Haloechinothrix halophila YIM 93223
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 356 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W9DRV0|W9DRV0_9PSEU Protein translocase subunit SecY OS=Haloechinothrix halophila YIM 93223 OX=592678 GN=secY PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.29 RR3 pKa = 11.84 PLVRR7 pKa = 11.84 SLLVGAAMLGGSVVVSMAPAAADD30 pKa = 3.44 TAQAEE35 pKa = 4.2 QSDD38 pKa = 4.25 AVVSQSAATTKK49 pKa = 10.77 GEE51 pKa = 3.78 QSRR54 pKa = 11.84 IDD56 pKa = 4.0 SYY58 pKa = 9.51 WTDD61 pKa = 3.49 DD62 pKa = 3.45 RR63 pKa = 11.84 MEE65 pKa = 4.56 DD66 pKa = 3.43 AVPAEE71 pKa = 4.18 TRR73 pKa = 11.84 FGNRR77 pKa = 11.84 PKK79 pKa = 10.25 KK80 pKa = 10.2 DD81 pKa = 3.22 HH82 pKa = 6.57 QGPDD86 pKa = 3.37 DD87 pKa = 3.59 CRR89 pKa = 11.84 GNGRR93 pKa = 11.84 WACPDD98 pKa = 3.5 EE99 pKa = 6.01 DD100 pKa = 3.78 STATQEE106 pKa = 4.23 EE107 pKa = 4.58 PQPEE111 pKa = 4.19 LGKK114 pKa = 10.84 VFFTLSGTDD123 pKa = 3.79 YY124 pKa = 10.53 VCSGTATSSSNGDD137 pKa = 3.46 VVTTAGHH144 pKa = 6.64 CLNEE148 pKa = 4.5 GPGDD152 pKa = 3.64 FATNFAFVPAYY163 pKa = 10.12 EE164 pKa = 4.74 NGAEE168 pKa = 4.57 PYY170 pKa = 10.37 GKK172 pKa = 7.73 WTAAEE177 pKa = 4.37 LFTTEE182 pKa = 3.55 QWATAGDD189 pKa = 3.91 FEE191 pKa = 5.06 YY192 pKa = 11.05 DD193 pKa = 2.84 AGFAVMNEE201 pKa = 4.06 NASGQSLTDD210 pKa = 3.29 VVGSYY215 pKa = 10.47 PIAFNLSRR223 pKa = 11.84 GLDD226 pKa = 3.61 YY227 pKa = 11.03 KK228 pKa = 11.24 SYY230 pKa = 10.0 GYY232 pKa = 9.31 PAASPFDD239 pKa = 4.93 GEE241 pKa = 4.76 TLWSCTGTATDD252 pKa = 4.87 DD253 pKa = 4.14 PNGSSTQGIPCEE265 pKa = 4.12 MTGGSSGGGWITGGQLNSVNSYY287 pKa = 10.3 KK288 pKa = 10.6 YY289 pKa = 10.35 RR290 pKa = 11.84 GDD292 pKa = 3.63 DD293 pKa = 3.16 STMYY297 pKa = 10.56 GPYY300 pKa = 9.12 FGSVIEE306 pKa = 4.62 SVYY309 pKa = 9.3 QTASAAA315 pKa = 3.53
Molecular weight: 33.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.838
IPC2_protein 4.101
IPC_protein 4.088
Toseland 3.884
ProMoST 4.228
Dawson 4.062
Bjellqvist 4.215
Wikipedia 3.961
Rodwell 3.91
Grimsley 3.795
Solomon 4.05
Lehninger 4.012
Nozaki 4.164
DTASelect 4.38
Thurlkill 3.91
EMBOSS 3.973
Sillero 4.202
Patrickios 1.303
IPC_peptide 4.05
IPC2_peptide 4.177
IPC2.peptide.svr19 4.087
Protein with the highest isoelectric point:
>tr|W9DNK6|W9DNK6_9PSEU Uncharacterized protein OS=Haloechinothrix halophila YIM 93223 OX=592678 GN=AmyhaDRAFT_0293 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.76 KK15 pKa = 9.54 KK16 pKa = 9.72 HH17 pKa = 5.63 RR18 pKa = 11.84 KK19 pKa = 7.56 LLRR22 pKa = 11.84 RR23 pKa = 11.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 9.71 QGKK33 pKa = 8.65
Molecular weight: 4.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
356
0
356
109024
33
1341
306.2
32.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.445 ± 0.21
0.733 ± 0.035
6.378 ± 0.103
6.007 ± 0.135
2.894 ± 0.077
9.178 ± 0.142
2.019 ± 0.06
4.104 ± 0.102
2.93 ± 0.123
9.506 ± 0.174
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.978 ± 0.058
2.168 ± 0.065
5.69 ± 0.147
3.042 ± 0.097
7.277 ± 0.151
5.527 ± 0.095
6.121 ± 0.118
8.691 ± 0.122
1.259 ± 0.051
2.053 ± 0.066
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here