Myotis ricketti papillomavirus 1
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I3VR47|I3VR47_9PAPI Regulatory protein E2 OS=Myotis ricketti papillomavirus 1 OX=1195370 GN=E2 PE=3 SV=1
MM1 pKa = 7.75 ANPPGNNGGTGCIYY15 pKa = 10.87 LLDD18 pKa = 4.2 EE19 pKa = 5.06 AEE21 pKa = 4.74 CDD23 pKa = 4.14 DD24 pKa = 4.4 GSSDD28 pKa = 3.49 EE29 pKa = 4.94 DD30 pKa = 3.75 EE31 pKa = 4.72 EE32 pKa = 4.45 EE33 pKa = 4.46 EE34 pKa = 5.06 EE35 pKa = 4.38 VGEE38 pKa = 4.93 DD39 pKa = 3.86 CVDD42 pKa = 4.81 DD43 pKa = 4.12 AACYY47 pKa = 9.56 QGNTIALFVAQEE59 pKa = 3.84 ASTDD63 pKa = 3.52 EE64 pKa = 5.77 AIIQSLKK71 pKa = 10.15 RR72 pKa = 11.84 KK73 pKa = 8.33 YY74 pKa = 10.83 VKK76 pKa = 9.83 TPQQDD81 pKa = 3.47 RR82 pKa = 11.84 IRR84 pKa = 11.84 EE85 pKa = 4.1 IEE87 pKa = 4.06 EE88 pKa = 4.21 EE89 pKa = 3.65 ISPRR93 pKa = 11.84 LEE95 pKa = 4.6 GIHH98 pKa = 7.0 LEE100 pKa = 4.19 THH102 pKa = 4.5 SQKK105 pKa = 10.41 VKK107 pKa = 10.72 RR108 pKa = 11.84 KK109 pKa = 9.71 LFQANNAAKK118 pKa = 10.71 GEE120 pKa = 4.16 LDD122 pKa = 3.51 VPLEE126 pKa = 4.3 KK127 pKa = 10.44 KK128 pKa = 10.31 VATAGNLVEE137 pKa = 4.99 VVAEE141 pKa = 4.33 VHH143 pKa = 6.31 CPPAGHH149 pKa = 7.46 DD150 pKa = 3.5 EE151 pKa = 4.62 TVCSQKK157 pKa = 11.16 SMEE160 pKa = 4.28 GVEE163 pKa = 4.24 EE164 pKa = 3.89 QDD166 pKa = 4.13 EE167 pKa = 5.09 LGTCDD172 pKa = 3.15 TGQEE176 pKa = 4.15 AEE178 pKa = 4.29 SALQNTEE185 pKa = 4.03 PEE187 pKa = 4.28 IEE189 pKa = 4.3 CLSLEE194 pKa = 3.98 ITEE197 pKa = 4.66 PPVIPEE203 pKa = 4.19 RR204 pKa = 11.84 PWLDD208 pKa = 3.0 GCTTEE213 pKa = 4.65 QEE215 pKa = 3.88 IAAAHH220 pKa = 6.15 AKK222 pKa = 9.5 AKK224 pKa = 10.38 KK225 pKa = 9.45 IAALKK230 pKa = 9.65 LQEE233 pKa = 4.22 KK234 pKa = 10.37 AKK236 pKa = 11.04 LPIQLLKK243 pKa = 9.86 TGNRR247 pKa = 11.84 KK248 pKa = 8.64 AQILSHH254 pKa = 6.1 CKK256 pKa = 8.48 KK257 pKa = 8.91 TFGISFIDD265 pKa = 3.11 ITRR268 pKa = 11.84 PFKK271 pKa = 11.0 NDD273 pKa = 2.74 NTVNEE278 pKa = 4.26 TWVILSLYY286 pKa = 10.32 LEE288 pKa = 4.58 EE289 pKa = 4.52 EE290 pKa = 4.34 VADD293 pKa = 4.07 ALLTVLEE300 pKa = 4.65 PHH302 pKa = 6.91 CKK304 pKa = 9.69 CCFIISKK311 pKa = 10.27 YY312 pKa = 10.53 FGILKK317 pKa = 10.27 SVLILIEE324 pKa = 4.03 FQTAKK329 pKa = 10.32 SRR331 pKa = 11.84 KK332 pKa = 6.91 TVLKK336 pKa = 10.81 LMSSVMAVDD345 pKa = 4.17 PEE347 pKa = 4.74 AIIADD352 pKa = 3.97 PPRR355 pKa = 11.84 VRR357 pKa = 11.84 SMPAALYY364 pKa = 7.98 WYY366 pKa = 10.23 KK367 pKa = 11.08 VIAFEE372 pKa = 4.81 KK373 pKa = 9.61 AAQFGEE379 pKa = 4.13 IPGWILTQVTLQHH392 pKa = 6.48 SEE394 pKa = 3.96 EE395 pKa = 4.52 SEE397 pKa = 4.38 LPFDD401 pKa = 4.98 LSVMVQWAYY410 pKa = 11.31 DD411 pKa = 3.45 NDD413 pKa = 3.85 YY414 pKa = 9.69 DD415 pKa = 4.02 TDD417 pKa = 3.88 YY418 pKa = 11.47 DD419 pKa = 4.03 IAFEE423 pKa = 4.09 YY424 pKa = 10.9 AKK426 pKa = 10.75 LATADD431 pKa = 3.57 RR432 pKa = 11.84 NARR435 pKa = 11.84 AFLNSNCQAKK445 pKa = 9.54 YY446 pKa = 11.01 VKK448 pKa = 10.23 DD449 pKa = 3.8 CQTMVRR455 pKa = 11.84 LYY457 pKa = 10.67 RR458 pKa = 11.84 KK459 pKa = 9.58 AQMHH463 pKa = 5.98 RR464 pKa = 11.84 MSMGAWIKK472 pKa = 10.37 EE473 pKa = 3.96 RR474 pKa = 11.84 SDD476 pKa = 4.72 KK477 pKa = 10.78 IDD479 pKa = 3.6 GDD481 pKa = 4.02 GDD483 pKa = 3.23 WRR485 pKa = 11.84 IIVHH489 pKa = 5.88 FLRR492 pKa = 11.84 YY493 pKa = 9.59 QEE495 pKa = 4.29 VEE497 pKa = 4.21 FVSFMVKK504 pKa = 10.46 FKK506 pKa = 11.11 LFLKK510 pKa = 7.95 GTPKK514 pKa = 10.42 KK515 pKa = 10.59 NCLLFYY521 pKa = 10.61 GPPNTGKK528 pKa = 10.3 SAFCMSLIKK537 pKa = 10.37 FFAGRR542 pKa = 11.84 VVSFMNARR550 pKa = 11.84 SHH552 pKa = 7.32 FWLQPLADD560 pKa = 4.26 CRR562 pKa = 11.84 VGLLDD567 pKa = 5.71 DD568 pKa = 4.59 ATDD571 pKa = 3.8 ACWDD575 pKa = 3.74 YY576 pKa = 11.46 VDD578 pKa = 4.08 TYY580 pKa = 10.47 MRR582 pKa = 11.84 SAFDD586 pKa = 3.57 GNEE589 pKa = 3.25 ISLDD593 pKa = 4.03 CKK595 pKa = 10.61 HH596 pKa = 6.61 KK597 pKa = 11.14 APIQLKK603 pKa = 10.0 CPPLLVTSNTNILTSDD619 pKa = 2.78 RR620 pKa = 11.84 WKK622 pKa = 10.15 YY623 pKa = 8.81 LHH625 pKa = 7.03 SRR627 pKa = 11.84 VTVVKK632 pKa = 9.9 FMNPFPLTQDD642 pKa = 3.07 GQPRR646 pKa = 11.84 YY647 pKa = 10.38 NFQPKK652 pKa = 9.24 DD653 pKa = 3.17 WKK655 pKa = 11.13 SFLSRR660 pKa = 11.84 LWARR664 pKa = 11.84 LDD666 pKa = 3.47 LSEE669 pKa = 4.9 PEE671 pKa = 4.16 EE672 pKa = 4.51 DD673 pKa = 5.4 EE674 pKa = 4.62 EE675 pKa = 5.38 DD676 pKa = 3.13 EE677 pKa = 5.0 GYY679 pKa = 11.16 NGDD682 pKa = 3.57 SQEE685 pKa = 4.43 KK686 pKa = 8.63 FRR688 pKa = 11.84 CTIRR692 pKa = 11.84 EE693 pKa = 3.96 NDD695 pKa = 3.13 EE696 pKa = 4.07 HH697 pKa = 7.89 LL698 pKa = 4.27
Molecular weight: 79.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.913
IPC2_protein 4.952
IPC_protein 4.914
Toseland 4.8
ProMoST 5.029
Dawson 4.876
Bjellqvist 5.016
Wikipedia 4.724
Rodwell 4.787
Grimsley 4.724
Solomon 4.876
Lehninger 4.825
Nozaki 4.978
DTASelect 5.13
Thurlkill 4.8
EMBOSS 4.762
Sillero 5.054
Patrickios 3.923
IPC_peptide 4.876
IPC2_peptide 5.054
IPC2.peptide.svr19 4.993
Protein with the highest isoelectric point:
>tr|I3VR46|I3VR46_9PAPI Replication protein E1 OS=Myotis ricketti papillomavirus 1 OX=1195370 GN=E1 PE=3 SV=1
MM1 pKa = 7.27 MIVVNYY7 pKa = 9.4 YY8 pKa = 10.34 DD9 pKa = 3.44 KK10 pKa = 10.97 KK11 pKa = 10.68 GKK13 pKa = 9.28 FRR15 pKa = 11.84 RR16 pKa = 11.84 DD17 pKa = 3.13 RR18 pKa = 11.84 SRR20 pKa = 11.84 SQYY23 pKa = 10.64 ISIRR27 pKa = 11.84 PIRR30 pKa = 11.84 GASMGSVTKK39 pKa = 10.76 RR40 pKa = 11.84 EE41 pKa = 4.15 DD42 pKa = 3.13 GEE44 pKa = 4.04 YY45 pKa = 10.16 RR46 pKa = 11.84 PRR48 pKa = 11.84 SLRR51 pKa = 11.84 EE52 pKa = 3.62 LMVDD56 pKa = 3.95 MGLQYY61 pKa = 9.49 QTILLSCVYY70 pKa = 10.04 CSKK73 pKa = 9.72 TLEE76 pKa = 4.03 VSEE79 pKa = 5.0 LVTFSVKK86 pKa = 10.31 DD87 pKa = 3.39 LQIIWRR93 pKa = 11.84 GGFPYY98 pKa = 10.25 GVCAFCLEE106 pKa = 4.46 FNLQIQYY113 pKa = 10.19 LRR115 pKa = 11.84 HH116 pKa = 5.75 HH117 pKa = 6.81 RR118 pKa = 11.84 RR119 pKa = 11.84 SASIKK124 pKa = 8.35 TVEE127 pKa = 4.58 EE128 pKa = 3.83 EE129 pKa = 4.0 LQRR132 pKa = 11.84 PVGNLNLRR140 pKa = 11.84 CFVCAIKK147 pKa = 10.55 LHH149 pKa = 5.45 STDD152 pKa = 3.41 YY153 pKa = 10.58 ATMEE157 pKa = 4.05 EE158 pKa = 3.93 QGLNLKK164 pKa = 10.09 YY165 pKa = 10.25 IAGQWRR171 pKa = 11.84 GKK173 pKa = 10.83 CMICIGAVEE182 pKa = 4.43 GVV184 pKa = 3.21
Molecular weight: 21.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.615
IPC2_protein 8.463
IPC_protein 8.39
Toseland 8.492
ProMoST 8.829
Dawson 9.077
Bjellqvist 9.238
Wikipedia 9.151
Rodwell 9.136
Grimsley 8.551
Solomon 9.194
Lehninger 9.18
Nozaki 9.37
DTASelect 9.019
Thurlkill 9.077
EMBOSS 9.224
Sillero 9.341
Patrickios 4.24
IPC_peptide 9.194
IPC2_peptide 8.375
IPC2.peptide.svr19 8.052
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2174
184
698
434.8
48.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.808 ± 0.558
2.162 ± 0.48
5.888 ± 0.448
6.348 ± 0.917
4.232 ± 0.492
6.118 ± 0.643
2.024 ± 0.099
5.152 ± 0.482
5.566 ± 0.629
8.05 ± 0.331
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.07 ± 0.32
3.588 ± 0.416
6.348 ± 0.861
4.324 ± 0.316
5.888 ± 0.582
7.498 ± 0.738
6.946 ± 0.787
5.934 ± 0.261
1.334 ± 0.321
3.726 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here