Shayang ascaridia galli virus 2
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KN28|A0A1L3KN28_9VIRU Putative nucleoprotein OS=Shayang ascaridia galli virus 2 OX=1923460 PE=4 SV=1
MM1 pKa = 8.14 DD2 pKa = 5.69 SNDD5 pKa = 3.5 TLLDD9 pKa = 3.95 GNEE12 pKa = 3.91 LQEE15 pKa = 4.69 AEE17 pKa = 4.13 HH18 pKa = 6.65 HH19 pKa = 6.54 KK20 pKa = 11.17 NSLLALAQDD29 pKa = 3.87 TNFVSKK35 pKa = 9.64 YY36 pKa = 8.33 TLTSGDD42 pKa = 3.62 FADD45 pKa = 4.99 TEE47 pKa = 4.31 DD48 pKa = 4.98 PYY50 pKa = 11.88 DD51 pKa = 3.65 DD52 pKa = 4.37 TRR54 pKa = 11.84 FQLPQTPTSEE64 pKa = 4.35 STTTAASYY72 pKa = 9.93 PPRR75 pKa = 11.84 TYY77 pKa = 9.98 ATSSSDD83 pKa = 3.08 HH84 pKa = 6.92 SDD86 pKa = 3.67 DD87 pKa = 5.54 LRR89 pKa = 11.84 HH90 pKa = 6.53 KK91 pKa = 10.11 YY92 pKa = 10.04 INEE95 pKa = 3.61 AAAALHH101 pKa = 6.27 KK102 pKa = 10.76 AGIPIDD108 pKa = 4.41 SINQFLYY115 pKa = 9.81 THH117 pKa = 7.47 DD118 pKa = 3.77 KK119 pKa = 10.67 NKK121 pKa = 10.5 AGFWEE126 pKa = 5.01 GVVQCANLFLTKK138 pKa = 10.32 SSSQALDD145 pKa = 4.75 AIPLLHH151 pKa = 6.84 SKK153 pKa = 9.93 IDD155 pKa = 3.72 SLQKK159 pKa = 10.82 QMANLCEE166 pKa = 4.24 LLTTNDD172 pKa = 3.3 KK173 pKa = 10.06 LTVQNHH179 pKa = 5.23 NKK181 pKa = 10.17 EE182 pKa = 4.59 SVQSQQHH189 pKa = 5.91 SGIQPEE195 pKa = 4.53 PKK197 pKa = 8.74 PQSVTPLEE205 pKa = 4.11 IKK207 pKa = 10.81 FNDD210 pKa = 3.62 TTPLYY215 pKa = 10.87 ARR217 pKa = 11.84 EE218 pKa = 4.13 AVTNLPIASACKK230 pKa = 9.93 QYY232 pKa = 11.13 LADD235 pKa = 3.66 CMARR239 pKa = 11.84 EE240 pKa = 4.41 FIPWSFLHH248 pKa = 7.15 AIHH251 pKa = 6.73 NATSPNMKK259 pKa = 8.42 RR260 pKa = 11.84 TSYY263 pKa = 11.44 LEE265 pKa = 3.82 MIEE268 pKa = 3.96 YY269 pKa = 8.53 MRR271 pKa = 11.84 SHH273 pKa = 6.46 GMPPDD278 pKa = 3.44 VYY280 pKa = 11.48 DD281 pKa = 5.04 FFIKK285 pKa = 10.08 PWNN288 pKa = 3.54
Molecular weight: 32.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.105
IPC2_protein 5.118
IPC_protein 5.105
Toseland 5.245
ProMoST 5.334
Dawson 5.207
Bjellqvist 5.296
Wikipedia 5.143
Rodwell 5.13
Grimsley 5.245
Solomon 5.207
Lehninger 5.181
Nozaki 5.385
DTASelect 5.575
Thurlkill 5.308
EMBOSS 5.296
Sillero 5.448
Patrickios 3.706
IPC_peptide 5.219
IPC2_peptide 5.461
IPC2.peptide.svr19 5.354
Protein with the highest isoelectric point:
>tr|A0A1L3KN19|A0A1L3KN19_9VIRU Uncharacterized protein OS=Shayang ascaridia galli virus 2 OX=1923460 PE=4 SV=1
MM1 pKa = 6.33 TTYY4 pKa = 11.34 LVMNMSGVIDD14 pKa = 3.85 VSVAPPADD22 pKa = 3.47 KK23 pKa = 11.19 QEE25 pKa = 4.39 TVDD28 pKa = 3.79 IACQMVMAAITPPIPTEE45 pKa = 3.76 LRR47 pKa = 11.84 AAAARR52 pKa = 11.84 ILCNKK57 pKa = 9.77 LDD59 pKa = 3.76 EE60 pKa = 4.69 CRR62 pKa = 11.84 PLQSTVITSNGKK74 pKa = 9.54 VPGLRR79 pKa = 11.84 YY80 pKa = 8.95 PVLLDD85 pKa = 3.2 GKK87 pKa = 10.32 FAVKK91 pKa = 10.43 LKK93 pKa = 10.43 ADD95 pKa = 3.74 RR96 pKa = 11.84 PTRR99 pKa = 11.84 DD100 pKa = 3.75 FVSLHH105 pKa = 5.86 CPQRR109 pKa = 11.84 LDD111 pKa = 5.17 NEE113 pKa = 4.52 DD114 pKa = 4.37 DD115 pKa = 3.88 NNNSLFIHH123 pKa = 6.22 LTLSAEE129 pKa = 4.02 LGIINGVTANQLTITGRR146 pKa = 11.84 HH147 pKa = 4.54 IVPYY151 pKa = 10.05 LGNDD155 pKa = 3.12 EE156 pKa = 4.22 RR157 pKa = 11.84 FEE159 pKa = 5.23 AIAAASIKK167 pKa = 10.33 KK168 pKa = 10.46 KK169 pKa = 10.57 SDD171 pKa = 3.36 TPKK174 pKa = 10.17 SPIRR178 pKa = 11.84 RR179 pKa = 11.84 FAALFKK185 pKa = 10.74 KK186 pKa = 10.39 KK187 pKa = 9.85 NHH189 pKa = 5.64 NN190 pKa = 3.5
Molecular weight: 20.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.374
IPC2_protein 8.229
IPC_protein 8.2
Toseland 8.712
ProMoST 8.697
Dawson 9.063
Bjellqvist 9.092
Wikipedia 9.077
Rodwell 9.224
Grimsley 8.595
Solomon 9.253
Lehninger 9.253
Nozaki 9.385
DTASelect 8.916
Thurlkill 9.063
EMBOSS 9.268
Sillero 9.282
Patrickios 5.156
IPC_peptide 9.253
IPC2_peptide 7.878
IPC2.peptide.svr19 7.749
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4061
190
2222
676.8
75.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.535 ± 0.636
2.241 ± 0.38
5.27 ± 0.417
5.319 ± 0.307
4.186 ± 0.376
4.432 ± 0.294
2.339 ± 0.31
5.639 ± 0.509
5.097 ± 0.281
10.81 ± 0.965
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.413 ± 0.192
4.26 ± 0.582
5.023 ± 0.345
3.644 ± 0.468
5.294 ± 0.542
8.422 ± 0.39
6.722 ± 0.746
6.846 ± 0.578
1.551 ± 0.288
2.955 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here