Amino acid dipepetide frequency for Klebsiella phage KPN5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.715AlaAla: 5.715 ± 0.374
0.411AlaCys: 0.411 ± 0.108
4.687AlaAsp: 4.687 ± 0.282
4.893AlaGlu: 4.893 ± 0.324
2.96AlaPhe: 2.96 ± 0.27
5.14AlaGly: 5.14 ± 0.495
1.275AlaHis: 1.275 ± 0.176
5.119AlaIle: 5.119 ± 0.309
5.386AlaLys: 5.386 ± 0.333
5.88AlaLeu: 5.88 ± 0.398
1.727AlaMet: 1.727 ± 0.203
3.721AlaAsn: 3.721 ± 0.283
2.611AlaPro: 2.611 ± 0.269
2.837AlaGln: 2.837 ± 0.277
3.413AlaArg: 3.413 ± 0.24
4.461AlaSer: 4.461 ± 0.318
3.721AlaThr: 3.721 ± 0.436
4.461AlaVal: 4.461 ± 0.322
1.069AlaTrp: 1.069 ± 0.158
2.878AlaTyr: 2.878 ± 0.254
0.021AlaXaa: 0.021 ± 0.018
Cys
0.699CysAla: 0.699 ± 0.117
0.206CysCys: 0.206 ± 0.063
0.884CysAsp: 0.884 ± 0.148
0.617CysGlu: 0.617 ± 0.118
0.247CysPhe: 0.247 ± 0.059
0.781CysGly: 0.781 ± 0.151
0.185CysHis: 0.185 ± 0.061
0.535CysIle: 0.535 ± 0.105
0.781CysLys: 0.781 ± 0.111
0.699CysLeu: 0.699 ± 0.129
0.349CysMet: 0.349 ± 0.083
0.391CysAsn: 0.391 ± 0.092
0.452CysPro: 0.452 ± 0.095
0.411CysGln: 0.411 ± 0.095
0.617CysArg: 0.617 ± 0.111
0.596CysSer: 0.596 ± 0.119
0.514CysThr: 0.514 ± 0.11
0.843CysVal: 0.843 ± 0.136
0.206CysTrp: 0.206 ± 0.065
0.452CysTyr: 0.452 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
4.975AspAla: 4.975 ± 0.317
0.555AspCys: 0.555 ± 0.119
4.235AspAsp: 4.235 ± 0.351
4.317AspGlu: 4.317 ± 0.379
3.31AspPhe: 3.31 ± 0.265
5.325AspGly: 5.325 ± 0.296
0.72AspHis: 0.72 ± 0.109
5.16AspIle: 5.16 ± 0.291
4.831AspLys: 4.831 ± 0.317
4.955AspLeu: 4.955 ± 0.332
2.056AspMet: 2.056 ± 0.231
3.228AspAsn: 3.228 ± 0.243
2.076AspPro: 2.076 ± 0.208
1.48AspGln: 1.48 ± 0.181
2.138AspArg: 2.138 ± 0.244
4.256AspSer: 4.256 ± 0.258
3.248AspThr: 3.248 ± 0.308
3.68AspVal: 3.68 ± 0.237
1.234AspTrp: 1.234 ± 0.159
2.899AspTyr: 2.899 ± 0.258
0.0AspXaa: 0.0 ± 0.0
Glu
5.325GluAla: 5.325 ± 0.387
0.925GluCys: 0.925 ± 0.154
4.214GluAsp: 4.214 ± 0.366
5.283GluGlu: 5.283 ± 0.424
3.125GluPhe: 3.125 ± 0.246
3.947GluGly: 3.947 ± 0.333
1.192GluHis: 1.192 ± 0.161
5.14GluIle: 5.14 ± 0.332
4.214GluLys: 4.214 ± 0.35
6.743GluLeu: 6.743 ± 0.447
2.282GluMet: 2.282 ± 0.205
3.495GluAsn: 3.495 ± 0.244
1.994GluPro: 1.994 ± 0.21
2.241GluGln: 2.241 ± 0.22
2.94GluArg: 2.94 ± 0.299
3.906GluSer: 3.906 ± 0.271
3.988GluThr: 3.988 ± 0.266
4.934GluVal: 4.934 ± 0.296
1.254GluTrp: 1.254 ± 0.165
3.31GluTyr: 3.31 ± 0.272
0.0GluXaa: 0.0 ± 0.0
Phe
2.816PheAla: 2.816 ± 0.274
0.576PheCys: 0.576 ± 0.121
2.981PheAsp: 2.981 ± 0.209
3.68PheGlu: 3.68 ± 0.277
1.275PhePhe: 1.275 ± 0.161
2.96PheGly: 2.96 ± 0.245
0.637PheHis: 0.637 ± 0.117
2.858PheIle: 2.858 ± 0.255
3.639PheLys: 3.639 ± 0.346
2.035PheLeu: 2.035 ± 0.186
1.419PheMet: 1.419 ± 0.166
2.59PheAsn: 2.59 ± 0.214
1.213PhePro: 1.213 ± 0.139
1.172PheGln: 1.172 ± 0.13
1.706PheArg: 1.706 ± 0.153
3.269PheSer: 3.269 ± 0.238
2.755PheThr: 2.755 ± 0.216
2.837PheVal: 2.837 ± 0.217
0.74PheTrp: 0.74 ± 0.129
1.583PheTyr: 1.583 ± 0.21
0.0PheXaa: 0.0 ± 0.0
Gly
4.358GlyAla: 4.358 ± 0.413
0.576GlyCys: 0.576 ± 0.118
4.276GlyAsp: 4.276 ± 0.41
4.132GlyGlu: 4.132 ± 0.235
2.488GlyPhe: 2.488 ± 0.254
3.742GlyGly: 3.742 ± 0.397
1.09GlyHis: 1.09 ± 0.193
4.071GlyIle: 4.071 ± 0.275
5.037GlyLys: 5.037 ± 0.286
5.283GlyLeu: 5.283 ± 0.32
1.932GlyMet: 1.932 ± 0.214
3.598GlyAsn: 3.598 ± 0.412
1.604GlyPro: 1.604 ± 0.179
2.385GlyGln: 2.385 ± 0.237
2.693GlyArg: 2.693 ± 0.266
3.968GlySer: 3.968 ± 0.302
4.441GlyThr: 4.441 ± 0.472
4.42GlyVal: 4.42 ± 0.316
0.946GlyTrp: 0.946 ± 0.13
3.043GlyTyr: 3.043 ± 0.221
0.0GlyXaa: 0.0 ± 0.0
His
0.884HisAla: 0.884 ± 0.128
0.226HisCys: 0.226 ± 0.066
1.131HisAsp: 1.131 ± 0.143
1.295HisGlu: 1.295 ± 0.18
0.863HisPhe: 0.863 ± 0.135
1.172HisGly: 1.172 ± 0.165
0.206HisHis: 0.206 ± 0.065
1.398HisIle: 1.398 ± 0.157
1.336HisLys: 1.336 ± 0.158
1.48HisLeu: 1.48 ± 0.196
0.37HisMet: 0.37 ± 0.109
0.678HisAsn: 0.678 ± 0.131
1.069HisPro: 1.069 ± 0.145
0.493HisGln: 0.493 ± 0.102
0.699HisArg: 0.699 ± 0.124
0.946HisSer: 0.946 ± 0.129
0.863HisThr: 0.863 ± 0.133
1.275HisVal: 1.275 ± 0.128
0.288HisTrp: 0.288 ± 0.084
0.699HisTyr: 0.699 ± 0.115
0.0HisXaa: 0.0 ± 0.0
Ile
4.399IleAla: 4.399 ± 0.332
0.863IleCys: 0.863 ± 0.121
4.852IleAsp: 4.852 ± 0.339
5.098IleGlu: 5.098 ± 0.336
2.405IlePhe: 2.405 ± 0.196
3.577IleGly: 3.577 ± 0.247
1.295IleHis: 1.295 ± 0.155
4.564IleIle: 4.564 ± 0.348
5.982IleLys: 5.982 ± 0.424
3.865IleLeu: 3.865 ± 0.293
2.159IleMet: 2.159 ± 0.206
4.338IleAsn: 4.338 ± 0.305
2.57IlePro: 2.57 ± 0.224
2.323IleGln: 2.323 ± 0.212
3.372IleArg: 3.372 ± 0.27
4.029IleSer: 4.029 ± 0.284
4.749IleThr: 4.749 ± 0.3
4.913IleVal: 4.913 ± 0.315
0.72IleTrp: 0.72 ± 0.145
2.138IleTyr: 2.138 ± 0.172
0.0IleXaa: 0.0 ± 0.0
Lys
6.044LysAla: 6.044 ± 0.389
0.802LysCys: 0.802 ± 0.137
4.872LysAsp: 4.872 ± 0.312
5.53LysGlu: 5.53 ± 0.384
3.988LysPhe: 3.988 ± 0.317
4.112LysGly: 4.112 ± 0.274
1.686LysHis: 1.686 ± 0.172
4.749LysIle: 4.749 ± 0.279
4.153LysLys: 4.153 ± 0.335
5.797LysLeu: 5.797 ± 0.341
2.693LysMet: 2.693 ± 0.236
3.454LysAsn: 3.454 ± 0.23
2.22LysPro: 2.22 ± 0.2
2.611LysGln: 2.611 ± 0.227
3.515LysArg: 3.515 ± 0.27
4.585LysSer: 4.585 ± 0.39
4.379LysThr: 4.379 ± 0.272
5.016LysVal: 5.016 ± 0.364
0.966LysTrp: 0.966 ± 0.147
3.104LysTyr: 3.104 ± 0.213
0.0LysXaa: 0.0 ± 0.0
Leu
5.427LeuAla: 5.427 ± 0.388
0.637LeuCys: 0.637 ± 0.138
4.955LeuAsp: 4.955 ± 0.347
5.633LeuGlu: 5.633 ± 0.388
2.673LeuPhe: 2.673 ± 0.228
4.256LeuGly: 4.256 ± 0.245
1.295LeuHis: 1.295 ± 0.147
4.996LeuIle: 4.996 ± 0.361
5.633LeuLys: 5.633 ± 0.373
4.728LeuLeu: 4.728 ± 0.309
2.385LeuMet: 2.385 ± 0.228
4.441LeuAsn: 4.441 ± 0.274
3.063LeuPro: 3.063 ± 0.254
2.508LeuGln: 2.508 ± 0.278
3.659LeuArg: 3.659 ± 0.285
4.543LeuSer: 4.543 ± 0.356
4.441LeuThr: 4.441 ± 0.348
4.256LeuVal: 4.256 ± 0.258
0.905LeuTrp: 0.905 ± 0.138
2.673LeuTyr: 2.673 ± 0.27
0.0LeuXaa: 0.0 ± 0.0
Met
2.426MetAla: 2.426 ± 0.224
0.267MetCys: 0.267 ± 0.079
1.501MetAsp: 1.501 ± 0.172
1.727MetGlu: 1.727 ± 0.232
1.46MetPhe: 1.46 ± 0.166
1.316MetGly: 1.316 ± 0.181
0.493MetHis: 0.493 ± 0.125
1.912MetIle: 1.912 ± 0.227
2.96MetLys: 2.96 ± 0.305
2.303MetLeu: 2.303 ± 0.169
0.966MetMet: 0.966 ± 0.141
1.686MetAsn: 1.686 ± 0.179
0.925MetPro: 0.925 ± 0.121
1.007MetGln: 1.007 ± 0.162
1.316MetArg: 1.316 ± 0.173
2.323MetSer: 2.323 ± 0.208
1.665MetThr: 1.665 ± 0.164
1.562MetVal: 1.562 ± 0.178
0.308MetTrp: 0.308 ± 0.084
1.028MetTyr: 1.028 ± 0.134
0.0MetXaa: 0.0 ± 0.0
Asn
3.783AsnAla: 3.783 ± 0.293
0.658AsnCys: 0.658 ± 0.102
3.31AsnAsp: 3.31 ± 0.245
3.803AsnGlu: 3.803 ± 0.257
2.056AsnPhe: 2.056 ± 0.188
4.585AsnGly: 4.585 ± 0.454
0.843AsnHis: 0.843 ± 0.133
3.495AsnIle: 3.495 ± 0.252
3.618AsnLys: 3.618 ± 0.298
3.968AsnLeu: 3.968 ± 0.356
1.295AsnMet: 1.295 ± 0.189
2.94AsnAsn: 2.94 ± 0.333
2.035AsnPro: 2.035 ± 0.2
1.871AsnGln: 1.871 ± 0.183
2.796AsnArg: 2.796 ± 0.223
3.351AsnSer: 3.351 ± 0.266
3.002AsnThr: 3.002 ± 0.268
3.577AsnVal: 3.577 ± 0.3
0.802AsnTrp: 0.802 ± 0.131
2.076AsnTyr: 2.076 ± 0.209
0.0AsnXaa: 0.0 ± 0.0
Pro
2.549ProAla: 2.549 ± 0.211
0.329ProCys: 0.329 ± 0.076
2.344ProAsp: 2.344 ± 0.198
2.94ProGlu: 2.94 ± 0.272
1.419ProPhe: 1.419 ± 0.145
2.59ProGly: 2.59 ± 0.265
0.637ProHis: 0.637 ± 0.121
2.261ProIle: 2.261 ± 0.217
2.2ProLys: 2.2 ± 0.232
2.179ProLeu: 2.179 ± 0.181
0.966ProMet: 0.966 ± 0.152
1.809ProAsn: 1.809 ± 0.206
0.987ProPro: 0.987 ± 0.161
0.884ProGln: 0.884 ± 0.15
1.357ProArg: 1.357 ± 0.173
2.179ProSer: 2.179 ± 0.181
2.405ProThr: 2.405 ± 0.241
2.549ProVal: 2.549 ± 0.19
0.72ProTrp: 0.72 ± 0.144
1.192ProTyr: 1.192 ± 0.178
0.0ProXaa: 0.0 ± 0.0
Gln
2.488GlnAla: 2.488 ± 0.21
0.164GlnCys: 0.164 ± 0.056
1.891GlnAsp: 1.891 ± 0.202
2.159GlnGlu: 2.159 ± 0.184
1.377GlnPhe: 1.377 ± 0.156
2.159GlnGly: 2.159 ± 0.22
0.678GlnHis: 0.678 ± 0.126
2.179GlnIle: 2.179 ± 0.201
2.323GlnLys: 2.323 ± 0.196
2.981GlnLeu: 2.981 ± 0.233
1.048GlnMet: 1.048 ± 0.148
1.562GlnAsn: 1.562 ± 0.189
0.925GlnPro: 0.925 ± 0.157
1.069GlnGln: 1.069 ± 0.173
1.747GlnArg: 1.747 ± 0.174
1.891GlnSer: 1.891 ± 0.185
1.974GlnThr: 1.974 ± 0.252
2.631GlnVal: 2.631 ± 0.26
0.596GlnTrp: 0.596 ± 0.116
1.562GlnTyr: 1.562 ± 0.189
0.0GlnXaa: 0.0 ± 0.0
Arg
3.145ArgAla: 3.145 ± 0.261
0.555ArgCys: 0.555 ± 0.125
2.734ArgAsp: 2.734 ± 0.247
3.515ArgGlu: 3.515 ± 0.258
1.891ArgPhe: 1.891 ± 0.199
3.125ArgGly: 3.125 ± 0.243
0.843ArgHis: 0.843 ± 0.12
3.248ArgIle: 3.248 ± 0.255
3.33ArgLys: 3.33 ± 0.254
3.536ArgLeu: 3.536 ± 0.306
1.09ArgMet: 1.09 ± 0.136
2.2ArgAsn: 2.2 ± 0.21
1.172ArgPro: 1.172 ± 0.146
1.604ArgGln: 1.604 ± 0.164
2.549ArgArg: 2.549 ± 0.259
2.611ArgSer: 2.611 ± 0.242
2.673ArgThr: 2.673 ± 0.221
3.022ArgVal: 3.022 ± 0.237
1.048ArgTrp: 1.048 ± 0.153
1.994ArgTyr: 1.994 ± 0.202
0.0ArgXaa: 0.0 ± 0.0
Ser
4.667SerAla: 4.667 ± 0.366
0.761SerCys: 0.761 ± 0.113
3.988SerAsp: 3.988 ± 0.273
3.927SerGlu: 3.927 ± 0.299
2.611SerPhe: 2.611 ± 0.219
4.399SerGly: 4.399 ± 0.295
1.172SerHis: 1.172 ± 0.166
3.824SerIle: 3.824 ± 0.317
4.852SerLys: 4.852 ± 0.344
4.543SerLeu: 4.543 ± 0.292
1.419SerMet: 1.419 ± 0.151
3.372SerAsn: 3.372 ± 0.31
2.118SerPro: 2.118 ± 0.192
1.809SerGln: 1.809 ± 0.192
2.796SerArg: 2.796 ± 0.23
4.05SerSer: 4.05 ± 0.315
3.886SerThr: 3.886 ± 0.295
4.153SerVal: 4.153 ± 0.279
0.802SerTrp: 0.802 ± 0.133
2.508SerTyr: 2.508 ± 0.218
0.0SerXaa: 0.0 ± 0.0
Thr
4.523ThrAla: 4.523 ± 0.384
0.493ThrCys: 0.493 ± 0.113
2.96ThrAsp: 2.96 ± 0.313
3.577ThrGlu: 3.577 ± 0.278
2.816ThrPhe: 2.816 ± 0.255
4.173ThrGly: 4.173 ± 0.457
0.946ThrHis: 0.946 ± 0.135
4.399ThrIle: 4.399 ± 0.289
3.557ThrLys: 3.557 ± 0.223
4.42ThrLeu: 4.42 ± 0.386
1.336ThrMet: 1.336 ± 0.162
2.816ThrAsn: 2.816 ± 0.236
2.878ThrPro: 2.878 ± 0.304
1.871ThrGln: 1.871 ± 0.254
2.858ThrArg: 2.858 ± 0.209
3.577ThrSer: 3.577 ± 0.377
3.844ThrThr: 3.844 ± 0.321
4.893ThrVal: 4.893 ± 0.353
0.925ThrTrp: 0.925 ± 0.142
2.159ThrTyr: 2.159 ± 0.215
0.0ThrXaa: 0.0 ± 0.0
Val
4.358ValAla: 4.358 ± 0.342
0.822ValCys: 0.822 ± 0.121
4.79ValAsp: 4.79 ± 0.324
4.934ValGlu: 4.934 ± 0.307
3.248ValPhe: 3.248 ± 0.246
3.639ValGly: 3.639 ± 0.299
1.069ValHis: 1.069 ± 0.145
4.543ValIle: 4.543 ± 0.266
6.065ValLys: 6.065 ± 0.346
4.112ValLeu: 4.112 ± 0.264
2.035ValMet: 2.035 ± 0.183
3.886ValAsn: 3.886 ± 0.266
2.467ValPro: 2.467 ± 0.235
2.858ValGln: 2.858 ± 0.219
3.063ValArg: 3.063 ± 0.259
3.701ValSer: 3.701 ± 0.314
3.598ValThr: 3.598 ± 0.301
4.955ValVal: 4.955 ± 0.336
1.09ValTrp: 1.09 ± 0.141
2.796ValTyr: 2.796 ± 0.248
0.0ValXaa: 0.0 ± 0.0
Trp
0.637TrpAla: 0.637 ± 0.113
0.164TrpCys: 0.164 ± 0.052
0.987TrpAsp: 0.987 ± 0.136
0.925TrpGlu: 0.925 ± 0.138
0.863TrpPhe: 0.863 ± 0.135
0.637TrpGly: 0.637 ± 0.119
0.329TrpHis: 0.329 ± 0.097
0.946TrpIle: 0.946 ± 0.14
1.604TrpLys: 1.604 ± 0.209
1.172TrpLeu: 1.172 ± 0.152
0.678TrpMet: 0.678 ± 0.103
1.007TrpAsn: 1.007 ± 0.157
0.37TrpPro: 0.37 ± 0.078
0.514TrpGln: 0.514 ± 0.094
0.596TrpArg: 0.596 ± 0.1
0.884TrpSer: 0.884 ± 0.132
1.09TrpThr: 1.09 ± 0.145
1.028TrpVal: 1.028 ± 0.141
0.349TrpTrp: 0.349 ± 0.087
0.843TrpTyr: 0.843 ± 0.148
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.145TyrAla: 3.145 ± 0.26
0.473TyrCys: 0.473 ± 0.092
3.084TyrAsp: 3.084 ± 0.246
2.488TyrGlu: 2.488 ± 0.229
1.665TyrPhe: 1.665 ± 0.164
2.446TyrGly: 2.446 ± 0.217
0.781TyrHis: 0.781 ± 0.116
2.816TyrIle: 2.816 ± 0.28
2.775TyrLys: 2.775 ± 0.233
2.426TyrLeu: 2.426 ± 0.21
0.925TyrMet: 0.925 ± 0.126
2.652TyrAsn: 2.652 ± 0.238
1.747TyrPro: 1.747 ± 0.15
1.46TyrGln: 1.46 ± 0.178
2.056TyrArg: 2.056 ± 0.244
2.529TyrSer: 2.529 ± 0.25
1.789TyrThr: 1.789 ± 0.153
3.022TyrVal: 3.022 ± 0.314
0.658TyrTrp: 0.658 ± 0.107
1.645TyrTyr: 1.645 ± 0.2
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.021XaaAla: 0.021 ± 0.018
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.493XaaXaa: 0.493 ± 0.443
Statistics based on 252 proteins (48643 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski