Streptococcus phage Javan254

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AVD2|A0A4D6AVD2_9CAUD Uncharacterized protein OS=Streptococcus phage Javan254 OX=2548074 GN=Javan254_0038 PE=4 SV=1
MM1 pKa = 7.42LNKK4 pKa = 9.92EE5 pKa = 4.04STMCDD10 pKa = 3.2VSDD13 pKa = 3.57MAEE16 pKa = 3.96PCNTFDD22 pKa = 3.95EE23 pKa = 4.86LKK25 pKa = 10.72EE26 pKa = 3.76EE27 pKa = 4.8LIDD30 pKa = 4.05YY31 pKa = 9.22FRR33 pKa = 11.84LDD35 pKa = 3.41EE36 pKa = 4.26LL37 pKa = 4.6

Molecular weight: 4.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AWG2|A0A4D6AWG2_9CAUD Terminase large subunit OS=Streptococcus phage Javan254 OX=2548074 GN=Javan254_0024 PE=4 SV=1
MM1 pKa = 7.74ADD3 pKa = 2.91VRR5 pKa = 11.84VAVDD9 pKa = 3.41LAGVEE14 pKa = 4.62KK15 pKa = 10.5KK16 pKa = 10.72VSPQAMQRR24 pKa = 11.84GKK26 pKa = 9.98IAAGNEE32 pKa = 3.63ALLIMDD38 pKa = 3.98SSVPLRR44 pKa = 11.84AGGGALRR51 pKa = 11.84ASGRR55 pKa = 11.84VEE57 pKa = 3.84PNGDD61 pKa = 2.84VSYY64 pKa = 9.06NTVYY68 pKa = 10.97ARR70 pKa = 11.84AQFYY74 pKa = 7.49GTNGIVVFRR83 pKa = 11.84KK84 pKa = 8.49YY85 pKa = 6.45TTPGTGKK92 pKa = 10.34RR93 pKa = 11.84WDD95 pKa = 4.04KK96 pKa = 10.35PLKK99 pKa = 10.92ANIEE103 pKa = 4.2KK104 pKa = 10.5LKK106 pKa = 10.54KK107 pKa = 10.15AAIKK111 pKa = 10.82GMGIRR116 pKa = 4.21

Molecular weight: 12.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

11329

37

1278

222.1

24.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.803 ± 0.917

0.441 ± 0.08

6.373 ± 0.406

6.408 ± 0.553

4.149 ± 0.199

7.265 ± 0.555

1.298 ± 0.149

6.911 ± 0.279

7.6 ± 0.485

7.635 ± 0.26

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.551 ± 0.231

5.411 ± 0.404

2.975 ± 0.165

4.325 ± 0.238

4.299 ± 0.246

6.364 ± 0.415

6.488 ± 0.366

6.461 ± 0.286

1.139 ± 0.143

4.105 ± 0.344
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski