Parvibium lacunae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Parvibium

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2432 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A368L4U0|A0A368L4U0_9BURK Enoyl-CoA hydratase/isomerase family protein OS=Parvibium lacunae OX=1888893 GN=DU000_07220 PE=3 SV=1
MM1 pKa = 6.9KK2 pKa = 9.6TWMCLICGWIYY13 pKa = 11.27DD14 pKa = 4.08EE15 pKa = 4.98AQGSPEE21 pKa = 3.93EE22 pKa = 4.89GIPAGTRR29 pKa = 11.84WEE31 pKa = 4.19DD32 pKa = 3.48VPPNWTCPEE41 pKa = 3.92CGARR45 pKa = 11.84KK46 pKa = 9.51EE47 pKa = 3.97DD48 pKa = 3.81FEE50 pKa = 4.37MVEE53 pKa = 4.0II54 pKa = 4.86

Molecular weight: 6.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A368L0R2|A0A368L0R2_9BURK TMEM189_B_dmain domain-containing protein OS=Parvibium lacunae OX=1888893 GN=DU000_10000 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.6RR14 pKa = 11.84THH16 pKa = 5.71GFLVRR21 pKa = 11.84SRR23 pKa = 11.84TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84HH40 pKa = 4.92RR41 pKa = 11.84LAVV44 pKa = 3.37

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2432

0

2432

799854

37

3585

328.9

36.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.376 ± 0.07

0.978 ± 0.016

4.685 ± 0.043

5.347 ± 0.046

3.543 ± 0.033

7.163 ± 0.06

2.337 ± 0.029

5.321 ± 0.04

3.818 ± 0.045

11.34 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.24 ± 0.025

3.294 ± 0.037

5.036 ± 0.04

5.563 ± 0.056

5.88 ± 0.046

5.551 ± 0.04

5.529 ± 0.04

6.838 ± 0.045

1.479 ± 0.025

2.682 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski