Invertebrate iridescent virus 3 (IIV-3) (Mosquito iridescent virus)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Iridoviridae; Betairidovirinae; Chloriridovirus

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 126 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q197F9|VF395_IIV3 Uncharacterized protein 001R OS=Invertebrate iridescent virus 3 OX=345201 GN=IIV3-001R PE=3 SV=1
MM1 pKa = 7.83ASNTVSAQGGSNRR14 pKa = 11.84PVRR17 pKa = 11.84DD18 pKa = 3.99FSNIQDD24 pKa = 3.47VAQFLLFDD32 pKa = 5.76PIWNEE37 pKa = 3.76QPGSIVPWKK46 pKa = 9.76MNRR49 pKa = 11.84EE50 pKa = 3.52QALAEE55 pKa = 4.57RR56 pKa = 11.84YY57 pKa = 9.34PEE59 pKa = 4.31LQTSEE64 pKa = 4.24PSEE67 pKa = 4.8DD68 pKa = 3.29YY69 pKa = 10.95SGPVEE74 pKa = 4.3SLEE77 pKa = 4.59LLPLEE82 pKa = 4.42IKK84 pKa = 10.52LDD86 pKa = 3.31IMQYY90 pKa = 10.86LSWEE94 pKa = 4.03QISWCKK100 pKa = 10.51HH101 pKa = 3.79PWLWTRR107 pKa = 11.84WYY109 pKa = 9.35KK110 pKa = 11.4DD111 pKa = 2.97NVVRR115 pKa = 11.84VSAITFEE122 pKa = 4.31DD123 pKa = 4.23FQRR126 pKa = 11.84EE127 pKa = 3.88YY128 pKa = 11.45AFPEE132 pKa = 4.63KK133 pKa = 10.04IQEE136 pKa = 3.74IHH138 pKa = 5.8FTDD141 pKa = 3.56TRR143 pKa = 11.84AEE145 pKa = 4.12EE146 pKa = 3.83IKK148 pKa = 10.69AILEE152 pKa = 4.05TTPNVTRR159 pKa = 11.84LVIRR163 pKa = 11.84RR164 pKa = 11.84IDD166 pKa = 3.48DD167 pKa = 3.38MNYY170 pKa = 8.06NTHH173 pKa = 6.75GDD175 pKa = 3.61LGLDD179 pKa = 3.25DD180 pKa = 6.25LEE182 pKa = 4.78FLTHH186 pKa = 7.12LMVEE190 pKa = 4.59DD191 pKa = 3.58ACGFTDD197 pKa = 4.98FWAPSLTHH205 pKa = 6.21LTIKK209 pKa = 10.42NLDD212 pKa = 3.26MHH214 pKa = 6.54PRR216 pKa = 11.84WFGPVMDD223 pKa = 6.2GIKK226 pKa = 10.61SMQSTLKK233 pKa = 10.13YY234 pKa = 10.38LYY236 pKa = 9.88IFEE239 pKa = 4.7TYY241 pKa = 10.04GVNKK245 pKa = 9.92PFVQWCTDD253 pKa = 3.54NIEE256 pKa = 4.33TFYY259 pKa = 10.49CTNSYY264 pKa = 10.33RR265 pKa = 11.84YY266 pKa = 9.28EE267 pKa = 4.06NVPRR271 pKa = 11.84PIYY274 pKa = 10.44VWVLFQEE281 pKa = 4.93DD282 pKa = 3.58EE283 pKa = 3.92WHH285 pKa = 6.76GYY287 pKa = 8.4RR288 pKa = 11.84VEE290 pKa = 4.19DD291 pKa = 3.39NKK293 pKa = 10.19FHH295 pKa = 6.72RR296 pKa = 11.84RR297 pKa = 11.84YY298 pKa = 8.61MYY300 pKa = 9.69STILHH305 pKa = 6.22KK306 pKa = 10.47RR307 pKa = 11.84DD308 pKa = 3.52TDD310 pKa = 3.51WVEE313 pKa = 3.87NNPLKK318 pKa = 10.3TPAQVEE324 pKa = 4.56MYY326 pKa = 10.32KK327 pKa = 10.55FLLRR331 pKa = 11.84ISQLNRR337 pKa = 11.84DD338 pKa = 3.6GTGYY342 pKa = 10.78EE343 pKa = 4.08SDD345 pKa = 3.54SDD347 pKa = 4.1PEE349 pKa = 4.16NEE351 pKa = 4.48HH352 pKa = 6.87FDD354 pKa = 4.21DD355 pKa = 4.66EE356 pKa = 4.89SFSSGEE362 pKa = 3.99EE363 pKa = 4.03DD364 pKa = 5.31SSDD367 pKa = 3.6EE368 pKa = 5.6DD369 pKa = 4.11DD370 pKa = 4.44PTWAPDD376 pKa = 3.51SDD378 pKa = 3.94DD379 pKa = 4.79SDD381 pKa = 3.73WEE383 pKa = 4.49TEE385 pKa = 4.2TEE387 pKa = 4.19EE388 pKa = 4.45EE389 pKa = 4.21PSVAARR395 pKa = 11.84ILEE398 pKa = 4.25KK399 pKa = 10.97GKK401 pKa = 8.91LTITNLMKK409 pKa = 10.84SLGFKK414 pKa = 10.09PKK416 pKa = 9.46PKK418 pKa = 9.99KK419 pKa = 9.46IQSIDD424 pKa = 3.33RR425 pKa = 11.84YY426 pKa = 10.31FCSLDD431 pKa = 3.4SNYY434 pKa = 10.45NSEE437 pKa = 5.38DD438 pKa = 3.25EE439 pKa = 4.32DD440 pKa = 4.12FEE442 pKa = 4.73YY443 pKa = 10.99DD444 pKa = 4.65SDD446 pKa = 5.66SEE448 pKa = 5.57DD449 pKa = 4.43DD450 pKa = 5.17DD451 pKa = 5.62SDD453 pKa = 5.89SEE455 pKa = 4.5DD456 pKa = 3.46DD457 pKa = 3.8CC458 pKa = 7.55

Molecular weight: 53.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q196W2|VF439_IIV3 Probable kinase 098L OS=Invertebrate iridescent virus 3 OX=345201 GN=IIV3-098L PE=3 SV=1
MM1 pKa = 7.53NDD3 pKa = 2.88VHH5 pKa = 7.45DD6 pKa = 4.38CLVWVNDD13 pKa = 4.43PYY15 pKa = 11.23FHH17 pKa = 7.6PISKK21 pKa = 10.41RR22 pKa = 11.84PIKK25 pKa = 10.7YY26 pKa = 9.82KK27 pKa = 10.33GPTWRR32 pKa = 11.84HH33 pKa = 4.3YY34 pKa = 10.83DD35 pKa = 3.65KK36 pKa = 11.33KK37 pKa = 10.89CDD39 pKa = 3.88LLGITGPKK47 pKa = 8.0ATKK50 pKa = 9.98SPSRR54 pKa = 11.84RR55 pKa = 11.84TTRR58 pKa = 11.84SPSPSRR64 pKa = 11.84RR65 pKa = 11.84TTRR68 pKa = 11.84SSPSRR73 pKa = 11.84RR74 pKa = 11.84TTRR77 pKa = 11.84SSPSRR82 pKa = 11.84RR83 pKa = 11.84TTRR86 pKa = 11.84SPSPSGRR93 pKa = 11.84RR94 pKa = 11.84KK95 pKa = 9.23QGGPAVYY102 pKa = 9.99CGNNALDD109 pKa = 4.11EE110 pKa = 4.48GLLDD114 pKa = 3.8GSKK117 pKa = 10.76VVGTRR122 pKa = 11.84YY123 pKa = 9.36QCLQKK128 pKa = 10.96GVAVGLNNPVLHH140 pKa = 6.81HH141 pKa = 6.65SPNYY145 pKa = 9.76QPIVDD150 pKa = 3.7AKK152 pKa = 9.33IYY154 pKa = 10.57CGTGSKK160 pKa = 10.61LPASKK165 pKa = 10.53LRR167 pKa = 11.84FGTPTEE173 pKa = 4.23CMGKK177 pKa = 10.28GYY179 pKa = 10.54QIGQNKK185 pKa = 9.3RR186 pKa = 11.84FQQSGLQQGPYY197 pKa = 9.18IWEE200 pKa = 3.6EE201 pKa = 4.0DD202 pKa = 3.07GWYY205 pKa = 10.41KK206 pKa = 10.62IIVPKK211 pKa = 10.75NN212 pKa = 3.12

Molecular weight: 23.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

126

0

126

43365

60

1377

344.2

39.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.145 ± 0.192

1.783 ± 0.102

5.8 ± 0.119

5.654 ± 0.227

4.423 ± 0.152

4.983 ± 0.246

2.181 ± 0.131

5.938 ± 0.231

7.089 ± 0.238

8.982 ± 0.208

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.122 ± 0.086

5.313 ± 0.128

5.447 ± 0.245

4.381 ± 0.123

4.822 ± 0.181

7.059 ± 0.242

6.768 ± 0.205

6.874 ± 0.173

1.351 ± 0.088

3.886 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski