Amino acid dipepetide frequency for Prevotella sp. CAG:520

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.325AlaAla: 7.325 ± 0.118	1.078AlaCys: 1.078 ± 0.039	5.387AlaAsp: 5.387 ± 0.092	5.575AlaGlu: 5.575 ± 0.106	3.266AlaPhe: 3.266 ± 0.078	5.044AlaGly: 5.044 ± 0.09	1.455AlaHis: 1.455 ± 0.046	4.626AlaIle: 4.626 ± 0.083	4.918AlaLys: 4.918 ± 0.08	7.383AlaLeu: 7.383 ± 0.124	2.392AlaMet: 2.392 ± 0.06	3.506AlaAsn: 3.506 ± 0.067	2.579AlaPro: 2.579 ± 0.071	3.243AlaGln: 3.243 ± 0.059	3.428AlaArg: 3.428 ± 0.074	4.672AlaSer: 4.672 ± 0.079	4.935AlaThr: 4.935 ± 0.1	5.452AlaVal: 5.452 ± 0.093	0.862AlaTrp: 0.862 ± 0.036	3.004AlaTyr: 3.004 ± 0.069	0.003AlaXaa: 0.003 ± 0.002
Cys	0.955CysAla: 0.955 ± 0.041	0.243CysCys: 0.243 ± 0.018	0.816CysAsp: 0.816 ± 0.036	0.776CysGlu: 0.776 ± 0.034	0.601CysPhe: 0.601 ± 0.028	1.216CysGly: 1.216 ± 0.048	0.312CysHis: 0.312 ± 0.018	0.787CysIle: 0.787 ± 0.037	0.753CysLys: 0.753 ± 0.035	1.138CysLeu: 1.138 ± 0.037	0.366CysMet: 0.366 ± 0.022	0.652CysAsn: 0.652 ± 0.029	0.533CysPro: 0.533 ± 0.027	0.384CysGln: 0.384 ± 0.022	0.709CysArg: 0.709 ± 0.028	0.845CysSer: 0.845 ± 0.037	0.761CysThr: 0.761 ± 0.028	0.908CysVal: 0.908 ± 0.041	0.159CysTrp: 0.159 ± 0.015	0.604CysTyr: 0.604 ± 0.029	0.0CysXaa: 0.0 ± 0.0
Asp	4.852AspAla: 4.852 ± 0.084	0.759AspCys: 0.759 ± 0.039	3.437AspAsp: 3.437 ± 0.078	4.218AspGlu: 4.218 ± 0.086	3.051AspPhe: 3.051 ± 0.065	4.963AspGly: 4.963 ± 0.107	0.99AspHis: 0.99 ± 0.033	4.06AspIle: 4.06 ± 0.083	3.68AspLys: 3.68 ± 0.072	4.45AspLeu: 4.45 ± 0.081	1.816AspMet: 1.816 ± 0.042	3.224AspAsn: 3.224 ± 0.072	1.918AspPro: 1.918 ± 0.047	1.37AspGln: 1.37 ± 0.043	2.781AspArg: 2.781 ± 0.063	3.143AspSer: 3.143 ± 0.072	2.981AspThr: 2.981 ± 0.063	4.26AspVal: 4.26 ± 0.08	0.86AspTrp: 0.86 ± 0.034	2.926AspTyr: 2.926 ± 0.061	0.0AspXaa: 0.0 ± 0.0
Glu	5.113GluAla: 5.113 ± 0.104	0.739GluCys: 0.739 ± 0.033	3.214GluAsp: 3.214 ± 0.072	4.457GluGlu: 4.457 ± 0.099	2.503GluPhe: 2.503 ± 0.057	4.261GluGly: 4.261 ± 0.083	1.434GluHis: 1.434 ± 0.045	3.741GluIle: 3.741 ± 0.086	4.492GluLys: 4.492 ± 0.081	5.395GluLeu: 5.395 ± 0.096	2.038GluMet: 2.038 ± 0.053	3.204GluAsn: 3.204 ± 0.063	1.876GluPro: 1.876 ± 0.045	2.721GluGln: 2.721 ± 0.062	3.388GluArg: 3.388 ± 0.071	2.995GluSer: 2.995 ± 0.062	3.243GluThr: 3.243 ± 0.066	4.044GluVal: 4.044 ± 0.086	0.826GluTrp: 0.826 ± 0.033	2.702GluTyr: 2.702 ± 0.064	0.0GluXaa: 0.0 ± 0.0
Phe	3.418PheAla: 3.418 ± 0.072	0.75PheCys: 0.75 ± 0.033	2.967PheAsp: 2.967 ± 0.056	2.415PheGlu: 2.415 ± 0.056	2.01PhePhe: 2.01 ± 0.06	3.282PheGly: 3.282 ± 0.065	0.832PheHis: 0.832 ± 0.031	2.579PheIle: 2.579 ± 0.066	2.384PheLys: 2.384 ± 0.055	3.321PheLeu: 3.321 ± 0.075	1.22PheMet: 1.22 ± 0.04	2.264PheAsn: 2.264 ± 0.061	1.391PhePro: 1.391 ± 0.048	1.046PheGln: 1.046 ± 0.036	2.056PheArg: 2.056 ± 0.061	3.141PheSer: 3.141 ± 0.064	2.586PheThr: 2.586 ± 0.061	3.15PheVal: 3.15 ± 0.075	0.472PheTrp: 0.472 ± 0.029	1.771PheTyr: 1.771 ± 0.049	0.001PheXaa: 0.001 ± 0.001
Gly	5.16GlyAla: 5.16 ± 0.092	1.037GlyCys: 1.037 ± 0.04	3.973GlyAsp: 3.973 ± 0.077	4.359GlyGlu: 4.359 ± 0.086	3.008GlyPhe: 3.008 ± 0.063	4.995GlyGly: 4.995 ± 0.096	1.207GlyHis: 1.207 ± 0.036	4.596GlyIle: 4.596 ± 0.087	5.386GlyLys: 5.386 ± 0.08	5.448GlyLeu: 5.448 ± 0.099	2.127GlyMet: 2.127 ± 0.064	3.48GlyAsn: 3.48 ± 0.078	1.237GlyPro: 1.237 ± 0.036	1.837GlyGln: 1.837 ± 0.052	3.012GlyArg: 3.012 ± 0.072	4.101GlySer: 4.101 ± 0.091	4.291GlyThr: 4.291 ± 0.093	5.46GlyVal: 5.46 ± 0.087	0.921GlyTrp: 0.921 ± 0.042	3.2GlyTyr: 3.2 ± 0.072	0.0GlyXaa: 0.0 ± 0.0
His	1.342HisAla: 1.342 ± 0.045	0.292HisCys: 0.292 ± 0.016	1.19HisAsp: 1.19 ± 0.041	1.079HisGlu: 1.079 ± 0.041	0.987HisPhe: 0.987 ± 0.043	1.361HisGly: 1.361 ± 0.044	0.535HisHis: 0.535 ± 0.031	1.43HisIle: 1.43 ± 0.038	1.113HisLys: 1.113 ± 0.04	1.647HisLeu: 1.647 ± 0.041	0.388HisMet: 0.388 ± 0.022	1.087HisAsn: 1.087 ± 0.03	0.954HisPro: 0.954 ± 0.043	0.565HisGln: 0.565 ± 0.031	1.009HisArg: 1.009 ± 0.033	1.159HisSer: 1.159 ± 0.041	1.091HisThr: 1.091 ± 0.035	1.256HisVal: 1.256 ± 0.039	0.237HisTrp: 0.237 ± 0.018	0.951HisTyr: 0.951 ± 0.038	0.0HisXaa: 0.0 ± 0.0
Ile	5.383IleAla: 5.383 ± 0.098	0.907IleCys: 0.907 ± 0.034	4.384IleAsp: 4.384 ± 0.083	4.032IleGlu: 4.032 ± 0.082	2.292IlePhe: 2.292 ± 0.063	4.304IleGly: 4.304 ± 0.076	1.163IleHis: 1.163 ± 0.038	3.781IleIle: 3.781 ± 0.094	3.834IleLys: 3.834 ± 0.08	4.53IleLeu: 4.53 ± 0.096	1.519IleMet: 1.519 ± 0.044	3.123IleAsn: 3.123 ± 0.068	2.42IlePro: 2.42 ± 0.062	1.597IleGln: 1.597 ± 0.047	2.764IleArg: 2.764 ± 0.068	3.948IleSer: 3.948 ± 0.079	3.749IleThr: 3.749 ± 0.077	4.706IleVal: 4.706 ± 0.089	0.56IleTrp: 0.56 ± 0.026	2.355IleTyr: 2.355 ± 0.064	0.0IleXaa: 0.0 ± 0.0
Lys	5.306LysAla: 5.306 ± 0.094	0.618LysCys: 0.618 ± 0.026	3.726LysAsp: 3.726 ± 0.069	4.578LysGlu: 4.578 ± 0.095	2.315LysPhe: 2.315 ± 0.051	4.264LysGly: 4.264 ± 0.09	1.269LysHis: 1.269 ± 0.045	3.572LysIle: 3.572 ± 0.064	4.817LysLys: 4.817 ± 0.105	5.205LysLeu: 5.205 ± 0.087	2.134LysMet: 2.134 ± 0.059	3.383LysAsn: 3.383 ± 0.075	2.316LysPro: 2.316 ± 0.061	2.74LysGln: 2.74 ± 0.059	3.396LysArg: 3.396 ± 0.069	3.421LysSer: 3.421 ± 0.073	3.82LysThr: 3.82 ± 0.066	4.301LysVal: 4.301 ± 0.083	0.785LysTrp: 0.785 ± 0.033	2.784LysTyr: 2.784 ± 0.062	0.0LysXaa: 0.0 ± 0.0
Leu	6.93LeuAla: 6.93 ± 0.106	1.343LeuCys: 1.343 ± 0.05	4.716LeuAsp: 4.716 ± 0.082	4.269LeuGlu: 4.269 ± 0.091	3.679LeuPhe: 3.679 ± 0.081	5.61LeuGly: 5.61 ± 0.114	1.85LeuHis: 1.85 ± 0.043	4.563LeuIle: 4.563 ± 0.08	5.672LeuLys: 5.672 ± 0.096	7.68LeuLeu: 7.68 ± 0.146	2.487LeuMet: 2.487 ± 0.058	4.412LeuAsn: 4.412 ± 0.09	3.633LeuPro: 3.633 ± 0.071	3.164LeuGln: 3.164 ± 0.07	4.635LeuArg: 4.635 ± 0.082	6.027LeuSer: 6.027 ± 0.099	5.291LeuThr: 5.291 ± 0.086	5.343LeuVal: 5.343 ± 0.107	0.923LeuTrp: 0.923 ± 0.039	3.245LeuTyr: 3.245 ± 0.078	0.0LeuXaa: 0.0 ± 0.0
Met	2.351MetAla: 2.351 ± 0.054	0.316MetCys: 0.316 ± 0.02	1.501MetAsp: 1.501 ± 0.045	1.715MetGlu: 1.715 ± 0.043	1.126MetPhe: 1.126 ± 0.042	1.881MetGly: 1.881 ± 0.054	0.592MetHis: 0.592 ± 0.029	1.473MetIle: 1.473 ± 0.043	2.398MetLys: 2.398 ± 0.052	2.898MetLeu: 2.898 ± 0.057	0.926MetMet: 0.926 ± 0.039	1.53MetAsn: 1.53 ± 0.052	1.387MetPro: 1.387 ± 0.046	1.283MetGln: 1.283 ± 0.04	1.544MetArg: 1.544 ± 0.051	1.741MetSer: 1.741 ± 0.047	1.653MetThr: 1.653 ± 0.051	1.702MetVal: 1.702 ± 0.051	0.262MetTrp: 0.262 ± 0.021	0.918MetTyr: 0.918 ± 0.036	0.0MetXaa: 0.0 ± 0.0
Asn	3.994AsnAla: 3.994 ± 0.068	0.627AsnCys: 0.627 ± 0.03	2.927AsnAsp: 2.927 ± 0.061	2.875AsnGlu: 2.875 ± 0.072	2.102AsnPhe: 2.102 ± 0.059	4.117AsnGly: 4.117 ± 0.093	0.932AsnHis: 0.932 ± 0.034	3.658AsnIle: 3.658 ± 0.081	2.894AsnLys: 2.894 ± 0.078	3.912AsnLeu: 3.912 ± 0.071	1.332AsnMet: 1.332 ± 0.045	2.768AsnAsn: 2.768 ± 0.077	2.252AsnPro: 2.252 ± 0.062	1.361AsnGln: 1.361 ± 0.046	2.462AsnArg: 2.462 ± 0.058	2.712AsnSer: 2.712 ± 0.07	2.877AsnThr: 2.877 ± 0.065	3.77AsnVal: 3.77 ± 0.078	0.667AsnTrp: 0.667 ± 0.035	2.172AsnTyr: 2.172 ± 0.06	0.0AsnXaa: 0.0 ± 0.0
Pro	2.809ProAla: 2.809 ± 0.059	0.387ProCys: 0.387 ± 0.026	2.366ProAsp: 2.366 ± 0.063	2.983ProGlu: 2.983 ± 0.063	1.699ProPhe: 1.699 ± 0.048	2.191ProGly: 2.191 ± 0.054	0.72ProHis: 0.72 ± 0.033	1.989ProIle: 1.989 ± 0.049	2.106ProLys: 2.106 ± 0.058	2.931ProLeu: 2.931 ± 0.069	1.044ProMet: 1.044 ± 0.039	1.709ProAsn: 1.709 ± 0.051	0.713ProPro: 0.713 ± 0.034	1.43ProGln: 1.43 ± 0.042	1.348ProArg: 1.348 ± 0.041	2.218ProSer: 2.218 ± 0.063	2.312ProThr: 2.312 ± 0.068	2.595ProVal: 2.595 ± 0.06	0.438ProTrp: 0.438 ± 0.027	1.588ProTyr: 1.588 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	2.277GlnAla: 2.277 ± 0.059	0.38GlnCys: 0.38 ± 0.021	1.466GlnAsp: 1.466 ± 0.044	1.818GlnGlu: 1.818 ± 0.058	1.365GlnPhe: 1.365 ± 0.037	1.887GlnGly: 1.887 ± 0.054	0.777GlnHis: 0.777 ± 0.036	2.104GlnIle: 2.104 ± 0.052	2.356GlnLys: 2.356 ± 0.066	3.534GlnLeu: 3.534 ± 0.075	1.222GlnMet: 1.222 ± 0.04	1.858GlnAsn: 1.858 ± 0.052	1.398GlnPro: 1.398 ± 0.047	1.78GlnGln: 1.78 ± 0.062	2.028GlnArg: 2.028 ± 0.051	1.867GlnSer: 1.867 ± 0.053	2.238GlnThr: 2.238 ± 0.055	1.948GlnVal: 1.948 ± 0.051	0.47GlnTrp: 0.47 ± 0.026	1.433GlnTyr: 1.433 ± 0.049	0.0GlnXaa: 0.0 ± 0.0
Arg	3.108ArgAla: 3.108 ± 0.072	0.552ArgCys: 0.552 ± 0.021	2.457ArgAsp: 2.457 ± 0.058	2.98ArgGlu: 2.98 ± 0.076	2.257ArgPhe: 2.257 ± 0.056	2.722ArgGly: 2.722 ± 0.062	1.122ArgHis: 1.122 ± 0.042	3.499ArgIle: 3.499 ± 0.07	3.25ArgLys: 3.25 ± 0.067	4.754ArgLeu: 4.754 ± 0.084	1.652ArgMet: 1.652 ± 0.046	2.47ArgAsn: 2.47 ± 0.059	1.693ArgPro: 1.693 ± 0.051	1.999ArgGln: 1.999 ± 0.055	2.843ArgArg: 2.843 ± 0.075	2.654ArgSer: 2.654 ± 0.059	2.662ArgThr: 2.662 ± 0.061	2.914ArgVal: 2.914 ± 0.069	0.618ArgTrp: 0.618 ± 0.026	2.261ArgTyr: 2.261 ± 0.06	0.001ArgXaa: 0.001 ± 0.001
Ser	4.989SerAla: 4.989 ± 0.084	0.853SerCys: 0.853 ± 0.034	3.534SerAsp: 3.534 ± 0.072	3.365SerGlu: 3.365 ± 0.074	2.859SerPhe: 2.859 ± 0.062	4.237SerGly: 4.237 ± 0.08	1.21SerHis: 1.21 ± 0.039	3.765SerIle: 3.765 ± 0.079	3.43SerLys: 3.43 ± 0.068	5.421SerLeu: 5.421 ± 0.092	1.61SerMet: 1.61 ± 0.044	2.72SerAsn: 2.72 ± 0.077	2.128SerPro: 2.128 ± 0.049	1.983SerGln: 1.983 ± 0.058	2.586SerArg: 2.586 ± 0.063	3.657SerSer: 3.657 ± 0.101	3.528SerThr: 3.528 ± 0.081	4.631SerVal: 4.631 ± 0.089	0.767SerTrp: 0.767 ± 0.035	2.581SerTyr: 2.581 ± 0.073	0.003SerXaa: 0.003 ± 0.002
Thr	4.986ThrAla: 4.986 ± 0.087	0.685ThrCys: 0.685 ± 0.032	3.79ThrAsp: 3.79 ± 0.075	3.275ThrGlu: 3.275 ± 0.071	2.715ThrPhe: 2.715 ± 0.06	4.053ThrGly: 4.053 ± 0.073	1.073ThrHis: 1.073 ± 0.036	4.035ThrIle: 4.035 ± 0.084	3.187ThrLys: 3.187 ± 0.067	5.735ThrLeu: 5.735 ± 0.092	1.496ThrMet: 1.496 ± 0.048	2.478ThrAsn: 2.478 ± 0.056	2.729ThrPro: 2.729 ± 0.067	1.825ThrGln: 1.825 ± 0.05	2.298ThrArg: 2.298 ± 0.049	3.548ThrSer: 3.548 ± 0.078	3.793ThrThr: 3.793 ± 0.086	4.209ThrVal: 4.209 ± 0.077	0.755ThrTrp: 0.755 ± 0.031	2.508ThrTyr: 2.508 ± 0.075	0.001ThrXaa: 0.001 ± 0.001
Val	5.731ValAla: 5.731 ± 0.102	1.165ValCys: 1.165 ± 0.04	4.364ValAsp: 4.364 ± 0.081	4.569ValGlu: 4.569 ± 0.084	2.895ValPhe: 2.895 ± 0.064	4.665ValGly: 4.665 ± 0.078	1.111ValHis: 1.111 ± 0.039	3.827ValIle: 3.827 ± 0.077	4.761ValLys: 4.761 ± 0.087	5.781ValLeu: 5.781 ± 0.092	1.878ValMet: 1.878 ± 0.062	3.385ValAsn: 3.385 ± 0.082	2.593ValPro: 2.593 ± 0.061	2.045ValGln: 2.045 ± 0.053	3.345ValArg: 3.345 ± 0.057	4.626ValSer: 4.626 ± 0.085	3.886ValThr: 3.886 ± 0.085	5.534ValVal: 5.534 ± 0.106	0.795ValTrp: 0.795 ± 0.032	2.832ValTyr: 2.832 ± 0.069	0.0ValXaa: 0.0 ± 0.0
Trp	0.83TrpAla: 0.83 ± 0.037	0.183TrpCys: 0.183 ± 0.017	0.686TrpAsp: 0.686 ± 0.031	0.608TrpGlu: 0.608 ± 0.032	0.498TrpPhe: 0.498 ± 0.026	0.909TrpGly: 0.909 ± 0.038	0.332TrpHis: 0.332 ± 0.023	0.676TrpIle: 0.676 ± 0.028	0.748TrpLys: 0.748 ± 0.034	1.172TrpLeu: 1.172 ± 0.041	0.399TrpMet: 0.399 ± 0.024	0.72TrpAsn: 0.72 ± 0.034	0.254TrpPro: 0.254 ± 0.019	0.597TrpGln: 0.597 ± 0.028	0.642TrpArg: 0.642 ± 0.033	0.723TrpSer: 0.723 ± 0.028	0.777TrpThr: 0.777 ± 0.04	0.712TrpVal: 0.712 ± 0.032	0.152TrpTrp: 0.152 ± 0.016	0.448TrpTyr: 0.448 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.273TyrAla: 3.273 ± 0.064	0.603TyrCys: 0.603 ± 0.031	2.839TyrAsp: 2.839 ± 0.061	2.441TyrGlu: 2.441 ± 0.056	1.828TyrPhe: 1.828 ± 0.048	2.873TyrGly: 2.873 ± 0.071	0.757TyrHis: 0.757 ± 0.034	2.586TyrIle: 2.586 ± 0.057	2.572TyrLys: 2.572 ± 0.057	3.136TyrLeu: 3.136 ± 0.077	1.15TyrMet: 1.15 ± 0.037	2.438TyrAsn: 2.438 ± 0.063	1.572TyrPro: 1.572 ± 0.048	1.236TyrGln: 1.236 ± 0.041	2.192TyrArg: 2.192 ± 0.063	2.657TyrSer: 2.657 ± 0.068	2.698TyrThr: 2.698 ± 0.078	2.896TyrVal: 2.896 ± 0.062	0.534TyrTrp: 0.534 ± 0.026	2.051TyrTyr: 2.051 ± 0.065	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.003XaaAla: 0.003 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.003XaaPro: 0.003 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 2171 proteins (780981 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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