Vibrio phage pYD38-A
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R9TRY7|R9TRY7_9CAUD Uncharacterized protein OS=Vibrio phage pYD38-A OX=754051 GN=VPRG_00042 PE=4 SV=1
MM1 pKa = 8.2 IIEE4 pKa = 4.3 EE5 pKa = 4.49 TEE7 pKa = 3.79 NAYY10 pKa = 10.86 DD11 pKa = 3.44 MHH13 pKa = 5.74 VTEE16 pKa = 5.67 QEE18 pKa = 4.14 LDD20 pKa = 3.51 DD21 pKa = 4.11 GTPVVEE27 pKa = 4.88 LYY29 pKa = 10.12 QVNGSGVEE37 pKa = 4.02 SFIGIDD43 pKa = 3.24 KK44 pKa = 10.25 HH45 pKa = 6.49 QAAQLII51 pKa = 4.22
Molecular weight: 5.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.8
IPC2_protein 3.948
IPC_protein 3.77
Toseland 3.617
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.668
Rodwell 3.63
Grimsley 3.541
Solomon 3.719
Lehninger 3.668
Nozaki 3.897
DTASelect 3.999
Thurlkill 3.681
EMBOSS 3.681
Sillero 3.897
Patrickios 1.863
IPC_peptide 3.719
IPC2_peptide 3.872
IPC2.peptide.svr19 3.81
Protein with the highest isoelectric point:
>tr|R9TRE3|R9TRE3_9CAUD Uncharacterized protein OS=Vibrio phage pYD38-A OX=754051 GN=VPRG_00025 PE=4 SV=1
MM1 pKa = 7.41 SKK3 pKa = 10.93 ASTLHH8 pKa = 5.51 EE9 pKa = 5.45 LIMADD14 pKa = 2.86 IRR16 pKa = 11.84 EE17 pKa = 4.41 SNARR21 pKa = 11.84 ADD23 pKa = 3.64 WQRR26 pKa = 11.84 NQPDD30 pKa = 3.54 RR31 pKa = 11.84 RR32 pKa = 11.84 TLKK35 pKa = 10.54 QKK37 pKa = 9.69 LHH39 pKa = 6.18 PKK41 pKa = 9.53 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 PNRR47 pKa = 11.84 KK48 pKa = 7.81 RR49 pKa = 11.84 DD50 pKa = 3.4 YY51 pKa = 10.89 RR52 pKa = 11.84 RR53 pKa = 11.84 DD54 pKa = 3.23 RR55 pKa = 11.84 VLRR58 pKa = 11.84 KK59 pKa = 10.12 LCDD62 pKa = 3.17 IQMKK66 pKa = 9.79 FMIKK70 pKa = 10.14 EE71 pKa = 4.14 AMNEE75 pKa = 4.28 TNDD78 pKa = 3.46 EE79 pKa = 4.18
Molecular weight: 9.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.575
IPC_protein 10.248
Toseland 10.921
ProMoST 10.628
Dawson 10.965
Bjellqvist 10.672
Wikipedia 11.184
Rodwell 11.169
Grimsley 10.994
Solomon 11.14
Lehninger 11.111
Nozaki 10.891
DTASelect 10.657
Thurlkill 10.891
EMBOSS 11.33
Sillero 10.906
Patrickios 10.921
IPC_peptide 11.155
IPC2_peptide 9.516
IPC2.peptide.svr19 8.847
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
77
0
77
14315
51
1097
185.9
20.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.046 ± 0.788
1.271 ± 0.138
6.161 ± 0.296
6.518 ± 0.278
3.185 ± 0.202
7.118 ± 0.224
1.746 ± 0.201
6.196 ± 0.171
6.35 ± 0.361
7.328 ± 0.227
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.955 ± 0.17
4.897 ± 0.297
3.507 ± 0.241
4.024 ± 0.317
5.176 ± 0.273
6.574 ± 0.308
5.868 ± 0.447
6.846 ± 0.244
1.614 ± 0.138
3.619 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here