Prevotella sp. CAG:520

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; environmental samples

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2171 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6F562|R6F562_9BACT Uncharacterized protein OS=Prevotella sp. CAG:520 OX=1262929 GN=BN691_01426 PE=4 SV=1
MM1 pKa = 7.36SNEE4 pKa = 3.79VKK6 pKa = 10.71YY7 pKa = 10.78ISYY10 pKa = 10.67EE11 pKa = 3.85EE12 pKa = 4.78AINVYY17 pKa = 10.8NKK19 pKa = 10.14MIDD22 pKa = 3.34ASEE25 pKa = 4.39GGFDD29 pKa = 3.94GVRR32 pKa = 11.84DD33 pKa = 3.71EE34 pKa = 4.94GGILATLDD42 pKa = 3.56FVQNDD47 pKa = 3.53MYY49 pKa = 11.66YY50 pKa = 10.21PDD52 pKa = 5.18FSDD55 pKa = 3.27KK56 pKa = 10.98LSYY59 pKa = 10.96LVFKK63 pKa = 10.23FCSGHH68 pKa = 5.71YY69 pKa = 10.55FNDD72 pKa = 3.58GNKK75 pKa = 10.06RR76 pKa = 11.84IALTLGAYY84 pKa = 9.85FLYY87 pKa = 10.5KK88 pKa = 10.42NSYY91 pKa = 7.63VWQATIFMRR100 pKa = 11.84QMEE103 pKa = 4.99SIVYY107 pKa = 9.24HH108 pKa = 5.64VAASNIDD115 pKa = 3.22QDD117 pKa = 4.09LLLRR121 pKa = 11.84IMSFFMKK128 pKa = 10.75GEE130 pKa = 4.45DD131 pKa = 3.63YY132 pKa = 11.32DD133 pKa = 4.27EE134 pKa = 4.2EE135 pKa = 4.44LKK137 pKa = 10.42IDD139 pKa = 3.61IANAMSNGDD148 pKa = 4.0LGIKK152 pKa = 10.49GEE154 pKa = 4.48DD155 pKa = 3.63YY156 pKa = 10.95EE157 pKa = 5.32ITDD160 pKa = 3.83EE161 pKa = 5.38DD162 pKa = 5.29DD163 pKa = 3.5NFF165 pKa = 4.48

Molecular weight: 19.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6F6B7|R6F6B7_9BACT Uncharacterized protein OS=Prevotella sp. CAG:520 OX=1262929 GN=BN691_01334 PE=4 SV=1
MM1 pKa = 7.85PNGKK5 pKa = 9.19KK6 pKa = 10.25KK7 pKa = 10.12KK8 pKa = 7.0GHH10 pKa = 6.14KK11 pKa = 9.06MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2171

0

2171

780981

29

2655

359.7

40.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.013 ± 0.053

1.352 ± 0.021

5.974 ± 0.036

6.239 ± 0.053

4.306 ± 0.033

6.883 ± 0.049

1.967 ± 0.023

6.135 ± 0.05

6.475 ± 0.049

8.547 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.92 ± 0.023

5.041 ± 0.043

3.558 ± 0.023

3.421 ± 0.028

4.847 ± 0.039

5.986 ± 0.047

5.917 ± 0.042

6.979 ± 0.044

1.207 ± 0.02

4.233 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski