet: 0.999 ± 0.165
2.682ThrAsn: 2.682 ± 0.32
1.683ThrPro: 1.683 ± 0.174
2.156ThrGln: 2.156 ± 0.281
2.077ThrArg: 2.077 ± 0.204
3.655ThrSer: 3.655 ± 0.368
2.814ThrThr: 2.814 ± 0.308
4.26ThrVal: 4.26 ± 0.306
0.526ThrTrp: 0.526 ± 0.125
2.892ThrTyr: 2.892 ± 0.265
0.0ThrXaa: 0.0 ± 0.0
Val
3.524ValAla: 3.524 ± 0.274
0.763ValCys: 0.763 ± 0.153
3.865ValAsp: 3.865 ± 0.33
4.339ValGlu: 4.339 ± 0.358
2.524ValPhe: 2.524 ± 0.269
3.077ValGly: 3.077 ± 0.321
1.21ValHis: 1.21 ± 0.174
4.47ValIle: 4.47 ± 0.391
5.785ValLys: 5.785 ± 0.404
4.97ValLeu: 4.97 ± 0.431
1.42ValMet: 1.42 ± 0.21
3.892ValAsn: 3.892 ± 0.36
1.657ValPro: 1.657 ± 0.215
1.998ValGln: 1.998 ± 0.256
2.235ValArg: 2.235 ± 0.269
3.813ValSer: 3.813 ± 0.321
3.339ValThr: 3.339 ± 0.311
3.339ValVal: 3.339 ± 0.355
0.5ValTrp: 0.5 ± 0.116
2.551ValTyr: 2.551 ± 0.304
0.0ValXaa: 0.0 ± 0.0
Trp
0.447TrpAla: 0.447 ± 0.089
0.184TrpCys: 0.184 ± 0.071
0.841TrpAsp: 0.841 ± 0.16
0.92TrpGlu: 0.92 ± 0.193
0.421TrpPhe: 0.421 ± 0.103
0.447TrpGly: 0.447 ± 0.111
0.131TrpHis: 0.131 ± 0.057
0.736TrpIle: 0.736 ± 0.167
0.841TrpLys: 0.841 ± 0.15
0.947TrpLeu: 0.947 ± 0.169
0.184TrpMet: 0.184 ± 0.07
0.394TrpAsn: 0.394 ± 0.143
0.105TrpPro: 0.105 ± 0.047
0.316TrpGln: 0.316 ± 0.105
0.526TrpArg: 0.526 ± 0.132
0.631TrpSer: 0.631 ± 0.125
0.578TrpThr: 0.578 ± 0.113
0.657TrpVal: 0.657 ± 0.101
0.079TrpTrp: 0.079 ± 0.046
0.578TrpTyr: 0.578 ± 0.136
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.446TyrAla: 1.446 ± 0.192
0.394TyrCys: 0.394 ± 0.105
3.261TyrAsp: 3.261 ± 0.3
3.813TyrGlu: 3.813 ± 0.372
2.393TyrPhe: 2.393 ± 0.249
2.104TyrGly: 2.104 ± 0.195
0.763TyrHis: 0.763 ± 0.136
3.576TyrIle: 3.576 ± 0.275
4.549TyrLys: 4.549 ± 0.396
4.023TyrLeu: 4.023 ± 0.323
0.894TyrMet: 0.894 ± 0.167
2.419TyrAsn: 2.419 ± 0.308
1.21TyrPro: 1.21 ± 0.172
1.525TyrGln: 1.525 ± 0.179
2.104TyrArg: 2.104 ± 0.25
3.155TyrSer: 3.155 ± 0.343
2.472TyrThr: 2.472 ± 0.218
2.472TyrVal: 2.472 ± 0.278
0.447TyrTrp: 0.447 ± 0.121
1.972TyrTyr: 1.972 ± 0.224
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 196 proteins (38031 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski