Gordonia phage GMA2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 126 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K0N726|A0A0K0N726_9CAUD Uncharacterized protein OS=Gordonia phage GMA2 OX=1647283 GN=GMA2_97 PE=4 SV=1
MM1 pKa = 7.38EE2 pKa = 4.93KK3 pKa = 10.46AVTEE7 pKa = 4.27LSSFPVDD14 pKa = 3.77DD15 pKa = 3.96ATLMTVLHH23 pKa = 6.39SLDD26 pKa = 3.29GALNYY31 pKa = 9.58DD32 pKa = 3.68ALGDD36 pKa = 3.89CSLIGSDD43 pKa = 3.45MSFPQLLEE51 pKa = 4.05FLSGPSDD58 pKa = 3.28HH59 pKa = 7.05TEE61 pKa = 3.88YY62 pKa = 9.9EE63 pKa = 4.08TDD65 pKa = 3.08AGTVEE70 pKa = 4.0IRR72 pKa = 11.84EE73 pKa = 4.27DD74 pKa = 3.34PAYY77 pKa = 11.05SSDD80 pKa = 3.58DD81 pKa = 3.74VIRR84 pKa = 11.84ALILEE89 pKa = 4.17IFEE92 pKa = 4.56LRR94 pKa = 11.84GVSDD98 pKa = 3.97NLDD101 pKa = 3.17

Molecular weight: 11.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K0N7J4|A0A0K0N7J4_9CAUD Uncharacterized protein OS=Gordonia phage GMA2 OX=1647283 GN=GMA2_103 PE=4 SV=1
MM1 pKa = 7.69RR2 pKa = 11.84RR3 pKa = 11.84LKK5 pKa = 9.58MRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84ARR11 pKa = 11.84KK12 pKa = 8.94IFYY15 pKa = 10.45ARR17 pKa = 11.84AAYY20 pKa = 9.76EE21 pKa = 4.21SGDD24 pKa = 3.94SVLGRR29 pKa = 11.84SLEE32 pKa = 4.02NEE34 pKa = 3.19IRR36 pKa = 11.84AEE38 pKa = 4.0LGQEE42 pKa = 4.57PIRR45 pKa = 11.84DD46 pKa = 3.91ANDD49 pKa = 3.79SIGLMQQVSTAWKK62 pKa = 9.79RR63 pKa = 11.84PP64 pKa = 3.36

Molecular weight: 7.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

126

0

126

30914

37

2408

245.3

27.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.526 ± 0.283

0.906 ± 0.098

6.861 ± 0.133

6.434 ± 0.243

3.109 ± 0.122

6.935 ± 0.355

1.976 ± 0.151

5.066 ± 0.175

5.376 ± 0.281

8.106 ± 0.188

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.413 ± 0.086

3.61 ± 0.172

4.891 ± 0.164

3.183 ± 0.138

5.988 ± 0.241

7.715 ± 0.246

6.476 ± 0.22

6.803 ± 0.172

1.879 ± 0.083

2.746 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski