Amino acid dipepetide frequency for Arthrobacter phage Daiboju

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.196AlaAla: 12.196 ± 1.211
0.717AlaCys: 0.717 ± 0.282
5.452AlaAsp: 5.452 ± 0.568
6.887AlaGlu: 6.887 ± 0.81
2.583AlaPhe: 2.583 ± 0.46
8.394AlaGly: 8.394 ± 1.024
1.076AlaHis: 1.076 ± 0.268
5.452AlaIle: 5.452 ± 0.816
5.811AlaLys: 5.811 ± 0.879
8.896AlaLeu: 8.896 ± 1.148
2.583AlaMet: 2.583 ± 0.475
3.802AlaAsn: 3.802 ± 0.453
4.448AlaPro: 4.448 ± 0.598
4.448AlaGln: 4.448 ± 0.616
5.883AlaArg: 5.883 ± 0.774
6.457AlaSer: 6.457 ± 0.66
6.385AlaThr: 6.385 ± 0.691
6.098AlaVal: 6.098 ± 0.632
1.937AlaTrp: 1.937 ± 0.426
3.085AlaTyr: 3.085 ± 0.51
0.0AlaXaa: 0.0 ± 0.0
Cys
0.43CysAla: 0.43 ± 0.177
0.0CysCys: 0.0 ± 0.0
0.502CysAsp: 0.502 ± 0.204
0.574CysGlu: 0.574 ± 0.228
0.215CysPhe: 0.215 ± 0.114
1.22CysGly: 1.22 ± 0.383
0.215CysHis: 0.215 ± 0.108
0.43CysIle: 0.43 ± 0.16
0.215CysLys: 0.215 ± 0.114
0.359CysLeu: 0.359 ± 0.137
0.0CysMet: 0.0 ± 0.0
0.143CysAsn: 0.143 ± 0.102
0.359CysPro: 0.359 ± 0.154
0.502CysGln: 0.502 ± 0.172
0.502CysArg: 0.502 ± 0.196
0.359CysSer: 0.359 ± 0.165
0.43CysThr: 0.43 ± 0.199
0.215CysVal: 0.215 ± 0.114
0.359CysTrp: 0.359 ± 0.176
0.215CysTyr: 0.215 ± 0.112
0.0CysXaa: 0.0 ± 0.0
Asp
6.098AspAla: 6.098 ± 0.514
0.502AspCys: 0.502 ± 0.199
3.3AspAsp: 3.3 ± 0.47
4.95AspGlu: 4.95 ± 0.678
1.865AspPhe: 1.865 ± 0.389
5.309AspGly: 5.309 ± 0.63
1.076AspHis: 1.076 ± 0.386
2.224AspIle: 2.224 ± 0.34
2.009AspLys: 2.009 ± 0.405
5.739AspLeu: 5.739 ± 0.631
1.291AspMet: 1.291 ± 0.285
1.363AspAsn: 1.363 ± 0.284
3.515AspPro: 3.515 ± 0.53
2.224AspGln: 2.224 ± 0.51
3.731AspArg: 3.731 ± 0.542
3.228AspSer: 3.228 ± 0.528
3.444AspThr: 3.444 ± 0.556
2.654AspVal: 2.654 ± 0.413
1.148AspTrp: 1.148 ± 0.259
1.794AspTyr: 1.794 ± 0.379
0.0AspXaa: 0.0 ± 0.0
Glu
7.031GluAla: 7.031 ± 0.878
0.646GluCys: 0.646 ± 0.236
4.018GluAsp: 4.018 ± 0.769
4.089GluGlu: 4.089 ± 0.538
2.367GluPhe: 2.367 ± 0.468
6.457GluGly: 6.457 ± 0.584
1.291GluHis: 1.291 ± 0.304
2.439GluIle: 2.439 ± 0.4
3.085GluLys: 3.085 ± 0.533
5.955GluLeu: 5.955 ± 0.679
1.435GluMet: 1.435 ± 0.33
2.08GluAsn: 2.08 ± 0.319
2.511GluPro: 2.511 ± 0.452
3.157GluGln: 3.157 ± 0.541
4.233GluArg: 4.233 ± 0.602
2.798GluSer: 2.798 ± 0.409
3.587GluThr: 3.587 ± 0.479
4.448GluVal: 4.448 ± 0.649
1.435GluTrp: 1.435 ± 0.314
1.22GluTyr: 1.22 ± 0.265
0.0GluXaa: 0.0 ± 0.0
Phe
2.87PheAla: 2.87 ± 0.622
0.143PheCys: 0.143 ± 0.09
2.583PheAsp: 2.583 ± 0.416
2.08PheGlu: 2.08 ± 0.371
1.578PhePhe: 1.578 ± 0.469
3.157PheGly: 3.157 ± 0.569
0.646PheHis: 0.646 ± 0.169
1.004PheIle: 1.004 ± 0.34
1.507PheLys: 1.507 ± 0.279
1.722PheLeu: 1.722 ± 0.271
0.861PheMet: 0.861 ± 0.296
1.291PheAsn: 1.291 ± 0.348
0.574PhePro: 0.574 ± 0.2
1.363PheGln: 1.363 ± 0.318
2.224PheArg: 2.224 ± 0.383
1.865PheSer: 1.865 ± 0.572
2.009PheThr: 2.009 ± 0.468
1.794PheVal: 1.794 ± 0.544
0.215PheTrp: 0.215 ± 0.11
0.789PheTyr: 0.789 ± 0.277
0.0PheXaa: 0.0 ± 0.0
Gly
8.394GlyAla: 8.394 ± 1.089
0.646GlyCys: 0.646 ± 0.208
4.52GlyAsp: 4.52 ± 0.639
5.668GlyGlu: 5.668 ± 0.65
3.228GlyPhe: 3.228 ± 0.679
5.955GlyGly: 5.955 ± 0.614
2.152GlyHis: 2.152 ± 0.474
4.376GlyIle: 4.376 ± 0.682
3.587GlyLys: 3.587 ± 0.387
7.246GlyLeu: 7.246 ± 0.833
2.08GlyMet: 2.08 ± 0.404
4.161GlyAsn: 4.161 ± 0.734
4.089GlyPro: 4.089 ± 0.615
3.515GlyGln: 3.515 ± 0.518
4.95GlyArg: 4.95 ± 0.629
6.313GlySer: 6.313 ± 0.737
5.883GlyThr: 5.883 ± 0.892
7.533GlyVal: 7.533 ± 0.804
1.794GlyTrp: 1.794 ± 0.427
2.296GlyTyr: 2.296 ± 0.458
0.0GlyXaa: 0.0 ± 0.0
His
1.865HisAla: 1.865 ± 0.421
0.215HisCys: 0.215 ± 0.128
0.861HisAsp: 0.861 ± 0.25
0.789HisGlu: 0.789 ± 0.262
0.646HisPhe: 0.646 ± 0.214
1.937HisGly: 1.937 ± 0.31
0.502HisHis: 0.502 ± 0.194
1.363HisIle: 1.363 ± 0.347
0.861HisLys: 0.861 ± 0.231
1.794HisLeu: 1.794 ± 0.433
0.502HisMet: 0.502 ± 0.171
0.861HisAsn: 0.861 ± 0.218
0.717HisPro: 0.717 ± 0.221
0.574HisGln: 0.574 ± 0.196
0.646HisArg: 0.646 ± 0.228
1.004HisSer: 1.004 ± 0.23
1.148HisThr: 1.148 ± 0.305
0.789HisVal: 0.789 ± 0.243
0.43HisTrp: 0.43 ± 0.184
1.148HisTyr: 1.148 ± 0.36
0.0HisXaa: 0.0 ± 0.0
Ile
4.735IleAla: 4.735 ± 0.676
0.215IleCys: 0.215 ± 0.132
2.87IleAsp: 2.87 ± 0.453
2.87IleGlu: 2.87 ± 0.452
1.148IlePhe: 1.148 ± 0.347
3.874IleGly: 3.874 ± 0.579
0.861IleHis: 0.861 ± 0.241
2.009IleIle: 2.009 ± 0.648
2.654IleLys: 2.654 ± 0.458
3.659IleLeu: 3.659 ± 0.52
0.933IleMet: 0.933 ± 0.375
2.224IleAsn: 2.224 ± 0.371
1.794IlePro: 1.794 ± 0.433
2.798IleGln: 2.798 ± 0.309
3.444IleArg: 3.444 ± 0.446
3.731IleSer: 3.731 ± 0.718
3.013IleThr: 3.013 ± 0.475
3.3IleVal: 3.3 ± 0.623
0.646IleTrp: 0.646 ± 0.195
0.933IleTyr: 0.933 ± 0.208
0.0IleXaa: 0.0 ± 0.0
Lys
5.883LysAla: 5.883 ± 0.873
0.574LysCys: 0.574 ± 0.212
2.798LysAsp: 2.798 ± 0.571
2.654LysGlu: 2.654 ± 0.438
1.722LysPhe: 1.722 ± 0.362
4.089LysGly: 4.089 ± 0.523
1.435LysHis: 1.435 ± 0.369
2.654LysIle: 2.654 ± 0.437
2.08LysLys: 2.08 ± 0.443
4.663LysLeu: 4.663 ± 0.65
1.291LysMet: 1.291 ± 0.298
0.717LysAsn: 0.717 ± 0.191
2.798LysPro: 2.798 ± 0.474
1.578LysGln: 1.578 ± 0.288
2.511LysArg: 2.511 ± 0.398
2.726LysSer: 2.726 ± 0.512
2.941LysThr: 2.941 ± 0.459
3.802LysVal: 3.802 ± 0.598
0.502LysTrp: 0.502 ± 0.188
1.22LysTyr: 1.22 ± 0.24
0.0LysXaa: 0.0 ± 0.0
Leu
9.039LeuAla: 9.039 ± 0.797
0.646LeuCys: 0.646 ± 0.265
5.739LeuAsp: 5.739 ± 0.515
4.089LeuGlu: 4.089 ± 0.684
2.439LeuPhe: 2.439 ± 0.435
8.322LeuGly: 8.322 ± 1.045
1.507LeuHis: 1.507 ± 0.366
4.233LeuIle: 4.233 ± 0.449
2.941LeuLys: 2.941 ± 0.468
7.031LeuLeu: 7.031 ± 0.794
1.794LeuMet: 1.794 ± 0.416
3.444LeuAsn: 3.444 ± 0.555
5.022LeuPro: 5.022 ± 0.64
3.372LeuGln: 3.372 ± 0.353
5.811LeuArg: 5.811 ± 0.572
4.233LeuSer: 4.233 ± 0.74
5.094LeuThr: 5.094 ± 0.463
6.457LeuVal: 6.457 ± 0.658
1.507LeuTrp: 1.507 ± 0.304
2.009LeuTyr: 2.009 ± 0.345
0.0LeuXaa: 0.0 ± 0.0
Met
2.798MetAla: 2.798 ± 0.426
0.072MetCys: 0.072 ± 0.067
1.076MetAsp: 1.076 ± 0.39
1.722MetGlu: 1.722 ± 0.307
0.287MetPhe: 0.287 ± 0.115
1.363MetGly: 1.363 ± 0.328
0.287MetHis: 0.287 ± 0.139
1.794MetIle: 1.794 ± 0.386
1.22MetLys: 1.22 ± 0.313
1.22MetLeu: 1.22 ± 0.245
0.359MetMet: 0.359 ± 0.173
1.004MetAsn: 1.004 ± 0.243
1.937MetPro: 1.937 ± 0.402
0.502MetGln: 0.502 ± 0.257
0.646MetArg: 0.646 ± 0.232
1.794MetSer: 1.794 ± 0.291
1.65MetThr: 1.65 ± 0.429
2.511MetVal: 2.511 ± 0.403
0.359MetTrp: 0.359 ± 0.181
0.574MetTyr: 0.574 ± 0.223
0.0MetXaa: 0.0 ± 0.0
Asn
2.87AsnAla: 2.87 ± 0.318
0.359AsnCys: 0.359 ± 0.141
1.578AsnAsp: 1.578 ± 0.368
1.722AsnGlu: 1.722 ± 0.323
0.933AsnPhe: 0.933 ± 0.281
4.161AsnGly: 4.161 ± 0.67
0.861AsnHis: 0.861 ± 0.327
1.363AsnIle: 1.363 ± 0.276
1.865AsnLys: 1.865 ± 0.411
3.085AsnLeu: 3.085 ± 0.42
0.502AsnMet: 0.502 ± 0.182
1.363AsnAsn: 1.363 ± 0.421
1.937AsnPro: 1.937 ± 0.42
1.578AsnGln: 1.578 ± 0.321
1.794AsnArg: 1.794 ± 0.38
1.794AsnSer: 1.794 ± 0.333
2.87AsnThr: 2.87 ± 0.422
3.085AsnVal: 3.085 ± 0.485
1.076AsnTrp: 1.076 ± 0.261
1.076AsnTyr: 1.076 ± 0.239
0.0AsnXaa: 0.0 ± 0.0
Pro
5.524ProAla: 5.524 ± 0.71
0.43ProCys: 0.43 ± 0.187
2.941ProAsp: 2.941 ± 0.45
3.3ProGlu: 3.3 ± 0.483
1.076ProPhe: 1.076 ± 0.301
4.878ProGly: 4.878 ± 0.673
0.502ProHis: 0.502 ± 0.212
2.152ProIle: 2.152 ± 0.397
3.013ProLys: 3.013 ± 0.476
2.798ProLeu: 2.798 ± 0.349
1.291ProMet: 1.291 ± 0.324
1.578ProAsn: 1.578 ± 0.306
1.435ProPro: 1.435 ± 0.274
1.076ProGln: 1.076 ± 0.231
2.367ProArg: 2.367 ± 0.434
3.946ProSer: 3.946 ± 0.53
3.587ProThr: 3.587 ± 0.75
5.094ProVal: 5.094 ± 0.7
1.507ProTrp: 1.507 ± 0.358
0.574ProTyr: 0.574 ± 0.227
0.0ProXaa: 0.0 ± 0.0
Gln
3.587GlnAla: 3.587 ± 0.488
0.143GlnCys: 0.143 ± 0.1
2.152GlnAsp: 2.152 ± 0.438
2.439GlnGlu: 2.439 ± 0.402
1.291GlnPhe: 1.291 ± 0.272
2.798GlnGly: 2.798 ± 0.475
0.502GlnHis: 0.502 ± 0.14
1.865GlnIle: 1.865 ± 0.357
2.08GlnLys: 2.08 ± 0.298
5.094GlnLeu: 5.094 ± 0.776
1.22GlnMet: 1.22 ± 0.261
1.65GlnAsn: 1.65 ± 0.354
2.009GlnPro: 2.009 ± 0.376
1.937GlnGln: 1.937 ± 0.391
2.009GlnArg: 2.009 ± 0.416
1.578GlnSer: 1.578 ± 0.305
2.152GlnThr: 2.152 ± 0.326
3.444GlnVal: 3.444 ± 0.42
1.004GlnTrp: 1.004 ± 0.232
0.861GlnTyr: 0.861 ± 0.262
0.0GlnXaa: 0.0 ± 0.0
Arg
5.739ArgAla: 5.739 ± 0.711
0.502ArgCys: 0.502 ± 0.203
3.659ArgAsp: 3.659 ± 0.47
3.587ArgGlu: 3.587 ± 0.538
1.65ArgPhe: 1.65 ± 0.373
3.659ArgGly: 3.659 ± 0.455
1.076ArgHis: 1.076 ± 0.354
3.228ArgIle: 3.228 ± 0.479
4.089ArgLys: 4.089 ± 0.631
5.739ArgLeu: 5.739 ± 0.678
1.435ArgMet: 1.435 ± 0.379
2.296ArgAsn: 2.296 ± 0.414
2.798ArgPro: 2.798 ± 0.524
2.367ArgGln: 2.367 ± 0.391
3.731ArgArg: 3.731 ± 0.587
3.085ArgSer: 3.085 ± 0.398
3.085ArgThr: 3.085 ± 0.416
3.802ArgVal: 3.802 ± 0.517
1.004ArgTrp: 1.004 ± 0.342
1.435ArgTyr: 1.435 ± 0.406
0.0ArgXaa: 0.0 ± 0.0
Ser
4.376SerAla: 4.376 ± 0.598
0.43SerCys: 0.43 ± 0.186
3.228SerAsp: 3.228 ± 0.477
4.807SerGlu: 4.807 ± 0.498
1.65SerPhe: 1.65 ± 0.337
8.035SerGly: 8.035 ± 0.984
1.148SerHis: 1.148 ± 0.253
3.515SerIle: 3.515 ± 0.478
2.367SerLys: 2.367 ± 0.38
5.022SerLeu: 5.022 ± 0.795
1.435SerMet: 1.435 ± 0.436
1.65SerAsn: 1.65 ± 0.351
3.013SerPro: 3.013 ± 0.443
1.65SerGln: 1.65 ± 0.346
2.87SerArg: 2.87 ± 0.477
3.444SerSer: 3.444 ± 0.524
4.304SerThr: 4.304 ± 0.549
4.807SerVal: 4.807 ± 0.563
1.076SerTrp: 1.076 ± 0.393
2.296SerTyr: 2.296 ± 0.398
0.0SerXaa: 0.0 ± 0.0
Thr
7.533ThrAla: 7.533 ± 0.97
0.359ThrCys: 0.359 ± 0.17
3.3ThrAsp: 3.3 ± 0.528
4.448ThrGlu: 4.448 ± 0.437
2.009ThrPhe: 2.009 ± 0.414
5.094ThrGly: 5.094 ± 1.057
0.933ThrHis: 0.933 ± 0.211
2.367ThrIle: 2.367 ± 0.444
3.085ThrLys: 3.085 ± 0.491
5.022ThrLeu: 5.022 ± 0.695
1.22ThrMet: 1.22 ± 0.317
2.152ThrAsn: 2.152 ± 0.331
3.587ThrPro: 3.587 ± 0.551
2.439ThrGln: 2.439 ± 0.432
3.515ThrArg: 3.515 ± 0.55
4.735ThrSer: 4.735 ± 0.557
5.452ThrThr: 5.452 ± 0.919
4.376ThrVal: 4.376 ± 0.612
0.933ThrTrp: 0.933 ± 0.288
1.722ThrTyr: 1.722 ± 0.419
0.0ThrXaa: 0.0 ± 0.0
Val
6.6ValAla: 6.6 ± 0.745
0.287ValCys: 0.287 ± 0.13
3.874ValAsp: 3.874 ± 0.48
4.878ValGlu: 4.878 ± 0.522
2.009ValPhe: 2.009 ± 0.498
5.739ValGly: 5.739 ± 0.781
1.578ValHis: 1.578 ± 0.317
3.444ValIle: 3.444 ± 0.595
3.874ValLys: 3.874 ± 0.562
5.883ValLeu: 5.883 ± 0.645
2.224ValMet: 2.224 ± 0.354
2.726ValAsn: 2.726 ± 0.4
4.304ValPro: 4.304 ± 0.492
3.587ValGln: 3.587 ± 0.453
3.946ValArg: 3.946 ± 0.371
5.237ValSer: 5.237 ± 0.53
3.946ValThr: 3.946 ± 0.506
4.735ValVal: 4.735 ± 0.634
1.937ValTrp: 1.937 ± 0.353
1.722ValTyr: 1.722 ± 0.387
0.0ValXaa: 0.0 ± 0.0
Trp
2.726TrpAla: 2.726 ± 0.479
0.072TrpCys: 0.072 ± 0.071
1.363TrpAsp: 1.363 ± 0.403
1.004TrpGlu: 1.004 ± 0.323
0.789TrpPhe: 0.789 ± 0.23
1.435TrpGly: 1.435 ± 0.349
0.359TrpHis: 0.359 ± 0.151
0.861TrpIle: 0.861 ± 0.218
1.004TrpLys: 1.004 ± 0.258
1.865TrpLeu: 1.865 ± 0.381
0.43TrpMet: 0.43 ± 0.164
0.646TrpAsn: 0.646 ± 0.254
0.789TrpPro: 0.789 ± 0.343
0.43TrpGln: 0.43 ± 0.176
1.004TrpArg: 1.004 ± 0.316
1.578TrpSer: 1.578 ± 0.359
1.148TrpThr: 1.148 ± 0.3
1.937TrpVal: 1.937 ± 0.288
0.717TrpTrp: 0.717 ± 0.22
0.287TrpTyr: 0.287 ± 0.136
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.296TyrAla: 2.296 ± 0.38
0.287TyrCys: 0.287 ± 0.153
1.65TyrAsp: 1.65 ± 0.258
1.722TyrGlu: 1.722 ± 0.337
0.789TyrPhe: 0.789 ± 0.225
2.511TyrGly: 2.511 ± 0.479
0.646TyrHis: 0.646 ± 0.2
0.933TyrIle: 0.933 ± 0.243
1.291TyrLys: 1.291 ± 0.294
1.937TyrLeu: 1.937 ± 0.373
0.215TyrMet: 0.215 ± 0.135
0.717TyrAsn: 0.717 ± 0.258
1.435TyrPro: 1.435 ± 0.326
0.717TyrGln: 0.717 ± 0.255
2.152TyrArg: 2.152 ± 0.311
1.291TyrSer: 1.291 ± 0.314
2.152TyrThr: 2.152 ± 0.407
1.65TyrVal: 1.65 ± 0.274
0.861TyrTrp: 0.861 ± 0.231
0.502TyrTyr: 0.502 ± 0.171
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 60 proteins (13940 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski