Blastopirellula marina DSM 3645
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6010 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A3ZPB0|A3ZPB0_9BACT Fe(III) dicitrate ABC transporter permease OS=Blastopirellula marina DSM 3645 OX=314230 GN=DSM3645_28442 PE=3 SV=1
MM1 pKa = 7.51 KK2 pKa = 10.05 LCRR5 pKa = 11.84 WICLFITMWIAGPVYY20 pKa = 10.78 AEE22 pKa = 4.29 TLSIDD27 pKa = 3.21 ATADD31 pKa = 3.3 GSSRR35 pKa = 11.84 WSEE38 pKa = 3.99 YY39 pKa = 10.62 FSDD42 pKa = 5.0 AFVQLDD48 pKa = 4.12 HH49 pKa = 6.95 QPGSYY54 pKa = 10.36 LISEE58 pKa = 4.47 YY59 pKa = 10.46 EE60 pKa = 3.69 ADD62 pKa = 3.54 GSYY65 pKa = 11.17 VPVGDD70 pKa = 4.24 GSQIAFLNDD79 pKa = 2.77 GDD81 pKa = 4.35 FNSFFDD87 pKa = 4.71 IEE89 pKa = 4.24 FTAPAGRR96 pKa = 11.84 SGTVAIDD103 pKa = 3.36 AFTADD108 pKa = 4.13 FDD110 pKa = 5.79 DD111 pKa = 7.02 FIADD115 pKa = 4.09 DD116 pKa = 4.18 DD117 pKa = 5.78 AIFNTGYY124 pKa = 9.19 ATTINSFTGTATFVGGVISQIDD146 pKa = 4.07 LLADD150 pKa = 3.26 IMLTYY155 pKa = 10.28 DD156 pKa = 3.1 ASGFGLGMLDD166 pKa = 3.46 YY167 pKa = 10.82 AGTFAITGAEE177 pKa = 3.93 FALFADD183 pKa = 5.14 GSYY186 pKa = 8.89 EE187 pKa = 4.24 TGFTPARR194 pKa = 11.84 YY195 pKa = 8.69 VWDD198 pKa = 3.54 VTGTLDD204 pKa = 4.87 LPEE207 pKa = 5.34 PPTATPEE214 pKa = 4.36 PGSLLLAVVGGVGLLTFRR232 pKa = 11.84 RR233 pKa = 11.84 RR234 pKa = 11.84 RR235 pKa = 11.84 KK236 pKa = 9.78 RR237 pKa = 11.84 VTAEE241 pKa = 3.48
Molecular weight: 25.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.757
IPC_protein 3.77
Toseland 3.541
ProMoST 3.935
Dawson 3.783
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.592
Grimsley 3.452
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.164
Thurlkill 3.605
EMBOSS 3.745
Sillero 3.897
Patrickios 1.888
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.826
Protein with the highest isoelectric point:
>tr|A3ZVR0|A3ZVR0_9BACT Uncharacterized protein OS=Blastopirellula marina DSM 3645 OX=314230 GN=DSM3645_02983 PE=4 SV=1
MM1 pKa = 7.31 TPILKK6 pKa = 10.33 GALSLGRR13 pKa = 11.84 TFGRR17 pKa = 11.84 RR18 pKa = 11.84 LISVSSGWQRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 FTKK33 pKa = 10.41 KK34 pKa = 10.06 SRR36 pKa = 3.27
Molecular weight: 4.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.471
IPC2_protein 11.023
IPC_protein 12.618
Toseland 12.778
ProMoST 13.29
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.457
Grimsley 12.822
Solomon 13.276
Lehninger 13.188
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.193
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6010
0
6010
1930168
20
9105
321.2
35.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.331 ± 0.039
1.112 ± 0.014
5.959 ± 0.031
6.294 ± 0.03
3.762 ± 0.02
7.518 ± 0.035
2.103 ± 0.017
5.305 ± 0.023
3.843 ± 0.03
9.884 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.294 ± 0.017
3.228 ± 0.024
5.158 ± 0.028
4.322 ± 0.024
6.313 ± 0.042
6.237 ± 0.031
5.39 ± 0.036
6.866 ± 0.029
1.471 ± 0.014
2.612 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here