Haloechinothrix halophila YIM 93223

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Haloechinothrix; Haloechinothrix halophila

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 356 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|W9DRV0|W9DRV0_9PSEU Protein translocase subunit SecY OS=Haloechinothrix halophila YIM 93223 OX=592678 GN=secY PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 10.29RR3 pKa = 11.84PLVRR7 pKa = 11.84SLLVGAAMLGGSVVVSMAPAAADD30 pKa = 3.44TAQAEE35 pKa = 4.2QSDD38 pKa = 4.25AVVSQSAATTKK49 pKa = 10.77GEE51 pKa = 3.78QSRR54 pKa = 11.84IDD56 pKa = 4.0SYY58 pKa = 9.51WTDD61 pKa = 3.49DD62 pKa = 3.45RR63 pKa = 11.84MEE65 pKa = 4.56DD66 pKa = 3.43AVPAEE71 pKa = 4.18TRR73 pKa = 11.84FGNRR77 pKa = 11.84PKK79 pKa = 10.25KK80 pKa = 10.2DD81 pKa = 3.22HH82 pKa = 6.57QGPDD86 pKa = 3.37DD87 pKa = 3.59CRR89 pKa = 11.84GNGRR93 pKa = 11.84WACPDD98 pKa = 3.5EE99 pKa = 6.01DD100 pKa = 3.78STATQEE106 pKa = 4.23EE107 pKa = 4.58PQPEE111 pKa = 4.19LGKK114 pKa = 10.84VFFTLSGTDD123 pKa = 3.79YY124 pKa = 10.53VCSGTATSSSNGDD137 pKa = 3.46VVTTAGHH144 pKa = 6.64CLNEE148 pKa = 4.5GPGDD152 pKa = 3.64FATNFAFVPAYY163 pKa = 10.12EE164 pKa = 4.74NGAEE168 pKa = 4.57PYY170 pKa = 10.37GKK172 pKa = 7.73WTAAEE177 pKa = 4.37LFTTEE182 pKa = 3.55QWATAGDD189 pKa = 3.91FEE191 pKa = 5.06YY192 pKa = 11.05DD193 pKa = 2.84AGFAVMNEE201 pKa = 4.06NASGQSLTDD210 pKa = 3.29VVGSYY215 pKa = 10.47PIAFNLSRR223 pKa = 11.84GLDD226 pKa = 3.61YY227 pKa = 11.03KK228 pKa = 11.24SYY230 pKa = 10.0GYY232 pKa = 9.31PAASPFDD239 pKa = 4.93GEE241 pKa = 4.76TLWSCTGTATDD252 pKa = 4.87DD253 pKa = 4.14PNGSSTQGIPCEE265 pKa = 4.12MTGGSSGGGWITGGQLNSVNSYY287 pKa = 10.3KK288 pKa = 10.6YY289 pKa = 10.35RR290 pKa = 11.84GDD292 pKa = 3.63DD293 pKa = 3.16STMYY297 pKa = 10.56GPYY300 pKa = 9.12FGSVIEE306 pKa = 4.62SVYY309 pKa = 9.3QTASAAA315 pKa = 3.53

Molecular weight:
33.16 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|W9DNK6|W9DNK6_9PSEU Uncharacterized protein OS=Haloechinothrix halophila YIM 93223 OX=592678 GN=AmyhaDRAFT_0293 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.71QGKK33 pKa = 8.65

Molecular weight:
4.16 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

356

0

356

109024

33

1341

306.2

32.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.445 ± 0.21

0.733 ± 0.035

6.378 ± 0.103

6.007 ± 0.135

2.894 ± 0.077

9.178 ± 0.142

2.019 ± 0.06

4.104 ± 0.102

2.93 ± 0.123

9.506 ± 0.174

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.978 ± 0.058

2.168 ± 0.065

5.69 ± 0.147

3.042 ± 0.097

7.277 ± 0.151

5.527 ± 0.095

6.121 ± 0.118

8.691 ± 0.122

1.259 ± 0.051

2.053 ± 0.066

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski