Amphibola crenata associated bacilladnavirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Baphyvirales; Bacilladnaviridae; Protobacilladnavirus; Snail associated protobacilladnavirus 2

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8YT78|A0A1P8YT78_9VIRU p1 OS=Amphibola crenata associated bacilladnavirus 2 OX=1941436 PE=4 SV=1
MM1 pKa = 7.17NMCRR5 pKa = 11.84PILHH9 pKa = 6.43LTAHH13 pKa = 6.06NGVRR17 pKa = 11.84EE18 pKa = 3.88IDD20 pKa = 3.5LSKK23 pKa = 11.11SLNALAMLNMTGFYY37 pKa = 10.87NDD39 pKa = 3.21ADD41 pKa = 4.03YY42 pKa = 11.69YY43 pKa = 11.05GIKK46 pKa = 10.17LWIDD50 pKa = 3.36AYY52 pKa = 10.05MQLANAPMPSSVTFKK67 pKa = 10.54SPCRR71 pKa = 11.84IEE73 pKa = 3.89PVNFITLCSDD83 pKa = 5.06LADD86 pKa = 3.06EE87 pKa = 4.22WAINEE92 pKa = 4.34GQQEE96 pKa = 4.33LQGEE100 pKa = 4.63DD101 pKa = 3.23PAIWADD107 pKa = 3.72EE108 pKa = 4.12VLSISDD114 pKa = 3.54FTVSIDD120 pKa = 4.02DD121 pKa = 4.47LGTIALEE128 pKa = 4.3DD129 pKa = 3.72VNLDD133 pKa = 3.63SFFSNDD139 pKa = 3.07VEE141 pKa = 4.52IMDD144 pKa = 4.7DD145 pKa = 3.47SSVISEE151 pKa = 3.93IVEE154 pKa = 3.88II155 pKa = 4.6

Molecular weight: 17.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8YT80|A0A1P8YT80_9VIRU Replication-associated protein OS=Amphibola crenata associated bacilladnavirus 2 OX=1941436 PE=4 SV=1
MM1 pKa = 7.61APKK4 pKa = 8.89KK5 pKa = 8.88TPSKK9 pKa = 9.62GRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84TTSVQRR20 pKa = 11.84RR21 pKa = 11.84PNPTKK26 pKa = 10.6RR27 pKa = 11.84KK28 pKa = 5.84PTKK31 pKa = 8.32KK32 pKa = 8.67TKK34 pKa = 9.95KK35 pKa = 10.3AGIAGAAAHH44 pKa = 6.46LLKK47 pKa = 10.37PLLMPFDD54 pKa = 4.17RR55 pKa = 11.84TNMQPKK61 pKa = 9.95IPDD64 pKa = 3.68GKK66 pKa = 10.22VGTSIGQKK74 pKa = 8.47FTTTRR79 pKa = 11.84EE80 pKa = 4.12HH81 pKa = 6.59FNVGAGNIMHH91 pKa = 7.5ALLFPGQSGGLVVIGCQNFNSVPISSPAFNDD122 pKa = 3.11AGGFSYY128 pKa = 8.92THH130 pKa = 7.21DD131 pKa = 4.28LASGMSTLAAKK142 pKa = 10.09EE143 pKa = 4.61NYY145 pKa = 9.44HH146 pKa = 4.99SWRR149 pKa = 11.84VVSQAARR156 pKa = 11.84FEE158 pKa = 4.39LLNAAEE164 pKa = 5.19EE165 pKa = 4.36DD166 pKa = 4.08DD167 pKa = 4.32GWWEE171 pKa = 3.93AVRR174 pKa = 11.84VTDD177 pKa = 4.25SMSVFDD183 pKa = 5.96FRR185 pKa = 11.84LDD187 pKa = 3.32QHH189 pKa = 7.34DD190 pKa = 4.04NGTGRR195 pKa = 11.84GNGTILPYY203 pKa = 9.47YY204 pKa = 9.61TLANYY209 pKa = 7.7QFKK212 pKa = 10.54PITDD216 pKa = 3.21EE217 pKa = 3.75MTYY220 pKa = 10.87EE221 pKa = 4.04SGRR224 pKa = 11.84LKK226 pKa = 10.56DD227 pKa = 3.2LHH229 pKa = 7.34KK230 pKa = 10.87RR231 pKa = 11.84EE232 pKa = 4.21FQLHH236 pKa = 4.06QVKK239 pKa = 11.0DD240 pKa = 3.92EE241 pKa = 4.09VDD243 pKa = 4.03FTQLCTPVTIEE254 pKa = 4.28GGVEE258 pKa = 3.77SGIGIVGSPYY268 pKa = 9.84VVDD271 pKa = 3.58WNGTGGANNGEE282 pKa = 4.21RR283 pKa = 11.84YY284 pKa = 10.31GMQNYY289 pKa = 9.14NAIRR293 pKa = 11.84NYY295 pKa = 10.04IDD297 pKa = 2.87TGYY300 pKa = 11.45DD301 pKa = 2.94MIYY304 pKa = 10.76FRR306 pKa = 11.84FHH308 pKa = 6.3CRR310 pKa = 11.84AAATPTRR317 pKa = 11.84LLTHH321 pKa = 6.45IVSNQEE327 pKa = 3.54IQFSSDD333 pKa = 2.72AGEE336 pKa = 4.38SRR338 pKa = 11.84FQTLSARR345 pKa = 11.84VPQAAATLAARR356 pKa = 11.84ANGGKK361 pKa = 7.52TASVPAQGKK370 pKa = 9.79DD371 pKa = 3.35PSRR374 pKa = 11.84HH375 pKa = 5.34GGG377 pKa = 3.25

Molecular weight: 41.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1242

155

532

310.5

35.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.683 ± 1.189

1.369 ± 0.393

6.441 ± 0.963

6.441 ± 0.898

4.267 ± 0.455

7.246 ± 1.115

2.979 ± 0.548

6.039 ± 0.906

7.166 ± 1.796

6.039 ± 0.841

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.496 ± 0.392

4.348 ± 0.726

4.992 ± 0.199

3.301 ± 0.469

5.878 ± 0.745

7.085 ± 0.454

7.005 ± 0.597

5.314 ± 0.165

1.771 ± 0.437

3.14 ± 0.258
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski