Acetobacter sp. DmW_043
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2062 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y3G1X4|A0A1Y3G1X4_9PROT Copper resistance protein CopB OS=Acetobacter sp. DmW_043 OX=1670658 GN=HK11_05965 PE=4 SV=1
MM1 pKa = 7.37 TYY3 pKa = 10.71 VVTEE7 pKa = 3.55 NCIRR11 pKa = 11.84 CKK13 pKa = 10.72 FMDD16 pKa = 4.33 CVEE19 pKa = 4.27 VCPVDD24 pKa = 3.86 CFYY27 pKa = 11.41 AGEE30 pKa = 4.18 NFLVINPDD38 pKa = 2.99 EE39 pKa = 5.52 CIDD42 pKa = 4.08 CGVCEE47 pKa = 4.44 PEE49 pKa = 4.91 CPAEE53 pKa = 4.95 AIFPDD58 pKa = 3.6 SDD60 pKa = 3.69 DD61 pKa = 4.62 RR62 pKa = 11.84 ATAWAEE68 pKa = 3.89 LNASYY73 pKa = 11.14 AEE75 pKa = 3.9 KK76 pKa = 10.08 WPNITRR82 pKa = 11.84 KK83 pKa = 8.19 GTPPADD89 pKa = 3.35 AEE91 pKa = 4.19 EE92 pKa = 4.5 WKK94 pKa = 10.67 DD95 pKa = 3.77 KK96 pKa = 10.67 PGKK99 pKa = 8.94 TDD101 pKa = 3.24 MLSPEE106 pKa = 3.93 PHH108 pKa = 6.73 KK109 pKa = 11.19 AA110 pKa = 3.16
Molecular weight: 12.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.961
IPC2_protein 4.253
IPC_protein 4.164
Toseland 3.986
ProMoST 4.291
Dawson 4.126
Bjellqvist 4.279
Wikipedia 4.012
Rodwell 3.999
Grimsley 3.897
Solomon 4.113
Lehninger 4.075
Nozaki 4.24
DTASelect 4.406
Thurlkill 4.012
EMBOSS 4.024
Sillero 4.279
Patrickios 1.074
IPC_peptide 4.126
IPC2_peptide 4.266
IPC2.peptide.svr19 4.178
Protein with the highest isoelectric point:
>tr|A0A1Y3G7R4|A0A1Y3G7R4_9PROT Glycosyl transferase OS=Acetobacter sp. DmW_043 OX=1670658 GN=HK11_09915 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 8.99 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.7 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.32 VIANRR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.46 GRR39 pKa = 11.84 KK40 pKa = 8.72 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2062
0
2062
677384
33
2121
328.5
35.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.98 ± 0.055
1.084 ± 0.019
5.222 ± 0.04
5.424 ± 0.064
3.729 ± 0.035
7.943 ± 0.053
2.41 ± 0.022
5.301 ± 0.043
3.707 ± 0.037
10.286 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.512 ± 0.023
3.048 ± 0.036
5.159 ± 0.041
3.756 ± 0.035
6.267 ± 0.055
6.407 ± 0.045
5.889 ± 0.039
7.165 ± 0.044
1.313 ± 0.021
2.396 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here