Microbacterium laevaniformans

Taxonomy: cellular organisms;

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2869 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A150HFT8|A0A150HFT8_9MICO NYN domain protein OS=Microbacterium laevaniformans OX=36807 GN=Mlaev_01569 PE=4 SV=1
MM1 pKa = 7.21GTIQLSGVGRR11 pKa = 11.84SFKK14 pKa = 10.12STAAVSDD21 pKa = 3.26IDD23 pKa = 6.12LIIEE27 pKa = 4.23DD28 pKa = 3.95AAAQITEE35 pKa = 4.38STGAPSMSASCVITGGSWCMQGLFLSNGAQVLSDD69 pKa = 3.87DD70 pKa = 3.7RR71 pKa = 11.84TTIEE75 pKa = 4.51FGSDD79 pKa = 2.83AAIDD83 pKa = 3.76TVATFTEE90 pKa = 4.87MYY92 pKa = 10.06EE93 pKa = 4.53DD94 pKa = 3.87GVLTNEE100 pKa = 5.05DD101 pKa = 3.35FTSQYY106 pKa = 10.8EE107 pKa = 3.96AFAQGKK113 pKa = 5.89TAIHH117 pKa = 6.14VNSSALQGAFMMGAEE132 pKa = 4.06AGGWTLDD139 pKa = 3.26AHH141 pKa = 6.01TLPAFGDD148 pKa = 3.71QAVVPTNSGSFLAMFATDD166 pKa = 3.71PAKK169 pKa = 10.35QAAAWEE175 pKa = 4.39LMQFMTSPRR184 pKa = 11.84AYY186 pKa = 9.83EE187 pKa = 4.24IISTQIGYY195 pKa = 9.92LPLRR199 pKa = 11.84SSMADD204 pKa = 2.89EE205 pKa = 5.16GGPLYY210 pKa = 10.81DD211 pKa = 3.15WVEE214 pKa = 4.17ANPLVKK220 pKa = 10.37PNLEE224 pKa = 3.9QLDD227 pKa = 3.97ALEE230 pKa = 4.77PWVSYY235 pKa = 10.65PGDD238 pKa = 3.38SYY240 pKa = 12.03AQVDD244 pKa = 4.04QVLATAIEE252 pKa = 4.07DD253 pKa = 3.7AVFYY257 pKa = 10.67GADD260 pKa = 3.46PAATMTEE267 pKa = 3.91AAEE270 pKa = 4.07RR271 pKa = 11.84AQGLIEE277 pKa = 3.95

Molecular weight: 29.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A150HFB2|A0A150HFB2_9MICO Uncharacterized protein OS=Microbacterium laevaniformans OX=36807 GN=Mlaev_01259 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2869

0

2869

906975

29

2409

316.1

33.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.238 ± 0.067

0.509 ± 0.01

6.268 ± 0.044

5.21 ± 0.05

3.024 ± 0.025

8.841 ± 0.043

1.992 ± 0.025

4.519 ± 0.035

1.893 ± 0.032

10.028 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.785 ± 0.015

1.827 ± 0.024

5.392 ± 0.033

2.79 ± 0.022

7.525 ± 0.06

5.452 ± 0.034

6.189 ± 0.047

9.083 ± 0.046

1.516 ± 0.02

1.918 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski