Amino acid dipepetide frequency for Streptococcus satellite phage Javan250

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.797AlaAla: 0.797 ± 0.452
0.266AlaCys: 0.266 ± 0.285
7.177AlaAsp: 7.177 ± 1.418
3.987AlaGlu: 3.987 ± 1.052
3.19AlaPhe: 3.19 ± 0.845
2.127AlaGly: 2.127 ± 0.629
0.0AlaHis: 0.0 ± 0.0
4.785AlaIle: 4.785 ± 0.942
6.911AlaLys: 6.911 ± 1.21
6.114AlaLeu: 6.114 ± 1.136
3.19AlaMet: 3.19 ± 0.917
2.658AlaAsn: 2.658 ± 0.865
0.797AlaPro: 0.797 ± 0.361
1.595AlaGln: 1.595 ± 0.615
3.456AlaArg: 3.456 ± 0.978
4.785AlaSer: 4.785 ± 1.074
5.051AlaThr: 5.051 ± 1.04
1.861AlaVal: 1.861 ± 0.684
0.266AlaTrp: 0.266 ± 0.222
2.658AlaTyr: 2.658 ± 0.784
0.0AlaXaa: 0.0 ± 0.0
Cys
0.0CysAla: 0.0 ± 0.0
0.0CysCys: 0.0 ± 0.0
0.532CysAsp: 0.532 ± 0.4
0.0CysGlu: 0.0 ± 0.0
0.0CysPhe: 0.0 ± 0.0
0.0CysGly: 0.0 ± 0.0
0.0CysHis: 0.0 ± 0.0
0.532CysIle: 0.532 ± 0.31
0.266CysLys: 0.266 ± 0.307
0.532CysLeu: 0.532 ± 0.313
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.0CysPro: 0.0 ± 0.0
0.532CysGln: 0.532 ± 0.349
0.266CysArg: 0.266 ± 0.228
0.266CysSer: 0.266 ± 0.321
0.0CysThr: 0.0 ± 0.0
0.0CysVal: 0.0 ± 0.0
0.0CysTrp: 0.0 ± 0.0
0.797CysTyr: 0.797 ± 0.649
0.0CysXaa: 0.0 ± 0.0
Asp
1.595AspAla: 1.595 ± 0.709
0.532AspCys: 0.532 ± 0.381
5.051AspAsp: 5.051 ± 0.991
4.785AspGlu: 4.785 ± 1.229
4.519AspPhe: 4.519 ± 0.768
3.456AspGly: 3.456 ± 1.167
1.063AspHis: 1.063 ± 0.431
8.772AspIle: 8.772 ± 2.209
5.848AspLys: 5.848 ± 1.302
6.114AspLeu: 6.114 ± 1.056
1.595AspMet: 1.595 ± 0.53
3.721AspAsn: 3.721 ± 0.85
1.063AspPro: 1.063 ± 0.547
1.063AspGln: 1.063 ± 0.465
3.456AspArg: 3.456 ± 0.935
2.392AspSer: 2.392 ± 0.671
3.19AspThr: 3.19 ± 0.643
4.519AspVal: 4.519 ± 1.118
0.797AspTrp: 0.797 ± 0.397
5.582AspTyr: 5.582 ± 1.071
0.0AspXaa: 0.0 ± 0.0
Glu
7.177GluAla: 7.177 ± 2.055
0.266GluCys: 0.266 ± 0.285
3.456GluAsp: 3.456 ± 0.996
4.519GluGlu: 4.519 ± 1.468
2.658GluPhe: 2.658 ± 0.932
3.456GluGly: 3.456 ± 1.318
1.861GluHis: 1.861 ± 0.715
7.709GluIle: 7.709 ± 1.626
4.785GluLys: 4.785 ± 1.144
11.164GluLeu: 11.164 ± 1.533
2.392GluMet: 2.392 ± 0.783
6.114GluAsn: 6.114 ± 1.528
1.329GluPro: 1.329 ± 0.607
2.658GluGln: 2.658 ± 0.652
4.519GluArg: 4.519 ± 1.294
3.987GluSer: 3.987 ± 0.998
3.456GluThr: 3.456 ± 0.958
3.19GluVal: 3.19 ± 0.627
0.532GluTrp: 0.532 ± 0.313
3.987GluTyr: 3.987 ± 0.926
0.0GluXaa: 0.0 ± 0.0
Phe
2.924PheAla: 2.924 ± 0.784
0.0PheCys: 0.0 ± 0.0
2.392PheAsp: 2.392 ± 1.194
5.051PheGlu: 5.051 ± 0.835
1.595PhePhe: 1.595 ± 0.528
1.861PheGly: 1.861 ± 0.702
0.797PheHis: 0.797 ± 0.365
1.861PheIle: 1.861 ± 0.682
2.924PheLys: 2.924 ± 1.012
3.721PheLeu: 3.721 ± 0.719
0.266PheMet: 0.266 ± 0.304
4.519PheAsn: 4.519 ± 1.175
0.266PhePro: 0.266 ± 0.249
0.797PheGln: 0.797 ± 0.452
2.658PheArg: 2.658 ± 0.545
2.924PheSer: 2.924 ± 0.92
2.392PheThr: 2.392 ± 0.79
1.595PheVal: 1.595 ± 0.688
0.0PheTrp: 0.0 ± 0.0
2.392PheTyr: 2.392 ± 0.708
0.0PheXaa: 0.0 ± 0.0
Gly
2.392GlyAla: 2.392 ± 0.82
0.532GlyCys: 0.532 ± 0.317
1.595GlyAsp: 1.595 ± 0.729
3.19GlyGlu: 3.19 ± 0.875
2.392GlyPhe: 2.392 ± 1.104
1.595GlyGly: 1.595 ± 0.518
0.797GlyHis: 0.797 ± 0.395
2.392GlyIle: 2.392 ± 0.753
4.253GlyLys: 4.253 ± 0.879
4.785GlyLeu: 4.785 ± 1.146
2.127GlyMet: 2.127 ± 0.577
1.329GlyAsn: 1.329 ± 0.594
0.0GlyPro: 0.0 ± 0.0
1.595GlyGln: 1.595 ± 0.688
3.721GlyArg: 3.721 ± 0.902
2.127GlySer: 2.127 ± 0.738
2.127GlyThr: 2.127 ± 0.766
6.911GlyVal: 6.911 ± 1.317
0.532GlyTrp: 0.532 ± 0.429
2.658GlyTyr: 2.658 ± 0.837
0.0GlyXaa: 0.0 ± 0.0
His
0.797HisAla: 0.797 ± 0.501
0.0HisCys: 0.0 ± 0.0
1.861HisAsp: 1.861 ± 0.752
0.797HisGlu: 0.797 ± 0.369
0.532HisPhe: 0.532 ± 0.414
1.595HisGly: 1.595 ± 0.629
0.266HisHis: 0.266 ± 0.305
1.861HisIle: 1.861 ± 0.531
1.595HisLys: 1.595 ± 0.707
1.595HisLeu: 1.595 ± 0.565
0.0HisMet: 0.0 ± 0.0
1.063HisAsn: 1.063 ± 0.417
0.0HisPro: 0.0 ± 0.0
0.797HisGln: 0.797 ± 0.461
0.532HisArg: 0.532 ± 0.331
1.063HisSer: 1.063 ± 0.415
0.797HisThr: 0.797 ± 0.358
0.797HisVal: 0.797 ± 0.425
0.0HisTrp: 0.0 ± 0.0
0.532HisTyr: 0.532 ± 0.312
0.0HisXaa: 0.0 ± 0.0
Ile
5.582IleAla: 5.582 ± 1.128
0.266IleCys: 0.266 ± 0.307
7.974IleAsp: 7.974 ± 1.311
7.443IleGlu: 7.443 ± 1.306
2.127IlePhe: 2.127 ± 0.709
2.924IleGly: 2.924 ± 0.643
0.797IleHis: 0.797 ± 0.501
3.456IleIle: 3.456 ± 0.89
5.582IleLys: 5.582 ± 1.136
2.924IleLeu: 2.924 ± 0.819
2.127IleMet: 2.127 ± 0.686
5.316IleAsn: 5.316 ± 1.182
2.658IlePro: 2.658 ± 0.707
2.924IleGln: 2.924 ± 0.859
3.987IleArg: 3.987 ± 1.007
7.443IleSer: 7.443 ± 1.875
3.456IleThr: 3.456 ± 0.864
3.19IleVal: 3.19 ± 1.137
1.063IleTrp: 1.063 ± 0.62
2.658IleTyr: 2.658 ± 0.845
0.0IleXaa: 0.0 ± 0.0
Lys
6.38LysAla: 6.38 ± 1.161
0.266LysCys: 0.266 ± 0.273
5.316LysAsp: 5.316 ± 1.134
8.24LysGlu: 8.24 ± 1.675
1.063LysPhe: 1.063 ± 0.528
3.987LysGly: 3.987 ± 0.702
2.658LysHis: 2.658 ± 0.505
6.911LysIle: 6.911 ± 1.475
7.177LysLys: 7.177 ± 1.404
7.443LysLeu: 7.443 ± 1.336
2.392LysMet: 2.392 ± 0.677
5.582LysAsn: 5.582 ± 1.178
2.924LysPro: 2.924 ± 0.873
4.785LysGln: 4.785 ± 1.193
3.19LysArg: 3.19 ± 0.985
5.582LysSer: 5.582 ± 0.991
6.38LysThr: 6.38 ± 1.278
5.316LysVal: 5.316 ± 1.459
0.532LysTrp: 0.532 ± 0.395
1.063LysTyr: 1.063 ± 0.452
0.0LysXaa: 0.0 ± 0.0
Leu
6.38LeuAla: 6.38 ± 1.251
0.532LeuCys: 0.532 ± 0.314
6.38LeuAsp: 6.38 ± 1.064
9.835LeuGlu: 9.835 ± 1.675
4.253LeuPhe: 4.253 ± 1.272
5.316LeuGly: 5.316 ± 1.11
1.063LeuHis: 1.063 ± 0.496
6.38LeuIle: 6.38 ± 1.441
9.835LeuLys: 9.835 ± 1.402
9.835LeuLeu: 9.835 ± 1.434
1.329LeuMet: 1.329 ± 0.642
4.253LeuAsn: 4.253 ± 1.104
1.063LeuPro: 1.063 ± 0.494
4.253LeuGln: 4.253 ± 0.875
5.051LeuArg: 5.051 ± 1.2
6.114LeuSer: 6.114 ± 1.507
8.24LeuThr: 8.24 ± 1.201
4.519LeuVal: 4.519 ± 0.959
0.532LeuTrp: 0.532 ± 0.39
3.721LeuTyr: 3.721 ± 0.91
0.0LeuXaa: 0.0 ± 0.0
Met
2.924MetAla: 2.924 ± 0.923
0.0MetCys: 0.0 ± 0.0
0.797MetAsp: 0.797 ± 0.421
1.595MetGlu: 1.595 ± 0.721
0.532MetPhe: 0.532 ± 0.316
0.797MetGly: 0.797 ± 0.42
0.0MetHis: 0.0 ± 0.0
2.392MetIle: 2.392 ± 0.848
1.595MetLys: 1.595 ± 0.582
2.658MetLeu: 2.658 ± 0.997
0.797MetMet: 0.797 ± 0.42
1.329MetAsn: 1.329 ± 0.429
0.0MetPro: 0.0 ± 0.0
0.532MetGln: 0.532 ± 0.431
1.063MetArg: 1.063 ± 0.653
0.532MetSer: 0.532 ± 0.399
5.582MetThr: 5.582 ± 1.158
1.595MetVal: 1.595 ± 0.663
0.266MetTrp: 0.266 ± 0.291
0.266MetTyr: 0.266 ± 0.222
0.0MetXaa: 0.0 ± 0.0
Asn
4.253AsnAla: 4.253 ± 1.134
0.266AsnCys: 0.266 ± 0.321
4.519AsnAsp: 4.519 ± 1.097
4.519AsnGlu: 4.519 ± 0.821
2.127AsnPhe: 2.127 ± 0.554
3.721AsnGly: 3.721 ± 0.767
1.329AsnHis: 1.329 ± 0.935
3.721AsnIle: 3.721 ± 0.994
5.051AsnLys: 5.051 ± 1.156
5.582AsnLeu: 5.582 ± 1.454
1.329AsnMet: 1.329 ± 0.604
5.582AsnAsn: 5.582 ± 1.41
1.329AsnPro: 1.329 ± 0.534
2.924AsnGln: 2.924 ± 0.878
1.595AsnArg: 1.595 ± 0.765
3.456AsnSer: 3.456 ± 1.133
2.658AsnThr: 2.658 ± 0.913
1.329AsnVal: 1.329 ± 0.49
1.063AsnTrp: 1.063 ± 0.492
2.392AsnTyr: 2.392 ± 0.595
0.0AsnXaa: 0.0 ± 0.0
Pro
1.063ProAla: 1.063 ± 0.397
0.0ProCys: 0.0 ± 0.0
1.329ProAsp: 1.329 ± 0.785
1.595ProGlu: 1.595 ± 0.565
1.595ProPhe: 1.595 ± 0.542
0.266ProGly: 0.266 ± 0.305
0.532ProHis: 0.532 ± 0.349
0.797ProIle: 0.797 ± 0.657
2.658ProLys: 2.658 ± 0.764
1.595ProLeu: 1.595 ± 0.633
0.797ProMet: 0.797 ± 0.429
1.329ProAsn: 1.329 ± 0.74
0.266ProPro: 0.266 ± 0.304
0.266ProGln: 0.266 ± 0.265
0.797ProArg: 0.797 ± 0.409
1.595ProSer: 1.595 ± 0.723
1.861ProThr: 1.861 ± 0.736
1.595ProVal: 1.595 ± 0.779
0.0ProTrp: 0.0 ± 0.0
1.595ProTyr: 1.595 ± 0.598
0.0ProXaa: 0.0 ± 0.0
Gln
2.924GlnAla: 2.924 ± 0.855
0.0GlnCys: 0.0 ± 0.0
0.797GlnAsp: 0.797 ± 0.501
4.519GlnGlu: 4.519 ± 0.901
1.329GlnPhe: 1.329 ± 0.582
2.658GlnGly: 2.658 ± 0.692
0.797GlnHis: 0.797 ± 0.377
3.19GlnIle: 3.19 ± 0.917
2.924GlnLys: 2.924 ± 0.934
3.456GlnLeu: 3.456 ± 0.958
0.266GlnMet: 0.266 ± 0.253
1.329GlnAsn: 1.329 ± 0.667
0.266GlnPro: 0.266 ± 0.304
1.063GlnGln: 1.063 ± 0.471
1.329GlnArg: 1.329 ± 0.536
2.924GlnSer: 2.924 ± 0.943
3.19GlnThr: 3.19 ± 0.859
2.924GlnVal: 2.924 ± 0.868
0.266GlnTrp: 0.266 ± 0.265
1.329GlnTyr: 1.329 ± 0.557
0.0GlnXaa: 0.0 ± 0.0
Arg
2.658ArgAla: 2.658 ± 0.741
0.266ArgCys: 0.266 ± 0.283
2.924ArgAsp: 2.924 ± 1.123
3.721ArgGlu: 3.721 ± 0.872
1.861ArgPhe: 1.861 ± 0.715
2.127ArgGly: 2.127 ± 0.573
0.532ArgHis: 0.532 ± 0.317
2.392ArgIle: 2.392 ± 0.707
5.316ArgLys: 5.316 ± 1.228
7.443ArgLeu: 7.443 ± 1.486
1.595ArgMet: 1.595 ± 0.785
1.329ArgAsn: 1.329 ± 0.465
1.861ArgPro: 1.861 ± 0.658
1.595ArgGln: 1.595 ± 0.619
1.861ArgArg: 1.861 ± 0.507
2.924ArgSer: 2.924 ± 0.976
2.924ArgThr: 2.924 ± 0.845
2.392ArgVal: 2.392 ± 0.699
0.532ArgTrp: 0.532 ± 0.418
1.329ArgTyr: 1.329 ± 0.558
0.0ArgXaa: 0.0 ± 0.0
Ser
2.924SerAla: 2.924 ± 0.75
0.0SerCys: 0.0 ± 0.0
4.519SerAsp: 4.519 ± 0.756
6.114SerGlu: 6.114 ± 1.172
2.127SerPhe: 2.127 ± 0.98
2.924SerGly: 2.924 ± 1.28
1.063SerHis: 1.063 ± 0.501
3.721SerIle: 3.721 ± 1.399
7.177SerLys: 7.177 ± 1.419
5.316SerLeu: 5.316 ± 0.993
1.063SerMet: 1.063 ± 0.438
2.392SerAsn: 2.392 ± 0.814
2.392SerPro: 2.392 ± 0.609
3.456SerGln: 3.456 ± 1.153
2.658SerArg: 2.658 ± 0.785
3.19SerSer: 3.19 ± 1.305
3.19SerThr: 3.19 ± 1.037
4.253SerVal: 4.253 ± 0.892
0.266SerTrp: 0.266 ± 0.249
3.987SerTyr: 3.987 ± 0.891
0.0SerXaa: 0.0 ± 0.0
Thr
5.316ThrAla: 5.316 ± 1.318
0.0ThrCys: 0.0 ± 0.0
5.316ThrAsp: 5.316 ± 1.309
3.721ThrGlu: 3.721 ± 0.963
3.456ThrPhe: 3.456 ± 0.975
2.658ThrGly: 2.658 ± 0.856
0.532ThrHis: 0.532 ± 0.317
5.848ThrIle: 5.848 ± 1.138
4.253ThrLys: 4.253 ± 0.955
5.848ThrLeu: 5.848 ± 1.303
1.595ThrMet: 1.595 ± 0.674
3.987ThrAsn: 3.987 ± 1.057
2.658ThrPro: 2.658 ± 0.902
2.658ThrGln: 2.658 ± 1.255
3.19ThrArg: 3.19 ± 0.807
3.19ThrSer: 3.19 ± 0.737
3.721ThrThr: 3.721 ± 0.987
2.924ThrVal: 2.924 ± 0.958
0.532ThrTrp: 0.532 ± 0.409
1.595ThrTyr: 1.595 ± 0.944
0.0ThrXaa: 0.0 ± 0.0
Val
2.127ValAla: 2.127 ± 0.646
0.0ValCys: 0.0 ± 0.0
3.456ValAsp: 3.456 ± 0.93
3.456ValGlu: 3.456 ± 1.314
1.861ValPhe: 1.861 ± 0.809
2.658ValGly: 2.658 ± 0.735
0.797ValHis: 0.797 ± 0.404
3.19ValIle: 3.19 ± 0.901
4.253ValLys: 4.253 ± 1.285
6.38ValLeu: 6.38 ± 1.561
1.329ValMet: 1.329 ± 0.482
4.785ValAsn: 4.785 ± 1.015
2.127ValPro: 2.127 ± 0.593
2.127ValGln: 2.127 ± 0.624
1.329ValArg: 1.329 ± 0.583
5.316ValSer: 5.316 ± 0.905
2.924ValThr: 2.924 ± 0.961
3.987ValVal: 3.987 ± 1.284
0.266ValTrp: 0.266 ± 0.273
2.127ValTyr: 2.127 ± 0.754
0.0ValXaa: 0.0 ± 0.0
Trp
1.861TrpAla: 1.861 ± 0.608
0.0TrpCys: 0.0 ± 0.0
0.266TrpAsp: 0.266 ± 0.305
0.797TrpGlu: 0.797 ± 0.386
0.266TrpPhe: 0.266 ± 0.321
0.0TrpGly: 0.0 ± 0.0
0.0TrpHis: 0.0 ± 0.0
0.532TrpIle: 0.532 ± 0.422
1.063TrpLys: 1.063 ± 0.501
1.063TrpLeu: 1.063 ± 0.549
0.0TrpMet: 0.0 ± 0.0
0.266TrpAsn: 0.266 ± 0.265
0.0TrpPro: 0.0 ± 0.0
0.266TrpGln: 0.266 ± 0.283
0.266TrpArg: 0.266 ± 0.228
0.532TrpSer: 0.532 ± 0.353
0.0TrpThr: 0.0 ± 0.0
0.532TrpVal: 0.532 ± 0.391
0.266TrpTrp: 0.266 ± 0.228
0.0TrpTyr: 0.0 ± 0.0
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.595TyrAla: 1.595 ± 0.65
0.532TyrCys: 0.532 ± 0.355
3.456TyrAsp: 3.456 ± 0.928
1.329TyrGlu: 1.329 ± 0.689
3.456TyrPhe: 3.456 ± 0.828
2.924TyrGly: 2.924 ± 0.762
1.595TyrHis: 1.595 ± 0.627
3.456TyrIle: 3.456 ± 0.804
3.721TyrLys: 3.721 ± 1.198
5.582TyrLeu: 5.582 ± 1.107
0.797TyrMet: 0.797 ± 0.422
1.861TyrAsn: 1.861 ± 0.745
0.532TyrPro: 0.532 ± 0.291
1.595TyrGln: 1.595 ± 0.587
2.658TyrArg: 2.658 ± 1.054
2.392TyrSer: 2.392 ± 0.81
1.861TyrThr: 1.861 ± 0.648
1.063TyrVal: 1.063 ± 0.538
0.266TyrTrp: 0.266 ± 0.222
2.392TyrTyr: 2.392 ± 0.635
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 25 proteins (3763 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski