Monosiga brevicollis (Choanoflagellate)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Choanoflagellata; Craspedida; Salpingoecidae; Monosiga

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9188 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A9UQI2|A9UQI2_MONBE Uncharacterized protein OS=Monosiga brevicollis OX=81824 GN=21077 PE=3 SV=1
MM1 pKa = 7.6GLRR4 pKa = 11.84RR5 pKa = 11.84SWAGFCLGVGLVLVLVACDD24 pKa = 3.15VGAGVEE30 pKa = 4.3EE31 pKa = 4.54PSLAFDD37 pKa = 3.75EE38 pKa = 4.98NGTLFINATNRR49 pKa = 11.84VVVNGVDD56 pKa = 3.76VQALAMAFSAASSSLDD72 pKa = 3.59ALIEE76 pKa = 4.02LQRR79 pKa = 11.84EE80 pKa = 4.15LNPPQFEE87 pKa = 3.94QHH89 pKa = 6.55AISATMDD96 pKa = 3.67GSRR99 pKa = 11.84SACAADD105 pKa = 3.89LDD107 pKa = 4.46GDD109 pKa = 4.05GFLDD113 pKa = 3.85VLSTNYY119 pKa = 9.79LQHH122 pKa = 6.18QISWYY127 pKa = 9.77RR128 pKa = 11.84NNGDD132 pKa = 3.95GSFSDD137 pKa = 4.22QIVITNTSQSVWYY150 pKa = 7.26ATPADD155 pKa = 4.02LSGDD159 pKa = 3.73GQLDD163 pKa = 3.63IVSVWHH169 pKa = 6.74NDD171 pKa = 3.05MIAWYY176 pKa = 10.15LNTGNGAFSPEE187 pKa = 3.87PMIITTDD194 pKa = 3.3VKK196 pKa = 10.7AGRR199 pKa = 11.84SVFATDD205 pKa = 4.33FNNDD209 pKa = 2.64GYY211 pKa = 9.46TDD213 pKa = 3.8VVSASMDD220 pKa = 3.63DD221 pKa = 6.07DD222 pKa = 4.65KK223 pKa = 11.49IAWYY227 pKa = 10.12RR228 pKa = 11.84NNGNSTFSAQIIISTDD244 pKa = 2.97AVEE247 pKa = 4.35ALSVYY252 pKa = 10.64AADD255 pKa = 4.52LNNDD259 pKa = 3.11GFADD263 pKa = 3.85VLSASPGDD271 pKa = 5.26DD272 pKa = 2.67KK273 pKa = 11.72VAWYY277 pKa = 10.19RR278 pKa = 11.84NNGNGTFTNQIVITTSADD296 pKa = 3.5GPVSVYY302 pKa = 10.82AADD305 pKa = 4.21LDD307 pKa = 4.52GDD309 pKa = 3.83GHH311 pKa = 8.82LDD313 pKa = 3.6VLSASYY319 pKa = 11.01LDD321 pKa = 5.87NKK323 pKa = 10.04IAWCRR328 pKa = 11.84NNGNGTFSEE337 pKa = 4.17QLVISDD343 pKa = 4.55AADD346 pKa = 3.25MAYY349 pKa = 10.22SVYY352 pKa = 10.83AADD355 pKa = 5.19LDD357 pKa = 4.29NDD359 pKa = 3.54GHH361 pKa = 8.65LDD363 pKa = 3.66VLSASYY369 pKa = 11.27YY370 pKa = 9.65DD371 pKa = 6.19DD372 pKa = 5.16KK373 pKa = 11.27IAWYY377 pKa = 9.6RR378 pKa = 11.84NKK380 pKa = 10.92GNGTFSDD387 pKa = 3.49QLIITEE393 pKa = 4.42AANGAYY399 pKa = 9.98SVFAADD405 pKa = 4.84LDD407 pKa = 4.32NNGDD411 pKa = 3.49IDD413 pKa = 4.09ILSASYY419 pKa = 10.69LGDD422 pKa = 4.37GIAWYY427 pKa = 10.71RR428 pKa = 11.84NDD430 pKa = 4.54DD431 pKa = 3.74PVAHH435 pKa = 6.97FFRR438 pKa = 11.84STT440 pKa = 2.86

Molecular weight: 47.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A9V395|A9V395_MONBE Predicted protein OS=Monosiga brevicollis OX=81824 GN=9474 PE=4 SV=1
MM1 pKa = 7.54ASKK4 pKa = 10.31AANAPAARR12 pKa = 11.84VPRR15 pKa = 11.84GAIVPRR21 pKa = 11.84LASAPTARR29 pKa = 11.84AAKK32 pKa = 9.67RR33 pKa = 11.84VTASAVATVLPLAARR48 pKa = 11.84ATARR52 pKa = 11.84TASAARR58 pKa = 11.84RR59 pKa = 11.84AATAPAAVTAPAATSATAPTASAARR84 pKa = 11.84RR85 pKa = 11.84ALRR88 pKa = 11.84PLPLAVALAANVATPVLAATSASAAKK114 pKa = 10.01IIVGTKK120 pKa = 7.58TLSVLSVRR128 pKa = 11.84LLWFTPSGTKK138 pKa = 7.39THH140 pKa = 6.95HH141 pKa = 6.35ARR143 pKa = 11.84TLASSSLFRR152 pKa = 11.84KK153 pKa = 7.88QTLFGIIQFWFGPP166 pKa = 3.74

Molecular weight: 16.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9188

0

9188

5511963

49

17829

599.9

65.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.643 ± 0.033

1.819 ± 0.019

5.836 ± 0.021

5.765 ± 0.029

3.335 ± 0.014

6.068 ± 0.03

2.801 ± 0.016

3.686 ± 0.016

3.485 ± 0.03

10.149 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.178 ± 0.013

3.401 ± 0.018

5.537 ± 0.026

4.658 ± 0.022

6.321 ± 0.028

7.752 ± 0.031

6.259 ± 0.036

6.787 ± 0.035

1.164 ± 0.009

2.355 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski