Amino acid dipepetide frequency for Rothia nasimurium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.024AlaAla: 15.024 ± 0.2
0.704AlaCys: 0.704 ± 0.03
6.538AlaAsp: 6.538 ± 0.095
8.105AlaGlu: 8.105 ± 0.112
3.562AlaPhe: 3.562 ± 0.082
10.526AlaGly: 10.526 ± 0.143
2.555AlaHis: 2.555 ± 0.061
5.099AlaIle: 5.099 ± 0.092
3.358AlaLys: 3.358 ± 0.076
13.308AlaLeu: 13.308 ± 0.19
2.395AlaMet: 2.395 ± 0.067
2.731AlaAsn: 2.731 ± 0.066
5.588AlaPro: 5.588 ± 0.1
4.894AlaGln: 4.894 ± 0.088
7.102AlaArg: 7.102 ± 0.12
7.065AlaSer: 7.065 ± 0.11
7.46AlaThr: 7.46 ± 0.114
9.016AlaVal: 9.016 ± 0.128
1.472AlaTrp: 1.472 ± 0.048
2.648AlaTyr: 2.648 ± 0.062
0.0AlaXaa: 0.0 ± 0.0
Cys
0.748CysAla: 0.748 ± 0.032
0.059CysCys: 0.059 ± 0.01
0.311CysAsp: 0.311 ± 0.024
0.322CysGlu: 0.322 ± 0.024
0.206CysPhe: 0.206 ± 0.018
0.655CysGly: 0.655 ± 0.032
0.141CysHis: 0.141 ± 0.014
0.295CysIle: 0.295 ± 0.021
0.131CysLys: 0.131 ± 0.012
0.62CysLeu: 0.62 ± 0.031
0.118CysMet: 0.118 ± 0.011
0.136CysAsn: 0.136 ± 0.014
0.344CysPro: 0.344 ± 0.022
0.19CysGln: 0.19 ± 0.017
0.249CysArg: 0.249 ± 0.017
0.431CysSer: 0.431 ± 0.026
0.408CysThr: 0.408 ± 0.025
0.394CysVal: 0.394 ± 0.025
0.069CysTrp: 0.069 ± 0.01
0.16CysTyr: 0.16 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
6.21AspAla: 6.21 ± 0.105
0.307AspCys: 0.307 ± 0.022
2.604AspAsp: 2.604 ± 0.06
3.784AspGlu: 3.784 ± 0.078
2.122AspPhe: 2.122 ± 0.049
4.31AspGly: 4.31 ± 0.071
1.066AspHis: 1.066 ± 0.044
2.85AspIle: 2.85 ± 0.072
1.406AspLys: 1.406 ± 0.046
5.355AspLeu: 5.355 ± 0.088
1.056AspMet: 1.056 ± 0.039
1.138AspAsn: 1.138 ± 0.049
3.048AspPro: 3.048 ± 0.062
1.79AspGln: 1.79 ± 0.052
3.21AspArg: 3.21 ± 0.067
3.118AspSer: 3.118 ± 0.068
3.05AspThr: 3.05 ± 0.07
3.901AspVal: 3.901 ± 0.074
0.708AspTrp: 0.708 ± 0.031
1.794AspTyr: 1.794 ± 0.051
0.0AspXaa: 0.0 ± 0.0
Glu
7.618GluAla: 7.618 ± 0.125
0.284GluCys: 0.284 ± 0.02
3.138GluAsp: 3.138 ± 0.072
4.053GluGlu: 4.053 ± 0.097
1.807GluPhe: 1.807 ± 0.048
4.729GluGly: 4.729 ± 0.085
1.582GluHis: 1.582 ± 0.048
3.213GluIle: 3.213 ± 0.068
2.756GluLys: 2.756 ± 0.071
6.581GluLeu: 6.581 ± 0.111
1.193GluMet: 1.193 ± 0.039
2.329GluAsn: 2.329 ± 0.064
2.854GluPro: 2.854 ± 0.068
2.995GluGln: 2.995 ± 0.07
4.229GluArg: 4.229 ± 0.08
3.27GluSer: 3.27 ± 0.061
3.696GluThr: 3.696 ± 0.076
4.6GluVal: 4.6 ± 0.091
0.686GluTrp: 0.686 ± 0.031
1.406GluTyr: 1.406 ± 0.047
0.0GluXaa: 0.0 ± 0.0
Phe
3.847PheAla: 3.847 ± 0.083
0.295PheCys: 0.295 ± 0.022
2.215PheAsp: 2.215 ± 0.054
2.227PheGlu: 2.227 ± 0.061
1.339PhePhe: 1.339 ± 0.052
3.189PheGly: 3.189 ± 0.078
0.544PheHis: 0.544 ± 0.029
1.885PheIle: 1.885 ± 0.054
1.009PheLys: 1.009 ± 0.041
2.859PheLeu: 2.859 ± 0.067
0.797PheMet: 0.797 ± 0.035
1.098PheAsn: 1.098 ± 0.037
1.344PhePro: 1.344 ± 0.038
0.737PheGln: 0.737 ± 0.03
1.441PheArg: 1.441 ± 0.043
2.435PheSer: 2.435 ± 0.06
2.656PheThr: 2.656 ± 0.066
2.487PheVal: 2.487 ± 0.063
0.573PheTrp: 0.573 ± 0.029
0.947PheTyr: 0.947 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
9.306GlyAla: 9.306 ± 0.146
0.531GlyCys: 0.531 ± 0.028
3.897GlyAsp: 3.897 ± 0.078
5.007GlyGlu: 5.007 ± 0.089
3.387GlyPhe: 3.387 ± 0.075
6.777GlyGly: 6.777 ± 0.138
1.755GlyHis: 1.755 ± 0.054
4.623GlyIle: 4.623 ± 0.087
3.138GlyLys: 3.138 ± 0.073
8.405GlyLeu: 8.405 ± 0.119
1.865GlyMet: 1.865 ± 0.056
2.23GlyAsn: 2.23 ± 0.052
3.017GlyPro: 3.017 ± 0.068
3.357GlyGln: 3.357 ± 0.071
4.696GlyArg: 4.696 ± 0.077
5.522GlySer: 5.522 ± 0.091
5.27GlyThr: 5.27 ± 0.088
7.048GlyVal: 7.048 ± 0.09
1.328GlyTrp: 1.328 ± 0.04
2.593GlyTyr: 2.593 ± 0.065
0.0GlyXaa: 0.0 ± 0.0
His
1.993HisAla: 1.993 ± 0.054
0.149HisCys: 0.149 ± 0.015
1.059HisAsp: 1.059 ± 0.043
1.27HisGlu: 1.27 ± 0.048
0.665HisPhe: 0.665 ± 0.029
1.651HisGly: 1.651 ± 0.056
0.597HisHis: 0.597 ± 0.034
1.055HisIle: 1.055 ± 0.035
0.564HisLys: 0.564 ± 0.03
2.367HisLeu: 2.367 ± 0.061
0.444HisMet: 0.444 ± 0.025
0.573HisAsn: 0.573 ± 0.027
1.319HisPro: 1.319 ± 0.047
0.776HisGln: 0.776 ± 0.031
1.551HisArg: 1.551 ± 0.052
1.218HisSer: 1.218 ± 0.042
1.343HisThr: 1.343 ± 0.049
1.327HisVal: 1.327 ± 0.047
0.32HisTrp: 0.32 ± 0.023
0.577HisTyr: 0.577 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.067IleAla: 6.067 ± 0.102
0.397IleCys: 0.397 ± 0.023
3.294IleAsp: 3.294 ± 0.069
3.337IleGlu: 3.337 ± 0.075
1.956IlePhe: 1.956 ± 0.052
4.189IleGly: 4.189 ± 0.08
0.861IleHis: 0.861 ± 0.034
2.62IleIle: 2.62 ± 0.063
1.584IleLys: 1.584 ± 0.049
4.147IleLeu: 4.147 ± 0.091
1.019IleMet: 1.019 ± 0.042
1.658IleAsn: 1.658 ± 0.045
2.466IlePro: 2.466 ± 0.049
1.331IleGln: 1.331 ± 0.046
2.478IleArg: 2.478 ± 0.056
3.278IleSer: 3.278 ± 0.072
3.431IleThr: 3.431 ± 0.067
3.75IleVal: 3.75 ± 0.082
0.632IleTrp: 0.632 ± 0.034
1.282IleTyr: 1.282 ± 0.042
0.0IleXaa: 0.0 ± 0.0
Lys
3.618LysAla: 3.618 ± 0.078
0.11LysCys: 0.11 ± 0.012
1.87LysAsp: 1.87 ± 0.053
1.869LysGlu: 1.869 ± 0.048
0.966LysPhe: 0.966 ± 0.037
2.135LysGly: 2.135 ± 0.064
0.678LysHis: 0.678 ± 0.03
1.943LysIle: 1.943 ± 0.061
1.763LysLys: 1.763 ± 0.078
2.917LysLeu: 2.917 ± 0.062
0.741LysMet: 0.741 ± 0.031
1.319LysAsn: 1.319 ± 0.041
1.681LysPro: 1.681 ± 0.055
1.148LysGln: 1.148 ± 0.04
1.806LysArg: 1.806 ± 0.05
1.721LysSer: 1.721 ± 0.059
2.133LysThr: 2.133 ± 0.056
2.569LysVal: 2.569 ± 0.068
0.288LysTrp: 0.288 ± 0.02
0.845LysTyr: 0.845 ± 0.036
0.0LysXaa: 0.0 ± 0.0
Leu
13.586LeuAla: 13.586 ± 0.188
0.576LeuCys: 0.576 ± 0.033
5.49LeuAsp: 5.49 ± 0.087
5.749LeuGlu: 5.749 ± 0.094
3.091LeuPhe: 3.091 ± 0.073
8.964LeuGly: 8.964 ± 0.125
1.921LeuHis: 1.921 ± 0.057
5.105LeuIle: 5.105 ± 0.112
3.096LeuLys: 3.096 ± 0.07
10.375LeuLeu: 10.375 ± 0.182
2.211LeuMet: 2.211 ± 0.059
3.023LeuAsn: 3.023 ± 0.066
5.613LeuPro: 5.613 ± 0.103
2.591LeuGln: 2.591 ± 0.062
5.659LeuArg: 5.659 ± 0.093
5.896LeuSer: 5.896 ± 0.095
7.577LeuThr: 7.577 ± 0.115
8.436LeuVal: 8.436 ± 0.13
1.231LeuTrp: 1.231 ± 0.04
2.192LeuTyr: 2.192 ± 0.057
0.0LeuXaa: 0.0 ± 0.0
Met
2.387MetAla: 2.387 ± 0.065
0.118MetCys: 0.118 ± 0.013
0.965MetAsp: 0.965 ± 0.033
0.884MetGlu: 0.884 ± 0.039
0.677MetPhe: 0.677 ± 0.031
1.8MetGly: 1.8 ± 0.05
0.371MetHis: 0.371 ± 0.026
1.074MetIle: 1.074 ± 0.039
0.753MetLys: 0.753 ± 0.031
2.048MetLeu: 2.048 ± 0.057
0.519MetMet: 0.519 ± 0.027
0.725MetAsn: 0.725 ± 0.03
1.074MetPro: 1.074 ± 0.039
0.689MetGln: 0.689 ± 0.03
1.242MetArg: 1.242 ± 0.043
1.554MetSer: 1.554 ± 0.048
1.679MetThr: 1.679 ± 0.043
1.721MetVal: 1.721 ± 0.045
0.237MetTrp: 0.237 ± 0.02
0.428MetTyr: 0.428 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
2.665AsnAla: 2.665 ± 0.069
0.157AsnCys: 0.157 ± 0.014
1.317AsnAsp: 1.317 ± 0.039
1.561AsnGlu: 1.561 ± 0.048
1.049AsnPhe: 1.049 ± 0.039
2.211AsnGly: 2.211 ± 0.058
0.642AsnHis: 0.642 ± 0.031
1.597AsnIle: 1.597 ± 0.047
0.857AsnLys: 0.857 ± 0.036
3.08AsnLeu: 3.08 ± 0.066
0.591AsnMet: 0.591 ± 0.032
0.885AsnAsn: 0.885 ± 0.039
2.153AsnPro: 2.153 ± 0.054
1.119AsnGln: 1.119 ± 0.044
1.81AsnArg: 1.81 ± 0.05
1.737AsnSer: 1.737 ± 0.06
1.81AsnThr: 1.81 ± 0.063
1.975AsnVal: 1.975 ± 0.048
0.459AsnTrp: 0.459 ± 0.026
0.907AsnTyr: 0.907 ± 0.04
0.0AsnXaa: 0.0 ± 0.0
Pro
6.433ProAla: 6.433 ± 0.122
0.227ProCys: 0.227 ± 0.018
2.687ProAsp: 2.687 ± 0.061
3.89ProGlu: 3.89 ± 0.073
1.539ProPhe: 1.539 ± 0.052
4.384ProGly: 4.384 ± 0.085
1.239ProHis: 1.239 ± 0.047
1.97ProIle: 1.97 ± 0.051
1.363ProLys: 1.363 ± 0.042
4.941ProLeu: 4.941 ± 0.098
0.801ProMet: 0.801 ± 0.036
1.304ProAsn: 1.304 ± 0.043
1.531ProPro: 1.531 ± 0.049
1.83ProGln: 1.83 ± 0.049
2.371ProArg: 2.371 ± 0.06
2.952ProSer: 2.952 ± 0.066
3.294ProThr: 3.294 ± 0.073
4.404ProVal: 4.404 ± 0.086
0.658ProTrp: 0.658 ± 0.027
1.224ProTyr: 1.224 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
4.557GlnAla: 4.557 ± 0.105
0.121GlnCys: 0.121 ± 0.015
1.592GlnAsp: 1.592 ± 0.052
2.086GlnGlu: 2.086 ± 0.068
0.893GlnPhe: 0.893 ± 0.032
2.731GlnGly: 2.731 ± 0.059
0.743GlnHis: 0.743 ± 0.03
1.724GlnIle: 1.724 ± 0.046
1.38GlnLys: 1.38 ± 0.044
4.105GlnLeu: 4.105 ± 0.088
0.83GlnMet: 0.83 ± 0.034
1.118GlnAsn: 1.118 ± 0.039
1.675GlnPro: 1.675 ± 0.049
1.769GlnGln: 1.769 ± 0.065
2.271GlnArg: 2.271 ± 0.054
1.568GlnSer: 1.568 ± 0.052
1.938GlnThr: 1.938 ± 0.049
3.305GlnVal: 3.305 ± 0.084
0.409GlnTrp: 0.409 ± 0.023
0.713GlnTyr: 0.713 ± 0.039
0.001GlnXaa: 0.001 ± 0.001
Arg
6.741ArgAla: 6.741 ± 0.103
0.335ArgCys: 0.335 ± 0.024
2.906ArgAsp: 2.906 ± 0.06
3.953ArgGlu: 3.953 ± 0.074
2.145ArgPhe: 2.145 ± 0.053
4.284ArgGly: 4.284 ± 0.075
1.124ArgHis: 1.124 ± 0.042
2.892ArgIle: 2.892 ± 0.062
1.954ArgLys: 1.954 ± 0.057
5.728ArgLeu: 5.728 ± 0.089
1.331ArgMet: 1.331 ± 0.039
1.557ArgAsn: 1.557 ± 0.043
2.749ArgPro: 2.749 ± 0.071
2.235ArgGln: 2.235 ± 0.055
3.925ArgArg: 3.925 ± 0.071
3.498ArgSer: 3.498 ± 0.066
3.683ArgThr: 3.683 ± 0.074
4.638ArgVal: 4.638 ± 0.085
0.951ArgTrp: 0.951 ± 0.034
1.677ArgTyr: 1.677 ± 0.046
0.001ArgXaa: 0.001 ± 0.001
Ser
7.049SerAla: 7.049 ± 0.105
0.374SerCys: 0.374 ± 0.026
2.96SerAsp: 2.96 ± 0.061
3.574SerGlu: 3.574 ± 0.084
2.168SerPhe: 2.168 ± 0.052
5.511SerGly: 5.511 ± 0.095
1.228SerHis: 1.228 ± 0.042
2.813SerIle: 2.813 ± 0.068
1.807SerLys: 1.807 ± 0.05
6.097SerLeu: 6.097 ± 0.104
1.475SerMet: 1.475 ± 0.045
1.577SerAsn: 1.577 ± 0.048
3.111SerPro: 3.111 ± 0.07
2.274SerGln: 2.274 ± 0.056
3.334SerArg: 3.334 ± 0.071
4.151SerSer: 4.151 ± 0.1
3.977SerThr: 3.977 ± 0.08
4.557SerVal: 4.557 ± 0.083
0.889SerTrp: 0.889 ± 0.029
1.654SerTyr: 1.654 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
7.48ThrAla: 7.48 ± 0.112
0.417ThrCys: 0.417 ± 0.026
3.588ThrAsp: 3.588 ± 0.079
3.998ThrGlu: 3.998 ± 0.073
2.073ThrPhe: 2.073 ± 0.055
6.119ThrGly: 6.119 ± 0.085
1.395ThrHis: 1.395 ± 0.048
2.963ThrIle: 2.963 ± 0.065
1.685ThrLys: 1.685 ± 0.058
6.636ThrLeu: 6.636 ± 0.107
1.187ThrMet: 1.187 ± 0.045
1.607ThrAsn: 1.607 ± 0.051
3.784ThrPro: 3.784 ± 0.073
2.016ThrGln: 2.016 ± 0.052
3.49ThrArg: 3.49 ± 0.075
3.945ThrSer: 3.945 ± 0.077
3.84ThrThr: 3.84 ± 0.078
6.028ThrVal: 6.028 ± 0.102
0.962ThrTrp: 0.962 ± 0.039
1.839ThrTyr: 1.839 ± 0.061
0.0ThrXaa: 0.0 ± 0.0
Val
9.619ValAla: 9.619 ± 0.127
0.527ValCys: 0.527 ± 0.026
4.359ValAsp: 4.359 ± 0.079
4.995ValGlu: 4.995 ± 0.086
2.719ValPhe: 2.719 ± 0.063
6.282ValGly: 6.282 ± 0.103
1.611ValHis: 1.611 ± 0.054
4.276ValIle: 4.276 ± 0.078
2.281ValLys: 2.281 ± 0.056
8.442ValLeu: 8.442 ± 0.115
1.643ValMet: 1.643 ± 0.052
2.353ValAsn: 2.353 ± 0.059
3.895ValPro: 3.895 ± 0.076
2.364ValGln: 2.364 ± 0.057
4.802ValArg: 4.802 ± 0.084
4.824ValSer: 4.824 ± 0.085
5.541ValThr: 5.541 ± 0.101
6.177ValVal: 6.177 ± 0.099
0.814ValTrp: 0.814 ± 0.037
1.741ValTyr: 1.741 ± 0.047
0.0ValXaa: 0.0 ± 0.0
Trp
1.572TrpAla: 1.572 ± 0.052
0.11TrpCys: 0.11 ± 0.014
0.653TrpAsp: 0.653 ± 0.028
0.751TrpGlu: 0.751 ± 0.033
0.558TrpPhe: 0.558 ± 0.028
1.101TrpGly: 1.101 ± 0.04
0.268TrpHis: 0.268 ± 0.022
0.654TrpIle: 0.654 ± 0.032
0.409TrpLys: 0.409 ± 0.024
1.454TrpLeu: 1.454 ± 0.045
0.277TrpMet: 0.277 ± 0.021
0.488TrpAsn: 0.488 ± 0.027
0.459TrpPro: 0.459 ± 0.022
0.55TrpGln: 0.55 ± 0.031
0.803TrpArg: 0.803 ± 0.029
0.797TrpSer: 0.797 ± 0.033
0.584TrpThr: 0.584 ± 0.026
1.242TrpVal: 1.242 ± 0.05
0.261TrpTrp: 0.261 ± 0.019
0.322TrpTyr: 0.322 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.632TyrAla: 2.632 ± 0.061
0.186TyrCys: 0.186 ± 0.019
1.38TyrAsp: 1.38 ± 0.054
1.608TyrGlu: 1.608 ± 0.046
1.001TyrPhe: 1.001 ± 0.037
2.201TyrGly: 2.201 ± 0.055
0.498TyrHis: 0.498 ± 0.027
1.126TyrIle: 1.126 ± 0.037
0.825TyrLys: 0.825 ± 0.036
2.917TyrLeu: 2.917 ± 0.06
0.451TyrMet: 0.451 ± 0.027
0.733TyrAsn: 0.733 ± 0.041
1.337TyrPro: 1.337 ± 0.049
0.875TyrGln: 0.875 ± 0.037
1.835TyrArg: 1.835 ± 0.049
1.603TyrSer: 1.603 ± 0.044
1.683TyrThr: 1.683 ± 0.06
1.691TyrVal: 1.691 ± 0.053
0.365TyrTrp: 0.365 ± 0.023
0.686TyrTyr: 0.686 ± 0.034
0.001TyrXaa: 0.001 ± 0.001
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.001XaaAsp: 0.001 ± 0.001
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.001XaaLys: 0.001 ± 0.001
0.0XaaLeu: 0.0 ± 0.0
0.001XaaMet: 0.001 ± 0.001
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2215 proteins (743213 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski