Ageratum leaf curl betasatellite
Average proteome isoelectric point is 5.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M4UQM4|M4UQM4_9VIRU C1 OS=Ageratum leaf curl betasatellite OX=1175505 GN=C1 PE=4 SV=1
MM1 pKa = 7.67 SINHH5 pKa = 7.31 DD6 pKa = 3.37 ICPLLRR12 pKa = 11.84 RR13 pKa = 11.84 INSRR17 pKa = 11.84 TKK19 pKa = 9.69 SQLEE23 pKa = 3.83 IMTIKK28 pKa = 10.76 YY29 pKa = 10.57 NNTKK33 pKa = 10.21 GMEE36 pKa = 3.8 FTIDD40 pKa = 3.42 VKK42 pKa = 11.09 LKK44 pKa = 8.97 EE45 pKa = 4.32 GNSIIVQIEE54 pKa = 4.21 LCSTRR59 pKa = 11.84 SPTLAKK65 pKa = 10.0 KK66 pKa = 10.36 IFMIPYY72 pKa = 8.38 GHH74 pKa = 7.45 DD75 pKa = 3.85 GIIPPFDD82 pKa = 3.94 FNNLEE87 pKa = 3.87 EE88 pKa = 4.88 GIRR91 pKa = 11.84 NILKK95 pKa = 10.25 FMYY98 pKa = 9.56 KK99 pKa = 9.98 DD100 pKa = 3.2 SHH102 pKa = 8.08 IGDD105 pKa = 4.26 FRR107 pKa = 11.84 QEE109 pKa = 3.99 DD110 pKa = 3.94 MVEE113 pKa = 4.2 IIDD116 pKa = 3.77 MLMMQEE122 pKa = 4.56 APVIDD127 pKa = 3.83 IRR129 pKa = 11.84 IADD132 pKa = 4.84 DD133 pKa = 4.38 YY134 pKa = 11.37 DD135 pKa = 3.23 ICTNATVV142 pKa = 3.21
Molecular weight: 16.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.058
IPC2_protein 5.13
IPC_protein 5.016
Toseland 4.952
ProMoST 5.169
Dawson 5.029
Bjellqvist 5.156
Wikipedia 4.914
Rodwell 4.927
Grimsley 4.876
Solomon 5.016
Lehninger 4.978
Nozaki 5.143
DTASelect 5.334
Thurlkill 4.965
EMBOSS 4.965
Sillero 5.207
Patrickios 4.126
IPC_peptide 5.029
IPC2_peptide 5.207
IPC2.peptide.svr19 5.198
Protein with the highest isoelectric point:
>tr|M4UQM4|M4UQM4_9VIRU C1 OS=Ageratum leaf curl betasatellite OX=1175505 GN=C1 PE=4 SV=1
MM1 pKa = 7.67 SINHH5 pKa = 7.31 DD6 pKa = 3.37 ICPLLRR12 pKa = 11.84 RR13 pKa = 11.84 INSRR17 pKa = 11.84 TKK19 pKa = 9.69 SQLEE23 pKa = 3.83 IMTIKK28 pKa = 10.76 YY29 pKa = 10.57 NNTKK33 pKa = 10.21 GMEE36 pKa = 3.8 FTIDD40 pKa = 3.42 VKK42 pKa = 11.09 LKK44 pKa = 8.97 EE45 pKa = 4.32 GNSIIVQIEE54 pKa = 4.21 LCSTRR59 pKa = 11.84 SPTLAKK65 pKa = 10.0 KK66 pKa = 10.36 IFMIPYY72 pKa = 8.38 GHH74 pKa = 7.45 DD75 pKa = 3.85 GIIPPFDD82 pKa = 3.94 FNNLEE87 pKa = 3.87 EE88 pKa = 4.88 GIRR91 pKa = 11.84 NILKK95 pKa = 10.25 FMYY98 pKa = 9.56 KK99 pKa = 9.98 DD100 pKa = 3.2 SHH102 pKa = 8.08 IGDD105 pKa = 4.26 FRR107 pKa = 11.84 QEE109 pKa = 3.99 DD110 pKa = 3.94 MVEE113 pKa = 4.2 IIDD116 pKa = 3.77 MLMMQEE122 pKa = 4.56 APVIDD127 pKa = 3.83 IRR129 pKa = 11.84 IADD132 pKa = 4.84 DD133 pKa = 4.38 YY134 pKa = 11.37 DD135 pKa = 3.23 ICTNATVV142 pKa = 3.21
Molecular weight: 16.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.058
IPC2_protein 5.13
IPC_protein 5.016
Toseland 4.952
ProMoST 5.169
Dawson 5.029
Bjellqvist 5.156
Wikipedia 4.914
Rodwell 4.927
Grimsley 4.876
Solomon 5.016
Lehninger 4.978
Nozaki 5.143
DTASelect 5.334
Thurlkill 4.965
EMBOSS 4.965
Sillero 5.207
Patrickios 4.126
IPC_peptide 5.029
IPC2_peptide 5.207
IPC2.peptide.svr19 5.198
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
142
142
142
142.0
16.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.817 ± 0.0
2.113 ± 0.0
8.451 ± 0.0
6.338 ± 0.0
4.225 ± 0.0
4.225 ± 0.0
2.113 ± 0.0
15.493 ± 0.0
6.338 ± 0.0
6.338 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
6.338 ± 0.0
6.338 ± 0.0
4.225 ± 0.0
2.817 ± 0.0
4.93 ± 0.0
4.93 ± 0.0
5.634 ± 0.0
3.521 ± 0.0
0.0 ± 0.0
2.817 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here