Porcine mastadenovirus B

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Rowavirales; Adenoviridae; Mastadenovirus

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7H0S529|A0A7H0S529_9ADEN E1B protein small T-antigen OS=Porcine mastadenovirus B OX=130370 PE=3 SV=1
MM1 pKa = 7.6AGKK4 pKa = 8.31ITLTLSRR11 pKa = 11.84SLLEE15 pKa = 4.14AARR18 pKa = 11.84LAMDD22 pKa = 4.97PSTRR26 pKa = 11.84PVSCLEE32 pKa = 3.8LLGPQGPSLHH42 pKa = 7.17DD43 pKa = 4.02LFDD46 pKa = 4.21IEE48 pKa = 4.9PEE50 pKa = 4.39PGSTLPAPVEE60 pKa = 3.85LAVPSTLYY68 pKa = 10.98ASAAAYY74 pKa = 10.19FGMEE78 pKa = 4.16PSSAVDD84 pKa = 3.03ITPPCSPSPRR94 pKa = 11.84DD95 pKa = 3.6PEE97 pKa = 4.73IVDD100 pKa = 3.77LTCDD104 pKa = 4.01EE105 pKa = 4.32ILQPAEE111 pKa = 4.34EE112 pKa = 4.92EE113 pKa = 4.34PCEE116 pKa = 4.12PAPAPNPGGDD126 pKa = 3.87DD127 pKa = 4.12GILPVFQLDD136 pKa = 4.03CPPVPGHH143 pKa = 6.28NCASCNYY150 pKa = 9.38HH151 pKa = 7.23RR152 pKa = 11.84DD153 pKa = 3.48RR154 pKa = 11.84LGDD157 pKa = 3.85PNVTCALCYY166 pKa = 10.14FRR168 pKa = 11.84LSHH171 pKa = 6.88HH172 pKa = 6.5MIFGKK177 pKa = 10.48CCMSVGTT184 pKa = 4.64

Molecular weight: 19.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7H0S578|A0A7H0S578_9ADEN Hexon protein OS=Porcine mastadenovirus B OX=130370 GN=L3 PE=2 SV=1
MM1 pKa = 7.64GPRR4 pKa = 11.84PKK6 pKa = 10.03LVYY9 pKa = 10.31RR10 pKa = 11.84IRR12 pKa = 11.84VPVGLKK18 pKa = 10.1GRR20 pKa = 11.84GVNRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84LRR32 pKa = 11.84GGFLPALIPIIAAAIGAIPGIASVAVQASRR62 pKa = 11.84KK63 pKa = 7.51NN64 pKa = 3.39

Molecular weight: 6.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

31

3

34

10464

53

1145

307.8

34.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.703 ± 0.264

2.036 ± 0.322

5.122 ± 0.227

5.552 ± 0.36

3.909 ± 0.197

6.145 ± 0.313

2.705 ± 0.201

3.813 ± 0.245

3.555 ± 0.382

9.738 ± 0.382

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.446 ± 0.197

3.651 ± 0.392

7.511 ± 0.493

4.922 ± 0.321

6.632 ± 0.497

7.454 ± 0.309

6.078 ± 0.324

6.422 ± 0.349

1.29 ± 0.15

3.316 ± 0.306
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski