Gordonia phage Jumbo
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B3B0P5|A0A1B3B0P5_9CAUD Uncharacterized protein OS=Gordonia phage Jumbo OX=1887650 GN=77 PE=4 SV=1
MM1 pKa = 7.64 SDD3 pKa = 3.44 EE4 pKa = 4.78 PIKK7 pKa = 10.73 FLVEE11 pKa = 3.55 VTYY14 pKa = 10.39 TPGISKK20 pKa = 10.17 GYY22 pKa = 9.2 YY23 pKa = 7.97 PEE25 pKa = 4.1 NVKK28 pKa = 9.22 TPRR31 pKa = 11.84 EE32 pKa = 3.82 AVIYY36 pKa = 9.8 DD37 pKa = 4.02 LEE39 pKa = 4.39 SEE41 pKa = 4.34 YY42 pKa = 11.45 SDD44 pKa = 4.0 FFQILDD50 pKa = 3.83 GADD53 pKa = 3.33 SLTVIEE59 pKa = 5.14 IGGEE63 pKa = 4.11 KK64 pKa = 10.07 YY65 pKa = 10.39 VEE67 pKa = 4.4 PPQEE71 pKa = 3.67
Molecular weight: 8.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.828
IPC2_protein 4.062
IPC_protein 3.923
Toseland 3.757
ProMoST 3.999
Dawson 3.872
Bjellqvist 4.113
Wikipedia 3.77
Rodwell 3.757
Grimsley 3.681
Solomon 3.859
Lehninger 3.808
Nozaki 3.999
DTASelect 4.113
Thurlkill 3.795
EMBOSS 3.783
Sillero 4.037
Patrickios 1.888
IPC_peptide 3.859
IPC2_peptide 4.012
IPC2.peptide.svr19 3.955
Protein with the highest isoelectric point:
>tr|A0A1B3B0V3|A0A1B3B0V3_9CAUD Uncharacterized protein OS=Gordonia phage Jumbo OX=1887650 GN=60 PE=4 SV=1
MM1 pKa = 7.44 ILVMTPDD8 pKa = 3.25 KK9 pKa = 10.66 TVTAMICSDD18 pKa = 4.21 RR19 pKa = 11.84 NRR21 pKa = 11.84 ADD23 pKa = 3.42 YY24 pKa = 11.3 GNKK27 pKa = 9.73 CEE29 pKa = 4.26 KK30 pKa = 10.2 NKK32 pKa = 10.75 SKK34 pKa = 10.32 IYY36 pKa = 9.33 RR37 pKa = 11.84 ARR39 pKa = 11.84 EE40 pKa = 3.53 RR41 pKa = 11.84 RR42 pKa = 11.84 EE43 pKa = 3.65 FARR46 pKa = 11.84 DD47 pKa = 3.1 TARR50 pKa = 11.84 YY51 pKa = 8.83 LSRR54 pKa = 11.84 RR55 pKa = 11.84 RR56 pKa = 3.5
Molecular weight: 6.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.663
IPC_protein 10.438
Toseland 10.584
ProMoST 10.555
Dawson 10.701
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.921
Grimsley 10.76
Solomon 10.804
Lehninger 10.774
Nozaki 10.584
DTASelect 10.409
Thurlkill 10.599
EMBOSS 10.979
Sillero 10.628
Patrickios 10.687
IPC_peptide 10.804
IPC2_peptide 9.502
IPC2.peptide.svr19 8.637
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
102
0
102
24151
42
3069
236.8
26.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.366 ± 0.795
0.927 ± 0.149
6.377 ± 0.33
6.269 ± 0.314
3.785 ± 0.181
8.157 ± 0.493
1.892 ± 0.179
5.884 ± 0.153
5.275 ± 0.263
7.155 ± 0.241
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.567 ± 0.122
4.571 ± 0.135
5.176 ± 0.325
3.673 ± 0.189
5.391 ± 0.298
6.277 ± 0.224
6.12 ± 0.166
6.401 ± 0.23
1.611 ± 0.094
3.126 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here