Invertebrate iridescent virus 6 (IIV-6) (Chilo iridescent virus)
Average proteome isoelectric point is 7.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 468 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q91F62|Q91F62_IIV6 464R OS=Invertebrate iridescent virus 6 OX=176652 PE=4 SV=1
MM1 pKa = 7.89 DD2 pKa = 6.41 AIFYY6 pKa = 7.34 PLPIGEE12 pKa = 4.38 TGDD15 pKa = 3.47 NEE17 pKa = 4.62 KK18 pKa = 10.92 YY19 pKa = 10.91 LEE21 pKa = 4.61 DD22 pKa = 3.83 FQEE25 pKa = 4.02 EE26 pKa = 4.43 DD27 pKa = 3.5 FQEE30 pKa = 4.1 EE31 pKa = 4.4 DD32 pKa = 3.48 FQEE35 pKa = 4.1 EE36 pKa = 4.4 DD37 pKa = 3.48 FQEE40 pKa = 4.1 EE41 pKa = 4.39 DD42 pKa = 3.57 FQEE45 pKa = 4.15 EE46 pKa = 4.47 DD47 pKa = 3.31 EE48 pKa = 4.93 DD49 pKa = 4.97 EE50 pKa = 4.35 EE51 pKa = 4.88 DD52 pKa = 4.09 EE53 pKa = 4.7 EE54 pKa = 4.81 VNEE57 pKa = 4.24 YY58 pKa = 10.4 PSDD61 pKa = 4.45 LDD63 pKa = 5.18 DD64 pKa = 5.23 EE65 pKa = 5.3 YY66 pKa = 10.89 PDD68 pKa = 3.16 SDD70 pKa = 4.49 YY71 pKa = 11.61 YY72 pKa = 10.99 DD73 pKa = 3.73 EE74 pKa = 5.96 RR75 pKa = 11.84 SDD77 pKa = 4.48 RR78 pKa = 11.84 HH79 pKa = 5.63 WSDD82 pKa = 4.15 DD83 pKa = 4.22 DD84 pKa = 4.42 SDD86 pKa = 5.29 RR87 pKa = 11.84 DD88 pKa = 4.11 LDD90 pKa = 4.03 DD91 pKa = 6.41 LYY93 pKa = 11.47 DD94 pKa = 3.81 EE95 pKa = 4.61 YY96 pKa = 11.47 EE97 pKa = 3.93 NYY99 pKa = 10.21 YY100 pKa = 10.99 EE101 pKa = 3.99 EE102 pKa = 4.59 VQVKK106 pKa = 9.4 KK107 pKa = 10.83 QNLEE111 pKa = 3.83 VEE113 pKa = 4.9 IIDD116 pKa = 3.4 ITPEE120 pKa = 4.14 IEE122 pKa = 4.31 ILNQNEE128 pKa = 4.2 KK129 pKa = 10.92 EE130 pKa = 4.19 KK131 pKa = 10.94 FNQVEE136 pKa = 4.41 DD137 pKa = 3.8 KK138 pKa = 10.67 LTTLSKK144 pKa = 11.01 NLTCIICLTNQVQILTIPCGHH165 pKa = 7.83 LIMCNPCSLNLNNSVCTRR183 pKa = 11.84 GVNSNYY189 pKa = 9.41 EE190 pKa = 4.04 KK191 pKa = 10.67 CPKK194 pKa = 9.99 CRR196 pKa = 11.84 TPIYY200 pKa = 10.18 NKK202 pKa = 10.17 IIARR206 pKa = 11.84 LPP208 pKa = 3.51
Molecular weight: 24.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.735
IPC2_protein 3.783
IPC_protein 3.77
Toseland 3.567
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.643
Rodwell 3.592
Grimsley 3.478
Solomon 3.732
Lehninger 3.694
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.605
EMBOSS 3.656
Sillero 3.884
Patrickios 0.795
IPC_peptide 3.732
IPC2_peptide 3.872
IPC2.peptide.svr19 3.788
Protein with the highest isoelectric point:
>sp|O55725|107L_IIV6 Uncharacterized protein 107L OS=Invertebrate iridescent virus 6 OX=176652 GN=IIV6-107L PE=4 SV=1
MM1 pKa = 7.8 PICVLRR7 pKa = 11.84 KK8 pKa = 8.41 SPKK11 pKa = 9.43 RR12 pKa = 11.84 RR13 pKa = 11.84 SPSPKK18 pKa = 8.89 RR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 SPKK24 pKa = 8.61 RR25 pKa = 11.84 RR26 pKa = 11.84 SPRR29 pKa = 11.84 RR30 pKa = 11.84 SPRR33 pKa = 11.84 RR34 pKa = 11.84 SPRR37 pKa = 11.84 RR38 pKa = 11.84 SPPRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 SPRR47 pKa = 11.84 RR48 pKa = 11.84 STRR51 pKa = 11.84 RR52 pKa = 11.84 SPRR55 pKa = 11.84 RR56 pKa = 11.84 RR57 pKa = 3.32
Molecular weight: 7.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.618
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.325
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.225
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
464
4
468
77551
40
2432
165.7
19.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.739 ± 0.282
1.881 ± 0.099
4.793 ± 0.131
5.919 ± 0.242
5.571 ± 0.196
4.716 ± 0.276
1.877 ± 0.086
8.814 ± 0.132
8.683 ± 0.299
9.729 ± 0.133
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.605 ± 0.082
7.132 ± 0.131
3.883 ± 0.126
3.484 ± 0.09
3.462 ± 0.161
7.723 ± 0.287
5.791 ± 0.297
5.362 ± 0.113
0.896 ± 0.047
3.941 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here