Halobaculum gomorrense
Average proteome isoelectric point is 4.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3149 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M5QKD5|A0A1M5QKD5_9EURY 3-oxoacyl-[acyl-carrier protein] reductase OS=Halobaculum gomorrense OX=43928 GN=SAMN05443636_1933 PE=4 SV=1
MM1 pKa = 7.59 GIDD4 pKa = 4.45 PNFDD8 pKa = 3.82 DD9 pKa = 4.06 NRR11 pKa = 11.84 EE12 pKa = 4.18 HH13 pKa = 8.17 VGEE16 pKa = 4.12 EE17 pKa = 3.65 NGLDD21 pKa = 3.13 VWGPVDD27 pKa = 3.89 PPEE30 pKa = 4.39 KK31 pKa = 10.5 LGIHH35 pKa = 6.29 GAHH38 pKa = 6.12 VAVDD42 pKa = 3.77 YY43 pKa = 10.01 DD44 pKa = 3.37 ICIADD49 pKa = 4.36 GACLEE54 pKa = 4.2 NCPVDD59 pKa = 3.64 VFDD62 pKa = 5.23 WVDD65 pKa = 3.63 TPDD68 pKa = 3.53 HH69 pKa = 6.97 PEE71 pKa = 3.86 SEE73 pKa = 4.48 KK74 pKa = 10.81 KK75 pKa = 10.45 VEE77 pKa = 4.04 PTRR80 pKa = 11.84 EE81 pKa = 4.05 DD82 pKa = 3.22 QCIDD86 pKa = 3.44 CMLCVDD92 pKa = 4.88 ICPVDD97 pKa = 5.13 AIDD100 pKa = 3.92 VDD102 pKa = 4.11 ASRR105 pKa = 11.84 SS106 pKa = 3.56
Molecular weight: 11.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.808
IPC_protein 3.795
Toseland 3.579
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.63
Grimsley 3.49
Solomon 3.795
Lehninger 3.745
Nozaki 3.923
DTASelect 4.177
Thurlkill 3.643
EMBOSS 3.757
Sillero 3.923
Patrickios 1.036
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.831
Protein with the highest isoelectric point:
>tr|A0A1M5U3J6|A0A1M5U3J6_9EURY Sugar phosphate isomerase/epimerase OS=Halobaculum gomorrense OX=43928 GN=SAMN05443636_2907 PE=4 SV=1
MM1 pKa = 7.78 DD2 pKa = 4.19 WYY4 pKa = 11.03 RR5 pKa = 11.84 LLRR8 pKa = 11.84 SAGTRR13 pKa = 11.84 RR14 pKa = 11.84 LSVDD18 pKa = 2.86 QRR20 pKa = 11.84 ALVALRR26 pKa = 11.84 VAVGLLLLTDD36 pKa = 5.03 LLLCTRR42 pKa = 11.84 SLVFFYY48 pKa = 10.57 TDD50 pKa = 2.98 GGSLPRR56 pKa = 11.84 SALFARR62 pKa = 11.84 FPITANLSVHH72 pKa = 6.39 ALFGGAWWVGLLFVAAAVAATALALGRR99 pKa = 11.84 WTKK102 pKa = 10.34 IAAACSLVLLVSLHH116 pKa = 5.79 ARR118 pKa = 11.84 NPIALNGGDD127 pKa = 3.43 SLLRR131 pKa = 11.84 RR132 pKa = 11.84 TLLWSLFLPLGTGLTFEE149 pKa = 4.56 TRR151 pKa = 11.84 AQCRR155 pKa = 11.84 HH156 pKa = 5.78 PAAVGLLVQPIVLYY170 pKa = 10.34 VVNAVIKK177 pKa = 10.74 LRR179 pKa = 11.84 GDD181 pKa = 2.92 VWLSGEE187 pKa = 3.79 AVKK190 pKa = 10.94 YY191 pKa = 8.9 VFRR194 pKa = 11.84 IDD196 pKa = 3.08 ALTRR200 pKa = 11.84 FVGEE204 pKa = 3.96 YY205 pKa = 9.87 LAAYY209 pKa = 6.81 PTLLDD214 pKa = 3.82 ALGTAWVMLLCCSPLLVLTTGRR236 pKa = 11.84 ARR238 pKa = 11.84 ATVVAVFASAHH249 pKa = 5.35 VGMALTLGIGLFPLIGVAALIPYY272 pKa = 8.51 LPPTAWDD279 pKa = 3.44 KK280 pKa = 11.98 VEE282 pKa = 4.5 GSWNRR287 pKa = 11.84 SVKK290 pKa = 7.2 TARR293 pKa = 11.84 RR294 pKa = 11.84 VGGEE298 pKa = 3.36 RR299 pKa = 11.84 RR300 pKa = 11.84 AAFEE304 pKa = 4.37 DD305 pKa = 3.59 LWEE308 pKa = 4.41 KK309 pKa = 10.53 VCRR312 pKa = 11.84 QIGRR316 pKa = 11.84 LGRR319 pKa = 11.84 GRR321 pKa = 11.84 SLGAAPPMKK330 pKa = 10.12 PMEE333 pKa = 4.75 PLRR336 pKa = 11.84 QRR338 pKa = 11.84 IGTAGSGGRR347 pKa = 11.84 TGSTSSLVSVDD358 pKa = 3.07 RR359 pKa = 11.84 GRR361 pKa = 11.84 IATRR365 pKa = 11.84 IRR367 pKa = 11.84 RR368 pKa = 11.84 IGRR371 pKa = 11.84 AVAATMVVVVLIWNTASLGYY391 pKa = 10.4 VEE393 pKa = 5.63 VPQPEE398 pKa = 4.16 AVEE401 pKa = 4.18 VSPQEE406 pKa = 4.18 TRR408 pKa = 11.84 WDD410 pKa = 3.59 MFAPSPPTRR419 pKa = 11.84 DD420 pKa = 2.59 VWYY423 pKa = 10.04 VAVGTLEE430 pKa = 4.26 DD431 pKa = 3.96 GEE433 pKa = 4.34 RR434 pKa = 11.84 VEE436 pKa = 4.64 VIRR439 pKa = 11.84 GGEE442 pKa = 4.08 PTWARR447 pKa = 11.84 SEE449 pKa = 4.42 RR450 pKa = 11.84 RR451 pKa = 11.84 WSSYY455 pKa = 10.36 PSARR459 pKa = 11.84 WRR461 pKa = 11.84 KK462 pKa = 7.98 YY463 pKa = 10.08 LEE465 pKa = 3.95 VVRR468 pKa = 11.84 WSDD471 pKa = 3.77 DD472 pKa = 3.17 DD473 pKa = 3.37 WLQRR477 pKa = 11.84 HH478 pKa = 5.79 FASGLCARR486 pKa = 11.84 WDD488 pKa = 3.38 ATHH491 pKa = 7.45 RR492 pKa = 11.84 SRR494 pKa = 11.84 LSRR497 pKa = 11.84 VTVYY501 pKa = 10.6 VLSRR505 pKa = 11.84 SVQSSTEE512 pKa = 4.01 PNASEE517 pKa = 4.54 GPSVRR522 pKa = 11.84 RR523 pKa = 11.84 TRR525 pKa = 11.84 VRR527 pKa = 11.84 THH529 pKa = 6.05 EE530 pKa = 4.36 CSSS533 pKa = 3.28
Molecular weight: 58.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 9.648
IPC_protein 10.774
Toseland 10.613
ProMoST 10.628
Dawson 10.745
Bjellqvist 10.599
Wikipedia 11.067
Rodwell 10.672
Grimsley 10.818
Solomon 10.95
Lehninger 10.891
Nozaki 10.643
DTASelect 10.584
Thurlkill 10.657
EMBOSS 11.067
Sillero 10.701
Patrickios 10.262
IPC_peptide 10.95
IPC2_peptide 9.955
IPC2.peptide.svr19 8.594
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3149
0
3149
922802
28
2162
293.0
31.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.492 ± 0.081
0.698 ± 0.014
8.802 ± 0.057
8.083 ± 0.069
3.175 ± 0.029
9.238 ± 0.042
1.937 ± 0.02
3.401 ± 0.036
1.637 ± 0.027
8.672 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.658 ± 0.02
2.089 ± 0.026
4.778 ± 0.027
2.049 ± 0.028
6.941 ± 0.05
5.231 ± 0.03
6.177 ± 0.035
9.35 ± 0.047
1.086 ± 0.017
2.506 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here